Title:
SIALYLTRANSFERASES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/118829
Kind Code:
A3
Abstract:
Provided herein, inter alia, are methods, bacteria, nucleic acids, and polypeptides for producing sialylated oligosaccharides.
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Inventors:
MALLIPEDDI SRIKRISHNAN (US)
HEIDTMAN MATTHEW IAN (US)
MERIGHI MASSIMO (US)
MCCOY JOHN M (US)
HEIDTMAN MATTHEW IAN (US)
MERIGHI MASSIMO (US)
MCCOY JOHN M (US)
Application Number:
PCT/US2018/065656
Publication Date:
July 25, 2019
Filing Date:
December 14, 2018
Export Citation:
Assignee:
GLYCOSYN LLC (US)
International Classes:
C12P19/02; C07H5/06; C12N9/10
Foreign References:
| US20160024543A1 | 2016-01-28 | |||
| US20110014661A1 | 2011-01-20 | |||
| US20140031541A1 | 2014-01-30 |
Other References:
DATABASE UniProtKB [online] 16 October 2013 (2013-10-16), "Putative Alpha-2,3/2,6-sialyltransferase/sialidase", XP055628747, retrieved from UniProt Database accession no. S6F5P9_AVIPA
DATABASE UniProtKB [online] 13 June 2012 (2012-06-13), "Bifunctional alpha-2,3/-2,8-sialyltransferase", XP055628749, retrieved from UniProt Database accession no. I0EP47_HELCO
DATABASE UniProtKB [online] 27 May 2015 (2015-05-27), "Bifunctional alpha-2,3/-2,8-sialyltransferase {ECO:0000313|EMBL:AFH99552.1}", XP055628752, retrieved from UniProt Database accession no. A0A0E0WB72_HELPX
KAKUTA ET AL.: "Crystal structure of Vibrionaceae Photobacterium sp. JT-ISH-224 .alpha.2,6- sialyltransferase in a ternary complex with donor product CMP and acceptor substrate lactose: catalytic mechanism and substrate recognition", GLYCOBIOLOGY, vol. 18, no. 1, 25 October 2007 (2007-10-25), pages 66 - 73, XP055628755
SUGIARTO ET AL.: "A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Directly Sialylating Lewis", ACS CHEMICAL BIOLOGY, vol. 7, no. 7, 14 May 2012 (2012-05-14), pages 1232 - 1240, XP055628758
SCHUR ET AL.: "Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis", GLYCOBIOLOGY, vol. 22, no. 7, 14 April 2012 (2012-04-14), pages 997 - 1006, XP055628761
DAILIDIENE ET AL.: "Helicobacter acinonychis: genetic and rodent infection studies of a Helicobacter pylori-like gastric pathogen of cheetahs and other big cats", JOURNAL OF BACTERIOLOGY, vol. 186, no. 2, December 2003 (2003-12-01), pages 356 - 365, XP055628763
SCHMOLZER ET AL.: "Complete switch from .alpha.-2,3- to .alpha.-2,6-regioselectivity in Pasteurella dagmatis .beta.-D-galactoside sialyltransferase by active-site redesign", CHEMICAL COMMUNICATIONS, vol. 51, no. 15, 21 February 2015 (2015-02-21), pages 3083 - 3086, XP055333647
DATABASE UniProtKB [online] 13 June 2012 (2012-06-13), "Bifunctional alpha-2,3/-2,8-sialyltransferase", XP055628749, retrieved from UniProt Database accession no. I0EP47_HELCO
DATABASE UniProtKB [online] 27 May 2015 (2015-05-27), "Bifunctional alpha-2,3/-2,8-sialyltransferase {ECO:0000313|EMBL:AFH99552.1}", XP055628752, retrieved from UniProt Database accession no. A0A0E0WB72_HELPX
KAKUTA ET AL.: "Crystal structure of Vibrionaceae Photobacterium sp. JT-ISH-224 .alpha.2,6- sialyltransferase in a ternary complex with donor product CMP and acceptor substrate lactose: catalytic mechanism and substrate recognition", GLYCOBIOLOGY, vol. 18, no. 1, 25 October 2007 (2007-10-25), pages 66 - 73, XP055628755
SUGIARTO ET AL.: "A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Directly Sialylating Lewis", ACS CHEMICAL BIOLOGY, vol. 7, no. 7, 14 May 2012 (2012-05-14), pages 1232 - 1240, XP055628758
SCHUR ET AL.: "Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis", GLYCOBIOLOGY, vol. 22, no. 7, 14 April 2012 (2012-04-14), pages 997 - 1006, XP055628761
DAILIDIENE ET AL.: "Helicobacter acinonychis: genetic and rodent infection studies of a Helicobacter pylori-like gastric pathogen of cheetahs and other big cats", JOURNAL OF BACTERIOLOGY, vol. 186, no. 2, December 2003 (2003-12-01), pages 356 - 365, XP055628763
SCHMOLZER ET AL.: "Complete switch from .alpha.-2,3- to .alpha.-2,6-regioselectivity in Pasteurella dagmatis .beta.-D-galactoside sialyltransferase by active-site redesign", CHEMICAL COMMUNICATIONS, vol. 51, no. 15, 21 February 2015 (2015-02-21), pages 3083 - 3086, XP055333647
Attorney, Agent or Firm:
BEATTIE, Ingrid (US)
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