Title:
SCHISTOSOMA HAEMATOBIUM RECOMBINANT FUSION PROTEIN SHSAP AND APPLICATION THEREOF IN IMMUNODIAGNOSIS OF SCHISTOSOMIASIS
Document Type and Number:
WIPO Patent Application WO/2021/057003
Kind Code:
A1
Abstract:
A Schistosoma haematobium recombinant fusion protein ShSAP and an application thereof in the immunodiagnosis of schistosomiasis. The Schistosoma haematobium recombinant fusion protein ShSAP can undergo large-scale standardized mass production by means of genetic engineering technology, antigen quality is stable and the repeatability of the results is good. The fusion protein can be used for the immunodiagnosis of both schistosomiasis haematosis and schistosomiasis mansoni, and has extremely high sensitivity. The Schistosoma haematobium recombinant fusion protein ShSAP has a single antigen component, and ShSAP-ELISA has excellent specificity for the immunodiagnosis of schistosomiasis. The fusion protein does not cross-react with the serum of a patient suffering from another parasitic disease, and has broad application prospects.
Inventors:
LIU SHUAI (CN)
CHEN QIJUN (CN)
HOU NAN (CN)
PIAO XIANYU (CN)
CHEN QIJUN (CN)
HOU NAN (CN)
PIAO XIANYU (CN)
Application Number:
PCT/CN2020/082338
Publication Date:
April 01, 2021
Filing Date:
March 31, 2020
Export Citation:
Assignee:
INST PATHOGEN BIOLOGY CAMS (CN)
International Classes:
C12N15/12; C07K19/00; C12N1/21; C12N15/70; G01N33/569
Domestic Patent References:
| WO2015036549A1 | 2015-03-19 |
Foreign References:
| CN110564734A | 2019-12-13 | |||
| CN103102404A | 2013-05-15 |
Other References:
LIU SHUAI, ZHOU XIAOSU, PIAO XIANYU, HOU NAN, SHEN YUJUAN, ZOU YANG, LI SHANSHAN, CAO JIANPING, CHEN QIJUN: "Saposin-like Proteins, a Multigene Family of Schistosoma Species, are Biomarkers for the Immunodiagnosis of Schistosomiasis Japonica", JOURNAL OF INFECTIOUS DISEASES, UNIVERSITY OF CHICAGO PRESS, US, vol. 214, no. 8, 15 October 2016 (2016-10-15), US, pages 1225 - 1234, XP055795222, ISSN: 0022-1899, DOI: 10.1093/infdis/jiw188
DON, T.A. ; BETHONY, J.M. ; LOUKAS, A.: "Saposin-like proteins are expressed in the gastrodermis of Schistosoma mansoni and are immunogenic in natural infections", INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, INTERNATIONAL SOCIETY FOR INFECTIOUS DISEASES, HAMILTON, CA, vol. 12, no. 6, 1 November 2008 (2008-11-01), CA, pages e39 - e47, XP026058865, ISSN: 1201-9712, DOI: 10.1016/j.ijid.2007.10.007
ZHANG, CHUN ET AL.: "Cloning,expression and immunogenicity analysis of saposin-like proteins of Schistosoma japonicum", CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL, vol. 22, no. 3, 31 December 2010 (2010-12-31), pages 228 - 232, XP055795226
DON, T.A. ; BETHONY, J.M. ; LOUKAS, A.: "Saposin-like proteins are expressed in the gastrodermis of Schistosoma mansoni and are immunogenic in natural infections", INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, INTERNATIONAL SOCIETY FOR INFECTIOUS DISEASES, HAMILTON, CA, vol. 12, no. 6, 1 November 2008 (2008-11-01), CA, pages e39 - e47, XP026058865, ISSN: 1201-9712, DOI: 10.1016/j.ijid.2007.10.007
ZHANG, CHUN ET AL.: "Cloning,expression and immunogenicity analysis of saposin-like proteins of Schistosoma japonicum", CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL, vol. 22, no. 3, 31 December 2010 (2010-12-31), pages 228 - 232, XP055795226
Attorney, Agent or Firm:
EIJING HUIJIE INTELLECTUAL PROPERTY FIRM (CN)
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