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Title:
A MULTI-PLASMID METHOD FOR PREPARING LARGE LIBRARIES OF POLYKETIDES AND NON-RIBOSOMAL PEPTIDES
Document Type and Number:
WIPO Patent Application WO2000063361
Kind Code:
A3
Abstract:
A multiple-plasmid system for heterologous expression of polyketides facilitates combinatorial biosynthesis. The method can be extended to any modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) and has the potential to produce thousands of novel natural products, including ones derived from further modification of the PKS or NRPS products by tailoring enzymes.

Inventors:
SANTI DANIEL V
XUE QUN
ASHLEY GARY
Application Number:
PCT/US2000/010021
Publication Date:
July 12, 2001
Filing Date:
April 12, 2000
Export Citation:
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Assignee:
KOSAN BIOSCIENCES INC (US)
International Classes:
C12N15/09; A61K31/7048; A61P3/06; A61P31/04; A61P37/06; C07H17/08; C12N15/52; C12P17/08; C12P19/62; C12P21/02; C07B61/00; (IPC1-7): C12N15/10; C12N15/52; C12N9/10; C12N15/63; C12N15/90; C12P19/62; C12R1/465; C07D309/00; C07D313/04; C07D493/10; C07H17/04; A61K31/70; A61P31/00
Domestic Patent References:
WO1998049315A21998-11-05
WO1997002358A11997-01-23
WO1996040968A11996-12-19
WO1999005283A21999-02-04
WO2000024907A22000-05-04
WO2000026349A22000-05-11
WO2000031247A22000-06-02
WO2000052152A12000-09-08
Foreign References:
Other References:
C. RICHARD HUTCHINSON: "Microbial polyketide syntases: more and more prolific", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 3336 - 3338, XP002151574
MCDANIEL ROBERT ET AL: "Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 5, 2 March 1999 (1999-03-02), March 2, 1999, pages 1846 - 1851, XP002143433, ISSN: 0027-8424
KHOSLA C ET AL: "Generation of polyketide libraries via combinatorial biosynthesis", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 14, no. 9, 1 September 1996 (1996-09-01), pages 335 - 341, XP004035738, ISSN: 0167-7799
LIU L ET AL: "Biosynthesis of 2-nor-6-deoxyerythronolide B by rationally designed domain substitutions", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC,US, vol. 119, no. 43, 29 October 1997 (1997-10-29), pages 10553 - 10554, XP002143432, ISSN: 0002-7863
DONADIO S ET AL: "MODULAR ORGANIZATION OF GENES REQUIRED FOR COMPLEX POLYKETIDE BIOSYNTHESIS", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 252, 3 May 1991 (1991-05-03), pages 675 - 679, XP002035890, ISSN: 0036-8075
RUAN X ET AL: "Acyltransferase domain substitutions in erythromycin polyketide synthase yield novel erythromycin derivatives", JOURNAL OF BACTERIOLOGY,US,WASHINGTON, DC, vol. 179, no. 20, October 1997 (1997-10-01), pages 6416 - 6425, XP002117397, ISSN: 0021-9193
KAO C M ET AL: "ENGINEERED BIOSYNTHESIS OF A COMPLETE MACROLACTONE IN A HETEROLOGOUS HOST", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 265, no. 5171, 22 July 1994 (1994-07-22), pages 509 - 512, XP002073974, ISSN: 0036-8075
XUE YONGQUAN ET AL: "A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 95, no. 21, 13 October 1998 (1998-10-13), pages 12111 - 12116, XP002117399, ISSN: 0027-8424
GRAZIANI E I ET AL: "Macrolide biosynthesis: a single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in streptomyces venezuelae", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,GB,OXFORD, vol. 8, no. 22, 17 November 1998 (1998-11-17), pages 3117 - 3120, XP004143711, ISSN: 0960-894X
BETLACH M C ET AL: "Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin", BIOCHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. EASTON, PA, vol. 37, no. 42, 20 October 1998 (1998-10-20), pages 14937 - 14942, XP002117398, ISSN: 0006-2960
OLANO C ET AL: "Analysis of a Streptomyces antibioticus chromosomal region involved in oleandomycin biosynthesis, which encodes two glycosyltransferases responsible for glycosylation of the macrolactone ring", MOLECULAR AND GENERAL GENETICS,DE,SPRINGER VERLAG, BERLIN, vol. 259, no. 3, 1 August 1998 (1998-08-01), pages 299 - 308, XP002096258, ISSN: 0026-8925
QUIROS ET AL: "Biosynthesis of the macrolide oleandomycin by Streptomyces antibioticus", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 270, no. 31, 4 August 1995 (1995-08-04), pages 18234 - 18239, XP002096256, ISSN: 0021-9258
SCHNEIDER A ET AL: "Targeted alteration of the substrate specificity of peptide synthetases by rational module swapping", MOLECULAR AND GENERAL GENETICS,DE,SPRINGER VERLAG, BERLIN, vol. 257, no. 3, February 1998 (1998-02-01), pages 308 - 318, XP002113933, ISSN: 0026-8925
MARAHIEL M A ET AL: "Modular ppetide synthetases involved in nonribosomal peptide synthesis", CHEMICAL REVIEWS,US,AMERICAN CHEMICAL SOCIETY. EASTON, vol. 97, no. 7, November 1997 (1997-11-01), pages 2651 - 2673, XP002133489, ISSN: 0009-2665
TANG LI ET AL: "Cloning and heterologous expression of the epothilone gene cluster", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, vol. 287, 28 January 2000 (2000-01-28), pages 640 - 642, XP002135841, ISSN: 0036-8075
TANG L ET AL: "FORMATION OF FUNCTIONAL HETEROLOGOUS COMPLEXES USING SUBUNITS FROM THE PICROMYCIN, ERYTHROMYCIN AND OLEANDOMYCIN POLYKETIDE SYNTHASES", CHEMISTRY AND BIOLOGY,GB,CURRENT BIOLOGY, LONDON, vol. 7, no. 2, February 2000 (2000-02-01), pages 77 - 84, XP000909347, ISSN: 1074-5521
XUE QUN ET AL: "A multiplasmid approach to preparing large libraries of polyketides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 21, 12 October 1999 (1999-10-12), Oct. 12, 1999, pages 11740 - 11745, XP002151575, ISSN: 0027-8424
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