Title:
OIL PHASE COMPOSITION FOR PREPARING DIGITAL PCR MICRODROPLETS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/228257
Kind Code:
A1
Abstract:
The present invention provides an oil phase composition for preparing digital PCR microdroplets, consisting of the following components: 1) a macromolecular nonionic surfactant having a low hydrophilic lipophilic balance value and having polydimethylsiloxane as a backbone (2-5%w/w); 2) a polyethyleneglycol micromolecular nonionic surfactant having a high hydrophilic lipophilic balance value (0.05-0.2%w/w); 3) an anti-evaporation agent (1-5%w/w); and 4) the balance a base oil. The digital PCR microdroplets prepared using the oil phase composition provided by the present invention have the advantages of a uniform size, good thermostability, no evaporation, no fusion, and no inhibition of PCR amplification.
Inventors:
WENG RONGRONG (CN)
Application Number:
PCT/CN2019/114819
Publication Date:
November 19, 2020
Filing Date:
October 31, 2019
Export Citation:
Assignee:
BEIJING DAWEI BIOTECH LTD (CN)
International Classes:
C12Q1/68
Foreign References:
| CN110042151A | 2019-07-23 | |||
| CN106755345A | 2017-05-31 |
Attorney, Agent or Firm:
BEIJING TANGSONG IP FIRM (CN)
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