Title:
MULTISPECIFIC BINDING PROTEIN OF IMMUNE CELL ENGAGER, PREPARATION THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/002038
Kind Code:
A1
Abstract:
Provided are a binding protein and an application thereof. The binding protein comprises a first protein function region bound to a targeting domain of an antigen on a target cell, and a second protein function region comprising an effect domain bound to an antigen on an immune effector cell, and is selected from the following structure: the first protein function region is a Fab structure; the second protein function region is a VH structure or a Fab structure; the binding protein comprises the first and second protein function regions from an N-terminus to a C-terminus in sequence. The binding protein can bring immune effector cells and specific target cells closer together, and efficiently and selectively activate the immune effector cells in the microenvironment of specific target cells, thereby enhancing an immune response and reducing the toxic side effects caused by non-specific activation of peripheral immune cells. The binding protein utilizes the cross-linking of costimulatory molecules mediated by target cell antigens and the activation of downstream signal transduction pathways to promote the activation and proliferation of immune cells and improve the effectiveness in killing tumors.
Inventors:
HE YUN (CN)
SHI LEI (CN)
WU XIAODONG (CN)
ZHONG CHEN (CN)
HUANG BING (CN)
RONG YIPING (CN)
SHI LEI (CN)
WU XIAODONG (CN)
ZHONG CHEN (CN)
HUANG BING (CN)
RONG YIPING (CN)
Application Number:
PCT/CN2021/103065
Publication Date:
January 06, 2022
Filing Date:
June 29, 2021
Export Citation:
Assignee:
HARBOUR BIOMED SHANGHAI CO LTD (CN)
International Classes:
C07K16/46; A61K39/395; A61P35/00; C12N15/13
Domestic Patent References:
| WO2016202457A1 | 2016-12-22 | |||
| WO2020007240A1 | 2020-01-09 | |||
| WO2019184909A1 | 2019-10-03 | |||
| WO2007096779A2 | 2007-08-30 | |||
| WO2015103072A1 | 2015-07-09 |
Foreign References:
| CN111138544A | 2020-05-12 | |||
| CN111138545A | 2020-05-12 | |||
| CN111138542A | 2020-05-12 |
Other References:
NESSPOR THOMAS C., KINEALY KYLE, MAZZANTI NICHOLAS, DIEM MICHAEL D., BOYE KEVIN, HOFFMAN HUNTER, SPRINGER CHRISTINE, SPRENKLE JUST: "High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), pages 7557, XP055883920, DOI: 10.1038/s41598-020-64536-w
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
J MOL BIOL, vol. 273, 1997, pages 927 - 948
See also references of EP 4174092A4
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
J MOL BIOL, vol. 273, 1997, pages 927 - 948
See also references of EP 4174092A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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