Title:
NOVEL METHOD FOR MEASURING SFLT-1 (SOLUBLE VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1)
Document Type and Number:
WIPO Patent Application WO/2019/026870
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an sFlt-1 measurement method for sFlt-1 protein, which is novel, simple, inexpensive and suitable for practical use. A method for measuring sFlt-1 in a sample by forming a complex in which sFlt-1 is sandwiched by heparin and an anti-sFlt-1 antibody, the method comprising: bringing heparin or an anti-sFlt-1 antibody immobilized on a support into contact with the sample to form a complex of heparin or the anti-sFlt-1 antibody and sFlt-1 in the sample on the support; and detecting the presence of the complex using a labeled anti-sFlt-1 antibody, or an anti-sFlt-1 antibody and a labeled anti-immunoglobulin antibody bonded to the labeled anti-sFlt-1 antibody, or labeled heparin.
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Inventors:
SHIBUYA MASABUMI (JP)
Application Number:
PCT/JP2018/028541
Publication Date:
February 07, 2019
Filing Date:
July 31, 2018
Export Citation:
Assignee:
GAKUBUNKAN (JP)
International Classes:
G01N33/53; C12M1/34; G01N33/543; G01N33/545; G01N33/566
Domestic Patent References:
| WO2003082258A1 | 2003-10-09 |
Foreign References:
| JP2000009733A | 2000-01-14 | |||
| JP2013532129A | 2013-08-15 | |||
| JPH10239313A | 1998-09-11 | |||
| JPH0315758A | 1991-01-24 |
Other References:
PARK, M ET AL.: "The fourth immunoglobulin-like loop in the extracellular domain of FLT-1, a VEGF receptor, includes a major heparin-binding site", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 264, no. 3, 1 November 1999 (1999-11-01), AMSTERDAM, NL, pages 730 - 734, XP055678613, ISSN: 0006-291X, DOI: 10.1006/bbrc.1999.1580
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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