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Title:
NOVEL FLUORESCENT PROTEIN AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/066157
Kind Code:
A1
Abstract:
In this fluorescent protein, in the amino acid sequence of green fluorescent protein (GFP) derived from Aequorea victoria, or a variant fluorescent protein of said green fluorescent protein, the amino acid residue (alanine residue) corresponding to position 206 using the amino acid sequence of GFP as a reference sequence is substituted with a phenylalanine residue.

Inventors:
NIINO YUSUKE (JP)
MIYAWAKI ATSUSHI (JP)
Application Number:
PCT/JP2020/037578
Publication Date:
April 08, 2021
Filing Date:
October 02, 2020
Export Citation:
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Assignee:
RIKEN (JP)
International Classes:
C07K14/435; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; C12N15/63; C12Q1/02; G01N33/542
Domestic Patent References:
WO2006068187A12006-06-29
Foreign References:
JP2002369690A2002-12-24
Other References:
COTLET, MIRCEA, GOODWIN PETER M., WALDO GEOFFREY S., WERNER JAMES H.: "Time-resolved detection of the one- and two-photon excited fluorescence of single molecules of a folding enhanced green fluorescent protein", PROCEEDINGS OF SPIE, vol. 6092, 2006, pages 609204 - 1 - 629204-10, XP055811058, DOI: 10.1117/12.646697
ZAPATA-HOMMER, OTILIA; GRIESBECK OLIVER: "Efficiently folding and circularly permuted variants of the sapphire mutant of GFP", BMC BIOTECHNOLOGY, vol. 3, no. 5, 2003, pages 1 - 6, XP021017034, DOI: 10.1186/1472-6750-3-5
EVDOKIMOV, ARTEM G, POKROSS MATTHEW E, EGOROV NIKOLAY S, ZARAISKY ANDREY G, YAMPOLSKY ILYA V, MERZLYAK EKATERINA M, SHKOPOROV ANDR: "Structural basis for the fast maturation of Arthropoda green fluorescent protein", EMBO REPORTS, vol. 7, no. 10, 2006, pages 1006 - 1012, XP055811060, DOI: 10.1038/sj.embor.7400787
SAWANO, A. ET AL.: "Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis", NUCLEIC ACIDS RESEARCH, vol. 28, no. 16, 2000, pages 1 - 7, XP002353209, DOI: 10.1093/nar/28.16.e78
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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