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Title:
NON-NATURAL LABELED AMINO ACID AND METHOD OF CONSTRUCTING AMINO ACID/tRNA COMPLEX
Document Type and Number:
WIPO Patent Application WO/2004/009709
Kind Code:
A1
Abstract:
A labeled amino acid which can be transferred into a protein with the use of a protein synthesis system and a functional protein having a function originating in a labeling compound. A labeled amino acid wherein an aromatic ring is bonded to a side chain of the amino acid and a labeling compound is further bonded to the amino acid via the aromatic ring; and a functional protein having the labeled amino acid transferred thereinto. Also, a novel method for efficiently obtaining a labeled amino acid/tRNA complex is provided.

Inventors:
HOHSAKA TAKAHIRO (JP)
SISIDO MASAHIKO (JP)
Application Number:
PCT/JP2003/008970
Publication Date:
January 29, 2004
Filing Date:
July 15, 2003
Export Citation:
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Assignee:
PROTEIN EXPRESS CO LTD (JP)
HOHSAKA TAKAHIRO (JP)
SISIDO MASAHIKO (JP)
International Classes:
C07F5/02; C09B57/00; C09B57/10; (IPC1-7): C09B57/00; C07F5/02; C07K1/13; C07K16/40; C12N15/09
Domestic Patent References:
WO2000013026A12000-03-09
Foreign References:
JPH10330299A1998-12-15
JP2001139596A2001-05-22
Other References:
HOHSAKA T. ET AL.: "Efficient incorporationof nonnatural amino acids with large aromatic groups into streptavidin in in vitro protein synthesizing systems", J. AM. CHEM. SOC., vol. 121, no. 1, 1999, pages 34 - 40, XP000953102
HOHSAKA T. ET AL.: "Incorporation of nonnatural amino acids into streptavidin through in vitro frame-shift suppression", J. AM. CHEM. SOC., vol. 118, no. 40, 1996, pages 9778 - 9779, XP002973328
HIRAO I. ET AL.: "An unnatural base pair for incorporating amino acid analogs into proteins", NATURE BIOTECHNOLOGY, vol. 20, February 2002 (2002-02-01), pages 177 - 182, XP001068466
Attorney, Agent or Firm:
Hiraki, Yusuke (17-1 Toranomon 1-chom, Minato-ku Tokyo, JP)
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