Title:
METHODS FOR TISSUE DECELLULARIZATION
Document Type and Number:
WIPO Patent Application WO/2011/132089
Kind Code:
A3
Abstract:
A method for producing sterile, decellurized bioprosthetic tissue comprising: (a) contacting a human tissue with a hypotonic solution to produce a lysed tissue, (b) contacting the lysed tissue with a first surfactant to produce a surfactant-treated tissue, (c) contacting the surfactant-treated tissue with a nuclease enzyme solution to produce an enzyme-treated tissue, (d) contacting the enzyme-treated tissue with a cleaning solution comprising a second surfactant, a chaotropic agent or a mixture thereof to produce a decellurized tissue and (e) contacting the decellurized tissue with a bioburden reducing agent to produce the final bioprosthetic tissue. Kits to be used in conjunction with said method, as well as, the bioprosthetic tissue produced via said method are also provided.
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Inventors:
GRATZER PAUL FRANK (CA)
Application Number:
PCT/IB2011/001538
Publication Date:
March 01, 2012
Filing Date:
February 25, 2011
Export Citation:
Assignee:
UNIV DALHOUSIE (CA)
GRATZER PAUL FRANK (CA)
GRATZER PAUL FRANK (CA)
International Classes:
A61L27/36; A61L27/60; C07K14/78; C12N1/06; C12N5/071; C12S3/16
Foreign References:
| US20080306610A1 | 2008-12-11 | |||
| US20040076657A1 | 2004-04-22 | |||
| GB2443938A | 2008-05-21 | |||
| CA2396698A1 | 2001-08-02 | |||
| CA2286655A1 | 1998-10-22 |
Other References:
See also references of EP 2538988A4
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