Title:
POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2005/118815
Kind Code:
A1
Abstract:
A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
Inventors:
SATO YOSHIMI (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
Application Number:
PCT/JP2005/008711
Publication Date:
December 15, 2005
Filing Date:
May 12, 2005
Export Citation:
Assignee:
TAKARA BIO INC (JP)
SATO YOSHIMI (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
SATO YOSHIMI (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
International Classes:
C07K16/40; C12N9/10; C12N9/12; C12N15/54; C12Q1/68; (IPC1-7): C12N15/54; C07K16/40; C12N9/10; C12Q1/68
Domestic Patent References:
| WO2003054139A2 | 2003-07-03 |
Foreign References:
| JPH07298879A | 1995-11-14 | |||
| JPH1042872A | 1998-02-17 | |||
| JP2002253265A | 2002-09-10 | |||
| JP2004500052A | 2004-01-08 |
Other References:
SOUTHWORTH M.W. ET AL: "Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp.9 degrees N-7 and mutations affecting 3'-5' exonuclease activity.", PROC.NATL.ACAD.SCI.USA., vol. 93, no. 11, 1996, pages 5281 - 5285, XP002171947
KUNKEL, J. BIOL. CHEM., vol. 260, no. 9, 10 May 1985 (1985-05-10), pages 5787 - 96
CLINE ET AL., NUCLEIC ACIDS RES., vol. 24, no. 18, 15 September 1996 (1996-09-15), pages 3546 - 51
"DNA polymerase from Escherichia coli", HARPER & ROW, pages: 263 - 276
See also references of EP 1757698A4
KUNKEL, J. BIOL. CHEM., vol. 260, no. 9, 10 May 1985 (1985-05-10), pages 5787 - 96
CLINE ET AL., NUCLEIC ACIDS RES., vol. 24, no. 18, 15 September 1996 (1996-09-15), pages 3546 - 51
"DNA polymerase from Escherichia coli", HARPER & ROW, pages: 263 - 276
See also references of EP 1757698A4
Attorney, Agent or Firm:
Kawamiya, Osamu (IMP Building 3-7, Shiromi 1-chome, Chuo-ku, Osaka-sh, Osaka 01, JP)
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