Title:
A PRECESS FOR PREPARATION OF TIMOSAPONIN B II
Document Type and Number:
WIPO Patent Application WO/2005/105824
Kind Code:
A1
Abstract:
A precess for preparation of timosaponin B II, which uses Chinese traditional drug Anemarrhena Rhizome or newly collected rhizoma or fibrous root of Anemarrhena Asphodeloides Bge. as raw material, and comprises isolation of tiBosaponin B II by one or more steps selected from solvent extraction, resin adsorption, polyamide chromatography, inversed phase column chromatography, column chromatography on Sephadex LH-20 and so on in combination with conventional drying method such as vacuum drying, freeze drying, spray drying and so on. Timosaponin B II obtained by the present process is of over 90% purity, and the process is simple and applicable and suitable for industrial production.
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Inventors:
MA BAIPING (CN)
CHEN HAO (CN)
XIONG CHENGQI (CN)
KANG LIPING (CN)
ZHANG JIE (CN)
CHEN HAO (CN)
XIONG CHENGQI (CN)
KANG LIPING (CN)
ZHANG JIE (CN)
Application Number:
PCT/CN2005/000554
Publication Date:
November 10, 2005
Filing Date:
April 21, 2005
Export Citation:
Assignee:
INST RADIATION MED AMMS PLA (CN)
MA BAIPING (CN)
CHEN HAO (CN)
XIONG CHENGQI (CN)
KANG LIPING (CN)
ZHANG JIE (CN)
MA BAIPING (CN)
CHEN HAO (CN)
XIONG CHENGQI (CN)
KANG LIPING (CN)
ZHANG JIE (CN)
International Classes:
C07J53/00; C07J71/00; (IPC1-7): C07J71/00
Foreign References:
JP2000204043A | 2000-07-25 | |||
JPH0454194A | 1992-02-21 |
Other References:
BAIPING MA. ET AL: "Studies on the furostanol saponins from Anamarrhena asphodeloides Bunge.", vol. 31, no. 4, 1996, pages 271 - 277, XP008054989
ZHANG J. ET AL: "Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood.", CLINICA CHIMICA ACTA., vol. 289, no. 1-2, 1999, pages 79 - 88, XP002409220
NOBORU NAKASHIMA ET AL: "Isolation of pseudoprototimosaponin AIII from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocin-induced diabetic mice.", J NAT PROD., vol. 56, no. 3, March 1993 (1993-03-01), pages 345 - 350, XP003009420
See also references of EP 1752464A4
ZHANG J. ET AL: "Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood.", CLINICA CHIMICA ACTA., vol. 289, no. 1-2, 1999, pages 79 - 88, XP002409220
NOBORU NAKASHIMA ET AL: "Isolation of pseudoprototimosaponin AIII from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocin-induced diabetic mice.", J NAT PROD., vol. 56, no. 3, March 1993 (1993-03-01), pages 345 - 350, XP003009420
See also references of EP 1752464A4
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (Vantone New World Plaza Fuchengmenwai Street, Beijing 7, CN)
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