Title:
MODIFIED FIBROIN
Document Type and Number:
WIPO Patent Application WO/2020/145363
Kind Code:
A1
Abstract:
The present invention pertains to a modified fibroin that contains a domain sequence represented by formula 1: [(A)n motif-REP]m or formula 2: [(A)n motif-REP]m-(A)n motif and has a serine residue content of less than 5.5%. [In formulae 1 and 2, (A)n motif represents an amino acid sequence consisting of 4-27 amino acid residues and, in (A) n motif, the ratio of alanine residues is 80% or more relative to the number of total amino acid residues. REP represents an amino acid sequence consisting of 10-200 amino acid residues. m represents an integer of 10-300. A plurality of (A) n motifs may be either the same or different amino acid sequences. A plurality of REPs may be either the same or different amino acid sequences.]
Inventors:
MORITA KEISUKE (JP)
ABE YUNOSUKE (JP)
NAKAMURA HIROYUKI (JP)
TOGASHI SHOTA (JP)
ASAKURA TETSUO (JP)
ABE YUNOSUKE (JP)
NAKAMURA HIROYUKI (JP)
TOGASHI SHOTA (JP)
ASAKURA TETSUO (JP)
Application Number:
PCT/JP2020/000535
Publication Date:
July 16, 2020
Filing Date:
January 09, 2020
Export Citation:
Assignee:
SPIBER INC (JP)
International Classes:
D01F4/02; C07K14/435; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; C12N15/63; C12P21/02
Domestic Patent References:
| WO1991016351A1 | 1991-10-31 | |||
| WO2018164190A1 | 2018-09-13 | |||
| WO2018235958A1 | 2018-12-27 | |||
| WO2018034111A1 | 2018-02-22 | |||
| WO2019022163A1 | 2019-01-31 | |||
| WO2015042164A2 | 2015-03-26 | |||
| WO2013065651A1 | 2013-05-10 | |||
| WO1994023021A1 | 1994-10-13 |
Foreign References:
| JP2014502140A | 2014-01-30 | |||
| JPS63248394A | 1988-10-14 | |||
| JPS58110600A | 1983-07-01 | |||
| JPS60221091A | 1985-11-05 | |||
| US4686191A | 1987-08-11 | |||
| US4939094A | 1990-07-03 | |||
| US5160735A | 1992-11-03 | |||
| JPH0231682A | 1990-02-01 | |||
| JPH04278091A | 1992-10-02 | |||
| JPH07170984A | 1995-07-11 | |||
| JPH06133782A | 1994-05-17 | |||
| JP2002238569A | 2002-08-27 | |||
| JPH02227075A | 1990-09-10 | |||
| JPH05336963A | 1993-12-21 | |||
| JP2009505668A | 2009-02-12 | |||
| JP5678283B2 | 2015-02-25 | |||
| JP4638735B2 | 2011-02-23 |
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BIO/TECHNOLOGY, vol. 6, 1988, pages 47
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCIBIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEMBIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
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NATURE, vol. 195, 1962, pages 788
PAULSON ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 17619
LOWE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 8227
GENES DEVELOP., vol. 4, 1990, pages 1288
See also references of EP 3910096A4
"GenBank", Database accession no. ABR37278.1
"GENBANK", Database accession no. AM490169
PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
GENE, vol. 17, 1982, pages 107
MOLECULAR GENERAL GENETICS, vol. 168, 1979, pages 111
TAKAHASHI ET AL., J. BACTERIOL., vol. 153, 1983, pages 1130 - 1134
TAKAGI ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 3099 - 3100
OKAMOTO, BIOSCI. BIOTECHNOL. BIOCHEM., vol. 61, 1997, pages 202 - 203
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 669
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4306
J. BACTERIOL., vol. 172, 1990, pages 2392
J. BACTERIOL., 1987, pages 1239 - 1245
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413 - 676
APPL. MICROBIOL. BIOTECHNOL., vol. 30, 1989, pages 75 - 80
J. BIOCHEM., vol. 112, 1992, pages 488 - 491
APPL. ENVIRON. MICROBIOL., vol. 58, 1992, pages 525 - 531
J. BACTERIOL., vol. 177, 1995, pages 745 - 749
APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1994, pages 358 - 363
MYERS, A. M. ET AL., GENE, vol. 45, 1986, pages 299 - 310
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI., USA, vol. 81, 1984, pages 4889
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
COHEN ET AL., PROC. NATL. ACAD. SCI, vol. 69, 1972, pages 2110
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
"A Laboratory Manual", 1992, W. H. FREEMAN AND COMPANY, article "Baculovirus Expression Vectors"
"Current Protocols in Molecular Biology", 1992, W. H. FREEMAN AND COMPANY, article "Baculovirus Expression Vectors, A Laboratory Manual"
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCIBIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEMBIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
MOLECULAR CLONING
NATURE, vol. 195, 1962, pages 788
PAULSON ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 17619
LOWE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 8227
GENES DEVELOP., vol. 4, 1990, pages 1288
See also references of EP 3910096A4
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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