Title:
NOVEL ENZYME FOR PRODUCING D-THREONINE, AND METHOD FOR STEREOSPECIFICALLY PRODUCING D-THREONINE BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/080277
Kind Code:
A1
Abstract:
The present invention relates to a novel enzyme for producing D-threonine. When reactions are carried out by using the enzyme of the present invention, the ratio of diastereomer D-allo-threonine, which can be produced together with D-threonine, is lowered and higher purity D-threonine can be obtained.
Inventors:
LEE SEUNG GOO (KR)
YEOM SOO JIN (KR)
KIM HA SEONG (KR)
PARK SUNG HYUN (KR)
LEE DAE HEE (KR)
RHA EU GENE (KR)
LEE HYE WON (KR)
KWON KIL KOANG (KR)
AN JUNG UNG (KR)
YEOM SOO JIN (KR)
KIM HA SEONG (KR)
PARK SUNG HYUN (KR)
LEE DAE HEE (KR)
RHA EU GENE (KR)
LEE HYE WON (KR)
KWON KIL KOANG (KR)
AN JUNG UNG (KR)
Application Number:
PCT/KR2020/014292
Publication Date:
April 29, 2021
Filing Date:
October 20, 2020
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
C12N9/88; C12N15/70; C12P13/08
Other References:
DATABASE Protein GenBank; ANONYMOUS: "MULTISPECIES: DSD1 family PLP-dependent enzyme [Filomicrobium]", XP055805805, retrieved from NCBI
SUNG-HYUN PARK, SOO-JIN YEOM, SEUNG-GOO LEE: "Characterization of A New D-Threonine Aldolase for D-Threonone Production.", 2019 KSBB SPRING MEETING AND INTERNATIONAL SYMPOSIUM; 15-17 APRIL 2015, 1 April 2019 (2019-04-01), Korea, pages 554, XP009527661
DATABASE Nucleotide GenBank; ANONYMOUS: "Candidatus Filomicrobium marinum strain Y genome assembly, chromosome: 1", XP055805807, retrieved from NCBI
QIJIA CHEN, CHEN XI, CUI YUNFENG, REN JIE, LU WEI, FENG JINHUI, WU QIAQING, ZHU DUNMING: "A new D-threonine aldolase as a promising biocatalyst for highly stereoselective preparation of chiral aromatic β-hydroxy-α-amino acids", CATALYSIS SCIENCE & TECHNOLOGY, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 7, no. 24, 1 January 2017 (2017-01-01), UK, pages 5964 - 5973, XP055695514, ISSN: 2044-4753, DOI: 10.1039/C7CY01774J
HENRIQUES ANA C., DE MARCO PAOLO: "Complete Genome Sequences of Two Strains of “ Candidatus Filomicrobium marinum,” a Methanesulfonate-Degrading Species", GENOME ANNOUNCEMENTS, vol. 3, no. 3, 25 June 2015 (2015-06-25), XP055805802, DOI: 10.1128/genomeA.00160-15
SUNG-HYUN PARK, SOO-JIN YEOM, SEUNG-GOO LEE: "Characterization of A New D-Threonine Aldolase for D-Threonone Production.", 2019 KSBB SPRING MEETING AND INTERNATIONAL SYMPOSIUM; 15-17 APRIL 2015, 1 April 2019 (2019-04-01), Korea, pages 554, XP009527661
DATABASE Nucleotide GenBank; ANONYMOUS: "Candidatus Filomicrobium marinum strain Y genome assembly, chromosome: 1", XP055805807, retrieved from NCBI
QIJIA CHEN, CHEN XI, CUI YUNFENG, REN JIE, LU WEI, FENG JINHUI, WU QIAQING, ZHU DUNMING: "A new D-threonine aldolase as a promising biocatalyst for highly stereoselective preparation of chiral aromatic β-hydroxy-α-amino acids", CATALYSIS SCIENCE & TECHNOLOGY, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 7, no. 24, 1 January 2017 (2017-01-01), UK, pages 5964 - 5973, XP055695514, ISSN: 2044-4753, DOI: 10.1039/C7CY01774J
HENRIQUES ANA C., DE MARCO PAOLO: "Complete Genome Sequences of Two Strains of “ Candidatus Filomicrobium marinum,” a Methanesulfonate-Degrading Species", GENOME ANNOUNCEMENTS, vol. 3, no. 3, 25 June 2015 (2015-06-25), XP055805802, DOI: 10.1128/genomeA.00160-15
Attorney, Agent or Firm:
BAE, KIM & LEE IP (KR)
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