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Title:
MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE
Document Type and Number:
WIPO Patent Application WO/2013/077432
Kind Code:
A1
Abstract:
The present invention addresses the following problems: providing an inexpensive, efficient lignocellulose saccharification method using a thermostable xylanase; and providing a mutant xylanase that has a substituted amino-acid residue, exhibits stable activity even under harsh conditions in which enzymes are easily deactivated, and has an initial reaction rate not much less than that of the corresponding wild-type xylanase. A lignocellulose saccharification method that includes bringing a lignocellulose feedstock into contact with a thermostable xylanase; and a mutant xylanase that has a substituted amino-acid residue, has an initial reaction rate at least 70% of that of the corresponding wild-type xylanase, and, after a 24-hour heat treatment at 50°C, exhibits xylanase activity at least 50% of that exhibited before said heat treatment.

Inventors:
YANAI HISAAKI (JP)
TAMAI HIROKI (JP)
OSABE MASAMI (JP)
YOKOYAMA FUMIKAZU (JP)
OKAKURA KAORU (JP)
INOUE ATSUSHI (JP)
Application Number:
PCT/JP2012/080387
Publication Date:
May 30, 2013
Filing Date:
November 22, 2012
Export Citation:
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Assignee:
MITSUI CHEMICALS INC (JP)
MEIJI SEIKA PHARMA CO LTD (JP)
International Classes:
C12N15/56; A21D8/04; A23K1/165; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N9/24; C12P19/14; D06L4/40
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Foreign References:
JPH10215866A1998-08-18
JP2000166485A2000-06-20
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JP2011045277A2011-03-10
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Other References:
DATABASE GENBANK [online] 14 November 2006 (2006-11-14), ALCOCER,M.J. ET AL.: "ENDO-1,4-XYLANASE B [TALAROMYCES FUNICULOSUS]", XP055177435, retrieved from NCBI Database accession no. CAD33900
See also references of EP 2784161A4
KRAMER, W.; FRITA, H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
"PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.; FRITA H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
VAN DEN HONDEL; C. A. M. J. J. ET AL.: "More gene Manipulations in Fungi", 1991, ACADEMIC PRESS, pages: 396 - 428
SAMBROOK, J.: "Molecular Cloning A Laboratory Manual, 3rd Edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
Nakajima 淳 (JP)
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Claims: