SELF-PRIMING AND REPLICATING HAIRPIN ADAPTOR FOR CONSTRUCTING NGS LIBRARY, AND METHOD FOR CONSTRUCTING NGS LIBRARY USING SAME

WIPO Patent Application WO/2021/075750

A1

The present invention pertains to: a self-priming and replicating hairpin adaptor for constructing an NGS (next generation sequencing) library; and a method for constructing an NGS library using same. Specifically, the present invention pertains to: a self-priming and replicating hairpin adaptor for constructing an NGS library, the adaptor containing a long single-stranded first oligonucleotide that comprises a nucleotide sequence of SEQ ID NO: 4 and has a hairpin structure, and a short single-stranded second oligonucleotide that comprises a nucleotide sequence of SEQ ID NO: 5; a method for constructing an NGS library using said adaptor; and an NGS library construction kit including said adaptor.

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JUNG CHEUL HEE (KR)
KIM SEO YOUNG (KR)
SHIN JUN Y (KR)

PCT/KR2020/012904

April 22, 2021

September 23, 2020

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UNIV KOREA RES & BUS FOUND (KR)

C12Q1/6855; C12N15/10; C12Q1/6869

WO2017202389A1

2017-11-30

US20120083017A1	2012-04-05
JP2018530536A	2018-10-18
JP2019526267A	2019-09-19

PARK DAECHAN, ELLINGTON ANDREW D, JUNG CHEULHEE: "Selection of self-priming molecular replicators", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 47, no. 5, 18 March 2019 (2019-03-18), pages 2169 - 2176, XP055801123, ISSN: 0305-1048, DOI: 10.1093/nar/gkz044

NAM & NAM (KR)

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