Title:
MONOCLONAL ANTIBODY RECOGNIZING SIALYLATED SUGAR CHAINS
Document Type and Number:
WIPO Patent Application WO/2014/178196
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a novel monoclonal antibody having high affinity and that strictly recognizes, as a sugar chain epitope, only a "Siaα2,6Galβ1,4GlcNAc(6'-Sialyl-LacNAc): CDw75" sugar chain structure, being a molecular target for diagnosis of the malignancy of tumors. An anti-CDw75 monoclonal antibody is provided that recognizes "CDw75" sugar chain structures but does not recognize similar sugar chain structures indicated by "Galβ1,4GlcNAc", "Siaα2,3Galβ1,4GlcNAc", or "Siaα2,6Galβ1,4Glc", by using a glycolipid antigen bonding a carrier lipid compound "HOCH2CH(NH-CO-(CH2)22-CH3)-(CH2)9-CH3(C12L)" developed by the inventors to a "CDw75" sugar chain. The obtained anti-CDw75 monoclonal antibody is an excellent detection drug for B-cell lymphoma, gastric cancer, or colorectal cancer, an excellent diagnostic agent for tumor malignancy, etc., an excellent treatment agent for B-cell lymphoma, gastric cancer, or colorectal cancer, and an excellent prevention/treatment drug for influenza.
Inventors:
OKUDA TETSUYA (JP)
Application Number:
PCT/JP2014/002402
Publication Date:
November 06, 2014
Filing Date:
May 02, 2014
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
International Classes:
A61K39/395; C07K16/18; A61P35/00; C12N5/10; G01N33/53; G01N33/574; G01N33/577; C12P21/08
Foreign References:
| JP2002528037A | 2002-08-27 |
Other References:
MURAKAMI D. ET AL.: "Convenient preparation and characterization of a monoclonal a ntibody for the N-linked sugar chain of a glycoprotein using a microbial endoglycosidase.", ARCH. BIOCHEM. BIOPHYS., vol. 477, no. 2, 2008, pages 299 - 304, XP024521354
BAST B.J. ET AL.: "The HB-6, CDw75, and CD 76 differentiation antigens are unique cell - surface carbohydrate determinants generated by the beta-galactoside alpha 2,6- sialyltransferase.", J. CELL. BIOL., vol. 116, no. 2, 1992, pages 423 - 435, XP055294443
COSTA-NOGUEIRA C. ET AL.: "Synthesis and expression of CDw75 antigen in human colorectal cancer.", BMC CANCER, vol. 9, 2009, XP021066998
SUZUKI Y., BIOL. PHARM. BULL., vol. 28, 2005, pages 399 - 408
GUY K.; ANDREW JM., IMMUNOLOGY, vol. 74, 1991, pages 206 - 214
COSTA-NOGUEIRA C. ET AL., BMC CANCER., vol. 9, 2009, pages 431
BAST BJ. ET AL., J. CELL BIOL., vol. 116, 1992, pages 423 - 435
OZAWA H. ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 342, 1997, pages 48 - 57
MURAKAMI D. ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 477, 2008, pages 299 - 304
SHEPHERD P.; DEAN C.: "Monoclonal Antibodies", 2000, OXFORD UNIVERSITY PRESS
BRODIN, EUR. J. IMMUNOL., vol. 16, 1986, pages 951 - 956
FRIGUET B. ET AL., J. IMMUNOL. METHODS, vol. 77, 1985, pages 305 - 319
SHIMIZU H. ET AL., BIOSCI. BIOTECH. BIOCHEM., vol. 60, 1996, pages 73 - 76
BAST B.J. ET AL.: "The HB-6, CDw75, and CD 76 differentiation antigens are unique cell - surface carbohydrate determinants generated by the beta-galactoside alpha 2,6- sialyltransferase.", J. CELL. BIOL., vol. 116, no. 2, 1992, pages 423 - 435, XP055294443
COSTA-NOGUEIRA C. ET AL.: "Synthesis and expression of CDw75 antigen in human colorectal cancer.", BMC CANCER, vol. 9, 2009, XP021066998
SUZUKI Y., BIOL. PHARM. BULL., vol. 28, 2005, pages 399 - 408
GUY K.; ANDREW JM., IMMUNOLOGY, vol. 74, 1991, pages 206 - 214
COSTA-NOGUEIRA C. ET AL., BMC CANCER., vol. 9, 2009, pages 431
BAST BJ. ET AL., J. CELL BIOL., vol. 116, 1992, pages 423 - 435
OZAWA H. ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 342, 1997, pages 48 - 57
MURAKAMI D. ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 477, 2008, pages 299 - 304
SHEPHERD P.; DEAN C.: "Monoclonal Antibodies", 2000, OXFORD UNIVERSITY PRESS
BRODIN, EUR. J. IMMUNOL., vol. 16, 1986, pages 951 - 956
FRIGUET B. ET AL., J. IMMUNOL. METHODS, vol. 77, 1985, pages 305 - 319
SHIMIZU H. ET AL., BIOSCI. BIOTECH. BIOCHEM., vol. 60, 1996, pages 73 - 76
Attorney, Agent or Firm:
SAEKI, Norio et al. (JP)
Norio Saeki (JP)
Norio Saeki (JP)
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