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Title:
POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2005/118815
Kind Code:
A1
Abstract:
A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.

Inventors:
SATO YOSHIMI (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
Application Number:
PCT/JP2005/008711
Publication Date:
December 15, 2005
Filing Date:
May 12, 2005
Export Citation:
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Assignee:
TAKARA BIO INC (JP)
SATO YOSHIMI (JP)
NISHIWAKI KAZUE (JP)
SHIMADA NANA (JP)
HOKAZONO SHIGEKAZU (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
KATO IKUNOSHIN (JP)
International Classes:
C07K16/40; C12N9/10; C12N9/12; C12N15/54; C12Q1/68; (IPC1-7): C12N15/54; C07K16/40; C12N9/10; C12Q1/68
Domestic Patent References:
WO2003054139A22003-07-03
Foreign References:
JPH07298879A1995-11-14
JPH1042872A1998-02-17
JP2002253265A2002-09-10
JP2004500052A2004-01-08
Other References:
SOUTHWORTH M.W. ET AL: "Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp.9 degrees N-7 and mutations affecting 3'-5' exonuclease activity.", PROC.NATL.ACAD.SCI.USA., vol. 93, no. 11, 1996, pages 5281 - 5285, XP002171947
See also references of EP 1757698A4
KUNKEL, J. BIOL. CHEM., vol. 260, no. 9, 10 May 1985 (1985-05-10), pages 5787 - 96
CLINE ET AL., NUCLEIC ACIDS RES., vol. 24, no. 18, 15 September 1996 (1996-09-15), pages 3546 - 51
"DNA polymerase from Escherichia coli", HARPER & ROW, pages: 263 - 276
Attorney, Agent or Firm:
Kawamiya, Osamu (IMP Building 3-7, Shiromi 1-chome, Chuo-ku, Osaka-sh, Osaka 01, JP)
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