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Patent Searching and Data


Title:
PHOSPHOROUS PROTECTING GROUPS AND METHODS OF PREPARATION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/168461
Kind Code:
A3
Abstract:
Aspects of the present disclosure include compositions that make use of phosphorus and/or nucleobase protecting groups which find use in the synthesis of long polynucleotides. Phosphorus protecting groups are provided that help increase the stepwise coupling yield and/or phosphorous protecting groups that can be removed during the oxidation step. Amidine nucleobase protecting groups are provided that find use in the subject compositions and methods which provides for e.g., increased resistance to depurination during polynucleotide synthesis. In some instances, the methods and compositions disclosed herein utilize a combination of the phosphorus and amidine nucleobase protecting groups in the synthesis of polynucleotides having a sequence of 200 or more monomeric units in length. Also provided are methods for synthesizing a polynucleotide (e.g., a DNA) using one or more compounds disclosed herein.

Inventors:
DELLINGER DOUGLAS J (US)
MONFREGOLA LUCA (US)
CARUTHERS MARVIN (US)
ROY MITHUM (US)
Application Number:
PCT/US2015/028624
Publication Date:
December 23, 2015
Filing Date:
April 30, 2015
Export Citation:
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Assignee:
AGILENT TECHNOLOGIES INC (US)
International Classes:
C07H19/16
Foreign References:
US6274725B12001-08-14
US20060178507A12006-08-10
Other References:
DATABASE PubChem. [o] 14 March 2007 (2007-03-14), Database accession no. 23213079.
DATABASE PubChem. 14 March 2007 (2007-03-14), XP055245957, Database accession no. 23300532.
See also references of EP 3137478A4
Attorney, Agent or Firm:
SCHERER, David C. (MS: 1A-PBSanta Clara, California, US)
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