Title:
SPHERICAL CULTURE AND MANUFACTURING METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/138938
Kind Code:
A1
Abstract:
The present invention provides a spherical culture for which quantitative analysis can be easily performed for a region including brain neurons, which is useful as a supply source of neurons for transplantation, and which can be suitably used in an evaluation system for a compound or drug discovery screening for a neurological disorder. Provided are: a spherical culture comprising a first cell layer that includes neural stem cells and/or neuronal precursor cells, and a second cell layer that includes brain neurons, wherein the first cell layer exists on a surface part of the spherical culture; and a manufacturing method for the spherical culture.
Inventors:
INOUE HARUHISA (JP)
IMAMURA KEIKO (JP)
KONDO TAKAYUKI (JP)
DANG NGOC ANH SUONG (JP)
KATO YOSHIKAZU (JP)
IMAMURA KEIKO (JP)
KONDO TAKAYUKI (JP)
DANG NGOC ANH SUONG (JP)
KATO YOSHIKAZU (JP)
Application Number:
PCT/JP2021/048306
Publication Date:
June 30, 2022
Filing Date:
December 24, 2021
Export Citation:
Assignee:
UNIV KYOTO (JP)
SATAKE MULTIMIX CORP (JP)
SATAKE MULTIMIX CORP (JP)
International Classes:
C12N5/0797; A61K35/30; A61L27/38; A61L27/40; A61P25/00; A61P43/00; C12N1/00; C12N5/0793; C12N5/10; C12Q1/06; G01N33/15; G01N33/50
Foreign References:
| JP2018050484A | 2018-04-05 |
Other References:
KATO, YOSHIKAZU: "Development of industrial 3D floating iPS cell differentation induction culture device", KAGAKU-SOCHI = PLANT AND PROCESS, CHOSAKAI, JP, vol. 60, no. 7, 1 January 2018 (2018-01-01), JP , pages 23 - 34, XP009537912, ISSN: 0368-4849
UEKI, MASASHI ET AL.: "P-03-003 Culture of human iPS cells using a cell culture device of vertical stirring motion type (VMF reactor)", LECTURE ABSTRACTS OF THE 18TH CONGRESS OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE; MARCH 21-23, 2019, JAPANESE SOCIETY FOR REGENERATIVE MEDICINE, JP, vol. 18, 1 January 2019 (2019-01-01) - 23 March 2019 (2019-03-23), JP, pages 824, XP009538541
LEE BRIAN, HASHIMURA YAS, JUNG SUNGHOON, ROSELLO FRANCISCO, STARKWEATHER NATHAN, WESSELSCHMIDT ROBIN: "SCALE UP OF PLURIPOTENT STEM CELL CULTIVATION AND DIFFERENTIATION IN A NOVEL SINGLE-USE BIOREACTOR SYSTEM", CYTOTHERAPY, vol. 19, no. 5, 1 January 2017 (2017-01-01), pages 178 - 178, XP055947030
SUONG DANG NGOC ANH, IMAMURA KEIKO, INOUE IKUYO, KABAI RYOTARO, SAKAMOTO SATOKO, OKUMURA TATSUYA, KATO YOSHIKAZU, KONDO TAKAYUKI, : "Induction of inverted morphology in brain organoids by vertical-mixing bioreactors", COMMUNICATIONS BIOLOGY, vol. 4, no. 1, 1 December 2021 (2021-12-01), XP055947031, DOI: 10.1038/s42003-021-02719-5
UEKI, MASASHI ET AL.: "P-03-003 Culture of human iPS cells using a cell culture device of vertical stirring motion type (VMF reactor)", LECTURE ABSTRACTS OF THE 18TH CONGRESS OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE; MARCH 21-23, 2019, JAPANESE SOCIETY FOR REGENERATIVE MEDICINE, JP, vol. 18, 1 January 2019 (2019-01-01) - 23 March 2019 (2019-03-23), JP, pages 824, XP009538541
LEE BRIAN, HASHIMURA YAS, JUNG SUNGHOON, ROSELLO FRANCISCO, STARKWEATHER NATHAN, WESSELSCHMIDT ROBIN: "SCALE UP OF PLURIPOTENT STEM CELL CULTIVATION AND DIFFERENTIATION IN A NOVEL SINGLE-USE BIOREACTOR SYSTEM", CYTOTHERAPY, vol. 19, no. 5, 1 January 2017 (2017-01-01), pages 178 - 178, XP055947030
SUONG DANG NGOC ANH, IMAMURA KEIKO, INOUE IKUYO, KABAI RYOTARO, SAKAMOTO SATOKO, OKUMURA TATSUYA, KATO YOSHIKAZU, KONDO TAKAYUKI, : "Induction of inverted morphology in brain organoids by vertical-mixing bioreactors", COMMUNICATIONS BIOLOGY, vol. 4, no. 1, 1 December 2021 (2021-12-01), XP055947031, DOI: 10.1038/s42003-021-02719-5
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
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