Title:
PRIMARY BREAST EPITHELIAL CELL CULTURE MEDIUM, CULTURE METHOD, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/088119
Kind Code:
A1
Abstract:
Provided is a culture medium containing amphiregulin for culturing primary breast epithelial cells and a culture method involving using the medium. In the culture method, primary cells are cultured on a culture vessel coated with an extracellular matrix gel by using the culture medium, and the primary cells grow on the culture vessel, which has been coated with the extracellular matrix gel, and proliferate rapidly under the combined action of nutrient factors and an extracellular matrix contained in the primary cell culture medium. The cell model obtained by the primary cell culture medium and the primary cell culture method can be used for the evaluating the efficacy of and screening drugs.
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Inventors:
LIU QINGSONG (CN)
LIU FEIYANG (CN)
MEI HUSHENG (CN)
WANG WENCHAO (CN)
ZHAO MING (CN)
JIANG ZONGRU (CN)
REN TAO (CN)
WANG LI (CN)
LIU FEIYANG (CN)
MEI HUSHENG (CN)
WANG WENCHAO (CN)
ZHAO MING (CN)
JIANG ZONGRU (CN)
REN TAO (CN)
WANG LI (CN)
Application Number:
PCT/CN2019/119116
Publication Date:
May 14, 2021
Filing Date:
November 18, 2019
Export Citation:
Assignee:
PRECEDO PHARMACEUTICALS CO LTD (CN)
International Classes:
C12N5/095; C12N5/00; C12N5/071
Domestic Patent References:
| WO2016083612A1 | 2016-06-02 | |||
| WO2016083613A2 | 2016-06-02 | |||
| WO2016180421A1 | 2016-11-17 |
Foreign References:
| US5830995A | 1998-11-03 |
Other References:
MUKHOPADHYAY CHANDRANI, ZHAO XIANGSHAN, MARONI DULCE, BAND VIMLA, NARAMURA MAYUMI: "Distinct Effects of EGFR Ligands on Human Mammary Epithelial Cell Differentiation", PLOS ONE, vol. 8, no. 10, 4 October 2013 (2013-10-04), pages e75907, XP055810064, DOI: 10.1371/journal.pone.0075907
ADAM A. FRIEDMAN ET AL., NAT REV CANCER, vol. 15, no. 12, 2015, pages 747 - 56
LIU ET AL., AM J PATHOL, vol. 180, 2012, pages 599 - 607
HANS CLEVERS ET AL., CELL, vol. 172, no. 1-2, 2018, pages 373 - 386
LIU ET AL., AM J PATHOL, vol. 183, no. 6, 2013, pages 1862 - 1870
LIU ET AL., CELL DEATH DIS., vol. 9, no. 7, 2018, pages 750
NICK BARKER, NAT CELL BIOL, vol. 18, no. 3, 2016, pages 246 - 54
LIU ET AL., NAT PROTOC., vol. 12, no. 2, 2017, pages 439 - 451
GREENWOOD ET AL., ENVIRON MOL MUTAGEN, vol. 43, no. 1, 2004, pages 36 - 44
YU ET AL., SCIENCE, vol. 345, no. 6193, 2014, pages 216 - 220
See also references of EP 4056685A4
ADAM A. FRIEDMAN ET AL., NAT REV CANCER, vol. 15, no. 12, 2015, pages 747 - 56
LIU ET AL., AM J PATHOL, vol. 180, 2012, pages 599 - 607
HANS CLEVERS ET AL., CELL, vol. 172, no. 1-2, 2018, pages 373 - 386
LIU ET AL., AM J PATHOL, vol. 183, no. 6, 2013, pages 1862 - 1870
LIU ET AL., CELL DEATH DIS., vol. 9, no. 7, 2018, pages 750
NICK BARKER, NAT CELL BIOL, vol. 18, no. 3, 2016, pages 246 - 54
LIU ET AL., NAT PROTOC., vol. 12, no. 2, 2017, pages 439 - 451
GREENWOOD ET AL., ENVIRON MOL MUTAGEN, vol. 43, no. 1, 2004, pages 36 - 44
YU ET AL., SCIENCE, vol. 345, no. 6193, 2014, pages 216 - 220
See also references of EP 4056685A4
Attorney, Agent or Firm:
SHANGCHENG & PARTNERS (CN)
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