Title:
OLIGONUCLEOTIDE PRODUCTION METHOD, AND NUCLEOSIDE, NUCLEOTIDE, OR OLIGONUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO/2017/104836
Kind Code:
A1
Abstract:
The present invention provides an oligonucleotide production method that includes: (1) a step in which a reaction liquid comprising a phosphite triester body (c) is obtained by condensing, in a nonpolar solvent, a nucleoside, nucleotide, or oligonucleotide (a) in which the 5'-hydroxyl group is not protected and a nucleoside, nucleotide, or oligonucleotide (b) in which the 5'-hydroxyl group is protected; (3) a step in which the phosphite triester body (c) is oxidized or sulfurized and a reaction liquid is obtained that comprises an oligonucleotide (d) in which the 5'-hydroxyl group is protected; (4) a step in which the oligonucleotide (d) is deprotected and a reaction liquid is obtained that comprises an oligonucleotide (e) in which the 5'-hydroxyl group is not protected; and (6) a step in which a polar solvent is added to the reaction liquid comprising the oligonucleotide (e), and the oligonucleotide (e) is purified by solid-liquid separation or extraction.
Inventors:
HIRAI KUNIHIRO (JP)
KATAYAMA SATOSHI (JP)
HIROSE NAOKO (JP)
ICHIMARU TAISUKE (JP)
YAMASHITA KEN (JP)
TAKAHASHI DAISUKE (JP)
KATAYAMA SATOSHI (JP)
HIROSE NAOKO (JP)
ICHIMARU TAISUKE (JP)
YAMASHITA KEN (JP)
TAKAHASHI DAISUKE (JP)
Application Number:
PCT/JP2016/087654
Publication Date:
June 22, 2017
Filing Date:
December 16, 2016
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C07H21/02; C07H19/10; C07H19/20; C12N15/09
Domestic Patent References:
| WO2014077292A1 | 2014-05-22 | |||
| WO2013122236A1 | 2013-08-22 | |||
| WO2012157723A1 | 2012-11-22 | |||
| WO2005070859A1 | 2005-08-04 | |||
| WO2013179412A1 | 2013-12-05 |
Foreign References:
| US20130267697A1 | 2013-10-10 | |||
| JP2010275254A | 2010-12-09 | |||
| JP2012111728A | 2012-06-14 |
Other References:
KIM SHOKAKU ET AL.: "Liquid-Phase RNA Synthesis by Using Alkyl-Chain-Soluble Support", CHEMISTRY - A EUROPEAN JOURNAL, vol. 19, no. 26, 2013, pages 8615 - 8620, XP055211240
SHOJI TAKAO ET AL.: "Synthesis of conjugated oligonucleotide in solution phase using alkyl- chain-soluble support", CHEMISTRY LETTERS, vol. 43, no. 8, 2014, pages 1251 - 1253, XP055393419
SHOJI TAKAO ET AL.: "Synthesis of conjugated oligonucleotide in solution phase using alkyl- chain-soluble support", CHEMISTRY LETTERS, vol. 43, no. 8, 2014, pages 1251 - 1253, XP055393419
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
Download PDF: