Title:
POLYPEPTIDE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/136521
Kind Code:
A1
Abstract:
Disclosed is a polypeptide. The polypeptide has a modification at the N-terminus compared with polypeptides having the following amino acid sequences: (1) a polypeptide having the amino acid sequences represented by SEQ ID NOs: 1 to 6; or (2) a polypeptide having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% identity to (1); or (3) a polypeptide with substitution, deletion and/or addition of one or more amino acids compared with (1), wherein the modification comprises at least one modification selected from addition, methylation, formylation, carbamylation, succinylation, cyclization, lysine propionylation, hexadecanoylation, myristylation, and acetylation of N-terminal amino acids. The polypeptide not only maintains the activity of BefA proteins to promote the proliferation of beta-cells, but also has the characteristics of higher stability and difficult degradation. Furthermore, the polypeptide also has the potential to lower glucose, promote weight loss, and protect the liver.
Inventors:
LI LIJIA (CN)
GU XIANGDI (CN)
WU DU (CN)
GUO LINFENG (CN)
LI XIAOPING (CN)
HU YULONG (CN)
LI YUSHENG (CN)
LI YU (CN)
XU LINGHUA (CN)
LI JING (CN)
CHEN XIAOFENG (CN)
LI WENJIA (CN)
GU XIANGDI (CN)
WU DU (CN)
GUO LINFENG (CN)
LI XIAOPING (CN)
HU YULONG (CN)
LI YUSHENG (CN)
LI YU (CN)
XU LINGHUA (CN)
LI JING (CN)
CHEN XIAOFENG (CN)
LI WENJIA (CN)
Application Number:
PCT/CN2020/142229
Publication Date:
July 08, 2021
Filing Date:
December 31, 2020
Export Citation:
Assignee:
DONGGUAN HEC BIOPHARMACEUTICAL R&D CO LTD (CN)
International Classes:
C07K14/195; A61K38/00; C07K1/06; C07K19/00; C12N15/70
Foreign References:
| US20170292115A1 | 2017-10-12 | |||
| US20170292115A1 | 2017-10-12 | |||
| CN101500592A | 2009-08-05 | |||
| CN107245494A | 2017-10-13 | |||
| CN105669833A | 2016-06-15 | |||
| CN105753945A | 2016-07-13 |
Other References:
DATABASE PROTEIN 23 February 2015 (2015-02-23), ANONYMOUS: "lipoprotein [Aeromonas hydrophila]", XP055825820, retrieved from NCBI Database accession no. WP_043124314
HAMPTON HILL JENNIFER, FRANZOSA ERIC A, HUTTENHOWER CURTIS, GUILLEMIN KAREN: "A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development", ELIFE, 13 December 2016 (2016-12-13), XP055825824, Retrieved from the Internet DOI: 10.7554/eLife.20145
HU MEILIN, DONG HUI;GONG JING: "Regeneration of Islet β cells: A New Strategy for Diabetes", CHINESE JOURNAL OF DIABETES, vol. 24, no. 12, 1 December 2016 (2016-12-01), pages 1148 - 1152, XP055827924, ISSN: 1006-6187, DOI: 10.3969/j.issn.1006-6187.2016.12.021
HAMPTON HILL JENNIFER, FRANZOSA ERIC A, HUTTENHOWER CURTIS, GUILLEMIN KAREN: "A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development", ELIFE, 13 December 2016 (2016-12-13), XP055825824, Retrieved from the Internet
HU MEILIN, DONG HUI;GONG JING: "Regeneration of Islet β cells: A New Strategy for Diabetes", CHINESE JOURNAL OF DIABETES, vol. 24, no. 12, 1 December 2016 (2016-12-01), pages 1148 - 1152, XP055827924, ISSN: 1006-6187, DOI: 10.3969/j.issn.1006-6187.2016.12.021
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