Title:
PREPARATION METHOD FOR MOMORDIN IB
Document Type and Number:
WIPO Patent Application WO/2020/221113
Kind Code:
A1
Abstract:
Provided by the present invention is a preparation method for momordin Ib, comprising: first, mixing a plant containing a triterpene saponin derivative having the structural unit shown in formula (I) or a plant extract containing a triterpene saponin derivative having the structural unit shown in formula (I) with acid for reaction to obtain an extract, then using an alkali to adjust the pH of the extract obtained in step 1) to 4.0-7.5, and then separating same by means of column chromatography to obtain a momordin Ib-containing sample; and finally, recrystallizing the obtained momordin Ib-containing sample to obtain a pure momordin Ib product. According to the present invention, a plant or plant extract rich in a triterpene saponin derivative having the structural unit shown in formula (I) is used as a raw material for preparing momordin Ib. As a result, it is found that the described method features simple separation and purification operations, and a large amount of momordin Ib may be obtained. In addition, the solvents eluted and recrystallized in each step may be recycled multiple times, being suitable for industrial production.
Inventors:
ZHANG YUMEI (CN)
LI JIA (CN)
LI JINGYA (CN)
ZANG YI (CN)
SUN YILI (CN)
LI JIA (CN)
LI JINGYA (CN)
ZANG YI (CN)
SUN YILI (CN)
Application Number:
PCT/CN2020/086541
Publication Date:
November 05, 2020
Filing Date:
April 24, 2020
Export Citation:
Assignee:
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCIENCES (CN)
SHANGHAI INSTITUTE OF MAT AND MEDICA (CN)
SHANGHAI INSTITUTE OF MAT AND MEDICA (CN)
International Classes:
C07J63/00; C07J9/00
Foreign References:
| CN1355172A | 2002-06-26 | |||
| CN104910240A | 2015-09-16 | |||
| JPH1112178A | 1999-01-19 |
Other References:
XU HUAN ,LI YUANYUAN , WANG FEI ,ZHANG LI ,SONG BEI ,YANG XINJIE ,SONG XIAOMEI: "Study on the thermal conversion mechanism of saponins in Panacis Majoris Rhizoma under acidic condition", NORTHWEST PHARMACEUTICAL JOURNAL, vol. 32, no. 5, 10 September 2017 (2017-09-10), pages 559 - 562, XP055750720
JONGWON CHOI , KYUNG-TAE LEE, HYUN-JU JUNG, HEE-SUN PARK , HEE-JUHN PARK: "Anti-Rheumatoid Arthritis Effect of the Kochia scoparia Fruits and Activity Comparison of Momordin Ic, its Prosapogenin and Sapogenin", ARCHIVES OF PHARMACAL RESEARCH, vol. 25, no. 3, 30 June 2002 (2002-06-30), pages 336 - 342, XP055750728, ISSN: 0253-6269
LU XIANG-HONG, XU XIANG-DONG,FU HONG-WEI,CHEN BO, TIAN JING-KUI, ZHANG LIN: "Study on Chemical Constituents of Kochia scoparia", CHINESE PHARMACEUTICAL JOURNAL, vol. 47, no. 5, 8 March 2012 (2012-03-08), pages 338 - 342, XP055750746
JONGWON CHOI , KYUNG-TAE LEE, HYUN-JU JUNG, HEE-SUN PARK , HEE-JUHN PARK: "Anti-Rheumatoid Arthritis Effect of the Kochia scoparia Fruits and Activity Comparison of Momordin Ic, its Prosapogenin and Sapogenin", ARCHIVES OF PHARMACAL RESEARCH, vol. 25, no. 3, 30 June 2002 (2002-06-30), pages 336 - 342, XP055750728, ISSN: 0253-6269
LU XIANG-HONG, XU XIANG-DONG,FU HONG-WEI,CHEN BO, TIAN JING-KUI, ZHANG LIN: "Study on Chemical Constituents of Kochia scoparia", CHINESE PHARMACEUTICAL JOURNAL, vol. 47, no. 5, 8 March 2012 (2012-03-08), pages 338 - 342, XP055750746
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
Download PDF:
Previous Patent: AIR-SUCTION GARLIC ORIENTING AND SEEDING DEVICE
Next Patent: METHOD AND DEVICE FOR DISPLAYING INFORMATION
Next Patent: METHOD AND DEVICE FOR DISPLAYING INFORMATION