Title:
T CELL RECEPTOR RECOGNISING KRAS MUTATION AND ENCODING SEQUENCE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/068938
Kind Code:
A1
Abstract:
Provided is a T cell receptor (TCR) capable of specifically binding to KRAS G12V mutant antigens; the mutant antigen short peptide VVGAVGVGK is capable of forming a complex with HLA A1101, and the TCR specifically binds to said complex. Also provided in the present invention are a nucleic acid molecule encoding the TCR and a vector comprising the nucleic acid molecule. Also provided are TCR-transduced cells.
Inventors:
HU JING (CN)
SUN HANLI (CN)
SUN HANLI (CN)
Application Number:
PCT/CN2020/120191
Publication Date:
April 15, 2021
Filing Date:
October 10, 2020
Export Citation:
Assignee:
XLIFESC LTD (CN)
International Classes:
C07K14/725; A61K38/17; A61P35/00; A61P37/02; C12N5/078; C12N5/10; C12N15/12; C12N15/867
Domestic Patent References:
| WO2014206304A1 | 2014-12-31 |
Foreign References:
| CN110272482A | 2019-09-24 | |||
| CN107223134A | 2017-09-29 | |||
| CN108395479A | 2018-08-14 |
Other References:
Q. J. WANG, Z. YU, K. GRIFFITH, K.-I. HANADA, N. P. RESTIFO, J. C. YANG: "Identification of T-cell Receptors Targeting KRAS-Mutated Human Tumors", CANCER IMMUNOLOGY RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 4, no. 3, 1 March 2016 (2016-03-01), US, pages 204 - 214, XP055314168, ISSN: 2326-6066, DOI: 10.1158/2326-6066.CIR-15-0188
Q. J. WANG, Z. YU, K. GRIFFITH, K.-I. HANADA, N. P. RESTIFO, J. C. YANG: "Identification of T-cell Receptors Targeting KRAS-Mutated Human Tumors", CANCER IMMUNOLOGY RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 4, no. 3, 1 March 2016 (2016-03-01), US, pages 204 - 214, XP055314168, ISSN: 2326-6066, DOI: 10.1158/2326-6066.CIR-15-0188
FANGJUN CHEN, ZOU ZHENGYUN, DU JUAN, SU SHU, SHAO JIE, MENG FANYAN, YANG JU, XU QIUPING, DING NAIQING, YANG YANG, LIU QIN, WANG QI: "Neoantigen identification strategies enable personalized immunotherapy in refractory solid tumors", THE JOURNAL OF CLINICAL INVESTIGATION, B M J GROUP, GB, vol. 129, no. 5, 1 May 2019 (2019-05-01), GB, pages 2056 - 2070, XP055717584, ISSN: 0021-9738, DOI: 10.1172/JCI99538
CHATANI PRAVEEN D., YANG JAMES C.: "Mutated RAS: Targeting the “Untargetable” with T Cells", CLINICAL CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 26, no. 3, 1 February 2020 (2020-02-01), US, pages 537 - 544, XP055799949, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-19-2138
CHIN J LAB MED, vol. 35, no. 11, November 2012 (2012-11-01)
TUMOUR BIOL, vol. 37, no. 5, May 2016 (2016-05-01), pages 6823 - 30
J CANCER, vol. 10, no. 4, 2019, pages 821 - 828
BR J CANCER, vol. 113, no. 8, 20 October 2015 (2015-10-20), pages 1206 - 1215
CHUNG ET AL., PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 12654 - 12658
KOPPE ET AL., CANCER METASTASIS REVIEWS, vol. 24, 2005, pages 539
CHAUDHARY ET AL., NATURE, vol. 339, 1989, pages 394
EPEL ET AL., CANCER IMMUNOLOGY AND IMMUNOTHERAPY, vol. 51, 2002, pages 565
GILLIES ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (PNAS, vol. 89, 1992, pages 1428
CARD ET AL., CANCER IMMUNOLOGY AND IMMUNOTHERAPY, vol. 53, 2004, pages 345
HALIN ET AL., CANCER RESEARCH, vol. 63, 2003, pages 3202
MOSQUERA ET AL., THE JOURNAL OF IMMUNOLOGY, vol. 174, 2005, pages 4381
ZHU ET AL., INTERNATIONAL JOURNAL OF CANCER, vol. 62, 1995, pages 319
LAPOTKO ET AL., CANCER LETTERS, vol. 239, 2005, pages 36
HUANG ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 128, 2006, pages 2115
PENG ET AL., GENE THERAPY, vol. 11, 2004, pages 1234
MAMOT ET AL., CANCER RESEARCH, vol. 65, 2005, pages 11631
ROBBINS ET AL., J. IMMUNOL., vol. 180, 2008, pages 6116 - 6131
ROSENBERG ET AL., NAT REV CANCER, vol. 8, no. 4, 2008, pages 299 - 308
SAMBROOKRUSSELL ET AL.: "Molecular Cloning-A Laboratory Manual", 2001, CSHL PUBLISHING COMPANY
SAMBROOKRUSSELL: "Molecular Cloning a Laboratory Manual"
See also references of EP 4043486A4
Q. J. WANG, Z. YU, K. GRIFFITH, K.-I. HANADA, N. P. RESTIFO, J. C. YANG: "Identification of T-cell Receptors Targeting KRAS-Mutated Human Tumors", CANCER IMMUNOLOGY RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 4, no. 3, 1 March 2016 (2016-03-01), US, pages 204 - 214, XP055314168, ISSN: 2326-6066, DOI: 10.1158/2326-6066.CIR-15-0188
FANGJUN CHEN, ZOU ZHENGYUN, DU JUAN, SU SHU, SHAO JIE, MENG FANYAN, YANG JU, XU QIUPING, DING NAIQING, YANG YANG, LIU QIN, WANG QI: "Neoantigen identification strategies enable personalized immunotherapy in refractory solid tumors", THE JOURNAL OF CLINICAL INVESTIGATION, B M J GROUP, GB, vol. 129, no. 5, 1 May 2019 (2019-05-01), GB, pages 2056 - 2070, XP055717584, ISSN: 0021-9738, DOI: 10.1172/JCI99538
CHATANI PRAVEEN D., YANG JAMES C.: "Mutated RAS: Targeting the “Untargetable” with T Cells", CLINICAL CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 26, no. 3, 1 February 2020 (2020-02-01), US, pages 537 - 544, XP055799949, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-19-2138
CHIN J LAB MED, vol. 35, no. 11, November 2012 (2012-11-01)
TUMOUR BIOL, vol. 37, no. 5, May 2016 (2016-05-01), pages 6823 - 30
J CANCER, vol. 10, no. 4, 2019, pages 821 - 828
BR J CANCER, vol. 113, no. 8, 20 October 2015 (2015-10-20), pages 1206 - 1215
CHUNG ET AL., PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 12654 - 12658
KOPPE ET AL., CANCER METASTASIS REVIEWS, vol. 24, 2005, pages 539
CHAUDHARY ET AL., NATURE, vol. 339, 1989, pages 394
EPEL ET AL., CANCER IMMUNOLOGY AND IMMUNOTHERAPY, vol. 51, 2002, pages 565
GILLIES ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (PNAS, vol. 89, 1992, pages 1428
CARD ET AL., CANCER IMMUNOLOGY AND IMMUNOTHERAPY, vol. 53, 2004, pages 345
HALIN ET AL., CANCER RESEARCH, vol. 63, 2003, pages 3202
MOSQUERA ET AL., THE JOURNAL OF IMMUNOLOGY, vol. 174, 2005, pages 4381
ZHU ET AL., INTERNATIONAL JOURNAL OF CANCER, vol. 62, 1995, pages 319
LAPOTKO ET AL., CANCER LETTERS, vol. 239, 2005, pages 36
HUANG ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 128, 2006, pages 2115
PENG ET AL., GENE THERAPY, vol. 11, 2004, pages 1234
MAMOT ET AL., CANCER RESEARCH, vol. 65, 2005, pages 11631
ROBBINS ET AL., J. IMMUNOL., vol. 180, 2008, pages 6116 - 6131
ROSENBERG ET AL., NAT REV CANCER, vol. 8, no. 4, 2008, pages 299 - 308
SAMBROOKRUSSELL ET AL.: "Molecular Cloning-A Laboratory Manual", 2001, CSHL PUBLISHING COMPANY
SAMBROOKRUSSELL: "Molecular Cloning a Laboratory Manual"
See also references of EP 4043486A4
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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