Title:
POLYEPTIDE CROSSLINKED PROTEIN MOLECULAR IMPRINTING POLYMER, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/172973
Kind Code:
A1
Abstract:
A polyeptide crosslinked protein molecular imprinting polymer, a preparation method therefor, and an application thereof. The preparation method comprises the following steps: 1) mixing and dissolving a main monomer, a functional monomer, a polypeptide crosslinking agent, and a template protein in a water-soluble solvent to obtain a mixed solution; 2) adding an initiator to the mixed solution, and performing polymerization in the presence of a polypeptide crosslinker in a helix conformation; and 3) performing template eluting in the presence of a polypeptide crosslinker in a coil conformation to obtain the a polypeptide crosslinked protein molecular imprinting polymer, wherein the polypeptide crosslinker is a polypeptide having polymerizable double bonds at both ends of an amino acid sequence and being able to undergo helix-coil conformational transition. The polypeptide crosslinked protein molecular imprinting polymer can completely remove the template protein under a mild condition, and can improve the imprinting effect of the protein molecular imprinting polymer.
Inventors:
ZHANG YONGJUN (CN)
XU RONG (CN)
GUAN YING (CN)
XU RONG (CN)
GUAN YING (CN)
Application Number:
PCT/CN2019/083343
Publication Date:
September 03, 2020
Filing Date:
April 19, 2019
Export Citation:
Assignee:
UNIV NANKAI (CN)
International Classes:
C08F289/00; B01J20/26; B01J20/30; C08F220/54; C08J9/26
Foreign References:
| CN104086696A | 2014-10-08 | |||
| CN106414574A | 2017-02-15 | |||
| CN104403043A | 2015-03-11 | |||
| JP2000500777A | 2000-01-25 | |||
| US20060240435A1 | 2006-10-26 | |||
| CN201910147938A | 2019-02-28 |
Other References:
CHEN, ZHIYONG ET AL.: "Protein-responsive Imprinted Polymers with Specific Shrinking and Rebinding", JOURNAL OF MOLECULAR RECOGNITION, vol. 21, no. 1, 28 February 2008 (2008-02-28), XP55729160, DOI: 20191127100317Y
KAZUYA MATSUMOTO ET AL.: "Conformationally Regulated Molecular Binding and Release of Molecularly Imprinted Polypeptide Hydrogels That Undergo Helix−Coil Transition", MACROMOLECULES, vol. 50, no. 5, 23 February 2017 (2017-02-23), XP55729161, DOI: 20191127100638A
Z. CHEN ET AL., JOURNAL OF MOLECULAR RECOGNITION, vol. 21, no. 1, 2008, pages 71 - 77
CULVER, H. R.PEPPAS, N. A., CHEMISTRY OF MATERIALS, vol. 29, no. 14, 2017, pages 5753 - 5761
Z. HUA ET AL., LANGMUIR, vol. 24, 2008, pages 5773 - 5780
See also references of EP 3722344A4
KAZUYA MATSUMOTO ET AL.: "Conformationally Regulated Molecular Binding and Release of Molecularly Imprinted Polypeptide Hydrogels That Undergo Helix−Coil Transition", MACROMOLECULES, vol. 50, no. 5, 23 February 2017 (2017-02-23), XP55729161, DOI: 20191127100638A
Z. CHEN ET AL., JOURNAL OF MOLECULAR RECOGNITION, vol. 21, no. 1, 2008, pages 71 - 77
CULVER, H. R.PEPPAS, N. A., CHEMISTRY OF MATERIALS, vol. 29, no. 14, 2017, pages 5753 - 5761
Z. HUA ET AL., LANGMUIR, vol. 24, 2008, pages 5773 - 5780
See also references of EP 3722344A4
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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