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Title:
OIL COMBINATIONS WITH COCONUT OIL AND MULTI-PROBIOTIC PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/221589
Kind Code:
A1
Abstract:
A probiotic and oil combination developed for use in health sector in the treatment and/or preventive treatments for Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition consisting of probiotic bacteria Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacteriim bifidum, Bifidobacterium longum, Bacillus licheniformis, Bacillus clausii, Faecalibacterium prausnitzii and coconut oil selected as fixed oil forming oily carriers, patchouli oil selected as essential oil, peppermint oil, lemon oil and a production method thereof.

Inventors:
TARHAN ÇELEBİ LEYLA (TR)
ÇAKICI BEKIR (TR)
ASLAN İSMAIL (TR)
KAYAN HÜLYA (TR)
Application Number:
PCT/TR2021/050390
Publication Date:
November 04, 2021
Filing Date:
April 27, 2021
Export Citation:
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Assignee:
HLY AROMATERAPI HIZMETLERI KOZMETIK SANAYI TICARET ANONIM SIRKETI (TR)
SFA ARGE VE OEZEL SAGLIK HIZMETLERI TICARET LTD SIRKETI (TR)
International Classes:
A23D9/00; A23L33/135; C12N1/00
Domestic Patent References:
WO2010122107A12010-10-28
Other References:
SARKAR A ET AL.: "Psychobiotics and the Manipulation of Bacteria-Gut-Brain Signals", TRENDS NEUROSCI, vol. 39, no. 11, 2016, pages 763 - 781, XP029802741, DOI: 10.1016/j.tins. 2016.09.00 2
Attorney, Agent or Firm:
DESTEK PATENT, INC. (TR)
Download PDF:
Claims:
CLAIMS

1. A probiotic and oil combination developed for use in the treatment and/or preventive treatments for Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition characterized in comprising probiotic bacteria Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis , Bifidobacteriim bifidum , Bifidobacterium longum , Bacillus licheniformis, Bacillus clausii, Faecalibacterium prausnitzii and coconut oil, patchouli oil, mint oil and lemon oil .

2. The probiotic and oil combination according to claim 1 , characterized in comprising the following in the total mixture by weight, 95-97% coconut oil, 0,23-0,27% patchouli oil, 0,22-0,26% mint oil, 0,22-0,24% lemon oil, 0,450-0,550% Lactobacillus rhamnosus, 0,150-0,250% Lactobacillus plantarum, 0,450-0,550% Lactobacillus gastricus, 0,450- 0,550% Lactobacillus acidophilus, 0,150-0,250% Bifidobacterium animalis, 0,280 - 0,320% Bifidobacterium bifidum, 0,450-0,550% Bifidobacterium longum, 0,280 - 0,320% Bacillus licheniformis, 0,150 - 0,250% Bacillus clausii, 0,450-0,550% Faecalibacterium prausnitzii .

3. The probiotic and oil combination according to claim 2, characterized in comprising the following in the total mixture by weight;95,7% coconut oil, 0,26% patchouli oil, 0,25% mint oil, 0,23% lemon oil, 0,5% Lactobacillus rhamnosus, 0,2% Lactobacillus plantarum, 0,5% Lactobacillus gastricus, 0,5% Lactobacillus acidophilus, 0,2% Bifidobacterium animalis, 0.3% Bifidobacterium bifidum, %0,5% Bifidobacterium longum, 0.3% Bacillus licheniformis, 0.2% Bacillus clausii, 0,5% Faecalibacterium prausnitzii .

4. A production method of a probiotic and oil combination, characterized in comprising the following process steps; i. isolating microorganisms in sterile area at 22°C - 45°C temperature, 35-75% humidity and 2 -12 days interval and purifying isolates, homogeneously mixing with trihalose, maltodextrin, platinose, cyclodextrin, dextrose and all kinds of 6- carbon sugar and all prebiotic fibers in the bioreactor, ii. freeze drying of microorganisms in a freezer-freezer lyophilizer in a sterile area for 1 - 5 days at a temperature between -80°c and -20°c, iii. mixing the microorganism combination in the cubic mixer, iv. adding patchouli, mint, lemon oils into coconut oil, v. adding the following bacteria to oil and homogenizing the same in the range of 1-5 days, in the sterile area lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus gastricus, lactobacillus acidophilus, bifidobacterium animalis, bifidobacteriim bifidum, bifidobacterium longum, bacillus licheniformis, bacillus clausii, faecalibacterium prausnitzii, vi. filling of probiotic and oil combination into amber glass bottles with a peristaltic pump.

Description:
OIL COMBINATIONS WITH COCONUT OIL AND MULTI-PROBIOTIC PRODUCTION

METHOD

Technical Field

The present invention relates to a probiotic and an oil combination that is consumed as a supplementary and preventive food to be used as therapeutic and/or preventive food for providing Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition", is developed for use in health and food sector.

The invention particularly relates to a probiotic and oil combination comprising coconut oil, patchouli oil, peppermint oil, lemon oil with probiotic bacteria Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacteriim bifidum, Bifidobacterium longum, Bacillus licheniformi, Bacillus clausii, Faecalibacterium prausnitzii and production method thereof.

Present State of the Art

Recently, as a result of the increased awareness of consumers, the trend regarding health protection by means of nutrition has increased. This condition has revealed the term functional food and its industry. Functional foods are shortly defined as "foods that positively affect health in addition to their nutritional value". Today, foods or supplements containing probiotics establish a substantial part of this sector.

Probiotics use nutrients by attaching to intestinal surfaces and creating an ecological environment for them and thus they restrict the development of pathogens by creating a competitive environment. Furthermore, they prevent the growth of other harmful bacteria by producing various organic acids such as acetic acid and lactic acid, lowering the pH and producing bacteriocins with hydrogen peroxide under certain conditions. The positive effect of probiotics is not limited to the gastrointestinal tract, but it is known that they also provide various nutritional and therapeutic effects. It increases the bioavailability of lipids and proteins by means of the enzymatic hydrolysis that occurs with the participation of these bacteria and they reduce the allergenic property and effect of food products. For example, they can digest lactose and reduce the symptoms caused by lactose intolerance by lowering the level of lactose in fermented dairy products. Furthermore, probiotics are effective in lowering serum cholesterol level, in the treatment and prevention of diarrhea, in the control of Helicobacter pylori infection and inflammatory bowel disease, they also show various anticarcinogenic and antimutagenic effects. It has been reported that probiotics have positive effects in the healing process of wounds, and probiotic use in babies reduces diarrhea, colic pain, allergic diseases and respiratory disorders and strengthens the immune system according to the findings.

Today, although aromatic oils and probiotic contents are used in health, food and cosmetics sectors, there are not enough studies regarding their use in combination. There are various dermo-cosmetic and odor treatment areas where aromatic oils are used. In this field, edible oils are consumed as food supplements depending on their rich content. Furthermore, it is also used in psychosocial cosmetic therapies due to its pleasant odor property. Probiotic ingredients are also widely consumed as food supplements. Some beneficial bacteria and/or yeast combinations are used as drugs for intestinal diseases. However, although there are formulas available to prevent the formation of gastrointestinal (digestive system) disorders brought by nutritional habits of today, they were insufficient.

Coconut oil and selected probiotic combination are considered in the inhibitive and/or preventive approach for Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition.

The family of beneficial bacteria in the content of selected probiotic combination, the metabolic diseases that occur in their deficiencies have been the subject of many studies. There are many studies regarding brain-intestinal line, but the treatments aimed at eliminating the deficiencies in this line have been limited to chemical approaches.

The following applications are encountered in the literature regarding the subject matter.

In the patent application numbered TR2013/14745 with the title "Probiotic composition for use in the treatment of intestinal inflammation.”, it is combined in a formulation using species of the Lactobacillus plantarum family and the Pediococcus acidilactici family, especially in the treatment of gastrointestinal diseases such as intestinal inflammation and irritable bowel syndrome.

In the patent application numbered TR2019/17034 titled "Probiotic production of Bacillus clausii spores in lozenge form”, Bacillus clausii spores are used as throat lozenges as an intestinal and digestive system regulator.

Another invention is the patent application numbered TR 2013/14095 with title "Preparation containing probiotic bacteria and fish oil". Said invention is a probiotic preparation consisting of a powder mixture containing Bifidobacterium Infantis, Bifidobacterium breve and Lactobasillus acidophilus probiotic bacteria, which has a vial liquid containing inulin and is used by pouring the liquid in it, to be used in the elimination of probiotic deficiency and in the proper functioning of children's mental development, strengthening of nervous systems, eye development and energy metabolism, characterized in that, said vial liquid contains fish oil containing Omega-3 fatty acids.

As a result, due to the abovementioned disadvantages and the insufficiency of the current solutions regarding the subject matter, a development is required to be made in the relevant technical field.

Brief Description of the Invention

The present invention is related to probiotic and oil combination which fulfils the abovementioned requirements, eliminate all disadvantages, and bring some additional advantages.

The main aim of the invention is to provide a probiotic and oil combination that is consumed as a supplementary and preventive food to be used as therapeutic and/or inhibitor for providing Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition", is developed for use in health and food sector.

An aim of the invention is to provide a probiotic and oil combination consisting of coconut oil, patchouli oil, peppermint oil, lemon oil with probiotic bacteria Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bacillus licheniformi, Bacillus clausii, Faecalibacterium prausnitzii.

Another aim of the invention is to be used as a therapeutic agent and a supplement in preventive medicine in the preventive and/or inhibitive approach envisaged for reducing or eliminating fungal diseases of the skin or mucosa (mouth, genital area, armpit, etc.) caused by Candida, stopping resistant fungal infections in finger and toenails, correcting intestinal permeability, eliminating developing disorders in case of Sibo (Small Intestine Bacterial Overgrowth) and relieving anxiety that develops due to various reasons (depression, anxiety, anxiety disorders, etc.).

Another aim of the invention is to provide a probiotic product that has no adverse side effects and does not contain harmful chemicals and contributes to human health.

In order to fulfil the above mentioned aims, the invention is a combination of coconut oil, patchouli oil, peppermint oil, lemon oil and selected probiotic to be used in supplementary preventive medicine in the preventive and/or inhibitive approach for metabolic disease, dermatological diseases and immune system envisaged for reducing or eliminating fungal diseases of the skin or mucosa (mouth, genital area, armpit, etc.) caused by Candida, stopping resistant fungal infections in finger and toenails, correcting intestinal permeability, eliminating developing disorders in case of Sibo (Small Intestine Bacterial Overgrowth)and relieving anxiety that develops due to various reasons (depression, anxiety, anxiety disorders, etc.); it comprises Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacteriim bifidum, Bifidobacterium longum, Bacillus licheniformis, Bacillus clausii, Faecalibacterium prausnitzii probiotic bacteria and coconut oil, patchouli oil, mint oil and lemon oil.

The structural and characteristic features of the present invention will be understood clearly by the following detailed description and therefore the evaluation shall be made by taking the detailed description into consideration.

Detailed Description of the Invention

In this detailed description, the inventive probiotic and oil combination is described only for clarifying the subject matter in a manner such that no limiting effect is created.

A probiotic and oil combination developed for use in health sector in the treatment and/or preventive treatments for Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition consisting of probiotic bacteria Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus gastricus, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacteriim bifidum, Bifidobacterium longum, Bacillus licheniformis, Bacillus clausii, Faecalibacterium prausnitzii and coconut oil selected as fixed oil forming oily carriers, patchouli oil as essential oil, peppermint oil, lemon oil and a production method thereof.

Coconut ( Cocos nucifera) contains capric, lauric, myristic, palmitic and stearic acids among saturated fatty acids and are known as medium chain fatty acids. These fatty acids are rich in vitamins C, E, and K, sodium, potassium, niacin, pantothenic acid, pyridoxine, riboflavin, thiamine, they can play a role in regulating the plasma lipid profile, maintaining body weight control, creating protection against some types of cancer, and reducing deoxyribonucleic acid (DNA) damage due to aging by means of their its nutrient and phytochemical content. They have therapeutic effects on brain disorders such as epilepsy and Alzheimer’s disease etc. They control diabetes by improving insulin secretion and blood sugar utilization, and affects digestion and other symptoms associated with digestive and intestinal disorders. Essential oils and aromatic extracts are used in the perfume and flavor industry, in perfumes, food additives, cleaning products, cosmetics and pharmaceutical preparations, as a source of aroma chemicals or as a starting source in the synthesis of nature-identical and semi-synthetic flavor preparates. Aromatherapy is particularly used for indigestion problems, skin diseases, headaches, insomnia, stress, cancer treatment, and improving mental perception. In addition to the antibacterial and antifungal effects of essential oils and aroma extracts, they also have effects on the nervous system, digestive system, immune system and respiratory system. Patchouli oil, mint oil and lemon oil used in health and cosmetic industries as essential oil are used as essential oils.

Table 1. The inventive probiotic and oil combination content

The present invention comprises the following in total mixture by weight; 95-97% coconut oil, 0,23-0,27% patchouli oil, 0,22-0,26% mint oil, 0,22-0,24% lemon oil, 0,450-0,550% Lactobacillus rhamnosus, 0,150-0,250% Lactobacillus plantarum, 0,450-0,550% Lactobacillus gastricus, 0,450-0,550% Lactobacillus acidophilus, 0,150-0,250% Bifidobacterium animalis, 0,280 - 0,320% Bifidobacterium bifidum, 0,450-0,550% Bifidobacterium longum, 0,280 - 0,320% Bacillus licheniformis, 0,150 - 0,250% Bacillus clausii, 0,450-0,550% Faecalibacterium prausnitzii .

A preferred embodiment of the invention comprises the following in the total mixture by weight;95,7% coconut oil, 0,26% patchouli oil, 0,25% mint oil, 0,23% lemon oil, 0,5% Lactobacillus rhamnosus, 0,2% Lactobacillus plantarum, 0,5% Lactobacillus gastricus, 0,5% Lactobacillus acidophilus, 0,2%

Bifidobacterium animalis, 0.3% Bifidobacterium bifidum, %0,5% Bifidobacterium longum, 0.3% Bacillus licheniformis, 0.2% Bacillus clausii, 0,5% Faecalibacterium prausnitzii. The inventive probiotic and oil combination and production method comprises the following process steps;

- isolating microorganisms in sterile area at 22°c - 45°c temperature, 35-75% humidity and 2 -12 days interval and purifying isolates, homogeneously mixing with trihalose, maltodextrin, platinose, cyclodextrin, dextrose and all kinds of 6-carbon sugar and all prebiotic fibers in the bioreactor,

- freeze drying of microorganisms in a freezer-freezer lyophilizer in a sterile area for 1 - 5 days at a temperature between -80°c and -20°c, mixing the microorganism combination in the cubic mixer, - adding patchouli, mint, lemon oils into coconut oil,

- adding the following bacteria to oil and homogenizing the same in the range of 1-5 days, in the sterile area lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus gastricus, lactobacillus acidophilus, bifidobacterium animalis, bifidobacteriim bifidum, bifidobacterium longum, bacillus licheniformis, bacillus clausii, faecalibacterium prausnitzii ,

- filling of probiotic and oil combination into amber glass bottles with a peristaltic pump.

Filled bottles are kept for 72 hours at 25°C for physical control before packaging. The most ideal working environment of the probiotic combination in the invention is the essential and fixed oil mixture as detailed above. These elements are the original and sustainable characteristics of the invention and have an important contribution to the invention process since they have not been made previously. It will be used as a therapeutic agent and supplement in preventive medicine in the predictive and/or preventive approach for Candidiasis regression, Onychomycosis regression, Intestinal permeability regression, Sibo (Small Intestine Bacterial Overgrowth) inhibition, anxiety inhibition.

It is prescribed that individuals who are allergic to any ingredient contained in the invention should not use it. It has no known side effects. It can be consumed 1-2 times a day in the range of 5-10 ml hungry/full. The product is recommended to be consumed in the afternoon. Any bulges, unusual odours and similar situations in the product shall be reported to the authorized persons. The invention can be used until the disorders are eliminated and subsequently can be used for prophylaxis (protective).