Title:
METHODS FOR MAKING AND USING MOLECULAR SWITCHES INVOLVING CIRCULAR PERMUTATION
Document Type and Number:
WIPO Patent Application WO2005072392
Kind Code:
A3
Abstract:
The invention provides molecular switches which couple external signals to functionality, and combinatorial methods of making and using the same involving circular permutation of nucleic acid and amino acid sequences. The switches according to the invention can be used, for example, to regulate gene transcription, target drug delivery to specific cells, transport drugs intracellularly, control drug release, provide conditionally active proteins, perform metabolic engineering, and modulate cell signaling pathways. Libraries comprising the switches, expression vectors and host cells for expressing the switches are also provided.
Inventors:
OSTERMEIER MARC ALAN (US)
GUNTAS GURKAN (US)
GUNTAS GURKAN (US)
Application Number:
PCT/US2005/002633
Publication Date:
January 05, 2006
Filing Date:
January 28, 2005
Export Citation:
Assignee:
UNIV JOHNS HOPKINS
OSTERMEIER MARC ALAN (US)
GUNTAS GURKAN (US)
OSTERMEIER MARC ALAN (US)
GUNTAS GURKAN (US)
International Classes:
C12N15/62; (IPC1-7): C12N15/66; C12P21/02
Other References:
GUNTAS G. ET AL: "A Molecular Switch Created by in Vitro Recombination of Nonhomologous Genes.", CHEMISTRY & BIOLOGY., vol. 11, no. 11, November 2004 (2004-11-01), pages 1483 - 1487, XP004651202
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