Title:
A RECOMBINANT STRAIN PRODUCING MALTOGENIC AMYLASE AND ITS APPLICATION IN BAKED FOOD
Document Type and Number:
WIPO Patent Application WO/2022/042005
Kind Code:
A1
Abstract:
Provided is a recombinant strain producing maltogenic amylase and its application in baked foods, belonging to the technical fields of genetic engineering, enzyme engineering and food engineering. The enzyme activity of recombinant maltogenic amylase BLMA is as high as 3235.0 U/mg at 60℃ and pH 6.5, with good pH stability and wide range of temperature. Through the use of error-prone PCR, gene-directed mutagenesis technologies, mutant with better enzyme activity and better optimum temperature was obtained, the enzyme activity of the mutant BLMA1 was increased to 2.16 times, and the optimum temperature was increased to 65℃. By applying BLMA and BLMA1 to baked foods, both of them could effectively delay the aging of baked foods, and improve the shelf life of foods.
Inventors:
ZHANG RONGZHEN (CN)
XU YAN (CN)
RUAN YINGQI (CN)
XU YAN (CN)
RUAN YINGQI (CN)
Application Number:
PCT/CN2021/103084
Publication Date:
March 03, 2022
Filing Date:
June 29, 2021
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
A21D8/04; C12N9/28
Domestic Patent References:
| WO2019193102A1 | 2019-10-10 |
Foreign References:
| CN111132553A | 2020-05-08 | |||
| CN111944785A | 2020-11-17 | |||
| CN109982576A | 2019-07-05 | |||
| CN111148841A | 2020-05-12 |
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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