Title:
PEPTIDE-NUCLEIC ACID COMPLEX
Document Type and Number:
WIPO Patent Application WO/2020/095985
Kind Code:
A1
Abstract:
Provided is a method for producing a peptide-nucleic acid complex that contains a peptide and nucleic acid encoding the peptide. The method comprises a step of preparing a nucleic acid containing a first coding sequence that encodes a peptide, a second coding sequence that encodes a transpeptidase, and a third coding sequence that encodes a motif for recognizing the transpeptidase, the nucleic acid including a transpeptidase N-terminal substrate motif added thereto; a step of synthesizing, from the transpeptidase N-terminal substrate motif-added nucleic acid and using a cell-free protein synthesis system, a chimeric protein that contains a domain of the peptide, a domain of the transpeptidase, and the motif for recognizing the transpeptidase; and a step of forming the peptide-nucleic acid complex by means of the transpeptidase domain.
Inventors:
UENO SHINGO (JP)
ICHIKI TAKANORI (JP)
ICHIKI TAKANORI (JP)
Application Number:
PCT/JP2019/043640
Publication Date:
May 14, 2020
Filing Date:
November 07, 2019
Export Citation:
Assignee:
KAWASAKI INST OF INDUSTRIAL PROMOTION (JP)
International Classes:
C07K17/00; C07K19/00; C12N11/00; C12N15/09; C12N15/54; C12N15/62; C12P21/00; C12Q1/686; C12Q1/6876; C40B40/08; C12N9/10
Domestic Patent References:
| WO2005001086A1 | 2005-01-06 | |||
| WO2018168999A1 | 2018-09-20 |
Foreign References:
| JP2016523978A | 2016-08-12 | |||
| JP2016519118A | 2016-06-30 | |||
| JP2018015013A | 2018-02-01 |
Other References:
PRITZ, S. ET AL.: "Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation", J. ORG. CHEM., vol. 72, 2007, pages 3909 - 3912, XP002459582, ISSN: 0022-3263, DOI: 10.1021/jo062331l
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
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