Title:
PROCESS FOR PRODUCING ERYSIPELOTHRIX RHUSIOPATHIAE SURFACE PROTECTIVE ANTIGEN MUTANT IN ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2005/083072
Kind Code:
A1
Abstract:
It is intended to provide a mutant of a surface protective antigen SpaA protein of Erysipelothrix rhusiopathiae or a shortened SpaA protein (&Dgr SpaA) obtained by removing a part therefrom and a process for producing the same. By introducing an amino acid substituent into a definite position of the amino acid sequence of the SpaA protein or the &Dgr SpaA protein, a mutant of the SpaA protein or the &Dgr SpaA protein having immunogenicity and being expressed as an inclusion body in Escherichia coli can be obtained. Because of being expressed as an insoluble inclusion body in Escherichia coli, the above-described mutant of the SpaA protein or the &Dgr SpaA protein can be easily collected and purified.
Inventors:
USHIJIMA TOSHIHIRO (JP)
SAKAGUCHI MASASHI (JP)
TOKUNAGA EIJI (JP)
SAKAGUCHI MASASHI (JP)
TOKUNAGA EIJI (JP)
Application Number:
PCT/JP2005/001814
Publication Date:
September 09, 2005
Filing Date:
February 08, 2005
Export Citation:
Assignee:
CHEMO SERO THERAPEUT RES INST (JP)
USHIJIMA TOSHIHIRO (JP)
SAKAGUCHI MASASHI (JP)
TOKUNAGA EIJI (JP)
USHIJIMA TOSHIHIRO (JP)
SAKAGUCHI MASASHI (JP)
TOKUNAGA EIJI (JP)
International Classes:
A61K38/16; A61P31/00; C07K14/195; C12N15/09; C12P21/02; A61K38/00; A61K39/00; (IPC1-7): C12N15/09; C07K14/195; C12P21/02; A61K38/16; A61P31/00
Foreign References:
| JP2002034568A | 2002-02-05 | |||
| JP2000279179A | 2000-10-10 | |||
| JP2002306163A | 2002-10-22 | |||
| JPH0630771A | 1994-02-08 | |||
| JP2000279179A | 2000-10-10 | |||
| JP2002034568A | 2002-02-05 |
Other References:
SHIMOJI Y. ET AL: "Immunological Characterization of a Protective Antigen of Erysipelothrix rhusiopathiae: Identification of the Region Responsible for Protective Immunity", INFECT.IMMUN., vol. 67, 1999, pages 1646 - 1651, XP000914977
IMADA Y. ET AL: "Truncated Surface Protective Antigen (SpaA) of Erysipelothrix rhusiopathiae Serotype la Elicits Protection against challenge with Serotypes 1a and 2b in Pgs", INFECT. IMMUN., vol. 67, 1999, pages 4376 - 4382, XP000914965
GARAN, J. E. ET AL., INFECT. IMMUN., vol. 58, 1990, pages 3116 - 3121
MAKINO, S. ET AL., MICROB. PATHOG., vol. 25, 1998, pages 101 - 109
IMADA, Y. ET AL., PROC. JPN. PIG. VET. SOC., vol. 34, 1999, pages 12
IMADA, Y. ET AL., INFECT., 1999
IMADA Y. ET AL: "Truncated Surface Protective Antigen (SpaA) of Erysipelothrix rhusiopathiae Serotype la Elicits Protection against challenge with Serotypes 1a and 2b in Pgs", INFECT. IMMUN., vol. 67, 1999, pages 4376 - 4382, XP000914965
GARAN, J. E. ET AL., INFECT. IMMUN., vol. 58, 1990, pages 3116 - 3121
MAKINO, S. ET AL., MICROB. PATHOG., vol. 25, 1998, pages 101 - 109
IMADA, Y. ET AL., PROC. JPN. PIG. VET. SOC., vol. 34, 1999, pages 12
IMADA, Y. ET AL., INFECT., 1999
Attorney, Agent or Firm:
Kawamiya, Osamu (IMP Building 3-7, Shiromi 1-chome, Chuo-k, Osaka-shi Osaka 01, JP)
Download PDF:
Previous Patent: PDE2 CRYSTAL STRUCTURES FOR STRUCTURE BASED DRUG DESIGN
Next Patent: TUMOR ANTIGEN PEPTIDES
Next Patent: TUMOR ANTIGEN PEPTIDES