Title:
PRODUCTION METHOD FOR GLYCEROL PHOSPHATE
Document Type and Number:
WIPO Patent Application WO/2014/129492
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for the efficient phosphorylation of glycerol. The problem is solved by reacting glycerol with either a kinase that includes the active center expressed by sequence (1) and exhibits a catalytic activity with respect to the phosphorylation of glycerol, or a kinase that includes the amino acid sequence represented by SEQ ID NO: 2 and exhibits a catalytic activity with respect to the phosphorylation of glycerol, in the presence of a phosphate group donor.
Inventors:
IWATA HIDEYUKI (JP)
TANAKA HISAE (JP)
TANAKA HISAE (JP)
Application Number:
PCT/JP2014/053888
Publication Date:
August 28, 2014
Filing Date:
February 19, 2014
Export Citation:
Assignee:
THERMOSTABLE ENZYME LAB CO LTD (JP)
International Classes:
C12P9/00; C12N15/09
Foreign References:
| EP0462833A2 | 1991-12-27 |
Other References:
HERK TV ET AL.: "Regioselective Phosphorylation of Carbohydrates and Various Alcohols by Bacterial Acid Phosphatases; Probing the Substrate Specificity of the Enzyme from Shigella flexneri", ADV SYNTH CATAL., vol. 347, no. 7, 1 January 2005 (2005-01-01), pages 1155 - 1162, XP002416329, DOI: 10.1002/ADSC.200505072
BABICH L ET AL.: "Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale", CHEM EUR J., vol. 18, no. 21, 21 May 2012 (2012-05-21), pages 6604 - 6609, XP055283547, DOI: 10.1002/CHEM.201200101
DATABASE UNIPROT [online] 13 June 2012 (2012-06-13), IYER PR ET AL., XP055283618, retrieved from IBIS Database accession no. D5QGJ0
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), SALZBERG SL ET AL.: "SUBNAME: FULL=PHOSPHATASE", XP055293032, retrieved from IBIS Database accession no. B2SUV7
DATABASE UNIPROT [online] 28 November 2012 (2012-11-28), BROWN PJB ET AL.: "Definition: SubName: Full=Major phosphate- irrepressible acid phosphatase", XP055283561, retrieved from IBIS Database accession no. F4R2A1
POND JL ET AL.: "Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC+ product, from Zymomonas mobilis", J BACTERIOL., vol. 171, no. 2, 1 February 1989 (1989-02-01), pages 767 - 774, XP055283551
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), NAKAZAWA H ET AL.: "Definition: SubName: Full=Acid phosphatase", XP055283568, retrieved from IBIS Database accession no. C4XPK0
DATABASE UNIPROT [online] 1 March 2013 (2013-03-01), PRUST C ET AL.: "Definition: SubName: Full=Nonspecific acid phosphatase", XP055283573, retrieved from IBIS Database accession no. Q5FQI1
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), LUCAS S ET AL.: "SUBNAME: FULL=ACID PHOSPHATASE", XP055293037, retrieved from IBIS Database accession no. G0B7H8
DATABASE UNIPROT [online] 28 November 2012 (2012-11-28), LUCAS S ET AL.: "Definition: SubName: Full=Acid phosphatase", XP055293043, retrieved from IBIS Database accession no. E8XYI1
UCHIYA K ET AL.: "Identification and characterization of phoN-Sf, a gene on the large plasmid of Shigella flexneri 2a encoding a nonspecific phosphatase", J BACTERIOL., vol. 178, no. 8, 1 August 1996 (1996-08-01), pages 4548 - 4554, XP055283622
CELL MOL LIFE SCI., vol. 54, no. 8, August 1998 (1998-08-01), pages 833 - 50
PROTEIN ENG., vol. 11, no. 12, December 1998 (1998-12-01), pages 1219 - 27
THE EMBO JOURNAL, vol. 19, no. 11, pages 2412 - 2423
See also references of EP 2960337A4
BABICH L ET AL.: "Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale", CHEM EUR J., vol. 18, no. 21, 21 May 2012 (2012-05-21), pages 6604 - 6609, XP055283547, DOI: 10.1002/CHEM.201200101
DATABASE UNIPROT [online] 13 June 2012 (2012-06-13), IYER PR ET AL., XP055283618, retrieved from IBIS Database accession no. D5QGJ0
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), SALZBERG SL ET AL.: "SUBNAME: FULL=PHOSPHATASE", XP055293032, retrieved from IBIS Database accession no. B2SUV7
DATABASE UNIPROT [online] 28 November 2012 (2012-11-28), BROWN PJB ET AL.: "Definition: SubName: Full=Major phosphate- irrepressible acid phosphatase", XP055283561, retrieved from IBIS Database accession no. F4R2A1
POND JL ET AL.: "Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC+ product, from Zymomonas mobilis", J BACTERIOL., vol. 171, no. 2, 1 February 1989 (1989-02-01), pages 767 - 774, XP055283551
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), NAKAZAWA H ET AL.: "Definition: SubName: Full=Acid phosphatase", XP055283568, retrieved from IBIS Database accession no. C4XPK0
DATABASE UNIPROT [online] 1 March 2013 (2013-03-01), PRUST C ET AL.: "Definition: SubName: Full=Nonspecific acid phosphatase", XP055283573, retrieved from IBIS Database accession no. Q5FQI1
DATABASE UNIPROT [online] 6 February 2013 (2013-02-06), LUCAS S ET AL.: "SUBNAME: FULL=ACID PHOSPHATASE", XP055293037, retrieved from IBIS Database accession no. G0B7H8
DATABASE UNIPROT [online] 28 November 2012 (2012-11-28), LUCAS S ET AL.: "Definition: SubName: Full=Acid phosphatase", XP055293043, retrieved from IBIS Database accession no. E8XYI1
UCHIYA K ET AL.: "Identification and characterization of phoN-Sf, a gene on the large plasmid of Shigella flexneri 2a encoding a nonspecific phosphatase", J BACTERIOL., vol. 178, no. 8, 1 August 1996 (1996-08-01), pages 4548 - 4554, XP055283622
CELL MOL LIFE SCI., vol. 54, no. 8, August 1998 (1998-08-01), pages 833 - 50
PROTEIN ENG., vol. 11, no. 12, December 1998 (1998-12-01), pages 1219 - 27
THE EMBO JOURNAL, vol. 19, no. 11, pages 2412 - 2423
See also references of EP 2960337A4
Attorney, Agent or Firm:
Yamada, Iichiro et al. (JP)
Yamada 威 1 郎 (JP)
Yamada 威 1 郎 (JP)
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