Title:
PROCESS FOR PRODUCING PROTEINS
Document Type and Number:
WIPO Patent Application WO/1996/012816
Kind Code:
A1
Abstract:
A process for producing a protein by growing host cells capable of expressing the target protein by feeding culture, wherein the specific growth rate of the host cells is switched over from the initial value to a given value by continuously varying the rate of addition of the substrate that controls host cell growth to the culture medium. The mode of addition rate variation according to the invention can realize optimum patterns of specific growth rate and specific production rate aiming at optimizing the feeding culture system. As a result, it is possible to conduct high-density culture of host cells by feeding culture and to produce desired proteins efficiently in a short time.
Inventors:
KOBAYASHI KAORU (JP)
TOMOMITSU KENJI (JP)
KUWAE SHINOBU (JP)
OHYA TOMOSHI (JP)
OHDA TOYOO (JP)
OHMURA TAKAO (JP)
TOMOMITSU KENJI (JP)
KUWAE SHINOBU (JP)
OHYA TOMOSHI (JP)
OHDA TOYOO (JP)
OHMURA TAKAO (JP)
Application Number:
PCT/JP1995/002186
Publication Date:
May 02, 1996
Filing Date:
October 25, 1995
Export Citation:
Assignee:
GREEN CROSS CORP (JP)
KOBAYASHI KAORU (JP)
TOMOMITSU KENJI (JP)
KUWAE SHINOBU (JP)
OHYA TOMOSHI (JP)
OHDA TOYOO (JP)
OHMURA TAKAO (JP)
KOBAYASHI KAORU (JP)
TOMOMITSU KENJI (JP)
KUWAE SHINOBU (JP)
OHYA TOMOSHI (JP)
OHDA TOYOO (JP)
OHMURA TAKAO (JP)
International Classes:
C07K14/765; C12N1/19; C12N1/38; C12N15/00; C12N15/12; C12N15/18; C12P21/00; (IPC1-7): C12P21/00; C12N15/12; C12N1/19
Foreign References:
| JPH0383595A | 1991-04-09 |
Other References:
SHIOYA S., "Optimization and Control in Fed-Batch Cultures", HAKKOKOGAKUKAISHI, Vol. 70, No. 5, (1992), p. 395-404.
See also references of EP 0736605A4
See also references of EP 0736605A4
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