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Title:
SHUTTLE PLASMID REPLICABLE IN CLOSTRIDIUM AND E. COLI AND RECOMBINANT MICROORGANISM PREPARED THEREWITH AND HAVING ENHANCED PENTOSE METABOLISM AND FERMENTATION PERFORMANCE
Document Type and Number:
WIPO Patent Application WO/2019/107940
Kind Code:
A3
Abstract:
The present invention relates to a shuttle plasmid replicable in Clostridium and E. coli, the shuttle plasmid comprising: a nucleotide sequence of the first replication origin allowing replication in E. coli; a nucleotide sequence coding for a replication protein region derived from pUB110 plasmid; and an expression terminator sequence of a gene.

Inventors:
CHOI SUN-HWA (KR)
Application Number:
PCT/KR2018/014875
Publication Date:
July 25, 2019
Filing Date:
November 28, 2018
Export Citation:
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Assignee:
GS CALTEX CORP (KR)
International Classes:
C12N15/70; C12N9/12; C12N9/88; C12N15/65; C12N15/66; C12N15/74
Foreign References:
KR101565726B12015-11-16
Other References:
LEE, S.-H. ET AL.: "A New Shuttle Plasmid That Stably Replicates in Clostridium acetobutylicum", J. MICROBIOL. BIOTECHNOL., vol. 25, no. 10, 2014, pages 1702 - 1708, XP055631290
LEE, 1. Y. ET AL.: "Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production", BIOTECLMOL . J., vol. 4, no. 10, 2009, pages 432 - 1440, XP055016920, doi:10.1002/biot.200900142
DATABASE Nucleotide 18 February 2015 (2015-02-18), "Clostridium acetobutylicum DSM 1731. complete genome", retrieved from NCBI Database accession no. CP002660.1
DATABASE Nucleotide 25 July 2016 (2016-07-25), "Cacelobutylicum adhE. ctfA and ctfB genes", XP055631292, retrieved from NCBI Database accession no. X72831.1
Attorney, Agent or Firm:
DAE-A INTELLECTUAL PROPERTY CONSULTING (KR)
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