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Title:
TETRAHYDROPYRAN-BASED THIODISACCHARIDE MIMICS AS GALECTIN-3 INHIBITORS
Document Type and Number:
WIPO Patent Application WO/2019/241461
Kind Code:
A1
Abstract:
The present disclosure relates to compounds of Formula (1), which inhibit Gal-3, and include pharmaceutically acceptable salts, compositions comprising such compounds, and methods making and using such compounds and compositions.

Inventors:
HARTZ RICHARD A (US)
XU LI (US)
YOON DAVID S (US)
REGUEIRO-REN ALICIA (US)
JALAGAM PRASADA RAO (IN)
PANDA MANORANJAN (IN)
NAIR SATHEESH KESAVAN (IN)
Application Number:
PCT/US2019/036877
Publication Date:
December 19, 2019
Filing Date:
June 13, 2019
Export Citation:
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Assignee:
SQUIBB BRISTOL MYERS CO (US)
International Classes:
C07H17/02; A61K31/7048; A61K31/7052; A61P29/00; A61P43/00; C07H17/04
Domestic Patent References:
WO2016005311A12016-01-14
WO2010126435A12010-11-04
WO2014067986A12014-05-08
WO2005113568A12005-12-01
WO2005113569A12005-12-01
WO2014067986A12014-05-08
WO2017080971A12017-05-18
WO2016120403A12016-08-04
WO2013003383A12013-01-03
Foreign References:
US20140067986A12014-03-06
US20140099319A12014-04-10
Other References:
CELL, vol. 76, pages 597 - 598
IMMUNOLOGICAL REVIEWS, vol. 230, pages 160 - 171
CIRCULATION, vol. 110, pages 3121 - 3128
J PHARMACOL EXP THER, vol. 351, pages 336 - 343
AM J. RESPIR . CRIT. CARE MED., vol. 185, pages 537 - 546
PNAS, vol. 103, pages 5060 - 5065
AM. J. PATHOL., vol. 172, pages 288 - 298
CURR. HEART FAIL. REP., vol. 7, pages 1 - 8
"ACS Symposium Series", vol. 1115, article "Galectin-3C: Human Lectin for Treatment of Cancer", pages: 195 - 23
"Galectin Therapeutics", 7 February 2017, PRESS RELEASE
"Remington's Pharmaceutical Sciences", 1985, MACK PUBLISHING COMPANY
OHLSSON, JMAGNUSSON, G, CATRBOHYDR. RES, vol. 329, 2000, pages 49
Attorney, Agent or Firm:
SUN, Jing G. et al. (US)
Download PDF:
Claims:
CLAIMS

We claim:

1 A compound of Formula i

or a pharmaceutically acceptable salt thereof, wherein:

A is O, NH or N(COCH3);

R2 is alkyl, (C02(R5))alkyl, or (CON(R&)(R '))alkyl,

R3 is cycloalkyl, tetrahydropyranyl, or Ar1, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R3, and (R6)(R7)N;

R4 is alkyl, cycloalkyl, bicyclo[2.2. l-2]alkyl, or Ar2, and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and

CO2R5;

R5 is hydrogen or alkyl;

R6 is hydrogen, alkyl, alkyl carbonyl, or phenyl;

R7 is hydrogen or alkyl; or (R°)(R7)N taken together is azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl dioxide, and is substituted with 0-3 substituents selected from halo, alkyl, hydroxy, and alkoxy;

Ar1 is phenyl, thiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, or pyrazinyl; and

Ar2 is phenyl, biphenyl, naphthalenyl, thiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, quinolinyl, isoquinolinyl, or quinoxalinyl.

2. A compound of claim 1, wherein the compound is of Formula (la):

or a pharmaceutically acceptable salt thereof.

3. A compound of claim 1 or claim

4. A compound of claim 1 or claim 2 where R2 is alkyl.

5 A compound of claim 1 or claim 2 where R2 is (C02(R5))alkyl or

(CON(R6)(R7))alkyl .

6. A compound of claim 1 or claim 2 where R3 is Ar1 and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R5, and (R."){ R }\.

7. A compound of clai 1 or claim 2 where RJ is cycloalkyl or ietrahydropyrany! and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CQ2R5, and (R°)(R7)N.

8. A compound of claim 1 or claim 2 where R4 is Ar2 and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R5.

9. A compound of claim 1 or claim 2 where R4 is alkyl, cycloalkyl, or bicyclo[2.2 l~ 2]aikyl, and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R5.

10. A compound of claim 1 or claim 2 where Ar1 is phenyl, pyridinyl, pyridaziny!, pyrimidinyl, or pyrazinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R5, and (R6)(R')N.

11. A compound of claim 1 or claim 2 where Ar2 is phenyl, pyridinyl, pyridazinyl, pyrimidinyl, or pyrazinyl, and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO :R

12. A composition comprising a therapeutically effective amount of a compound of any one of claims 1 to 11, or a pharmaceutically acceptable salt thereof, and a

pharmaceutically acceptable carrier

13. A method for treating fibrosis of organs (including liver, kidney, lung, heart and skin), liver diseases and conditions (including acute hepatitis, chronic hepatitis, liver fibrosis, liver cirrhosis, portal hypertension, regenerative failure, non-alcoholic steatohepatitis (NASH), liver hypofunction, and hepatic blood flow disorder), cell proliferative diseases, cancers, and conditions (including solid tumor, solid tumor metastasis, vascular fibroma, myeloma, multiple myeloma, Kaposi's sarcoma, leukemia, chronic lymphocytic leukemia (CLL)) and invasive metastasis of cancer ceil), inflammatory diseases and conditions (including psoriasis, nephropathy, and pneumonia), gastrointestinal tract diseases and conditions (including irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and abnormal pancreatic secretion), renal diseases and conditions, urinary tract-associated diseases and conditions (including benign prostatic hyperplasia or symptoms associated with neuropathic bladder disease, spinal cord tumor, hernia of intervertebral disk, spinal canal stenosis, and symptoms derived from diabetes), lower urinary tract diseases and conditions (including obstruction of lower urinary tract), inflammatory diseases and conditions of lower urinary tract (including dysuria and frequent urination), pancreatic diseases and conditions, abnormal

angiogenesis-associated diseases and conditions (including arterial obstruction), scleroderma, brain-associated diseases and conditions (including cerebral infarction and cerebral hemorrhage), neuropathic pain and peripheral neuropathy, ocular diseases and conditions (including age-related macular degeneration (AMD), diabetic retinopathy, proliferative vitreoretinopathy (PVR), cicatricial pemphigoid, and glaucoma filtration surgery scarring) comprising administering a therapeutically effective amount of a compound of any one of claims 1 to 11, or a pharmaceutically acceptable salt thereof, to a patient.

14. The method of claim 13 where the disease or condition is renal fibrosis, pulmonary fibrosis, hepatic fibrosis, arterial fibrosis, or systemic sclerosis.

Description:
TETRAHYDROPYRAN-BASED THIODI S AC CH ARIDE MIMICS

AS GALECTIN-3 INHIBITORS

B ACKGROUND OF THE INVENTION

Galectin-3 (Gal-3) is a b-ga!actoside binding lectin of about 30 KDa(Cell 76: 597- 598), that is involved in the regulation of inflammatory and fibrotic processes.

(Immunological Reviews 230: 160-171). Under uncontrolled inflammation and pro- fibrotic condition, Gal-3 promotes fibroblast proliferation and transformation and mediates collagen production (Circulation 110:3121-3128).

Gal-3 is Jocalyzed in many cellular location such as cytoplasm, nucleus, and cell surface. Gal-3 is also secreted by various cell types, mainly macrophages and monocytes into the blood stream (J Pharmacol Exp Ther 351 : 336—343 ) . There are multiple lines of evidence in the literature supporting the involment of Gal-3 in the development of fibrotic process in multiple organs such as lung (Am J. Respir. Crit. Care Med. 185: 537-546), liver (PNAS 103:5060-5065) and kidney (Am. J. Pathol. 172:288-298). Gal-3 has also been identified as a biomarker for heart failure indicating that modulation of Gal-3 has potential uses in the treatment of heart failure (Curr. Heart Fail. Rep. 7: 1-8). Modulation of Gal-3 can be used in the treatment of cancer since Gal -3 is involved in cell growth and differentiation playing a critical role in angiogenic, apoptotic, and metastatic pathways (Galectin-3 C: Human Lectin for Treatment of Cancer. ACS Symposium Series, Vol.

1115. Chapter 12, 195-23). Recently, Gal-3 inhibitors have proven to have positive effects when used in combination immunotherapy (Galectin Therapeutics. Press Release, February 7, 2017).

Several publications and patent applications describe synthetic inhibitors of Gal-3 that are being explored as antifibrotic agents. Recent examples of these approach are W02005113568, W02005113569, US2014067986, WO2014067986, W02017080971, WO2016120403, US20140099319 and WO2014067986. DESCRIPTION OF THE INVENTION

The present disclosure relates to compounds of the present invention, which inhibit Gal -3, and include pharmaceutically acceptable salts, compositions comprisinj such compounds, and methods making and using such compounds and compositions.

One aspect of the invention is a compound of Formula (I):

or a pharmaceutically acceptable salt thereof, wherein:

A is O, NH or Ni COC i h):

alkyl;

R 3 is cycloalkyl, tetrahydropyranyl, or Ar 1 , and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R 5 , and (R 6 )(R 7 )N;

R 4 is alkyl, cycloalkyl, bicyclo[2.2. l-2]alkyl, or Ar 2 , and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CCkR 5 ;

R s is hydrogen or alkyl;

R 6 is hydrogen, alkyl, alkylcarbonyl, or phenyl; R 7 is hydrogen or alkyl; or (R 6 )(R / )N taken together is azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiornorpholinyl dioxide, and is substituted with 0-3 substituents selected from halo, alkyl, hydroxy, and alkoxy;

Ar 1 is phenyl, thiazolyl, pyridinyJ, pyridazinyl, pyrimidinyl, or pyrazinyl; and

Ar 2 is phenyl, biphenyl, naphthalenyl, thiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, quinolinyl, isoquinolinyl, or quinoxa!inyl.

Another aspect of the invention is a compound of Formula (la):

or a pharmaceutically acceptable salt thereof, wherein:

R 2 is alkyl, (C02.(R 5 ))alkyl, or (CON(R 6 )(R '))alkyl;

R 3 is cycloalkyl, tetrahydropyranyl, or Ar 1 , and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R 5 , and (R 6 )(R 7 )N;

R 4 is alkyl, cycloalkyl, bicyclo[2.2.1 -2]al kyl , or Ar 2 , and i s substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R 5 ; R 5 is hydrogen or alkyl;

R 6 is hydrogen, alkyl, alkyl carbonyl, or phenyl; R 7 is hydrogen or alkyl; or (R 6 )(R 7 )N taken together is azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholiny! dioxide, and is substituted with 0-3 substituents selected from halo, alkyl, hydroxy, and alkoxy;

Ar 1 is phenyl, thiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, or pyrazinyl; and

Ar 2 is phenyl, biphenyl, naphthalenyl, thiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, quinolinyi, isoquinolinyl, or quinoxalinyl.

Another aspect of the invention is a compound of Formula (I) or (la) wherein R 1

Another aspect of the invention is a compound of Formula (I) or (la) wherein R 2 is alkyl.

Another aspect of the invention is a compound of Formula (I) or (la) wherein R 2 is iCCMRAiaikvi. Another aspect of the invention is a compound of Formula (I) or (la) wherein R 2 is

(CON(R 6 )(R 7 ))alkyl

Another aspect of the invention is a compound of Formula (I) or (la) wherein R 3 is Ar 1 and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CChR’, and (R 6 )(R')N. Another aspect of the invention is a compound of Formula (I) or (la) wherein R 3 is cycloalkyl or tetrahydropyranyl and is substituted with 0-3 substituents selected from eyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CCteR 3 , and (R 6 )(R')N.

Another aspect of the invention is a compound of Formula (I) or (la) wherein R 4 is Ar 2 and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R 5 . Another aspect of the invention is a compound of Formula (I) or (la) wherein R 4 is alkyl, cycloalkyl, or bicyclo[2.2 1 ~2]alkyl, and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R 5 .

Another aspect of the invention is a compound of Formula (I) or (la) wherein Ar 1 is phenyl, pyridinyl, pyridazinyl, pyrimidinyl, or pyrazinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, CO2R 5 , and (R 6 )(R 7 )N.

Another aspect of the invention is a compound of Formula (I) or (la) wherein Ar 2 is phenyl, pyridinyl, pyridazinyl, pyrimidinyl, or pyrazinyl, and is substituted with 0-5 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, haloalkoxy, and CO2R 5 .

For a compound of Formula I, the scope of any instance of a variable substituent, including R ! , R 2 , R 3 , R 4 , R 5 , R fJ , R 7 , Ar '1 , and Ar 2 , can be used independently with the scope of any other instance of a variable substituent. As such, the invention includes combinations of the different aspects.

Unless specified otherwise, these terms have the following meanings.“Alkyl” means a straight or branched alkyl group composed of 1 to 6 carbons.“Alkenyl” means a straight or branched alkyl group composed of 2 to 6 carbons with at least one double bond. “Alkynyl” means a straight or branched alkyl group composed of 2 to 6 carbons with at least one triple bond. “Cycloalkyl” means a monocyclic ring system composed of 3 to 7 carbons. Terms with a hydrocarbon moiety (e.g. alkoxy) include straight and branched isomers for the hydrocarbon portion which are composed of 1 to 6 carbons. “Halo” includes tluoro, chloro, bromo, and iodo. “Haloaikyi” and“haloaikoxy” include all halogenated isomers from monohalo to perhalo“Aryl” means a monocyclic or bicyclic aromatic ring system having 5 to 12 carbon atoms wherein one or both of the rings are aromatic. Representative examples of aryl groups include, but are not limited to, indanyl, indenyl, naphthyl, phenyl, and tetrahydronaphthyl. “Heteroaryl” means a 5 to 7 membered monocyclic or 8 to 1 1 membered bicyclic aromatic ring system with 1-5 heteroatoms independently selected from nitrogen, oxygen, and sulfur. Where a bonding attachment location is not specified, the bonding may be attached at any appropriate location as understood by practitioners in the art. Combinations of substituents and bonding patterns are only those that result in stable compounds as understood by practitioners in the art. Parenthetic and multiparenthetic terms are intended to clarify bonding relationships to those skilled in the art. For example, a term such as ((R)alkyl) means an alkyl substituent further substituted with the substituent R.

The invention includes all pharmaceutically acceptable salt forms of the compounds. Pharmaceutically acceptable salts are those in which the counter ions do not contribute significantly to the physiological activity or toxicity of the compounds and as such function as pharmacological equivalents. These salts can be made according to common organic techniques employing commercially available reagents. Some anionic salt forms include acetate, acistrate, besylate, bromide, chloride, citrate, fumarate, glucouronate, hydrobromide, hydrochloride, hydroiodide, iodide, lactate, maleate, mesylate, nitrate, pamoate, phosphate, succinate, sulfate, tartrate, tosylate, and xinofoate. Some cationic salt forms include ammonium, aluminum, benzathine, bismuth, calcium, choline, diethy!amine, diethanolamine, lithium, magnesium, meglumine,

4-phenylcyclohexylamine, piperazine, potassium, sodium, tromethamine, and zinc. Some of the compounds of the invention exist in stereoisomeric forms. The invention includes all stereoisomeric forms of the compounds including enantiomers and diastereomers. Methods of making and separating stereoisomers are known in the art. The invention includes ail tautomeric forms of the compounds. The invention includes atropi somers and rotational isomers.

The invention is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include deuterium and tritium. Isotopes of carbon include l3 C and 14 C. Isotopically- labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopica!ly-labe!ed reagent in place of the non-labeled reagent otherwise employed. Such compounds may have a variety of potential uses, for example as standards and reagents in determining biological activity. In the case of stable isotopes, such compounds may have the potential to favorably modify biological, pharmacological, or pharmacokinetic properties.

BIOLOGICAL METHODS

ASSAY BUFFER Composition: 25 mM HEPES, 100 mM NaCl, 0.005% Tween 20, 0.05% BSA prepared in sterile water (all reagents from Sigma)

CONTROLS:

Positive Control: 100% DM SO (Imΐ) + His-tagged hGal-3 (20 pL)+ B-ASF (20m1)+ Anti- His Terbium Antibody (5m1) + Strep d2 Antibody (5m1).

Negative Control: 100% DMSO (Imΐ) + His-tagged hGal-3(20pL) +Anti His Terbium Antibody (5m1) + Strep d2 Aantibody (5m1).

PROTOCOL: The Gal-3 assays were performed in 384 white Opti plates in three replicates at room temperature with gentle shaking at 250-300 rpm. From the original stocks, 2.525X working stock concentrations of His- tagged recombinant human Gal-3 (hGal-3) and that of B-ASF w^ere prepared. From the working stock, 20 mΐ of hGal-3 (15 nM) and 20 mΐ B-ASF (15 nM) w^ere added to the plates. In Negative Control, only hGal- 3 was added. A concentration range of 5 Ox working stocks were prepared for the compounds in 100% DMSO. Aliquots of luL of the compounds were added to the wells and pre-incubated with 20 m! hGal-3 per well for 30 minutes Then 20 mί B-ASF wereadded and incubated for another 1 hour. To detect the signal, 5pL (final cone of l.OnM) terbium labelled Anti-His antibody was added and incubated for 30 min followed by adding 5pL (final cone of 20nM) Streptavidin d2 and incubation for another 1 hour . The assay signal was detected using HTRF screen protocol (Excitation wavelength = 340nm, emission wavelength = 615nm/665nm) on Envision 2104 Multilabel Reader.

Data analysed using Toolset and Curve Master. Results are reported in the experimental section (ICso in mM). PHARMACEUTICAL COMPOSTION AND METHODS OF USE

The compounds of this invention inhibit Gal-3. Accordingly, another aspect of the invention is a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

Another aspect of the invention is a method for treating a patient afflicted with a disease or condition selected from fibrosis of organs (including liver, kidney, lung, heart and skin), liver diseases and conditions (including acute hepatitis, chronic hepatitis, liver fibrosis, liver cirrhosis, portal hypertension, regenerative failure, non-alcoholic steatohepatitis (NASH), liver hypofunction, and hepatic blood flow disorder), cell proliferative diseases, cancers, and conditions (including solid tumor, solid tumor metastasis, vascular fibroma, myeloma, multiple myeloma, Kaposi's sarcoma, leukemia, chronic lymphocytic leukemia (CLL)) and invasive metastasis of cancer ceil),

inflammatory diseases and conditions (including psoriasis, nephropathy, and pneumonia), gastrointestinal tract diseases and conditions (including irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and abnormal pancreatic secretion), renal diseases and conditions, urinary tract-associated diseases and conditions (including benign prostatic hyperplasia or symptoms associated with neuropathic bladder disease, spinal cord tumor, hernia of intervertebral disk, spinal canal stenosis, and symptoms derived from diabetes), lower urinary tract diseases and conditions (including obstruction of lower urinary tract), inflammatory diseases and conditions of lower urinary tract (including dysuria and frequent urination), pancreatic diseases and conditions, abnormal

angiogenesis-associated diseases and conditions (including arterial obstruction), scleroderma, brain-associated diseases and conditions (including cerebral infarction and cerebral hemorrhage), neuropathic pain and peripheral neuropathy, ocular diseases and conditions (including age-related macular degeneration (AMD), diabetic retinopathy, proliferative vitreoretinopathy (PVR), cicatricial pemphigoid, and glaucoma filtration surgery scarring) with a compound of the present invention.

Another aspect of the invention is a method of treating renal fibrosis, pulmonary fibrosis, hepatic fibrosis, arterial fibrosis and systemic sclerosis comprising administering to a compound of the present invention to a patient. Another aspect of the invention is a method for treating fibrosis of organs

(including liver, kidney, lung, heart and skin) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating liver diseases and conditions (including acute hepatitis, chronic hepatitis, liver fibrosis, liver cirrhosis, portal hypertension, regenerative failure, non-alcoholic steatohepatitis (NASH), liver hypofunction, and hepatic blood flow disorder) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating cell proliferative diseases, cancers, and conditions (including solid tumor, solid tumor metastasis, vascular fibroma, myeloma, multiple myeloma, Kaposi's sarcoma, leukemia, chronic lymphocytic leukemia (CLL)) and invasive metastasis of cancer cell) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating inflammatory diseases and conditions (including psoriasis, nephropathy, and pneumonia) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating gastrointestinal tract diseases and conditions (including irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and abnormal pancreatic secretion) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating renal diseases and conditions comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating urinary tract-associated diseases and conditions (including benign prostatic hyperplasia or symptoms associated with neuropathic bladder disease, spinal cord tumor, hernia of intervertebral disk, spinal canal stenosis, and symptoms derived from diabetes) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating lower urinary tract diseases and conditions (including obstruction of lower urinary tract), inflammatory diseases and conditions of lower urinary' tract (including dysuria and frequent urination) compri sing administeri ng to a compound of the present invention to a patient. Another aspect of the invention is a method for treating pancreatic diseases and conditions comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating abnormal angiogenesis- associated diseases and conditions (including arterial obstruction) comprising

administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating brain-associated diseases and conditions (including cerebral infarction and cerebral hemorrhage) comprising administering to a compound of the present invention to a patient.

Another aspect of the invention is a method for treating neuropathic pain and peripheral neuropathy comprising administering to a compound of the present invention to a patient

Another aspect of the invention is a method for treating ocular diseases and conditions (including age-related macular degeneration (AMD), diabetic retinopathy, proliferative vitreoretinopathy (PVR), cicatricial pemphigoid, and glaucoma filtration surgery scarring) comprising administering to a compound of the present invention to a patient.

The compound s of the invention may be used in the treatment and/or prophylaxis of conditions in which Gal -3 plays a role.

The compounds of the present invention may be used for the manufacture of a medicament for the treatment and/or prophylaxis of a condition in which inhibition of the physiological activity of Gal-3 is useful, such as diseases in which a Gal-3 receptor participates, is involved in the etiology or pathology of the disease, or is otherwise associated with at least one symptom of the disease.

The compounds of the invention can be used alone, in combination with other compounds of the present invention, or in combination with one or more, preferably one to two other agent(s).

“Therapeutically effective” means the amount of agent required to provide a meaningful patient benefit as understood by practitioners in the field of pain.

“Patient” means a person afflicted with pain and suitable for therapy as understood by practitioners in the field.

“Treatment,”“therapy,”“regimen,” and related terms are used as understood by practitioners in the field. The compounds of this invention are generally given as pharmaceutical compositions comprised of a therapeutically effective amount of a compound of the present invention or its pharmaceutically acceptable salt and a pharmaceutically acceptable carrier and may contain conventional excipients. A therapeutically effective amount is that which is needed to provide a meaningful patient benefit. Pharmaceutically acceptable carriers are those conventionally known carriers having acceptable safety profiles. Compositions encompass all common solid and liquid forms including capsules, tablets, losenges, and powders as well as liquid suspensions, syrups, elixers, and solutions. Compositions are made using common formulation techniques, and

conventional excipients (such as binding and wetting agents) and vehicles (such as water and alcohols) are generally used for compositions. See, for example, Remington’s Pharmaceutical Sciences, 17th edition, Mack Publishing Company, Easton, PA (1985).

Solid compositions are normally formulated in dosage units and compositions providing from about 1 to 1000 mg of the active ingredient per dose are preferred. Some examples of dosages are l mg, 10 mg, 100 mg, 250 mg, 500 mg, and 1000 mg.

Generally, other antiretroviral agents will be present in a unit range similar to agents of that class used clinically. Typically, this is 0.25-1000 mg/unit.

Liquid compositions are usually in dosage unit ranges. Generally, the liquid composition will be in a unit dosage range of 1-100 mg/mL. Some examples of dosages are 1 mg/mL, 10 mg/mL, 25 mg/mL, 50 mg/mL, and 100 mg/mL.

The invention encompasses all conventional modes of administration; oral and parenteral methods are preferred. Generally, the dosing regimen will be similar to other agents used clinically. Typically, the daily dose will be 1-100 mg/kg body weight daily. Generally, more compound is required orally and less parenterally. The specific dosing regime, however, will be determined by a physician using sound medical judgement.

CHEMICAL METHODS

The compounds of this i nvention can be made by various methods known in the art including those of the following schemes and in the specific embodiments section. The structure numbering and variable numbering shown in the synthetic schemes are distinct from, and should not be confused with, the structure or variable numbering in the claims or the rest of the specification. The variables in the schemes are meant only to illustrate how to make some of the compounds of this invention. The disclosure is not limited to the foregoing illustrative examples and the examples should be considered in all respects as illustrative and not restrictive, reference being made to the claims, rather than to the foregoing examples, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced.

SECTION A

Analytical LC-MS/HPLC retention time reported for each example and intermediate uses one of the following general analytical LC-MS/HPLC condition

LC/MS Methods:

LC/MS Method 1

Start %B = 0, Final %B = 100 over 2 minute gradient; hold at 100%B for 1 min

Flow Rate ::: 0.8 mL/min

Detector Wavelength ::: 254 ntn

Solvent A = 90% water, 10% methanol, 0.1% TFA

Solvent B ::: 10% water, 90% methanol, 0.1% TFA

Column = BEH C18 2.1 X 50 mm, 1.7 itm

Oven temp = 40 °C

LC/MS Method 2

Start %B = 0, Final %B = 100 over 2 minute gradient; hold at !00%B for 1 min

Flow Rate ::: 0.8 mL/min

Detector Wavelength = : 220 nm

Solvent A = 90% water, 10% methanol, 0.1% TFA

Solvent B ::: 10% water, 90% methanol, 0.1% TFA

Column = BEH C18 2.1 X 50 mm, 1.7 itm

Oven temp = 40 °C

j LC/MS Method 3

Conditions: Start %B == : 0, Final %B = 100 over 4 minute gradient; hold at 100% B for 1 min Solvent A: 90% water, 10% methanol, 10 mM ammonium acetate

Solvent B: 10% water, 90% methanol, 10 mM ammonium acetate

Column: Phenomenex Luna CIS, 2.0 x 50 mm, 3 pm

Flow Rate: 0.8 mL/min

Detector Wavelength: 220 nm

LC/MS Method 4

Start %B = 0, Final %B = 100 over 2 minute gradient: hold at 100%B for 1 min

Flow Rate : = 0.8 mL/min

Detector Wavelength = 220 nm

Solvent A = 90% water, 10% acetonitrile, 0 05% TFA

Solvent B = 10% water, 90% acetonitrile, 0.05% TFA

Column = BEH C18 2.1 x 50 mm, 1.7 pm particles

Preparative HPLC Methods

Prep HPLC Method 1

Start %B == : 5, %B == : 50 over 15 min gradient, Final %B = 100 over 3 additional min gradient; hold at 100% B for 5 min

Flow 7 Rate ::: 40 mL/min

Detector Wavelength = 254 nm

Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B = 95% acetonitrile, 5% water 0.1% TFA

Column = Waters Sunfire 30 x 150 mm, 5 pm, C18

Prep HPLC Method 2

Start %B = 5, Final %B == : 100 over 20 min gradient, hold at 100% B for 5 min

Flow Rate = 40 mL/min

Detector Wavelength = 254 nm

Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B = 95% acetonitrile, 5% water 0.1% TFA Column = Waters Sunfire 30 x 150 mm, 5 mih, C18

Prep HPLC Method 3

Start %B ::: 5, %B ::: 60 over 15 min gradient, Final %B = 100 over 3 additional min gradient; hold at 100% B for 5 min

Flow Rate ::: 40 mL/min

Detector Wavelength = 254 nm

Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B ::: 95% acetonitrile, 5% water 0.1% TFA

Column = Waters Sunfire 30 x 150 mm, 5 pm, C18

Prep HPLC Method 4

Start %B ::: 30, Final %B ::: 100 over 15 minute gradient; hold at 100% B for 5 min Flow Rate = 40 mL/min

Detector Wavelength ::: 220 nm

Solvent A = 10% methanol, 90% water, 0.1% TFA

Solvent B = 90% methanol, 10% water 0.1% TFA

Column = Luna Axia 30 x 100 mm, 5 pm, C18 Preparative MPLC Methods

Prep MPLC Method 1

Start %B ::: 5, Final %B = 100 over 18 min gradient (14 column volumes); hold at 100% B for 4 min (3 column volumes)

Flow Rate ::: 40 mL/min

Detector Wavelength = : 254 n

Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B ::: 95% acetonitrile, 5% water 0.1% TFA

Column = 50 g Redisep Gold column, C18 Analytical HPLC Methods

Analytical HPLC Method 1

Conditions: 10% B 100% B over 15 min gradient; hold at 100% B for 10 min Solvent A: 5% acetonitrile, 95% water, 0.1% TFA

Solvent B: 95% acetonitrile, 5% water, 0.1% TFA

Column: Waters Sunfire C18, 3.0 x 150 mm, 3.5 mih

Flow Rate: 0.5 rnL / min

Detector Wavelength: 254 nm

Analytical HPLC Method 2

Conditions: 10% B -----> 100% B over 15 min gradient; hold at 100% B for 10 min Solvent A: 5% acetonitrile, 95% water, 0.1% TFA

Solvent B: 95% acetonitrile, 5%o water, 0.1% TFA

Column: Waters Xbridge phenyl, 3.0 x 150 mm, 3.5 pm

Flow Rate: 0.5 mL / min

Detector Wavelength: 254 nrn Analytical HPLC Method 3

Conditions: 10% B 100% B over 15 min gradient; hold at 100% B for 3 min Solvent A: 5% acetonitrile, 95% water, 0.1% TFA

Solvent B: 95% acetonitrile, 5% water, 0.1% TFA

Column: Xselect CSH CIS, 3.0 x 150 mm, 3.5 pm

Flow Rate: 1.0 mL / min

Detector Wavelength: 254 nm

Analytical HPLC Method 4

Conditions: 10% B -- 100% B over 15 min gradient; hold at 100% B for 3 min Solvent A: 5% acetonitrile, 95% water, 0 1 % TFA

Solvent B: 95% acetonitrile, 5% water, 0.1% TFA

Column: Z orb ax Bonus RP, 3.0 x 150 mm, 3 5 pin

Flow Rate: 1.0 mL / min

Detector Wavelength: 254 nm

Prep HPLC (LC/MS detecti on) Method 5

Start %B = 10, Final %B = 50 over 20 minute gradient; hold at 100% B for 4 min Flow Rate = 20 mL/min

Detector Wavelength = : 220 n (Fraction collection triggered by LCMS signals) Solvent A = 5% acetonitrile, 95% water, 0.1% ammonium acetate

Solvent B ::: 95% acetonitrile, 5% water 0.1% ammonium acetate

Column = Abridge CIS, 200 mm x 19 mm, 5 pm particles

Prep HPLC (LC/MS detection) Method 6

Start %B = 5, Final %B = 45 over 20 minute gradient; hold at 100% B for 4 min Flow Rate ::: 20 mL/min

Detector Wavelength = : 220 ntn (Fraction collection triggered by LCMS signals) Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B ::: 95% acetonitrile, 5% water 0.1 % TFA

Column = Abridge CIS, 200 mm x 19 mm, 5 pm particles

Prep HPLC Method 7

Start %B = 0, Final %B = 100 over 10 minute gradient; hold at 100% B for 2 min

Flow Rate ::: 40 mL/min

Detector Wavelength = 220 nm

Solvent A = 10% acetonitrile, 90% water, 0.1% TFA

Solvent B ::: 90% acetonitrile, 10% water 0.1 % TFA

Column = Phenomonex Luna AAIA CIS, 30 mm x 100 mm, 5 pm particles

Analytical LC/MS Methods

Analytical LC/MS Method 1

Start. %B = 0, Final %B = 100 over 3 minute gradient; hold at 100% B for 0.75 min Flow Rate = 1 mL/min

Detector Wavelength = 220 nm

Solvent A = 5% acetonitrile, 95% water, 0.1% ammonium acetate

Solvent B = 95% acetonitrile, 5% water 0.1% ammonium acetate

Column = Abridge Cl 8, 2.1 mm x 50 mm, 1.7 pm particles

Temperature ::: 50 °C Analytical LC/MS Method 2

Start. %B = 0, Final %B = 100 over 3 minute gradient; hold at 100% B for 0.75 min

Flow Rate = 1 mL/min

Detector Wavelength = 220 nm

Solvent A = 5% acetonitrile, 95% water, 0.1% TFA

Solvent B = 95% acetonitrile, 5% water 0.1% TFA

Column = Abridge Cl 8, 2.1 mm x 50 mm, 1.7 pm particles

Temperature = 50 °C

Preparation of Intermediates (1)

Preparati on of 1 -(difluoromethyl)-3 -ethynylbenzene

To a stirred solution of 3-ethynylbenzaidehyde (200 mg, 1.537 mmol) in CH2G2 (4 mL) under N2 was added DAST (0.305 mL, 2 305 mmol). The reaction mixture was stirred at rt for 24 h. The mixture was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (10 mL). The aqueous layer was extracted with dichloromethane (3 x 15 mL). The combined organic layers were washed with brine (10 mL), dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (0% 20% ethyl acetate in hexanes; 40 g column) to afford l-(difluoromethyl)-3 -ethynylbenzene (104 mg, 0.684 mmol, 45% yield) as a colorless oil. l H NMR (400 MHz, CHLOROFORM-d) d 7 66 (s, 1H), 7 62 (dd, ,/ 7.7, 1.1 Hz, 1H), 7.56 - 7.50 (m, 1H), 7.48 - 7.42 (m, 1H), 6.65 (t, J=56.2 Hz, 1H), 3.15 (s, 1H); 19 F NMR (376 MHz, CHLOROFORM-d) d -1 11.50 (s, IF); 13 C NMR (101 MHz, CHLOROFORM-d) d 134.3 (t, .7=22.7 Hz, 1C), 133.9 (t, ,7=1.9 Hz, 1 C), 128.9 (t, .7=6.2 Hz, 1C), 128.4, 125.4 (t, 7=6.2 Hz, 1C), 122.5, 113.7 (t, .7=239.3 Hz, 1C), 82.2, 77.9 Preparation of l-(difluoromethoxy)-3-ethynylbenzene

To a solution of 3-ethynylphenol (0.264 g, 2.235 mmol) in CH2CI2 (9 mL) was added aqueous potassium hydroxide (20 wt%) (3.13 mL, 13.41 mmol).

(Bromodifluoromethyl)trimethylsilane (0.556 mL, 3 58 mmol) was then added via syringe and the reaction mixture was stirred at rt for 2 h. The mixture was transferred to a separatory funnel containing water (10 rnL). The aqueous layer was extracted with ethyl acetate (3 x 15 mL). The combined organic layers were washed with brine (10 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% 20% ethyl acetate in hexanes; 40 g column) to afford l-(difluoromethoxy)-3-ethynylbenzene (195 mg, 1.160 mmol, 52% yield) as a colorless oil. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.42 - 7.32 (m, 2H), 7 28 (br s, 1H), 7.15 (dt, .7=7 1, 2.1 Hz, 1H), 6.53 (t, J=73.5 Hz, 1H), 3.14 (s, 1H); 19 F NMR (376 MHz, CHLOROFORM-d) d -80.98 (s, I F); 13 C NMR (101 MHz, CHLOROFORM-d) d 150.5, 129.4, 128.8, 123.5, 122.7, 120.0, 115.3 (t, .7=260.5 Hz, 1C), 82.0, 77.9.

Preparation of 2-ethynyl-5-fluoropyrimidine

Step 1. Preparation of 5-iluoro-2-(( trimethyl silyl)ethynyl)pyrimidine

A solution of 2-chloro-5-fluoropyrimidine (0.8 g, 6.04 mmol) and

ethynyltrimethyl silane (1.779 g, 18.1 1 mmol) in toluene (30 rnL) in a pressure vessel was degassed with Nr. Triethylamine (3.37 mL, 24.15 mmol), copper(I) iodide (0.115 g,

0.604 mmol), and bis(triphenylphosphine)palladium(II) dichloride (0.424 g, 0.604 mmol) were then added. The vessel was sealed and the reaction mixture was heated at 60 °C for

16 h. The mixture was cooled to it and was filtered through a pad of Celite. The filtrate was transferred to a separatory funnel containing saturated aqueous NaHCC solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed with brine (25 mL), dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (5% ----> 15% ethyl acetate in hexanes; 120 g column) to afford 5-fluoro-2-

((trimethylsilyi)ethynyl)pyrimidine (779 mg, 4.01 mmol, 66% yield) as a yellow' solid:

¾ NMR (400 MHz, CHLOROFORM-d) d 8.60 (s, 2H), 0.32 (s, 9H); LC/MS (ESI) m/e 195.0 [(M+H) + , calcd for C 9 H l2 FN2Si 195.1], /R = 1.81 min (Method 2). Step 2

To a mixture of 5-fluoro-2-((trimethylsilyl)ethynyl)pyrimidine (760 nig, 3.91 mmol) in MeOH (20 mL) was added anhydrous potassium carbonate (81 mg, 0.587 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was treated with saturated aqueous NH 4 Cl solution (3 mL) and 1 N HC1 (0.4 mL). The mixture was then concentrated to remove most of the methanol. The remaining liquid was transferred to a separatory funnel containing water (20 mL) and saturated aqueous NaHCCh solution (20 mL). The aqueous layer was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (10 mL), dried over MgS0 4, filtered, and concentrated. The product was purified by column chromatography on silica gel (30% 60% ethyl acetate in hexanes; 80 g column) to afford 2-ethynyl-5-fluoropyrimidine

(349 mg, 2.86 mmol, 73% yield) as a tan solid. ! H NMR (400 MHz, CHLOROFORM- d) 6 8 62 (s, 21 1). 3 15 (s, i l l): 19 F NMR (376 MHz, CHLOROFORM-d) 6 -134.28 (s, IF); LC/MS (ESI) m/e 123.0 [(M+H) + , calcd for C0H4FN2 123.0], t& = 0.71 min (Method 2).

Preparation of 2-ethynyl-5-methoxypyrimidine

To a mixture of 5-fluoro-2-((trimethylsilyl)ethynyl)pyrimidine (310 mg, 1.596 mmol) in MeOH (8 rnL) was added anhydrous potassium carbonate (33.1 mg, 0.239 mmol). The reaction mixture was stirred at rt for 14 h. LC/MS showed the formation of 2-ethynyl-5-fluoropyrimidine along with 2-ethynyl-5-methoxypyrimidine. The mixture was concentrated and was transferred to a separator}- funnel containing water (10 mL). The aqueous layer was extracted with ethyl acetate (3 x 10 rnL) The combined organic layers were w-ashed with brine (10 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (30% - 60% ethyl acetate in hexanes; 40 g column) to afford 2-ethynyl-5-fluoropyrimidine (67 mg, 0.549 mmol, 34% yield) as a tan solid and 2-ethynyl-5-methoxypyrimidine (20 mg, 0.149 mmol, 9% yield) as a white foam.

Data for 2-ethynyl-5-fluoropyrimidine:

‘H NMR (400 MHz, CHLOROFORM-d) d 8.62 (s, 2H), 3.15 (s, 1H); LC/MS (ESI) mie 123.0 [(M+H) + , calcd for CeTLFNi 123.0], t& = 0.50 min (Method 1).

Data for 2-ethynyl-5-methoxypyrimidine:

¾ NMR (400 MHz, CHLOROFORM-d) d 8.40 (s, 2H), 3.97 (s, 3H), 3.07 (s, 1 H); LC/MS (ESI) m!e 135.1 [(M+H) + , calcd for C7H7N2O 135.1], fe = 0.72 min (Method 1).

Preparation of 5-ethynyl-2-methoxypyrimidine

= TMS

Step 1 Step 2

Step 1. Preparation of 2-fluoro-5-((trimethylsilyl)ethynyl)pyrimidine

In a sealed tube, 5-bromo-2-fluoropyrimidine (175 mg, 0.989 mmol), bis(tripheny!phosphine)pailadiura(H) chloride (17.35 mg, 0.025 mmol), copper(I) iodide (4.71 mg, 0.025 mmol) and diisopropylamine (1691 mΐ, 11.87 mmol) were combined under nitrogen. Ethynyltrimethyl silane (151 mΐ, 1.088 mmol) was then added and the reaction mixture was stirred at 40 °C for 16 h. The reaction mixture was quenched with water and extracted with CH2CI2 (3 x 20 rnL). The combined organic layers were dried

9 1 over magnesium sulfate and concentrated under vacuum. The crude product was purified by column chromatography on silica gel (0% 30% ethyl acetate in hexanes; 40 g column) to afford 2-fluoro-5-((trimethylsilyl)ethynyl)pyrimidine (128 mg, 0.659 mmol, 67% yield). ¾ NMR (400 MHz, CHLOROFORM-d) 6 8.70 (d, .>=1 5 Hz, 21 1), 0.29 (s, 9H); LC/MS (ESI) m/e 195.0 [(M+H) + , calcd for C9H12FN2S1 195.1], fe = 1.89 min

(Method 2).

Step 2

In a round bottom flask, potassium hydroxide (72.2 mg, 1.287 mmol) was added to a solution of 2-fluoro-5-((trimethylsilyl)ethynyl)pyrimidine (125 mg, 0.643 mmol) in

MeOH (2 mL) and CH2CI2 (1.000 mL). The reaction was stirred at rt for 3 h. The reaction was quenched with water (10 mL) and extracted with DCM (3x 10 mL). The combined organic layers were dried over magnesium sutlate, filtered, and concentrated under vacuum. The crude product was purified by column chromatography on silica gel (0% -----> 30% ethyl acetate in hexanes; 40 g column) to afford 5-ethynyl~2- methoxypyrimidine (70 mg, 0.522 mmol, 81% yield) ¾ NMR (400 MHz,

CHLOROFORMS) d 8.63 (s, 2H), 4.05 (s, 3H), 3.29 (s, 1H); i3 C NMR (101 MHz, CHLOROFORM-d) d 163.9, 161 6, 111.4, 81.9, 54.9; LC/MS (ESI) m!e 135.0 [(M+H) + , calcd for C7H7N2O 135.0], & = 1.39 min (Method 2).

Preparation of iV-(2-ethynylpyrimidin-4-yl)acetamide

o

GMΪ

aceiyl chloride Pd(PPti3)2CI 2 , Cut TBAF

pyridine; E1 3 N ; toluene THF

CH 2 CI 2 Step 2 Step 3

Step 1

Step 1. Preparation of /V-(2-chloropyiimidin-4-yl)acetamide

In a round bottom flask, acetyl chloride (0.823 mL, 11.58 mmol) was added to a solution of 2-chloropyrimidin-4-amine (750 mg, 5.79 mmol) in pyridine (1.873 mL,

23.16 mmol) and CH2CL2 (30 mL). The reaction mixture was stirred at rt for 16 h. The reaction mixture was washed with cold 1 N HC1 (2 x 40 L), saturated aqueous sodium bicarbonate (50 mL), and brine (50 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ----> 65% ethyl acetate in hexanes; 120 g column) to afford iV-(2-chloropyrimidin-4-yl)acetamide (610 mg, 3 56 mmol, 61% yield) as a pale yellow solid l H NMR (400 MHz,

CHLOROFORM-d) 6 8.53 (d, >5.8 Hz, Hi), 8.13 (d, >5.8 Hz, 1H), 2 27 (s, 31 1 );

LC/MS (ESI) m/e 171.9 [(M+Naf, calcd for 0,1 LCTvONa 172.1], fe = 0.72 min

(Method 2).

Step 2. Preparation of JV-(2-((trimethylsilyl)ethynyl)pyrimidin-4-yl)acetamide

A solution of A/-(2-chloropyrimidin-4-yl)acetamide (240 mg, 1 .399 mmol) and ethynyltrimethylsilane (0.233 mL, 1.678 mmol) in toluene (6 mL) was degassed with N2. Triethylamine (0.780 mL, 5.59 mmol), copper(I) iodide (26.6 rng, 0. 140 mmol), and bis(triphenylphosphine)palladium(n) dichloride (98 mg, 0.140 mmol) were then added and the reaction mixture was heated at 60 °C for 16 h. The mixture was cooled to rt and was transferred to a separatory funnel containing saturated aqueous NaHCC solution (25 mL) The aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed with water (25 mL), brine (25 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (5% 50% ethyl acetate in hexanes; 40 g column) to afford N-{2- ((trimethylsilyl)ethynyl)pyrimidin-4-yl)acetamide (120 mg, 0.514 mmol, 37% yield). ¾ NMR (400 MHz, CHLOROFORM-d) 6 8.62 (d, .>5 8 Hz, H I ). 8.28 (hr s, 1 H), 8.12 (d, >5.8 Hz, IB), 2.25 (s, 3H), 0.30 (s, 91 1 ); LC/MS (ESI) mie 234.0 [(M+H) + , calcd for CiiHisNiOSi 234 1], fe = 1.77 min (Method 2)

In a sealed vial, TBAF (1.0 M in THF) (0 321 mL, 0.321 mmol) was added to a solution ofiV-(2-((trimethylsilyi)ethynyl)pyrimidin-4-yl)acetamide (50 mg, 0.214 mmol) in THF (1 .5 mL) at 0 °C. The reaction mixture was stirred at 0 °C for 1 h. The crude product was concentrated under vacuum and was purified by column chromatography on silica gel (20% -----> 80% ethyl acetate in hexanes; 24 g column) to afford N-(2- ethynylpyrimidin-4-yl)acetamide (22 mg, 0.137 mmol, 64% yield). l H NMR (400 MHz, CHLOROFORM-d) 6 8.63 (d, >5.8 Hz, i l l ), 8.16 (d, >5.8 Hz, 1H), 8 03 (hr s, 1 1 1 ), 3.12 (s, 1H), 2.26 (s, 31 1): LC/MS (ESI) m!e 162.0 [(M+Na) + , calcd for (id L.\ ;()Na 162.1], in = 0.98 min (Method 2).

Preparation of -(2-ethynyl-5-methoxypyrimidin-4-yl)acetamide

Step 1 MS

Step 1. Preparation of iV-(2-chloro-5-methoxypyrimidin-4-yl)acetamide

In a round bottom flask, acetyl chloride (0.891 mL, 12 53 mmol) was added to a solution of 2-chloro-5-methoxypyrimidin-4-amine (1 g, 6.27 mmol) and pyridine (2.027 mL, 25.07 mmol) in CH2CI2 (30 mL). The reaction mixture was stirred at rt for 16 h. LC/MS showed the formation of the mono and diacetyl ated products. The reaction mixture was diluted with CH2CI2 (20 mL) and the organic layer was washed with IN HC1 (50 mL), sat. sodium bicarbonate (50 mL) and brine (50 mL). The organic layer was dried over magnesium sulfate and concentrated under vacuum. The crude product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% MeOH/80% hexanes > 70% ethyl acetate containing 5% MeOH/20% hexanes: 120 g column) to afford -(2-chloro-5-methoxypyrimidin-4-yl)acetamide (430 mg, 2.133 mmol, 34% yield) as a white solid. ! H NMR (400 MHz, CHLOROFORM-d) d 7.98 (s, 1H), 3.99 (s, 3H), 2.66 (s, 3H), LC/MS (ESI) mie 201.9 [(M+H) + , calcd for C7H9CIN3O2 0.99 min (Method 2).

Step 2 Preparation of iV-(5-methoxy-2-((trimethylsilyl)ethynyl)pyrimidin-4-yl)acet amide A solution ofA r -(2-chloro-5-methoxypyrimidin-4-yl)acetamide (430 mg, 2.133 mmol) and ethynyltrimethylsilane (0.355 ntL, 2 56 mmol) in toluene (15 mL) was degassed with N2. Triethylamine (1.189 mL, 8.53 mmol), copper(I) iodide (40.6 mg, 0.213 mmol), and bis(triphenylphosphine)palladium(II) dichloride (150 mg, 0.213 mmol) were then added and the reaction mixture was heated at 60 °C for 16 h. The mixture was cooled to rt and was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with water (25 mL), brine (25 mL), dried over MgSC , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% -----> 80% ethyl acetate in hexanes, 80 g column) to afford A-(5-methoxy-2-((trimethylsilyl)ethynyl)pyrimidin-4-yl)aceta mide (46 mg, 0. 175 mmol, 8% yield). 4 1 NMR (400 MHz, CHLOROFORM-d) 6 8.08 (s, i l l), 4.00 (s, 3H), 2.68 (s, 3H), 0.37 - 0.25 (m, 9H); LC/MS (ESI) mie 264.0 | ( M 1 1 ) , calcd for

Ci. ! ! ;:··; \40’8i 264.1], /R = 1.71 min (Method 2). Step 3

In a round bottom flask, TBAF (1.0 M in THF) (0 262 mL, 0.262 mmol) was added to a solution of A r -(5-methoxy-2 ((trimethylsilyl)ethynyl)pyrimidin-4-yl)aeetamide (46 mg, 0.175 mmol) at 0 °C. The reaction mixture was warmed to rt and stirred for 1 h. The crude product was concentrated under vacuum and was purified by column chromatography on silica gel (20% -- 100% ethyl acetate in hexanes; 24 g column) to afford 7V-(2-ethynyl-5-methoxypyrimidin-4-yl)acetamide (21 mg, 0.110 mmol, 63% yield). “H NMR (400 MHz, CHLOROFORM-d) d 8.10 (s, 1H), 8.03 (br s, 1H), 4.01 (s, 31 !), 3.00 (s, 1 1 1), 2,68 (s, 31 !). LC/MS (ESI) m!e 192 0 [ ( M ! !) , calcd for C9H 10N3O2 192.1], /R = 0.87 min (Method 2).

Preparation of 2-ethynylthiazole

T S

Step 1 Step 2

Step 1. Preparation of 2-((trimethylsilyl)ethynyl)thiazole

A solution of 2-bromothiazole (500 mg, 3.05 mmol) and ethynyltrimethylsilane

(0.507 mL, 3.66 mmol) in toluene (15 mL) was degassed with N?.. Triethylamine (1.700 mL, 12.19 mmol), copper(I) iodide (58.1 mg, 0.305 mmol), and

bis(tripheny!phosphine)palladium(II) dichloride (214 mg, 0.305 mmol) were then added and the reaction mixture was heated at 60 °C for 16 h. The mixture was cooled to rt and was transferred to a separatory funnel containing saturated aqueous NaHCOs solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with water (25 mL), brine (25 mL), dried over MgS() 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% -- 40% ethyl acetate in hexanes; 80 g column) to afford 2- ((trimethylsilyl)ethynyl)thiazole (330 mg, 1.820 mmol, 60% yield). Ή NMR (400 MHz, CHLOROFORM-d) 6 7 82 (d, >=3.3 Hz, H i ), 7.35 (d, >=3 3 Hz, 1 1 1), 0.30 (s, 91 1).

LC/MS (ESI) mie 181.9 [(M+H) + , calcd for CsHnNSSi 182.0], in = 1.91 min (Method 2). Step 2

To a mixture of 2-((trimethylsilyl)ethynyl)thiazole (330 mg, 1 820 mmol) in MeOH (20 mL) was added anhydrous potassium carbonate (37 7 mg, 0.273 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was treated with saturated aqueous NH 4 C1 solution (5 mL) and 1 N HC1 (3 mL). The mixture was then concentrated to remove most of the methanol. The remaining liquid was transferred to a separatory funnel containing water (20 mL) and saturated aqueous NaHCCb solution (20 mL). The aqueous layer was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (10 ml.), dried over MgS() 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% -----> 50% ethyl acetate in hexanes; 40 g column) to afford 2-ethynylthiazole (82 mg, 0.751 mmol, 41% yield) as a tan solid. Ή NMR (400 MHz, CHLOROFORM-d) d 7.85 (d, .7=3.3 Hz, 1H), 7.39 id, .>=3 5 Hz, 1 1 1), 3.50 (s, 1 1 1). LC/MS (ESI) mie 109 9 [(M I I ) , ca!cd for

C5H4NS 110.0], /s = 1.08 min (Method 2).

Preparation of 5-ethynyl -2-(trifiuoromethyl)pyrimidine

Step 1 Step 2

Step 1. Preparation of 2-(trifluoromethyl)-5-((trimethylsilyl)ethynyl)pyrimidine

A solution of 5-bromo-2-(trifluoromethyl)pyrimidine (230 mg, 1.013 mmol) and ethynyltrimethylsilane (0.168 mL, 1.216 mmol) in toluene (8 mL) was degassed with N2. Triethylamine (0.565 mL, 4.05 mmol), copper(I) iodide (19.30 mg, 0.101 mmol), and bis(triphenylphosphme)palladium(II) dichloride (71 1 mg, 0.101 mmol) were then added and the reaction mixture was heated at 60 °C for 16 h. The mixture was cooled to rt and was transferred to a separatory funnel containing saturated aqueous NaHCC solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic layers wore washed wit water (25 mL), brine (25 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% -- 75% ethyl acetate in hexanes: 40 g column) to afford 2- (trifluoromeihyi)-5-((trimethylsilyl)ethynyi)pyrimidine (230 mg, 0.941 mmol, 93% yield). ¾ NMR (400 MHz, CHLOROFORM-d) d 8.91 (s, 2H), 0.30 (s, 9H); LC./MS (ESI) mie 245.0 | ( M 1 1 ) , calcd for C10H12F3N2S1 245. 1 ], hx = 2 13 min (Method 2)

Step 2

To a mixture of 2-(trifluoromethyi)-5-((trimethyIsiiyl)ethynyi)pyrimidine (130 mg, 0.532 mmol) in MeOH (5 mL) was added anhydrous potassium carbonate (11.03 mg, 0.080 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was treated with saturated aqueous NEUC! solution (10 mL) and 1 N HC1 (1 mL). The mixture was then concentrated to remove most of the methanol. The remaining liquid was transferred to a separatory' funnel containing water (20 mL) and saturated aqueous NaHCCb solution (20 mL). The aqueous layer was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (20 mL), dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (0% 40% ethyl acetate in hexanes; 24 g column) to afford 5-ethynyl-2- (trifluoromethyl)pyrimidine (25 mg, 0.145 mmol, 27% yield). ¾ NMR (400 MHz, CHLOROFORM-d) d 8 98 (s, 2H), 3 57 (s, 1H); LC/MS (ESI) mie 173.0 j ( M I S ) calcd for C7H4F3N2 173.0], h< = 1.51 min (Method 2).

Preparation of ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-5-methoxy-4-(4-(3,4,5- trifluorophenyi)- \H~ 1 ,2,3-triazol- 1 ~yl)tetrahydro-2ii-pyran-2~yl)methyi acetate

sodium ethoxide HOAc (80% , aq)

THF/MeOH

Step 8

Step 7

Step 6

Br,

CH 2 CI 2

Step 11

Step 1. Preparation of (2S,4aR,6S,7R,8R,8aR)-2-(4-methoxyphenyl)-6- (phenylihio)hexahydropyrano[3,2~d][l,3]dioxine~7,8-diol

To a solution of (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(phenylthio)tetrahydro-

2i7-pyran~3,4, 5-trio! (Ohlsson, J., Magnusson, G Carhohydr. Res., 2000, 329, 49) (29 5 g, 102 mmol) in MeCN (204 ml) (degassed with N?. (2 x)) was added 1- (dimethoxymethyl)-4-methoxybenzene (22.54 ml, 132 mmol). ? -Toluenesulfonic acid monohydrate (1 937 g, 10.18 mmol) was added and the reaction mixture was stirred under N?. for 18 h. The initially homogenous solution became a heterogeneous mixture. The mixture was concentrated to about 1/3 volume, then diluted with 1 N K2HPQ4 (aq) (200 mL). The resultant precipitate was collected by vacuum filtration, washed with water, and the product was further purified by trituration with ether. The solid was collected by vacuum filtration, and the product was dried in vacuo to afford

(4aR,6S,7R,8R,8aR)-2-(4-methoxyphenyl)-6-(phenylthio)hexa hydropyrano[3,2- £/][l,3]dioxine-7,8-diol (28.0 g, 71.7 mmol, 70% yield) as a white solid. 1H NMR (500 MHz, ME THAN GL-d 4 ) d 7.70 - 7.63 (m, 2H), 7.41 (d, J=8.8 Hz, 2H), 7.33 - 7.26 (m, 31 1), 6.92 (d, ,/ 8.5 Hz, 2! i), 5 55 (s, i l l), 4 61 (d, ./ 9. 1 Hz, 1 1 1 ), 4.25 - 4 18 (m, 2H), 4.10 (dd, .7=12.4, 1.4 Hz, 1H), 3.83 (s, 3H), 3.73 - 3.58 (m, 3H); LC/MS (ESI) mie 391.1 | (\! · 1 1) . calcd for (Nl iwO.S 391.1], h >, = 3 29 min (Method 3) Step 2. Preparation of (4aR,6S,7R,8S,8aS)-2-(4-methoxyphenyl)-6-(phenylthio)-8- (((trifluoromethyl)sulfonyl)oxy)hexahydropyrano[3,2-£f][l,3 ]dioxin-7-yl acetate

To a solution of (4aR,6S,7R,8R,8aR)-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-if][l ,3]dioxine-7,8-diol (40.0 g, 102 mmol) in CH2CI2 (427 ml) was added pyridine (33.1 ml, 410 mmol). The reaction mixture was cooled to - 10 °C. TfzO (24.23 ml, 143 mmol) was then added dropwise. The reaction mixture was held at -10 °C for 1 h, and was then allowed to slowly warm to rt. After 2 h, LCMS shows ca. 75% conversion. The reaction mixture was cooled to 0 °C, and acetyl chloride (8 74 ml, 123 mmol) was then added dropwise and the reaction mixture was allowed to reach rt overnight. The mixture was transferred to a separatory funnel and was washed with 1 N HCf (3 x 150 mL), 1 M K2HPO4 (150 mL), brine, dried over NaiSOr, filtered, and concentrated. The resultant semisolid was purified by trituration using a minimal amount of CH2CI2 to suspend the solid. Ether w'as then added and the mixture was stirred until the solid chunks were broken apart. The product was isolated by vacuum filtration and dried in vacuo to furnish (4aR,6S,7R,8S,8aS)-2-(4-methoxyphenyl)-6- (phenylthio)-8-(((trifluoromethyl)suJfonyl)oxy)hexahydropyra no[3,2-i7][l,3]dioxin-7-yl acetate (33 04 g, 58.5 mmol, 57% yield) as a white solid. The filtrate was concentrated and the residue was purified by silica gel chromatography (120 g cartridge; A = hexanes, B = ethyl acetate; 60 min grad.; 0% B to 100% B flow rate = 120 mL/min). The pure fractions were combined, concentrated and dried in vacuo to afford additional

(4aR,6S,7R,8S,8aS)-2-(4-methoxyphenyl)-6-(phenylthio)-8-

(((trifluoromethyl)sulfonyl)oxy)hexahydropyrano[3,2~ < i][l,3]dioxin-7-yl acetate (6 2 g, 10.98 mmol, 11% yield) as a pale yellow solid. The total yield was 39.24 g (68% yield).

1 H NMR (400 MHz, DMSO-de) 6 7.58 - 7 50 (m, 2H), 7.41 - 7.32 (m, 3H), 7.31 - 7 25 (m, 2H), 7.01 - 6.93 (m, 2H), 5.72 - 5.66 (m, 2H), 5.22 - 5.17 (m, 2H), 4.66 (d, 7=3.3 Hz, 1H), 4.16 (dd, .7=3.3, 1.3 Hz, 2H), 3.96 (s, 1H), 3.79 (s, 3H), 2.09 (s, 3H); LC./MS (ESI) m!e 565.0 [(M+H) + , calcd for C23H24 F3O9S2 565.1], fe = 4. 10 min (Method 3).

Step 3 Preparation of (4aR,6S,7R,8R,8aR)-8-hydroxy-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-yl acetate

To a 1 L round bottomed flask was added (4aR,6S,7R,8S,8aS)-2-(4- methoxyphenyl)-6-(phenylthio)-8-(((trifluoromethyl)sulfonyl) oxy)hexahydropyrano[3,2- ][l ,3]dioxin-7-yl acetate (15.8 g, 28.0 mmol), potassium nitrite (11.82 g, 139 mmol), and DMF (347 ml). The reaction mixture was heated to 50 °C for 18 h. The mixture was cooled and was diluted with water (1 L) and extracted with EtOAc (2 x 500 mL). The combined organic layers were washed with brine, dried over NaiSOr, fdtered, and concentrated. The residue was purified by silica gel chromatography (330 g cartridge; A = hexanes, B = ethyl acetate; 45 min grad.; 0% B to 100% B flow rate = 120 mL/min).

The pure fractions were combined, concentrated and dried in vacuo to furnish

(4aR,6S,7R,8R,8aR)-8-hydroxy-2~(4-methoxyphenyi)-6-

(phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-yl acetate (24.0 g, 42.2 mmol, 61% yield) as a pale yellow solid. ¾ NMR (400 MHz, DMSO-de) d 7.56 - 7.49 (m, 2H), 7 37 - 7 28 (m, 51 1), 7.01 - 6.90 (m, 21 1), 5 73 (d, .7=4.2 Hz, H i ), 5.55 (s, H I ), 5.20 (d, .7=10 3

Hz, 1 1 1 ). 4.77 (dd, 7=10.3, 3.1 Hz, 1 1 1 ). 4.14 - 4.01 (m, 3H), 3.98 - 3.93 (m, 1 1 1 ). 3.86 (s, 1H), 3.78 (s, 3H), 2.03 (s, 3H), LC/MS (ESI) m/e 433 1 [(M i l f , calcd for C22H25 0?S 433.1]; (R = 3.68 min (Method 3). Step 4 Preparation of (4aR,6S,7R,8R,8aS)-2-(4-methoxyphenyl)-6-(phenylthio)-8- (((tiiiluoromethyi)sulfonyl)oxy)hexahydropyrano[3,2~7][i,3]d ioxin-7-yl acetate To a solution of (4aR,6S,7R,8R,8aR)-8-hydroxy-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-t/][l,3]dioxin-7-yl acetate (3.00 g, 6.94 mmol) in CH2CI2 (50 niL) was added pyridine (2.81 mL, 34.7 mmol). The reaction mixture was cooled to -10 °C and triflic anhydride (2.344 mL, 13.87 mmol) was added slowly via syringe. The reaction mixture was stirred for 3 h while allowing it to warm up to 0 °C. The mixture was transferred to a separatory funnel containing cold 1 N HC1 (50 mL)

The aqueous layer was extracted with dichloromethane (3 x 50 mL). The combined organic layers were washed with saturated aqueous NaHCCh solution (25 mL), brine (25 mL), dried over MgSCri, filtered and concentrated to afford (4aR,6S,7R,8R,8aS)-2-(4- methoxyphenyl)-6-(phenylthio)-8-(((trifluoromethyl)sulfonyl) oxy)hexahydropyrano[3,2- d [l ,3jjdioxin-7-yl acetate (3.93 g, 6 96 mmol, 100% yield) as an amber foam. The product was used directly in the next step without further purification. ¾ NMR (400 MHz, CHLOROFORM-d) 6 7.66 - 7.61 (m, 21 1 ). 7 39 - 7.29 (m, 51 1 ), 6.93 - 6.89 (m, 2H), 5.52 (s, 1H), 5.22 - 5.18 (m, 1H), 5.15 (dd, .7=10.3, 3.0 Hz, 1H), 5.06 (d, J=10.0 Hz, 1 1 1 ).. 4.42 (dd, .7=12.5, 1.5 Hz, 1 1 1 ).. 4.24 (dd, .7=3.6, 0.9 Hz, i l l ). 4 09 (dd, ./ 1 2.7, 1.6

Hz, I I I ). 3.86 (s, 3H), 3.85 (d, ./ 1 .3 Hz, 1 H), 2.17 (s, 3H); 1.C7MS (ESI) mie 587. 1 [(M+Na) , caicd for C23H23F309S?.Na 587.1], /R = 2.21 min (Method 1).

Step 5. Preparation of (4aR,6S,7R,8S,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-i7][l,3]dioxin-7-yl acetate

A mixture of (4aR,6S,7R,8R,8aS)-2-(4-methoxyphenyi)-6-(phenylthio)-8- (((triiluoromethyi)sulfonyl)oxy)hexahydropyrano[3,2~ < i][i,3]dioxin-7-yi acetate (3 93 g, 6.96 mmol) and tetrabutylammonium azide (5.94 g, 20.88 mmol) in DMF (35 mL) was heated at 50 °C for 3 h. The mixture was transferred to a separatory funnel containing water (25 mL) and saturated aqueous NaHCCti solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 100 mL). The combined organic layers were washed with brine (50 mL), dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (30% > 50% ethyl acetate in hexanes; 220 g column) to afford (4aR,6S,7R,8S,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-i7][l,3]dioxin-7-yl acetate (1.35 g, 2.95 mmol, 42% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.65 - 7.58 (m, 2H), 7.41 - 7.29 (m, 51 1). 6.93 - 6.87 (m, 2H), 5.55 (s, 1H), 5.36 (t, .7=9.9 Hz, 1H), 4.71 (d, 7=9.8 Hz, 1H), 4.40 (dd, J=\2A, 1.6 Hz, 1H), 4.34 (d, J= 2.8 Hz, 1H), 4.07 (dd, ,7=12.5, 1.5 Hz, H i), 3 85 (s, 3H), 3 56 (d, 7=1 0 Hz, 1H), 3.41 (dd, 7=10 3, 3 3 Hz, ! S i ). 2.19 (s, 3H); LC/MS (ESI) m/e 480.1 [(M+Na) ÷ , calcd for C22H23N30 6 SNa 480.1], fe = 1.83 min (Method 1).

Step 6 Preparation of (2S,4aR,6S,7R,8R,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-ol

To a solution of (2S,4aR,6S,7R,8S,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-i/][1 ,3]dioxin-7-yl acetate (4.2 g, 9 18 mmol) in methanol (6 niL) and THF (42 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (8.40 mL, 36.7 mmol). The reaction mixture was stirred at rt for 1 h. The reaction mixture was cooled to 0 °C and was treated with 1 N HC1 (100 mL).

The mixture was transferred to a separatory funnel and the aqueous layer was extracted with ethyl acetate (3 x 60 mL). The combined organic layers were washed with saturated aqueous NaHCOs (100 mL), brine (100 mL), dried over MgSCL, filtered and concentrated. The solid was then air-dried followed by drying under vacuum to afford (2S,4aR,6S,7R,8R,8aR)-8-azido-2-(4-methoxyphenyl)-6-

(phenylthio)hexahydropyrano[3,2-d][l ,3]dioxin-7-ol (3.81 g, 9 18 mmol, 100% yield) as a white solid. The product was used directly in the next step without further purification. l B NMR (400 MHz, CHLOROFORM-d) d 7 70 (dd, 7=8.0, 1.3 Hz, 2H), 7.40 - 7 30 (m, 5H), 6.92 - 6.87 (m, 2H), 5.52 (s, 1 1 1 ). 4.57 (d, 7=9.3 Hz, 1 1 1). 4.40 (dd, 7=12.4, 1.6 Hz, 1 1 1 ), 4.24 (d, 7=2.8 Hz, H I ), 4.06 (dd, 7=12.4, 1.6 Hz, H I ), 3.99 (t, 7=9.5 Hz, H i), 3.85 (s, 3H), 3.57 (d, 7=1.0 Hz, 1H), 3.49 (dd, 7=10.0, 3.3 Hz, 1H); LC/MS (ESI) m/e 438.1 [ (M Na) , calcd for CioILiNsOsSNa 438.1], fe = 1.69 min (Method 1)

Step 7. Preparation of (2S,4aR,6S,7R,8S,8aR)-8-azido-7-methoxy-2-(4-methoxyphenyl)- 6-(phenylthio)hexahydropyrano[3,2-d][l,3]dioxine

To a solution of (2S,4aR,6S,7R,8R,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-ol (3.8 g, 9.15 mmol) in DMF (75 mL) at 0 °C was added sodium hydride (0.915 g, 22.87 mmol). The cooling hath was removed and the mixture was stirred at room temperature for 30 min. Iodomethane (2.288 mL, 36.6 mmol) was then added and the reaction mixture was stirred for 1 h. The mixture was transferred to a separatory' funnel containing saturated aqueous NaHCC solution (200 mL) and water (200 mL). The aqueous layer was extracted with ethyl acetate (3 x 200 mL). The combined organic layers were washed with brine (250 mL), dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (20% 50% ethyl acetate in hexanes; 330 g column) to afford (2S,4aR,6S,7R,8S,8aR)-8-azido-7-methoxy-2-(4-methoxyphenyl)- 6- (phenylthio)hexahydropyrano[3,2-d][l,3]dioxine (3.7 g, 8.61 mmol, 94% yield) as a white solid. l H NMR (400 MHz, CHLOROFORM-d) d 7.76 - 7.67 (m, 2H), 7.51 - 7.40 (m, 2H), 7.36 - 7.22 (m, 3H), 7.00 - 6.87 (m, 2H), 5.54 (s, 1H), 4.58 (d, ./ 9 3 Hz, 1H), 4.39 (dd, .7=12.5, 1.5 Hz, 1H), 4.24 (d, ,7=2.5 Hz, 1H), 4.05 (dd, .7=12.4, 1.6 Hz, 1H),

3.85 (s, 3H), 3.68 - 3.63 (m, III), 3.62 (s, I I), 3.54 - 3 46 (m, 2H); LC/MS (ESI) m/e 452.1 [(M+Na) ÷ , calcd for CiiHwN OsSNa 452.1], /R = 1.98 min (Method 1).

Step 8. Preparation of (2R,3R,4S,5R,6S)-4-azido-2-(hydroxymethyl)-5-methoxy-0- (phenylthio)tetrahydro-2//-pyran-3-ol

(2S,4aR,6S,7R,8S,8aR)-8-Azido-7-methoxy-2-(4-methoxyphenyl)- 6- (phenylthio)hexahydropyrano[3,2-i/][l,3]dioxine (3.7 g, 8.61 mmol) was dissolved in 80% AcOH (50 mL) and was heated at 40 °C for 2.5 h. The mixture was concentrated and then reconcentrated from heptane (2x). The product was purified by column chromatography on silica gel (10% 75% ethyl acetate in hexanes; 120 g column) to afford (2R,3R,4S,5R,6S)-4-azido-2-(hydroxymethyl)-5-methoxy-6- (phenylthio)tetrahydro-2//-pyran-3-ol (2.6 g, 8.35 mmol, 97% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) 6 7.62 - 7.54 (m, 2H), 7.39 - 7.30 (m, 31 1). 4.66 - 4.61 (m, 1 1 1), 4.06 (t, .7=2.3 Hz, 1 1 1 ).. 4.01 - 3.93 (m, 1 1 1 ), 3.91 - 3.83 (m, 1 1 1 ).. 3.70 (s,

31 1 ), 3.59 - 3.48 (m, 3H), 2.69 (d, .7=3.0 Hz, I I I ). 2.09 (dd, ,7=7.4, 5.4 Hz, 1 H); LC/MS (ESI) m/e 334.0 [(M+Na) + , calcd for CoHnNsCUSNa 334.0], fe = 1.74 min (Method 1).

Step 9. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-4-azido-5-methoxy-6- (phenylthio)tetrahydro-2/: -pyran~2-yl)methyl acetate

To a solution of (2R,3R,4S,5R,6S)-4-azido-2-(hydroxymethyl)-5-methoxy-6- (phenylthio)tetrahydro-2/ -pyran-3-ol (0.580 g, 1.86 mmol) in pyridine (15 mL) was added acetic anhydride (1.055 mL, 1 1.18 mmol) and DMAP (0.023 g, 0.186 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was transferred to a separatory funnel containing ethyl acetete (150 mL). The organic layer was washed with cold 4 N HC1 (2 x 50 mL), saturated aqueous NaHCOr solution (25 mL), and brine (25 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (10% 30% ethyl acetate in hexanes; 40 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-4-azido-5-methoxy-6- (phenylthio)tetrahydro-2i/-pyran-2-yi)methyl acetate (0.735 g, 1 86 mmol, 100% yield) as a colorless oil. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.63 - 7.56 (m, 2H), 7.40 - 7.31 (m, 31 1 ), 5.37 (dd, .7=3.3, 1.0 Hz, 1H), 4 62 (d, 7 9.5 Hz, i l l), 4.12 (d, .7=6.5 Hz, 2H), 3.85 (td, J= 6.5, 1.0 Hz, 1H), 3.69 (s, 3H), 3.60 (dd, .7=9.7, 3.4 Hz, 1H), 3.46 - 3.36

(m. 1 1 1 ), 2.17 (s, 3H), 2.06 (s, 3H); LC/MS (ESI) m!e 418.0 [(M+Na) + , calcd for

CnHiiNsOeSNa 418.1], /R = 2.01 min (Method 1).

Step 10. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-5-methoxy-6-(phenylthio)-4-(4- (3,4,5 -trifl uorophenyl)- 1 H -1,2,3 -triazol - 1 -y Ijtetrahy dro-2/7 -pyran-2-y 1 jmethyl acetate

((2R,3R,4S,5R,6S)-3-Acetoxy-4-azido-5-methoxy-6-(phenylth io)tetrahydro-2/7- pyran-2-yl)methyl acetate (375 mg, 0.948 mmol) and 5-ethynyl-l,2,3-trifluorobenzene (296 mg, 1 897 mmol) were dissolved in a previously degassed solution of DMF (15 mL) and water (5 mL) and the mixture was placed under argon. Sodium ascorbate (188 mg, 0.948 mmol) and copper(II) sulfate pentahydrate (189 mg, 0.759 mmol) (predissolved in

1 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture was filtered through a pad of C elite and the filtrate was transferred to a separatory funnel containing saturated aqueous NaHCCL solution (25 mL). The aqueous layer was extracted with dichloromethane (3 x 50 mL). The combined organic layers were washed with brine (25 mL), dried over MgS() 4 , filtered, and concentrated. The filtrate was concentrated and was purified by column chromatography on silica gel (30% 70% ethyl acetate in hexanes; 40 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy~5- methoxy-6-(phenylthio)-4-(4-(3 ,4, 5-trifl uorophenyl)- 1/7- 1 ,2,3 -triazol- 1 -yDtetrahy dro- 2//~pyran-2-yl)rnethyl acetate (483 mg, 0.876 mmol, 92% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.79 (s, 1H), 7.67 - 7.61 (m, 21 1). 7.46 (dd,

,7=8.3, 6 5 Hz, 21 1 ), 7.40 - 7.35 (m, 31 1 ).. 5.52 (dd, .7=2.9, 0.9 Hz, 1 1 1 ). 4 75 (d, 7=9.5 Hz, 1H), 4.65 (dd, 7=10.2, 3.1 Hz, 1H), 4.37 - 4.29 (m, 1H), 4.21 (qd, .7=1 1.3, 6.4 Hz, 2H), 4.09 - 4.03 (m, 1H), 3.38 (s, 3H), 2.10 (s, 3H), 2.06 (s, 3H); LC/MS (ESI) m/e 574.1 [(M+Na) + , ealed for CLiHirlvsNsOeSNa 574.1], tR = 2, 18 min (Method 1).

Step 1 1

To a solution of ((2R,3R,4S,5R,6S)-3-acetoxy-5-methoxy-6-(phenylthio)-4-(4-

(3,4,5 -trifl uorophenyl)- 1 H -1,2,3 -triazol - 1 -y Ijtetrahy ciro-2// -pyran-2-y 1 jmethyl acetate (250 mg, 0.453 mmol) in CH2CI2 (5 mL) (predried over 4 A molecular sieves) at 0 °C was added bromine (10% stock solution in CH2CI2) (0.467 mL, 0.907 mmol) dropwise. The reaction mixture was stirred at 0 °C for 1 h. Excess bromine was quenched by the addition of saturated aqueous NaHCCh solution and saturated aqueous Na?.S2.03 solution (1 mL:3 mL) (the red color disappeared). The mixture was transferred to a separatory funnel containing water (10 mL). The aqueous layer was extracted with dichloromethane (3 x 15 mL). The combined organic layers were washed with brine (10 mL), dried over Na 2 S04, filtered, and concentrated. The product was purified by column

chromatography on silica gel (20% -- 40% ethyl acetate in hexanes; 24 g column) to afford ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-5-methoxy-4-(4-(3,4,5-tr ifluorophenyl)- l/7-l,2,3-triazol-l-yi)tetrahydro-2//-pyran-2-yi)methyl acetate (195 mg, 0.373 mmol, 82% yield) as a white solid. The product was stored in the freezer until it was used. X H NMR (400 MHz, CHLOROFORM-d) d 7.82 (s, 1H), 7.46 (dd, .7=8 4, 6.4 Hz, 2H), 6.87 (d, .7=3 8 Hz, 1 1 1 ). 5.60 (dd, .7=2.9, 1.4 Hz, 1 1 1), 5.01 (dd, ,7=10.8, 3.0 Hz, 1 1 1), 4.67 -

4.59 (m, 1H), 4.49 (dd, 7=10.8, 3.8 Hz, 1H), 4.25 (dd, 7=11.8, 6.3 Hz, 1H), 4.18 (dd, 7=11.5, 6.8 Hz, H i), 3.40 (s, 3H), 2.1 1 (s, 3H), 2.09 (s, 31 1 ). LC/MS (ESI) mie 522.0 [(M+H) + , calccl for Ci9H2oBrF 3 N3Q6 522.0], ίk = 1.80 min (Method 1). Preparation of (-)-(3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-27/-pyran-4-o l and (+)-

(3S,4R,5S)-3-azido-5-(trityloxy)tetrahydro-2/7-pyran-4-o! Trit ! chloride DMU

Step 1 Step 2

major minor Step 5 major minor racemic "

racemic - relative stereoc emistry relative stereochemistry / ca. 2 5:1 , not separated - mixture carr shown

Step 6B/ SFC chiral / separation

OH " OH OH / OH

(+) (+)

Peak

Peak 1 2 Peak 1 Peak 2 desired enantiomer

desired enantiomer

Step 1. Preparation of l-(allyloxy)but-3-en-2-ol

In a pressure flask, 2-vinyloxirane (7.00 g, 100 mmol) and prop-2-en-l~ol (13.58 mL, 200 mmol) were dissolved in DMF (200 mL) and the solution was cooled to 0 °C. Sodium hydride (7.99 g, 200 mmol) was added to the reaction mixture which was then stirred at 0 °C for 20 min. The pressure flask was then sealed and the reaction mixture was stirred at 50 °C for 16 h. The reaction mixture was cooled to rt and was quenched with 1 N HC1 (200 mL). The mixture was transferred to a separatory funnel and the aqueous layer was extracted with ether (3 x 150 mL). The combined organic layers were washed with brine (200 mL), dried over magnesium sulfate and concentrated under vacuum. The crude product was purified by column chromatography on silica gel (0% 20% ethyl acetate in hexanes; 220 g column) to afford l-(allyloxy)but-3-en-2-ol (10.8 g, 84 mmol, 84% yield) as a colorless oil. ¾ NMR (400MHz, CHLOROFORM-d) 6 5.99 - 5 80 (m, 2FI), 5.43 - 5.19 (m, il l ). 4 38 - 4 32 (m, 1 1 1), 4.06 (dt, .7=5.8, 1.4 FIz, 2H), 3.53 (dd, J=9.7, 3.4 Hz, 1 1 1). 3.36 (del. ,7=9.7, 7.9 Hz, IH), 2.47 - 2.22 (m, 1H). Step 2 Preparation of (((l -(allyloxy)but-3-en-2-yl)oxy)methanetriyl)tribenzene

In a round bottom flask, DBU (45.0 mL. 298 mmol) was added to a solution of 1- (aliy!oxy)but-3-en-2~ol (15.3 g, 1 19 mmol) and (chloromethanetriyl)tribenzene (69.9 g, 251 mmol) in CH2CI2 (250 mL). The reaction mixture was stirred at rt for 2 days. The reaction mixture was concentrated under vacuum and was purified by column chromatography on silica gel (0% 15% ethyl acetate in hexanes; 330 g column, 3 runs) to afford (((l-(allyloxy)but-3-en-2-yl)oxy)methanetriyl)tribenzene (34.2 g, 92 mmol, 77% yield). l B NMR (400MHz, CHLOROFORM-d) d 7.60 - 7.53 (m, 6H), 7 34 - 7 23 (m, 9H), 5.88 - 5.66 (m, 2H), 5 25 - 5 10 (m, 2H), 5.03 - 4.85 (m, 2H), 4 22 - 4.13 (m, 1H), 3.89 - 3.79 (m, 2H), 3.14 - 3.00 (m, 2H); LC/MS (ESI) m/e 393.2 [(M+Na) + , calcd for (ihefEeCLNa 393 2] /R = 1.67 min (Method 1 ).

Step 3 Preparation of 3-(triiyloxy)-3,6-dihydro-2/7-pyran

In a 5 L 3-neck flask, (((l-(allyloxy)but-3-en-2-yl)oxy)methanetriyl)tribenzene (34.2 g, 92 mmol) was added to a solution of (l,3-dimesitylimidazolidin-2-ylidene)(2- isopropoxybenzylidene)ruthenium(VI) chloride (0.578 g, 0.923 mmol) in CH2CI2 (4 L). The reaction mixture was stirred at rt for 16 h. Ethyl vinyl ether (50 mL) was added to the reaction mixture to decompose the catalyst and the reaction mixture was concentrated under vacuum. The crude product was purified by column chromatography on silica gel (0% 10% ethyl acetate in hexanes; 330 g column, 3 runs) to afford 3-(trityloxy)-3,6- dihydro-277-pyran (28.05 g, 82 mmol, 89% yield). Ή NMR (400 MHz,

CHLOROFORM-d) d 7 57 - 7.52 (m, 6H), 7.36 - 7.25 (m, 9H), 5.81 - 5.72 (m, 1H), 5.40 (dq, 7=10.5, 2.6 Hz, 1H), 4.14 - 4.06 (m, 2H), 3.97 - 3.89 (m, 1H), 3.22 (dd, 7=5.1, 1.4 Hz, 2H); LC/MS (ESI) m/e 364.8 | (M Na)\ calcd for CurifeCCNa 365.2] fe = 2.33 min (Method 2)

Step 4 Preparation of (l R,5R,6R)-5-(trityloxy)-3,7-dioxabicyclo[4. l .0]heptane and (I S,5R,6S)-5-(trityioxy)-3,7-dioxabicyclo[4.1 0]heptane

In a round bottom flask under nitrogen, sodium bicarbonate (8.26 g, 98 mmol) was added to a solution of 3-(trityloxy)-3,6-dihydro-2i/-pyran (28.05 g, 82 mmol) in CH2CI2 (950 mL). The reaction mixture was cooled to 0 °C and wrCPBA (36.7 g, 164 mmol) was added to the reaction mixture. The reaction mixture was allowed to slowly warm to rt and w'as stirred for 16 h. The reaction mixture was washed with a saturated aqueous solution of NaiSuCb (400 mL) to destroy excess ?¾CPBA. The organic layer was washed with 1 N NaOH (400 mL) and brine (400 mL), dried over sodium sulfate and concentrated under vacuum. The crude product was purified by column

chromatography on silica gel (0% ® 15% ethyl acetate in hexanes; 330 g column, 3 runs) to afford an inseparable mixture (2.5: 1 ratio) of (lR,5R,6R)-5-(trityloxy)-3,7- dioxabicyclo[4.1.0]heptane (major) and (lS,5R,6S)-5-(trityloxy)-3,7- dioxabicyclo[4.1.0]heptane (minor) (23.8 g, 66.4 mmol, 81% yield) ¾ NMR (400MHz, CHLOROFORMS) d 7.61 - 7.50 (m, 6H), 7.40 - 7.23 (m, 9H), 4.00 (ddd, i=9.3, 5.8,

2.0 Hz, 1H, minor), 3.97 - 3.89 (m, 2H, major), 3.87 - 3.80 (m, 1H), 3.77 - 3.71 (m, 1H, minor), 3 31 (dd, ,7=11.5, 5.3 Hz, 1H, major), 3.27 - 3.21 (m, 2H, minor), 3.05 (dt, ./ 4.0. 0.8 Hz, 1H, major), 3.03 - 2.99 (m, 1H, minor), 2.96 (dd, .7=11.4, 7.9 Hz, 1H, major),

2.70 (d, =4.0 Hz, IH, major), 2.66 - 2 62 (m, 1H, minor);

LC/MS (ESI) m/e 380.8 [(M+Na) + , calcd for C24H 22 03Na 381.2] /R = 2.18 min (Method

1).

Step 5. Preparation of (3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-277-pyran-4-ol and (3 R,4S, 5 R)-3 -azi do-5-(trity 1 oxy)tetrahydro-277-pyran-4-ol

racemic - relative stereochemistry racemic - relative stereochemistry

In a sealed vial, sodium azide (8.60 g, 132 mmol) was added to a solution of a racemic mixture of (lR,5R,6R)-5-(trityloxy)-3,7~dioxabicycio[4.1 0]heptane and

(lS,5R,6S)-5-(trityloxy)-3,7-dioxabicyclo[4. l.0]heptane (23.7 g, 66.1 mmol) in MeOH (500 mL) and water (55 6 L). The reaction mixture was stirred for 16 h at 80 °C. The reaction mixture was partitioned between water (300 mL) and CH2CJ2 (300 mL). The aqueous layer was washed with CH2CI2 (3 x 200 mL). The combined organic layers were dried over sodium sulfate and concentrated under vacuum. The product was purified by column chromatography on silica gel (0% -- > 35% ethyl acetate in hexanes; 330 g column, 3 runs) to afford the products (3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro- 2i/-pyran-4-ol (9.30 g, 23.17 mmol, 35% yield) and (3R,4S,5R)-3-azido-5- (trityloxy)tetrahydro-2/7-pyran-4-ol (5.30 g, 13.20 mmol, 20% yield).

Data for (3S,4R,5R)-3-azido-5-(trityioxy)tetrahydro-2/7-pyran-4-ol (Compound

A): TI NMR (400 MHz, CHLOROFORM-d) d 7.55 - 7.45 (m, 61 1 ). 7 40 - 7.24 (m, 9H), 3.87 - 3.76 (m, 21 1). 3.41 - 3.22 (m, 3H), 3.17 - 2.97 (m, 21 1). 2.37 (s, 1 1 1); LC/MS (ESI) m/e 423.7 [(M+Na) ÷ , calcd for C24H23N303Na 424.2] /R = 2.18 min (Method 2).

Data for (3R,4S,5R)-3-azido-5-(trityloxy)tetrahydro-2//-pyran-4-ol (Compound

B): ¾ NMR (400 MHz, CHLOROFORM-d) d 7.56 - 7.48 (m, 6H), 7.41 - 7.25 (m, 9H), 3.96 - 3 80 (m, 21 1), 3.68 - 3.55 (m, 21 1), 3 45 (td, ./ 1 1 2, 4.1 Hz, 21 1 ). 2.95 - 2.75 (m, 1H), 2.46 (d, 7=3.3 Hz, i l l): LC/MS (ESI) m/e 423.7 [(M+Na) + , calcd for C’d HX TLXa

424.2] tR = 2.17 min (Method 2).

Step 6A. Preparation of (-)-(3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-2/ -pyran-4-ol

relative stereochemistry P ea k 1 (undesired) 2 (desired)

A racemic mixture of compound A (9.3 g) from above was separated by preparative SFC chiral chromatography using the conditions described below:

Preparative chiral HPLC conditions:

Preparative Column: OJ-H (5x25 cm, 5 pm, #74123)

BPR pressure: 100 bars

Temperature: 35 °C

Flow rate: 300 inL/mit

Mobile Phase: CC / MeOH (90/10)

Detector Wavelength 220 ran

Separation Program: stack injection

Injection: 0.6 mL with cycle time: 2 75 mins

Sample preparation: 9.3 g / 1 50ml . MeOH:DCM (4: 1), 62 mg/mL

Throughput: 81 1 rng/h Subsequent analysis of the individual enantiomers by chiral HPLC was conducted using the conditions below:

Analytical chiral HPLC conditions:

Analytical Column: QJ-H (0.46x25 cm, 5 mhi)

BPR pressure: 140 bars

Temperature: 40 °C

Flow rate: 3.0 L/min

Mobile Phase: CO2/ MeOH (90/10)

Detector Wavelength: UV 200-400 nil!

Peak 1 (undesired): (+)-(3R,4S,5S)-3-azido-5-(trityloxy)tetrahydro-2 -pyran-4-ol (3.8 g): chiral HPLC ΐk = 5.44 min; [<X]D 22 +11.5, (c = 0.755, MeOH).

Peak 2 (desired): (-)-(3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-2 -pyran-4-ol (3.6 g): chiral HPLC fe = 6.06 min; [of 22 -11.9, (c = 0.575, MeOH).

Step 6B. Preparation of (+)-(3S,4R,5S)-3-azido-5-(trityloxy)tetrahydro-2/ -pyran-4-ol

relative stereochemistry Peak 1 (desired) Peak 2 (undesired)

A racemic mixture of compound B (5.3 g) from above was separated by- preparative SFC chiral chromatography using the conditions described below:

Preparative chiral HPLC conditions:

Preparative Column: OJ-H (5x25 cm, 5 pm, #74123)

BPR pressure : 100 bars

Temperature: 35 °C Flow rate: 300 mL/min

Mobile Phase: C() 2 / MeOH (70/30)

Detector Wavelength: 220 nm

Separation Program: stack injection

Injection: 0.5mL with cycle time: 1.75 mins

Sample preparation: 5.3 g / 56 ml. MeOH:DCM (1 : 1), 94 64 rng/mL

Throughput: 1.6 g/h

Subsequent analysis of the individual enantiomers by chiral HPLC was conducted usin the conditions below:

Analytical Conditions:

Analytical Column: OJ-H (0.46x25 cm, 5 pm)

BPR pressure : 140 bars

Temperature: 40 °C

Flow' rate: 3.0 rnL/min

Mobile Phase: CO2. / MeOH (90/10)

Detector Wavelength: UV 200-400nm Peak 1 (desired): (+)-(3S,4R,5S)-3-azido-5-(trityloxy)tetrahydro-2i/-pyran-4-o l

(2.6 g): chiral HPLC fe = 4.81 min; [a jo " 32 1 , (c = 0.575, MeOH).

Peak 2 (undesired): (-)-(3R,4S,5R)-3-azido-5-(trityloxy)tetrahydro-2/7-pyran-4- ol (2.41 g): chiral HPLC ΐk = 6.89 min; [ajo 22 -29.4, (c = 0.575, MeOH).

Preparation of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//-pyran-4-ol (Method 1)

OH ? Ac

Ac % pyridine formic acid H O «V^ ^ N 3 X T Et 2 0 ^CT

Step 1 Step 2 (-)

OAc

1) q, pyridine, CH 2 C! 2 1) q, pyridine, CH 2 Ci 2

2) letrabulylammonium nitr i te 2) potassium thioacetate,

DtVIF acetone

Slep 3 Step 4

NaOMe in MeOH

THF

Step 5

OH OAc OH hydrazine hydrate . N, HS * . HS .A. N 3 HOAf: THF

"O'

Step 5 (-)

(Alternate conditions) (-)

Step 1. Preparation of (-)-(3S,4R,5R)-3~Azido-5-(triiyloxy)ietrahydro-2/:/-pyran-4- yl acetate

Acetyl chloride (1.240 mL, 17 44 mmol) was added to a solution of (-)- (3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-2//-pyran-4-ol (3.50 g, 8 72 mmol) and pyridine (2.115 mL, 26 2 mmol) in CH2CI2 (100 mL) under nitrogen. The reaction mixture was stirred at rt for 16 h. The reaction mixture was diluted with CH2CI2 (20 mL) and the organic layer was washed with IN HC1 (50 mL), sat. sodium bicarbonate (50 mL), and brine (50 mL). The organic layer was dried over MgSOr, filtered, and concentrated under vacuum to afford (-)-(3S,4R,5R)-3-azido-5-(trityloxy)tetrahydro-2i7- pyran-4-yl acetate (3.73 g, 8.41 mmol, 96% yield). The product was used in the next step without further purification. [CX]D” -8.8, (c = 0.340, CHCb); Ή NMR (400 MHz, CHLOROFORM-d) d 7.55 - 7.46 (m, 6H), 7.38 - 7.22 (m, 9H), 5.34 (t, J=9.2 Hz, 11 1). 3.85 (dd, ./ i 1 .4. 5.1 Hz, H i), 3.58 - 3 44 (m, H I), 3.42 - 3.32 (m, 1H), 3.30 - 3 10 (m, 31 !), 2.03 (s, 31 !). LC/MS (ESI) m/e 465 7 [(M+Na) + , calcd for C26H25N3O4 466 2], /i < = 2.31 min (Method 2). Step 2. Preparation of (-)-(3S,4R,5R)-3-Azido-5-hydroxytetrahydro-2i/-pyran-4-yl acetate

(-)-(3S,4R,5R)-3-Azido-5-(trityloxy)tetrahydro-2i/-pyran-4-y l acetate (1.6 g,

3.61 mmol) was added to a solution of formic acid (4 mL, 104 mmol) and Et20 (6 mL). The reaction mixture was stirred overnight at rt. The reaction progress was monitored by TLC (Hanessian's stain). The mixture was concentrated under vacuum and purified by- column chromatography on silica gel (20% 70% ethyl acetate in hexanes; 80 g column) to afford (-)-(3S,4R,5R)-3-azido-5-hydroxytetrahydro-2i/-pyran-4-yl acetate (570 mg, 2.83 mmol, 79% yield) [of 22 -58.4, (c = 0 290, CHCI3); ' l l NMR (400 MHz, CHLOROFORM-d) d 4 84 (t, ./ 8 9 Hz, 1 H), 4.05 - 3.93 (m, 2H), 3 73 - 3 64 (rn, 1H), 3.63 - 3.53 (m, 1H), 3.21 (ddd, J=11.7, 10.0, 8.7 Hz, 2H), 2.18 (s, 3H); 13 C MR (101 MHz, CHLOROFORM-d) 6 171.7, 77.9, 70 2, 68 8, 67 8, 58 6, 20.6.

Step 3 Preparation of (+)-(3S,4R,5S)-3-Azido-5-hydroxytetrahydro-2//-pyran-4-yl acetate

Trifiuorornethanesulfonic anhydride (0 846 mL, 5.01 mmol) was added to a solution of (-)-(3S,4R,5R)-3-azido-5-hydroxytetrahydro-2i/-pyran-4-yl acetate (0.840 g, 4.18 mmol) and pyridine (1.013 mL, 12.53 mmol) in CH2CI2 (40 mL) at -10 °C. The reaction mixture was stirred at -10 °C for 1.5 h. The reaction progress was monitored by TLC (Hanessian's stain). The reaction mixture was diluted with CH2G2 (20 mL) and was washed with 1 N HC1 (30 mL), sat. sodium bicarbonate (30 nil.) and brine (30 mL). The organic layer was dried over MgSCri, filtered, and concentrated under vacuum to afford (3S,4R,5R)-3-azido-5-(((trifluoromethyl)sulfonyl)oxy)tetrahy dro-2//-pyran-4-yl acetate (1.39 g, 4.18 mmol, 100% yield). The product was used without further purification.

In a pressure vessel, tetrabutyl ammonium nitrite (3 62 g, 12.54 mmol) was added to a solution of crude (3S,4R,5R)-3-azido-5-(((trifluoromethyl)sulfonyl)oxy)tetrahy dro- 2/ -pyran-4-yl acetate (1.39 g, 4.18 mmol) from above in DMF (40 mL). The vessel was sealed and the reaction mixture was heated at 50 °C for 1.5 h. The reaction progress was monitored by TLC (Hanessian's stain). The reaction mixture was cooled to rt and concentrated under vacuum. The product was purified by column chromatography on silica gel (10% -----> 70% ethyl acetate in hexanes; 80 g column) to afford (+)~(3S,4R,5S)~ 3-azido-5-hydroxytetrahydro-2//-pyran-4-yl acetate (0.46 g, 2.267 mmol, 54% yield) l a in” +34.7, (c = 0.325, CHCb); l NMR (400 MHz, CHLOROFORM-d) 6 4.88 (dd, ./ 9.4. 3 1 Hz, 1 1 1 ), 4. 13 - 4.09 (m, ! H), 4.07 - 3.91 (m, 31 1 ), 3.56 (dd, ./ 1 2.4, 1.6 Hz, 1H), 3.22 (dd, J=l 1.3, 9.8 Hz, 1H), 2.43 (br s, 1H), 2.20 (s, 3H); l3 C NMR (101 MHz, CHLOROFORM-d) d 169.8, 74.5, 69.8, 67.9, 66.5, 56.1 , 20.7.

Step 4. Preparation of (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydro-2/:/-pyran~4 ~yl acetate

To a solution of (+)-(3 S,4R,5S)-3-azido-5-hydroxytetrahydro-2//-pyran-4-yl acetate (1.95 g, 9.69 mmol) and pyridine (3.92 niL, 48.5 mmol ) in CH2CI2 (50 mL) at -10 °C was added trifluoromethansulfonic anhydride (4.09 mL, 24.23 mmol) dropwise. The reaction mixture was stirred at -10 °C for 30 min. The reaction progress was monitored by TLC (Hanessian's stain). The mixture was transferred to a separator}' funnel containing dichloromethane (25 mL) and was washed with cold 1 N HC1 (25 mL), saturated aqueous NaHCOs (50 mL), brine (50 mL), dried over Na2S0 4 , filtered, and concentrated. The crude product was then dissolved in dry acetone (50 mL) (predried over 4 A molecular sieves) and the mixture was cooled to -10 °C. Potassium thioacetate (5.53 g, 48.5 mmol) was added and the reaction mixture was stirred at -10 °C for 1.5 h. The reaction progress was monitored by TLC (Hanessian's stain). The mixture was transferred to a separatory' funnel containing water (40 mL). The aqueous layer was extracted with dichloromethane (3 x 40 mL). The combined organic layers were washed with brine (40 mL), dried over MgSCL, filtered, and concentrated. The product was purified by column chromatography on silica gel (0% --> 40% ethyl acetate in hexanes; 120 g column) to afford (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydro-2i/-pyran-4- yl acetate (1.92 g, 7 41 mmol, 76% yield) [of 22 +12 8, (c = 0.510, CHCb); Ή NMR (400 MHz, CHLOROFORM-d) 6 4 98 (dd, ./ 10.8, 9.3 Hz, i l l ). 4 08 - 4.01 (m, 1 1 1), 3.95 (ddd, =11.5, 5.0, 0.9 Hz, 1H), 3.71 - 3.61 (m, 21 1). 3.30 (t, =11.3 Hz, 1H), 3.24 - 3.15 (m, 1H), 2.32 (s, 3H), 2.11 (s, 3H); 13 C NMR (101 MHz, CHLOROFORM-d) d 193.3, 169 8, 72.1, 69.4, 68.2, 60.4, 43.0, 30.4, 20.4. Step 5. Preparation of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//-pyran-4-ol

To a solution of (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydro-2//-pyran-4- yl acetate (1.92 g, 7.41 mmol) in THF (100 mL) at -10 °C was added sodium methoxide (25% in MeOH) (8.47 mL, 37.0 mmol) dropwise via syringe. The reaction mixture was stirred at -10 °C for 15 min. The reaction progress was monitored by TLC (Hanessian's stain). The reaction mixture was transferred to a separator} ' funnel containing saturated aqueous MHO solution (50 mL). The aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed with brine (50 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (0% --> 50% ethyl acetate in hexanes; 40 g column) to afford (-)-

(3S,4R,5R)-3-azido-5-mercaptotetrahydro-27/-pyran-4-ol (0.900 g, 5.14 mmol, 69% yield) [ajo 22 -107.5, (c = 0.255, CHCb); ¾ NMR (400 MHz, CHLOROFORM-d) d 4.06 (td, ,7=11.3, 5.0 Hz, 2H), 3.53 (ddd, ,7=10.8, 9.3, 5.0 Hz, 1H), 3.38 - 3.29 (m, 1H), 3.18 (t, 7=11.5 Hz, 2H), 2.96 (d, .7=1 8 Hz, 1 1 1), 2.90 - 2.76 (m, H i).. 1.32 (d, 7 10.0 Hz, i l l); °C NMR (126 MHz, CHLOROFORM-d) 6 78.8, 72.7, 69.0, 62.3, 42.6.

Step 5 (Alternate conditions). Preparation of (-)-(3S,4R,5R)-3-azido-5- mercaptotetrahydro-2i7-pyran-4-ol and (-)-(3 S,4R,5R)-3-azido-5-mercaptotetrahydro- 2/ -pyran-4-yl acetate

To a solution of (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydn>27/-pyran- 4-yl acetate (135 mg, 0.521 mmol) in THF (8 mL) at -78 °C was added hydrazine hydrate (0.126 mL, 2.60 mmol) dropwise via syringe. The cooling bath was then replaced with an ice-water bath and the reaction mixture was stirred at 0 °C for 15 min. Acetic acid (0.149 mL, 2.60 mmol) was then added and stirring was continued for 5 min. The mixture was transferred to a separator} ' funnel containing water (10 mL). The aqueous layer was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (10 mL), dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (10% ---> 50% ethyl acetate in hexanes; 24 g column) to afford (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//- pyran-4-ol (29.2 mg, 0 167 mmol, 32% yield) and (-)-(3S,4R,5R)-3-azido-5- mercaptotetrahydro-2//-pyran~4-yl acetate (31 mg, 0.143 mmol, 27% yield).

Data for (-)~(3 S,4R, 5R)-3 -azi do-5-mercaptotetrahy dro-2 -py ran-4~ol ;

[a]o 22 -107.51, (c = 0.26, CHCh); Ή NMR (500 MHz, CHLOROFORM-d) d 7.30 - 7.27 (m, 1H), 4.10 - 4.00 (m, 2H), 3.58 - 3.46 (m, 1H), 3.33 (br t, J=9.5 Hz, 1H), 3.22 - 3.13 (m, 2H), 2.98 (br s, i l l ). 2 82 (ddt, ./ 8.4. 3 2, 1 6 Hz, 1 1 1), 1.33 (dd, 7=9.8, 1.3 Hz, 1 1 1 ); ! 3 C NMR (126 MHz, CHLOROFORM-d) 6 78.8, 72.7, 69.0, 62.3, 42.6.

Data for (-)-(3 S,4R,5R)-3-azido-5-mercaptotetrahydro-2//~pyran-4-yl acetate: [CC]D 22 -113.4, (c = 0.290, CHCh); ¾ NMR (500 MHz, CHLOROFORM-d) d 4.91 - 4.82 (m, 1 H), 4.11 - 4.03 (m, 2H), 3.57 (ddd, 7=40.9, 9.6, 5.2 Hz, 1H), 3.24 (td, 7=1 1.4, 9.7 Hz, 2H), 2.88 (qd, ,7=10.5, 4.7 Hz, 1H), 2.21 (s, 3H), 1.35 (d, 7=9.8 Hz, 1H); 13 C NMR (126 MHz, METH AN OL-d 4 ) d 170.6, 77 5, 72 6, 68.1 , 60 8, 39.3, 19.4

Preparation of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//-pyran-4-ol (Method 2

1 ) * ¾0, pyridine, CH 2 CI 2 NaOMe in MeOH

2) potassium thioacetate, THF

acetone

Step 4

Step 3

Step 1. Preparation of (+)-(3S,4R,5S)-3-azido-5-(trityloxy)tetrahydro-2i/-pyran-4-y l acetate

In a round bottom flask, acetyl chloride (1.417 mL, 19 93 mmol ) was added to a solution of i+)-(3S,4R,5S)-3-azido-5-(trityioxy)tetrahydro-2i/-pyran-4-o l (4.0 g, 9.96 mmol) and pyridine (2.418 mL, 29.9 mmol) in CH2CI2 (100 mL) under nitrogen. The reaction mixture was stirred at rt for 18 h. The reaction mixture was diluted with CH2CI2 (20 mL) and the organic layer was washed with IN HC1 (50 mL), saturated aqueous NaHCOs solution (50 mL), and brine (50 mL). The organic layer was dried over MgSCri, filtered, and concentrated under vacuum to afford (+)-(3S,4R,5S)-3-azido-5- (trityloxy)tetrahydro-277-pyran-4-yl acetate (4.42 g, 9.96 mmol, 100% yield). The product was used in the next step without further purification. [a]o 22 +56.6, (c = 0 450, CHCh); ¾ NMR (400 MHz, CHLOROFORM-d) d 7.55 - 7.48 (m, 6H), 7.38 - 7 25 (m, 9H), 4.62 (dd, .7=7.5, 3.3 Hz, 1H), 4 07 - 3.83 (m, 3H), 3.43 - 3.22 (m, 2H), 3.02 (dd, .7=12.0, 2.8 Hz, H I), 2.25 (s, 3H); LC/MS (ESI) m/e 465.7 [(M+Na) + , calcd for

C26H25N3Q4 466.2], /R ::: 2 36 min (Method 2)

Step 2 Preparation of (+)-(3S,4R,5S)-3-azido-5-hydroxytetrahydro-2//-pyran-4-yl acetate

In a round bottom flask, (+)-(3S,4R,5S)-3-azido-5-(trityloxy)tetrahydro-2/7- pyran-4-yl acetate (4.42 g, 9.97 mmol) in formic acid (19 mL, 495 mmol) and EtiO (30 mL) was stirred at rt for 16 h. The mixture was concentrated under vacuum and the crude product was purified by column chromatography on silica gel (0% ----> 60% ethyl acetate in hexanes; 40 g column) to afford (+)-(3S,4R,5S)-3-azido-5-hydroxytetrahydro- 2//~pyran-4-yl acetate (1.95 g, 9.69 mmol, 97% yield) [of 22 +35.1, (c = 0.360, CHCb); 1 H NMR (400 MHz, CHLOROFORM-d) d 4 90 (dd, .7=9.4, 3.1 Hz, 1 1 1 ), 4.14 - 3 90 (m, 4H), 3.58 (dd, .7=12.4, 1.6 Hz, 1H), 3.23 (dd, .7=1 1.3, 9.8 Hz, 1H), 2.22 (s, 3H); °C NMR (101 MHz, CHLOROFORM-d) d 169 8, 74 5, 69 8, 67 9, 66.5, 56.1, 20.7

Step 3 Preparation of (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydro-2//-pyran-4- yl acetate

To a solution of (+)-(3 S,4R,5S)-3-azido-5-hydroxytetrahydro-2i : /-pyran-4-yl acetate (1.95 g, 9.69 mmol) and pyridine (3.92 mL, 48.5 mmol) in CH2CI2 (50 mL) at -10 °C was added trifluoromethansulfonic anhydride (4.09 mL, 24.23 mmol) dropwise. The reaction mixture was stirred at -10 °C for 30 min. The reaction progress was monitored by TLC (Hanessiaris stain). The mixture was transferred to a separatory funnel containing dichloromethane (25 mL) and was washed with cold 1 N HC1 (25 mL), saturated aqueous NaHCCh (50 mL), brine (50 mL), dried over NarSCri, filtered, and concentrated. The crude product was then dissolved in dry acetone (50 mL) (predried over 4 A molecular sieves) and the mixture was cooled to -10 °C. Potassium thioacetate (5.53 g, 48.5 mmol) was added and the reaction mixture was stirred at -10 °C for 1.5 h. The reaction progress was monitored by TLC (Hanessian's stain). The mixture was transferred to a separatory funnel containing water (40 mL). The aqueous layer was extracted with dichloromethane (3 x 40 mL). The combined organic layers were washed with brine (40 mL), dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (0% 40% ethyl acetate in hexanes; 120 g column) to afford (+)-(3R,4R,5S)-3-(acetylthio)-5-azidotetrahydro-2/7-pyran-4- yl acetate (1.92 g, 7.41 mmol, 76% yield). [OC]D 22 +12.8, (c = 0.510, CHCb); Ή NMR (400 MI L, CHLOROFORM-d) 6 4.98 (dd, .7=10.8, 9.3 Hz, 1 1 1). 4.08 - 4.01 (m, 1H), 3.95 (ddd, 7=1 1.5, 5.0, 0.9 Hz, i l l ), 3.71 - 3 61 (m, 21 1 ), 3.30 (t, 7=1 1.3 Hz, i l l ). 3 24 - 3 15 (m, 1 H), 2.32 (s, 3H), 2.11 (s, 3H); 13 C NMR (101 MHz, CHLOROFORM-d) d 193 3, 169.8, 72.1, 69.4, 68.2, 60.4, 43.0, 30.4, 20.4.

Step 4

To a solution of (+)~(3R,4R,5S)-3~(aeetylthio)-5~azidotetrahydro-2//~pyran-4- yl acetate (1.92 g, 7.41 mmol) in THF (100 ml .) at -10 °C was added sodium methoxide (25% in MeOH) (8.47 mL, 37.0 mmol) dropwise via syringe. The reaction mixture was stirred at -10 °C for 15 min. The reaction progress was monitored by TLC (Hanessian's stain). The reaction mixture was transferred to a separator}' funnel containing saturated aqueous NFbCl solution (50 mL). The aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic layers rvere washed with brine (50 mL), dried over MgSCfi, filtered, and concentrated. The product was purified by column chromatography on silica gel (0% 50% ethyl acetate in hexanes; 40 g column) to afford (--)- (3S,4R,5R)~3-azido-5~mercaptoietrahydro~2/7-pyran~4-ol (0.900 g, 5.14 mmol, 69% yield) [o 22 -107.5, (c = 0.255, CHCb); Ή NMR (400 MHz, CHLOROFORM-d) d 4.06 (td, 7=11.3, 5.0 Hz, 21 1). 3.53 (ddd, 7=10.8, 9.3, 5.0 Hz, 1 1 1). 3.38 - 3.29 (m, 1H), 3.18 (t, 7=11.5 Hz, 2H), 2.96 (d, 7=1 8 Hz, Hi), 2.90 - 2.76 (m, i l l ).. 1.32 (d, 7=10.0 Hz, 1 1 1 ); 13 C NMR (126 MHz, CHLOROFORM-d) 6 78.8, 72.7, 69.0, 62.3, 42.6.

Preparation of (+)-((2R,3R,4S,5R,6S)-3-acetoxy~6-(((3R,4R,5 S)-5-azido-4- hydroxytetrahydro-27 -pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-li/ - 1 ,2,3 -tri azol- 1 -yl)tetrahydro-27/-pyran-2-yl)m ethyl acetate

To a solution of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2/7-pyran-4-ol (225 mg, 1 284 mmol) in ethyl acetate (10 ml.) and sodium carbonate solution (1 M, aq) (5 14 mL, 5.14 mmol) was added ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-5-methoxy-4- (4-(3,4,5-trifluorophenyl)-l//~l,2,3-triazol-l ~yl)tetrahydro-2ii-pyran-2-yl)methyl acetate (838 nig, 1.605 mmol) in ethyl acetate (4 mL) via cannula. Tetrabutyl ammonium hydrogen sulfate (1744 mg, 5.14 mmol) was added and the reaction mixture was stirred vigorously at room temperature for 2.5 h. The mixture was transferred to a separatory tunnel containing saturated aqueous NaHCCb solution (20 mL). The aqueous layer was extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with brine (25 mL), dried over MgSOy filtered, and concentrated. The product was purified by column chromatography on silica gel (15% -- 80% ethyl acetate in hexanes; 40 g column) to afford (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5 -trifluorophenyl)-li/- l ,2,3-triazol-l-yl)tetrahydro-2f/-pyran-2-yl)methyl acetate (670 mg, 1 09 mmol, 85% yield) as a white solid. [<X]D 22 +37.99, (c = 0.240, CHCb); ¾ NMR (500 MHz,

CHLOROF ORM-d) d 7.81 (s, 1H), 7.47 (dd, J=8.2, 6.5 Hz, 2H), 5.52 (d, J= 2.9 Hz, 1H), 4.66 - 4.59 (m, 2H), 4 28 - 4 19 (m, 2H), 4.18 - 4.09 (m, 3H), 4 05 - 3.96 (m, 2H), 3.67 - 3.60 (m, 1H), 3.54 (ddd, J=10.9, 9.1, 5.2 Hz, 1H), 3.35 (s, 3H), 3.26 (t, J=\ 1.9 Hz, 1H), 3.13 (t, ./ 1 1 .3 Hz, H I ), 2.99 (ddd, ./ 1 1 9, 9.7, 5.1 Hz, H I ), 2.15 (s, 3H), 2.12 (s, 3H); LC/MS (ESI) m/e 617.2 [(M+H) + , calcd for C24H28F3N&O8S 617.1], fe = 2.03 min (Method 1).

Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4

hydroxytetrahydro-2/7-pyran-3-yl)†hio)-4-(4-(3-fluoroph enyl)-l /7-I ,2,3-triazol-l-yl)-5- methoxytetrahydro-2/ -pyran-2-yl)methyl acetate

Step 3

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)-li7-l,2,3- triazol- 1 -yl)-5 -m ethoxy-6-(phenylthi ojtetrahy dro-2/ -pyran-2-y 1 )m ethyl acetate

((2R,3R,4S,5R,6S)-3-Acetoxy-4-azido-5-methoxy-6-(phenylthio) tetrahydro-2i7- pyran-2-yl)methyl acetate (70 mg, 0.177 mmol) and l-ethynyl-3-fluorobenzene (63.8 mg, 0.531 mmol) were dissolved in a previously degassed solution of DMF (1.5 mL) and water (0.500 mL) and the mixture was placed under argon. Sodium ascorbate (35.1 mg, 0.177 mmol) and copper(II) sulfate pentahydrate (30.9 mg, 0 124 mmol) (predissolved in 0.4 mL water) were added and the reaction mixture was stirred at room temperature for

14 h. The mixture was filtered through a pad of Celite and the filtrate was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (10 mL). The aqueous layer was extracted with dichloromethane (3 x 15 mL). The combined organic layers were washed with brine (10 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (30% ----> 80% ethyl acetate in hexanes; 24 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3- fluorophenyl)- IHA ,2, 3 -triazol- 1 -yl)-5-methoxy-6-(phenylthio)tetrahydro-27/-pyran-2- yl)methyl acetate (76 mg, 0.147 mmol, 83% yield) as a white solid fit NMR (400 MHz, CHLOROF ORM-d) d 7.84 (s, 1H), 7.68 - 7.62 (m, 2H), 7.62 - 7.53 (m, 2H), 7.45 - 7.33 (m, 4H), 7.05 (tdd, ./ 8.4.. 2.5, 0.8 Hz, 1 1 1), 5.57 - 5.51 (m, H I), 4.76 (d, .7=9.5 Hz, i l l). 4.69 (dd, .7=10.2, 3.1 Hz, 1H), 4.31 (t, ,1=9.9 Hz, 1H), 4.26 - 4.14 (m, 2H), 4.10 - 4.02 (m, 1 1 1 ), 3.37 (s, 3H), 2 10 (s, 3! ! }. 2 06 (s, 3H); LC/MS (ESI) m/e 515.7 [(M+H) + , calcd for C25H276FN3O6S 516.2], tR = 1.99 min (Method 2). Step 2. Preparation of ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-4-(4-(3-fluorophenyl)-lZ - l,2,3-triazol-l-yl)-5-methoxytetrahydro-27/-pyran-2-yl)methy l acetate

To a solution of ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)- 177-1, 2,3- triazol-l-yl)-5-methoxy-6-(phenylthio)tetrahydro-27/-pyran-2 -yl)methyl acetate (75 mg, 0.145 mniol) in CH2CI2 (2 mL) (predried over 4 A molecular sieves) at 0 °C was added Bn (10% in DCM) (0.150 mL, 0.291 mmol) dropwise. The reaction mixture was stirred at 0 °C for 2 h. The crude product was purified by column chromatography on silica gel (0% 50% ethyl acetate in hexanes; 12 g column) to afford ((2R,3R,4S,5R,6R)-3- acetoxy-6-bromo-4~(4-(3 -fluorophenyl)- \H~ 1,2,3 -triazol- 1 -yl)-5~methoxytetrahydro-2/ ~ pyran-2-yl)methyi acetate (55 mg, 0.113 mmol, 78% yield). ! H NMR (400 MHz, CHLOROFORM-d) d 7 85 (s, 1 1 1 ). 7 63 - 7.54 (m, 2H), 7.45 - 7.36 (m, 1 1 1 ).. 7.10 - 7.01 (m. 1 1 1 ), 6.87 (d, ,/ 3.8 Hz, i l l ), 5 62 (dd, 7=3.0, 1.3 Hz, H i ), 5.02 (dd, 7=10.8, 3 0 Hz, 1H), 4.64 (td, 7=6.5, 0.9 Hz, 1H), 4.49 (dd, 7=10.8, 3.8 Hz, 1H), 4.29 - 4.11 (m, 2H),

3.39 (s, 3H), 2.1 1 (s, 3H), 2.09 (s, 3H); LC/MS (ESI) mie 485.6 [(M+H) + , calcd for C 19H2.2B rFN 3 Oό 486.1], i\< = 1.76 min (Method 2).

Step 3

To a solution of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-27 -pyran-4-ol (15 mg, 0.086 mmol) in ethyl acetate (1 mL) was added 2 M sodium carbonate (0.171 mL, 0.342 mmol), ((2R,3R,4S,5R,6R)-3-acetoxy~6-bromo-4-(4~(3-fiuorophenyl)-l/ 7-l,2,3~ triazol-l -yl)-5-methoxytetrahydro-2/7-pyran-2-yl)methyl acetate (52.0 mg, 0.107 mmol), and tetrabutyl ammonium hydrogen sulfate (116 mg, 0.342 mmol). The reaction mixture was stirred vigorously at room temperature for 1 5 h. The mixture was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (20 mL). The aqueous layer was extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with brine (25 mL), dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (15% - 80% ethyl acetate in hexanes; 24 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4- hydroxytetrahydro-27/-pyran-3-yl)thio)-4-(4-(3-fluorophenyl) -I7/-l,2,3-triazol-l-yi)-5- methoxytetrahydro-27/-pyran-2-yl):methyl acetate (44 mg, 0 076 mmol, 89% yield) 1H NMR (400 MHz, CHLOROFORM-d) d 7.86 (s, 1H), 7.63 - 7.54 (m, 2H), 7.42 (td,

J= 7 9, 6 0 Hz, 1 1 1 ), 7.07 (td, ./ 8.4, 2.5 Hz, 1 1 1 ).. 5.54 (d, .7=3.0 Hz, 1H), 4 68 (dd, 7=10.3, 3.0 Hz, 1H), 4.65 (d, =9.8 Hz, 1H), 4.27 - 4.19 (m, 2H), 4.18 - 4.12 (m, 3H),

4.00 (dd, ./ 1 1 .5, 5.3 Hz, Hi), 3.67 - 3.60 (m, H i ), 3.54 (td, ./ 10 0, 5.1 Hz, H I ), 3.34 (s, 3H), 3.26 (t, .7=11.8 Hz, 1H), 3.13 (t, J=l 1.3 Hz, 1H), 3.00 (ddd, ,/=! 1.9, 9.7, 5.0 Hz,

1H), 2.15 (s, 3H), 2.13 (s, 3H); LC/MS (ESI) m/e 581.1 l(M 1 I V, calcd for

C24H30FN6O8S 581.2], (\ > = 1 77 min (Method 2)

Preparation of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyi)-2-

(((3R,4R,5S)-5-azido-4-hydroxytetrahydro-2i7-pyran-3-yl)t hio)-4-(4-(3,4,5- trifluorophenyl)~l/7-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yi)oxy)acetate

Step 1. Preparation of methyl 2-(((4aR,6S,7R,8S,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3 ,2-d] [ 1 , 3 ]dioxin-7-yl)oxy)acetate

To a solution of (4aR,6S,7R,8R,8aR)-8-azido-2-(4-methoxyphenyl)-6- (phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-ol (1.59 g, 3.83 mmol) in DMF (30 niL) was added sodium hydride (0.459 g, 11.48 mmol). After Stirling for 15 min, the reaction mixture was cooled to 0 °C and methyl 2-bromoacetate (1.449 mL, 15.31 mmol) was added via syringe. The reaction mixture was then stirred at rt for 1.5 h. The reaction mixture was cooled to 0 °C and was quenched by the addition of saturated aqueous NaHCCb solution (5 mL) and water (5 mL). The mixture was transferred to a separatory funnel containing ether (300 mL). The organic layer was washed with water (4 x 50 mL) and brine (25 mL), dried over MgSCri, filtered, and concentrated. The product w ? as purified by column chromatography on silica gel (30% -- 60% ethyl acetate in hexanes; 120 g column) to afford methyl 2-(((4aR,6S,7R,8S,8aR)-8-azido-2-(4-methoxyphenyl)- 6-(phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7-yl)oxy)ace tate (1.53 g, 3.14 mmol, 82% yield) as a white solid l B NMR (400 MHz, CHLOROFORM-d) d 7.73 - 7.66 (m, 2H), 7.46 - 7.40 (m, 2H), 7.32 - 7.20 (m, 3H), 6.96 - 6.90 (m, 2H), 5.55 (s, 1H), 4.69 (d,

./ 9.3 Hz, 1H), 4.51 (d, .>45 6 Hz, 1H), 4.39 (dd, >12 4, 1.4 Hz, H I ). 4.32 - 4.24 (m, 2H), 4.06 (dd, >12.5, 1.5 Hz, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.78 (br t, >9.4 Hz, 1H), 3.71 - 3.64 (m, 1H), 3 54 (d, >0 8 Hz, 1H); LC/MS (ESI) m/e 510 2 [(M+Na) + , calcd for

OH *0-S\a 510.1], /R 1.98 min (Method 1).

Step 2. Preparation of methyl 2-(((2S,3R,4S,5R,6R)-4-azido-5-hydroxy-6- (hydroxymethyl)-2~(phenylthio)tetrahydro-2//-pyran-3~yl)oxy) acetate

Methyl 2~(((4aR,6S,7R,8S,8aR)-8-azido~2-(4-methoxyphenyl)-6-

(phenylthio)hexahydropyrano[3,2-d][l,3]dioxin-7~yl)oxy)ac etate (1.52 g, 3.12 mmol) was dissolved in 80% AcOH (30 mL) and was heated at 40 °C for 45 min. The mixture was concentrated and then reconcentrated from heptane (2 x). The product was purified by column chromatography on silica gel (30% -- 100% ethyl acetate in hexanes; 120 g column) to afford methyl 2-(((2S,3R,4S,5R,6R)-4-azido-5-hydroxy-6-(hydroxymethyl)- 2~(phenylthio)tetrahydro-2//-pyran-3~yl)oxy)acetaie (1.06 g, 2.87 mmol, 92% yield) as a colorless viscous oil. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.57 (dd, >7.3, 1.8 Hz, 2H), 7.41 - 7.30 (m, 3H), 4.72 (d, 7=9.3 Hz, 1H), 4.59 (d, 7=15.6 Hz, 1H), 4.40 (d,

.7=15.6 Hz, ! i I), 4.11 (br s. 1 1 1 ), 4.00 - 3.93 (m, 1H), 3.92 - 3.86 (m, 1 H), 3.80 (s, 3H), 3.77 - 3.64 (m, 2H), 3.56 (t, .7=4.9 Hz, 1H), 2.83 (br s, 1H), 2.21 (br s, 1H); LC./MS (ESI) m/e 392.0 [(M+Na) + , calcd for CisHwNiQeSNa 392.1], (R = 1.56 min (Method 1).

Step 3. Preparation of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-4- azido-2-(phenylthio)tetrahydro-277-pyran-3-yl)oxy)acetate

To a solution of methyl 2-(((2S,3R,4S,5R,6R)-4-azido-5-hydroxy-6- (hydroxymethyl)-2-(phenylthio)tetrahydro-2/7-pyran-3-yl)oxy) acetate (1.02 g, 2,76 mmol) in pyridine (20 mL) was added acetic anhydride (1.563 mL, 16.57 mmol) and

DMAP (0.034 g, 0.276 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was transferred to a separator}- funnel containing ethyl acetete (150 mL). The organic layer was washed with cold 4 N HC1 (2 x 50 mL), saturated aqueous sodium bicarbonate (25 L), and brine (25 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (20% 50% ethyl acetate in hexanes; 80 g column) to afford methyl 2-(((2S,3R,4S,5R,6R)-5- acetoxy-6-(acetoxymethyl)-4-azido-2-(phenylthio)tetrahydro-2 //-pyran-3-yl)oxy)acetate (1 09 g, 2,404 mmol, 87% yield) as a colorless oil. ¾ NMR (400 MHz,

CHLOROF ORM-d) d 7.61 - 7.55 (m, 2H), 7.37 - 7.32 (m, 3H), 5.41 (d, 7=2.8 Hz, 1H), 4.71 (d, .7=9.5 Hz, H i ). 4 59 (d, 7=15.6 Hz, 1H), 4 37 (d, 7=15.6 Hz, 1H), 4.13 (d, 7=6 3

Hz, 21 1). 3.89 - 3.83 (m, 1H), 3.81 (s, 3H), 3.77 (dd, .7=9 5, 3.3 Hz, 1H), 3.58 - 3.51 (m, 1 1 1 ), 2.16 (s, 31 1 ), 2.07 (s, 31 1 ). LC/MS (ESI) m/e 476 1 [(M+Na) + , calcd for

CiHfcN- OsSNa 476.1], te = 1.86 min (Method 1). Step 4 Preparation of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (phenylthio)-4-(4-(3 ,4, 5-trifluorophenyl)- 177- 1 ,2,3 -triazol- 1 -yl)tetrahydro-27/-pyran-3 - yl)oxy)acetate

Methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-4-azido-2- (phenylthio)tetrahydro-27/-pyran-3-yl)oxy)acetate (1.03 g, 2.271 mmol) and 5-ethynyl- 1,2,3-trifluorobenzene (0.709 g, 4.54 mmol) w-ere dissolved in a previously degassed solution of DMF (42 mL) and w-ater (14 mL) and the mixture was placed under argon. Sodium ascorbate (0.450 g, 2.271 mmol) and copper(II) sulfate pentahydrate (0.454 g, 1.817 mmol) (predissolved in 2 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture was filtered through a pad of Celite and the filtrate was transferred to a separator} ' funnel containing saturated aqueous NaHCCfi solution (50 mL). The aqueous layer was extracted with dichlorom ethane (3 x 100 mL). The combined organic layers v ere washed with brine (50 mL), dried over

MgS0 4 , filtered, and concentrated. The filtrate was concentrated and was purified by column chromatography on silica gel (30% 60% ethyl acetate in hexanes; 80 g column) to afford methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (phenylthio)-4-(4-(3 ,4, 5-tri fluorophenyl)- 1 H- 1 , 2,3 -triazol - 1 -y 1 )tetrahydro-2/ -pyran-3 - yl)oxy)acetate (1.30 g, 2.133 mmol, 94% yield) as a white solid. ^ ISiMR (400 MHz, CHLOROFORM-d) d 7 87 (s, 1H), 7 67 - 7.62 (m, 21 1). 7.44 (dd, ./ 8 3, 6.5 Hz, 2H), 7.41 - 7.36 (m, 3H), 5 53 (d, J=2.3 Hz, 1H), 4.84 (d, .7=9.5 Hz, H i). 4.80 (dd, ,7=10.3, 3.0 Hz, i l l). 4 61 - 4.50 (m, 2H), 4.28 - 4.15 (m, 21 1).. 4.09 - 4.03 (m, 2H), 3.59 (s, 31 1 ), 2.07 (s, 3 i f ), 2.05 (s, 31 {); LC/MS (ESI) m/e 632.1 [(M+Na) ÷ , calcd for C % i.v.HVOxSXa 632.1], ta = 2.05 min (Method 1).

Step 5. Preparation of methyl 2-(((2R,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- bromo-4-(4-(3 ,4, 5-trifluoropheny 1 )- 1 7- 1,2,3 -tri azol- 1 -yl)tetrahydro-2/7-pyran-3 - yl)oxy)acetate

To a solution of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2-

(phenylthio)-4-(4-(3 ,4, 5-trifluorophenyJ)- \H- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-277-pyran-3 - yl)oxy)acetate (550 mg, 0.902 mmol) in CH2CI2 (9 mL) (predried over 4 A molecular sieves) at 0 °C was added bromine (10% stock solution in CH2.CI2.) (0.930 mL, 1.805 mmol) dropwise. The reaction mixture was stirred at 0 °C for 1 h. TLC showed that the starting material was consumed. Excess bromine was quenched by the addition of saturated aqueous NaHCCb solution and saturated aqueous Na2.S203 solution (2 mL:6 mL) (the red color disappeared). The mixture was transferred to a separatory funnel containing water (25 mL). The aqueous layer was extracted with dieh!oromethane (3 x 25 mL). The combined organic layers were washed with brine (15 mL), dried over Na2.S04, filtered, and concentrated. The product was purified by column

chromatography on silica gel (20% -----> 50% ethyl acetate in hexanes, 40 g column) to afford methyl 2-(((2R,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2-bromo-4-( 4-(3,4,5- trifluorophenyl)- li/-l ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-3 -yl)oxy)acetate (447 mg, 0.770 mmol, 85% yield) as a white foam. The product was stored in the freezer until it was used. ! H NMR (400 MHz, CHLOROFORM-d) d 8.01 (s, 1H), 7.47 (dd, ,7=8.3, 6.5 Hz, 2H), 7 18 (d, 7=3.5 Hz, i l l ), 5.63 - 5 57 (rn, H I ), 5.15 (dd, 7=10.7, 2.9 Hz, Hi), 4.74 (dd, 7=10.8, 3.8 Hz, 1H), 4.65 (t, 7=6.7 Hz, 1H), 4.27 - 4.10 (m, 4H), 3.76 (s, 3H), 2.08 (s, 31 1), 2.07 (s, 3H); LC/MS (ESI) m!e 580.1 [(M+H) + , caled for C M 1 VBHOVOK 580.1 ], tR = 2.02 min (Method 1).

Step 6

To a solution of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//-pyran-4-ol

(1 15 mg, 0.656 mmol) in ethyl acetate (3 mL) and sodium carbonate solution (1 M, aq)

(3 mL, 3.00 mmol) was added methyl 2-(((2R,3R,4S,5R,6R)-5-acetoxy-6- (acetoxymethyl)-2-bromo-4-(4-(3, 4, 5-tri fluorophenyl)-!//- 1,2, 3-triazol-l-yl)tetrahydro- 2Z/-pyran-3-yl)oxy)acetate (438 mg, 0.755 mmol) in ethyl acetate (3 mL) via cannula. Tetrabutylammonium hydrogen sulfate (891 mg, 2.63 mmol) was added and the reaction mixture was stirred vigorously at room temperature for 2.5 h. The mixture was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (25 mL). The aqueous layer was extracted with ethyl acetate (3 x 25 ml,). The combined organic layers were washed with brine (25 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (40% 70% ethyl acetate in hexanes; 24 g column) to afford methyl 2-(((2S,3R,4S,5R,6R)-5- acetoxy-6-(acetoxymethyl)-2-(((3R,4R,5S)-5-azido-4-hydroxyte trahydro-2//-pyran-3- yl)thio)-4-(4-(3,4,5-trifluorophenyl)-17 -l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3- yl)oxy)acetate (445 mg, 0.660 mmol, 100% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 7 86 (s, 1H), 7 45 (dd, 7=7.5, 6.5 Hz, 2H), 5.54 (d, 7=3 0 Hz, 1H), 4.83 - 4.72 (m, 2H), 4.57 - 4.47 (m, 1H), 4.40 (d, 7=15.8 Hz, 1H), 4.26 - 4.05 (m, 5H), 4.01 (dd, 7=11.5, 5.0 Hz, 1H), 3.69 - 3 63 (m. 1 1 1 ), 3.61 (s, 3H), 3.60 - 3.50 (m, H i), 3.24 (t, 7=11.9 Hz, 1H), 3.12 (t, 7=11.2 Hz, H i). 2.99 (ddd, 7=1 1.7, 9.7, 4.9 Hz, 1 1 1 ). 2.14 (s, 31 1 ).. 2.08 (s, 3H); LC/MS (ESI) m/e 675 2 [(M I f ) , ca!cd for CieHsoFsNeOioS 675.2], t R = 2.04 min (Method 1). Preparation of final products

Example 1. Preparation of (2R,3R,4S,5R,6S)-4-(4-(3-fluorophenyl)-lii-l,2,3-triazol-l- yl)~6~(((3 R,4R, 5 S)-5-(4-(3 -fluorophenyl)- IH- 1 ,2,3 -triazol - 1 -y l)-4-hydroxy tetrahydro- 2i/-pyran-3-yl)thio)-2-(hydroxymethyl)-5-methoxytetrahydro-2 //-pyran-3-ol

Example 1

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)-li7-l,2,3- triazol-l-yl)-6-(((3R,4R,5S)-5-(4-(3-fluorophenyl)-li -l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2/7-pyran-3-yl)†hio)-5-methoxy†etrahyd ro-2/ -pyran-2-yl)methyl acetate

((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hydroxy tetrahydro-2i7- pyran-3-yl)thio)-4-(4-(3-fluorophenyl)-li7-l,2,3-triazol-l-y l)-5-methoxytetrahydro-2/7- pyran-2-yl)methyl acetate (40 mg, 0 069 mmol) and l-ethynyl-3-fluorobenzene (13.24 mg, 0 1 10 mmol) were dissolved in a previously degassed solution of DMF (1.5 mL) and water (0 500 mL) and the mixture was placed under argon. Sodium ascorbate (16 38 mg, 0.083 mmol) and copper(II) sulfate pentahydrate (24 08 mg, 0.096 mmol) (predissolved in 0.5 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 15 mL). The combined organic layers were washed with brine (15 mL). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% methanol/80% hexanes 80% ethyl acetate containing 5% methanol/20% hexanes; 24 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3~iluorophenyl)-l//-l,2,3- triazol-l -yl)-6-(((3R,4R,5S)-5-(4-(3-fluorophenyl)-li -l,2,3-triazol-l -yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-5-methoxytetrahydro-2 /i-pyran-2-yl)methyl acetate (45 mg, 0.064 mmol, 93% yield) as a white solid. r H NMR (400 MHz,

CHLOROFORMS) d 7.94 (s, 1H), 7.88 (s, 1H), 7.62 - 7.53 (m, 4H), 7.41 (tdd, , 7=7.9, 6.0, 2.0 Hz, 21 1 ), 7.1 1 - 7.02 (m, 2H), 5 53 (d, ./ 3.0 Hz, 1 1 1), 4.76 - 4.70 (m, 21 1 ). 4.56 - 4.48 (m, 1H), 4.38 (dd, J=1 1.5, 4.8 Hz, 1H), 4.34 - 4.28 (m, 2H), 4.27 - 4.22 (m, 1H), 4.20 - 4.10 (m, 4H), 4 09 - 4 03 (m, H I ), 3.53 (t, ./ 1 1.9 Hz, i l l ). 3 35 (s, 3H), 3 17 (ddd. ,/ 1 1 .8, 9.8, 5.0 Hz, ! H), 2.11 (s, 3H), 1.93 (s, 31 ! ); 1.C7MS (ESI) rule 701 .2 [(M+H) + , calcd for C H LT’M.GKS 701.2], /R = 1.97 min (Method 2).

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)-l/ - l,2,3-triazol-l-yl)-6-(((3R,4R,5S)-5-(4-(3-fluorophenyl)-li/ -l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-5-methoxytetrahydro-2 //-pyran-2-yl)methyl acetate (45 mg, 0.064 mmol) in THF (0.75 mL) and MeOH (0.75 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.073 mL, 0.321 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.2 mL). The mixture was concentrated and was taken up in dioxane/methano!/water and was filtered and purified by reverse phase HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6SV4-(4-(3~fluQrophenyi )-!//- l,2,3-triazol-l-yl)-6-(((3R,4R,5S)-5-(4-(3-fluorophenyl)-li7 -l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-2-(hydroxymethyl)-5-m ethoxytetrahydro-2//- pyran-3-ol (30 mg, 0.049 mmol, 76% yield) as a white amorphous solid. f H NMR (500 MHz, DMSO-de) d 8.90 (s, 1H), 8.76 (s, 1H), 7.77 (d, J= 7.8 Hz, 1H), 7.74 - 7.68 (m,

2H), 7.67 - 7.61 (m, H i ), 7.52 (tdd, ,7-8.0, 6.1 , 3.5 Hz, 2H), 7.18 (tdd, 7 8.2, 5.5, 2 6 Hz, 2H), 4.95 (dd, 7=10.5, 3.0 Hz, 1H), 4.83 (d, 7=9.3 Hz, 1H), 4.64 - 4.55 (m, 1H), 4.25 (dd, 7=11.6, 4.7 Hz, H i), 4.13 (dd, 7=11.1, 4.9 Hz, H i), 4.04 (t, 7=9.9 Hz, H I ). 3 98 - 3.91 (m, 2H), 3.85 - 3.77 (m, 2H), 3.58 - 3.50 (m, 3H), 3.30 (dt, 7=10.8, 5.5 Hz, 2H), 3.25 (s,

3H); LC/MS (ESI) mle 617.2 [(M+H) + , calcd for C^ftiFiNeOeS 617 2], fe = 1 79 min (Method 2); HPLC (Method 3): t& = 10.01 min; HPLC (Method 4): t& = 8.68 min. hGaf- 3 1C so = 0.060 mM.

Example 2 Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-fluorophenyl)-l/ - l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3-yi)thio) -2-(hydroxymethyl)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-17/-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-ol

Example 2 Step 1. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-azidotetrahydro- 2/:/-pyran~3~yl)thio)~2-(acetoxymethyl)-5-methoxy-4-(4-(3,4, 5-triiluorophenyl)-!i/~

1 ,2,3 -triazol- 1 -yl)tetrahydro-27/-pyran-3 -yl acetate

To a solution of (-)-(3S,4R,5R)-3-azido-5-mercaptotetrahydro-2//-pyran-4-yl acetate (43 mg, 0.198 mmol) in ethyl acetate (3 mL) was added sodium carbonate (0.396 mL, 0.792 mmol), ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-5-methoxy-4-(4-(3, 4,5- trifluorophenyl)-l//-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran -2-yl)methyl acetate (114 mg, 0.218 mmol), and tetrabutyl ammonium hydrogen sulfate (269 mg, 0.792 mmol). The reaction mixture was stirred vigorously at room temperature for 1.5 h. The mixture was transferred to a separatory funnel containing saturated aqueous NaTICCb solution (5 ml.). The aqueous layer was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (5 mL), dried over MgS0 4 , filtered, and concentrated .

The product was purified by column chromatography on silica gel (0% 70% ethyl acetate in hexanes; 24 g column) to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy- 5-azidotetrahydro-2/ -pyran-3-yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5- trifluorophenyl)-l//-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran -3-yl acetate (101 mg, 0.153 mmol, 77% yield). l B NMR (400 MHz, CHLOROFORM-d) d 7.81 (s, 1H), 7 46 (dd, .7=8.3, 6.5 Hz, 2H), 5.51 (dd, .7=3.1, 0.9 Hz, 1H), 5.06 (dd, .7=10.8, 9.3 Hz, 1H), 4.69 - 4.61 (m, 2H), 4.27 - 4.01 (m, 6H), 3.69 (ddd, 7=10.7, 9.2, 5.3 Hz, H i), 3.59 - 3 49 (m, 1H), 3.31 (s, 3H), 3.25 (t, 7=11.2 Hz, 1H), 3.14 (td, 7=11.0, 4.8 Hz, 1H), 2.20 (s, 3H), 2.10 (s, 31 1), 2.08 (s, 3H); LC/MS (ESI) m/e 658.6 [(M+H) + , calcd for (>,! j M7N7LS

659.2], hi = 2.10 min (Method 2).

Step 2. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy~5-(4-(3- fluorophenyi)- 1/7- 1 ,2,3 -triazol- 1 -yl)tetrahydro-277-pyran-3 -yl)thio)-2-(acetoxymethyl)- 5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l /7-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yl acetate

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-Acetoxy-5-azidotetrahydro- 27/-pyran-3- yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5-trifluoroph enyl)-l/7-l,2,3-triazol-l - yl)tetrahydro-27/-pyran-3-yl acetate (101 mg, 0.153 mmol) and l-ethynyl-3- fluorobenzene (55.3 mg, 0.460 mmol) were dissolved in a previously degassed solution of DMF (3 mL) and water (1.000 mL) and the mixture was placed under argon. Sodium ascorbate (30.4 mg, 0.153 mmol) and copper(II) sulfate pentahydrate (26.8 rng, 0.107 mmol) (predissolved in 0.4 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The reaction mixture was filtered through a pad of ( ' elite and the filtrate was transferred to a separatory funnel containing saturated aqueous NaHCCh solution (10 mL). The aqueous layer was extracted with dichloromethane (3 x 15 mL). The combined organic layers were washed with brine (TO mL), dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (20% 75% ethyl acetate in hexanes; 24 g column) to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-(4-(3-fluorop henyi)-li/-l,2,3-triazol-l- yl)tetrahydro-2i7-pyran-3-yl)thio)-2-(aceiOxymethyl)~5~metho xy-4-(4-(3,4,5- trifluorophenyl)-177-l ,2,3-triazol-l-yl)tetrahydro-277-pyran-3-yl acetate (85 mg, 0.109 mmol, 71% yield) as a white solid. 1 H NMR (400 MHz, CHLOROFORM-d) d 7.91 (s, 1H), 7.83 (s, 1H), 7.65 - 7.60 (m, 1H), 7.60 - 7.56 (m, 1H), 7.46 (dd, 7=8.0, 6.3 Hz, 2H), 7.44 - 7.38 (m, 1H), 7.11 - 7.04 (m, IH), 5.61 - 5.49 (m, 2H), 4.85 (td, 7=10.2, 4.9 Hz, 1 1 1 ), 4.70 (d, 7=9.5 Hz, I H), 4.65 (dd, 7=10.2, 3.1 Hz, 1 H), 4.38 (t, 7=4.0 Hz, H i ), 4.36 - 4.33 (m, IH), 4.29 - 4.21 (m, 2H), 4.17 - 4.08 (m, 2H), 3.98 (t, 7=11.2 Hz, IH), 3.77 (t,

7=1 1 .8 Hz, I H), 3.37 - 3.31 (m, IH), 3 30 (s, 3H), 2 1 1 (s, 3H), 2 1 1 (s, 3H), 2 01 (s, 3H); LC/MS (ESI) mie 778.4 [(M+H) + , calcd for C M %!· :\70 ;S 779.2], ίk = 2.12 min (Method 2). Step 3

To a solution of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-(4-(3- fluorophenyl)- Hi- 1 ,2, 3 -triazol- 1 -yi)tetrahydro-2//~pyran-3 -yl)thio)-2-(acetoxymethyl)- 5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l -l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3-yl acetate (80 mg, 0.103 mmol) in methanol (1.0 mL) and THF (1.0 mL) at 0 °C was added sodium methoxide (25% wt in MeOH) (0.1 17 mL, 0.514 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.2 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3- fluorophenyl)- IH-l ,2, 3 -triazol- 1 -yl)-4-hydroxytetrahydro-2i/-pyran-3 -yl)thio)-2- (hydroxymethyl)-5-methoxy-4-(4-(3 , 4, 5-tri fluorophenyl)- 177-1 ,2, 3-triazol-l- yl)tetrahydro-2i/-pyran-3-ol (26 mg, 0.039 mmol, 38% yield) as a white amorphous solid. 4 1 NMR (500 MHz, METHANOL-dr) 6 8.69 (s, 1 1 1 ), 8.50 (s, 1 1 1), 7.73 - 7.64 (m, 3H), 7.63 - 7.57 (m, 1H), 7.47 (td, 7=8.0, 6.0 Hz, 1H), 7.13 - 7.06 (m, 1H), 4.94 (dd,

7=10.5, 3.0 Hz, 1 1 1), 4.80 (d, 7 9 3 Hz, 1 1 1 ), 4.71 - 4.62 (m, 1 1 1 ).. 4.32 (dd, 7=12.0, 5.0 Hz, 1H), 4.23 (dd, 7=11.1, 5.0 Hz, 1H), 4.13 - 4.04 (m, 31 1). 3.95 (t, 7=11.1 Hz, 1H), 3.87 - 3.79 (m, 2H), 3.76 - 3.70 (m, 1H), 3.62 (t, ,7=1 1.7 Hz, 1H), 3.35 (br d, 7 = 5.3 Hz, 1 H), 3.32 (s, 3H); LC/MS (ESI) m/e 652.5 [(M+H) + , calcd for CrHMLNeOeS 653 2], fo = 1.99 min (Method 2); HPLC (Method 3): /R = 10.54 min; HPLC (Method 4): /K 9.33 min h Gal-3 ICso = 0.046 mM.

Example 3. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(pyridin-2 yl)-l//-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-2-(hydroxy methyl)-5-methoxy 4-(4-(3 ,4, 5-tri fluorophenyl)- 1 H- 1 ,2,3 -triazol - 1 -yl)tetrahydro-2/7-pyran-3 -ol

Example 3

Step 1 Preparation of (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-( 4- (pyridin-2-yl)-li/-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3 -yl)thio)-5-methoxy-4-(4- (3,4,5 -trifluoropheny 1 )- f H- 1 ,2, 3 -tri azol- 1 -yl)tetrahy dro-2/7 -py ran-2-yl)methy 1 acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)- l//-l,2,3-triazol-l- yl)tetrahydro-2Z/-pyran-2-yl)methyl acetate (75 mg, 0.122 mmol) and 2-ethynylpyridine (37.6 mg, 0.365 mmol) were dissolved in a previously degassed solution of DMF (2.1 mL) and water (0.7 raL) and the mixture was placed under argon. Sodium ascorbate (28.9 mg, 0.146 mmol) and copper(II) sulfate pentahydrate (42.5 mg, 0.170 mmol) (predissolved in 0 3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 niL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Several milliliters of brine was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The separately funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol; 24 g column) to afford (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-

4-hydroxy-5-(4-(pyridin-2-yl)-l//-l,2,3-triazol-l-yl)tetr ahydro-2/ -pyran-3-yl)thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-177-l,2,3-triazoJ-l-yl) tetrahydro-2/7-pyran-2- yl)methyl acetate (80.6 mg, 0.112 mmol, 92% yield) as a white solid. [a]o +31.9, (c = 0.245, DM!· ): ' l l \MR (500 MHz, CHLOROFORM-d) 6 8.63 - 8.56 (m, 1 1 1 ). 8 31 (s, 1H), 8.19 (d, .7=7.9 Hz, 1 1 1). 7.84 - 7.78 (m, 2H), 7.47 (dd, 7=8.1, 6.4 Hz, 2H), 7.28 - 7.24 (m, 1H), 5.51 (d, 7=3.1 Hz, 1H), 4.70 (d, 7=9.6 Hz, 1H), 4.65 (dd, 7=10.2, 3.1 Hz, 1 1 1 ), 4.61 - 4.52 (m, 1 1 1 ), 4.38 (dd, 7=11 6, 4 9 Hz, 1 1 1 ), 4.31 (dd, 7=11 9, 5 0 Hz, 1 1 1 ), 4.25 (t, 7=10.0 Hz, 1H), 4.21 - 4.00 (m, 5H), 3.51 (t, 7=12.0 Hz, 1H), 3.36 (s, 3H), 3.18

(ddd, 7=1 1.9, 9.8, 5.0 Hz, 1 1 1 ), 2.10 (s, 3H), 1.93 (s, 31 1 ); LC/MS (ESI) m/e 720.2

[(M+H) + , calcd for C31H33F3N7Q8S 720.2], fii = 1.90 min (Method 1).

Step 2

To a suspension of (+)-(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-(4-

(pyridin-2-yl)-17/-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-3-yl)thio)-2-(acetoxymethyl)-

5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1/7- 1 ,2,3 -triazol- 1 -yDtetrahy dro-2//-pyran-3 -yl acetate (34.5 mg, 0 045 mmol ) in THF (0.7 mL) and MeOH (0.7 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.052 mL, 0.226 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was

concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (+)-(2R,3R,4S,5R,6S)- 6-(((3R,4R,5S)-4~hydroxy-5-(4-(pyridin-2~yl)-l/>l,2,3-tri azol-i-yl)tetrahydro-2/> pyran~3-yi)thi o)-2-(hydroxymethyl)-5-methoxy-4-(4-(3,4,5-tri fluorophenyl)- IH-l, 2, 3- triazol- l-yl)tetrahydro-2/>pyran-3-ol · TEA (31.3 mg, 0.041 mmol, 91% yield) as a white amorphous solid. [a]o 22 +45.9, (c = 0.330, DMF); ¾ NIVIR (500 MHz, DMSO-de) d 8.98 (s, ill), 8.76 (s, i l l ), 8.62 (br d, ./ 4.7 Hz, IH), 8.06 (d, .>=7 8 Hz, 1H), 7.94 (br t, >=7.7 Hz, IH), 7.89 - 7.82 (m, 2H), 7.42 - 7.35 (m, IH), 5.00 - 4.96 (m, IH), 4.85 (br d, >=9.3 Hz, IH), 4.65 (td, >=10.3, 4.9 Hz, 2H), 4.23 (br dd, J= 11.7, 5 0 Hz, 2H), 4.1 1 (br dd, >=10 5, 5 0 Hz, 21 ! ), 4.02 - 3.93 (m, 4H), 3.88 - 3.82 (m, IH), 3.80 (br t, >=6.2 Hz, IH), 3.54 (br dd, >=10.1, 4.0 Hz, 3H), 3.25 (s, 3H); LC/MS (ESI) m/e 636.2 |(M 1 1 ) , calcd for C27H29F3N7O6S 636.2], fe == 1 .74 min (Method 1 ); HPLC (Method 1 ): /, = 5.94 min; HPLC (Method 2): tv, = 6.06 min. hGai-3 ICso = 0.019 uM. Example 4. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(pyrimidin- 2-yl)- 1 H- 1 ,2,3 -triazol - 1 -yl)tetrahydro-2/7-pyran-3 -yl)thio)-2-(hydroxymethyl)-5- m ethoxy-4-(4-(3 ,4, 5 -trifluoropheny 1 )- \H~ 1 ,2, 3 -tri azol- 1 -yl)tetrahy dro-2/7 -py ran-3 -ol

Example 4 Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4- (pyrimidin-2-yl)-l//-l,2,3-triazol-l -yl)tetrahydro-2ff-pyran-3-yl)thio)-5-methoxy-4-(4- (3 ,4, 5-trifluoropheny!)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7-pyran-2-yl)methyl acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1,2,3 -triazol- 1 - yf)tetrahydro~2/:/~pyran~2-yf)methyl acetate (28 mg, 0 045 mmol) and 2- ethynylpyrimidine (9.46 mg, 0.091 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.3 mL) and the mixture was placed under argon. Sodium ascorbate (9 00 mg, 0.045 mmol) and copper(II) sulfate pentahydrate (9.07 mg, 0.036 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. The mixture was transferred to a separatory funnel. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added along with several milliliters of brine resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 ml.). The combined organic layers were washed with brine (10 mL). The separately funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgSCfi, filtered, and concentrated. The product was purified by column chromatography on silica gel (50% ethyl acetate containing 5% methanol/50% hexanes 100% ethyl acetate containing 5% methanol; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4- hydroxy-5 -(4-(pyrimidin-2-y 1 )- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7-pyran-3 -yl)thi o)-5- methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 177-1, 2, 3-triazol-l-yl)tetrahydro-277-pyran-2- yl)m ethyl acetate (27.5 mg, 0.038 mmol, 84% yield) as a white solid. r H NMR (400

MHz, CHLOROFORM-d) 6 8.84 (d, 7 5.0 Hz, 2H), 8.42 (s, 1H), 7.83 (s, 1H), 7.47 (dd, J= 8 3, 6 5 Hz, 21 !), 7.28 - 7.23 (m, S i !), 5.51 (d, .7=3.0 Hz, 1 ! !). 4 74 - 4.64 (m, 21 1). 4.57 (td, 7=10.3, 4.8 Hz, 1H), 4.41 (dd, J=11.3, 5.0 Hz, 1H), 4.35 - 4.04 (m, 8H), 3.57 - 3.47 (m, 2H), 3.36 (s, 3H), 3.17 (ddd, 7=1 1.9, 9.7, 5.0 Hz, H i ). 2.1 1 (s, 3H), f .95 (s, 3H); LC/MS (ESI) m/e 721.2 [(M+H) + , calcd for C30H32F3N8O8S 721.2], fe = 1.79 min (Method 1).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-

(4-(pyrimidin-2-yl)-l/ - 1,2, 3-tri azol- l-yl)tetrahydro-2//-pyran-3-yl)thio)-5-methoxy-4- (4-(3,4,5-trifluorophenyl)-l77-l,2,3-triazol-l-yl)tetrahydro -277-pyran-2-yl)methyl acetate (24 mg, 0.033 mmol) in THE (0.7 niL) and MeOH (0.7 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.038 mL, 0.167 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HQ (0.1 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,0S)-6-(((3R,4R,5S)-4- hydroxy-5-(4-(pyrimidin-2-yl)-17 ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yl)thio)-2- (hydroxymethyl)-5-me†hoxy-4-(4-(3,4,5-trifluorophenyl)-l// -l,2,3-triazol-I - yl)tetrahydro-277-pyran-3-ol (18.6 mg, 0.029 mmol, 86% yield) as a white amorphous solid. 4 1 NMR (500 MHz, DMSO-de) d 8.96 (s, 1H), 8.80 (s, 1 H), 8.05 (s, 1 H), 8.00 (d, J=7.8 Hz, 1H), 7.89 - 7.80 (m, 2H), 7.66 - 7.59 (m, 1H), 7.55 (d, =7.8 Hz, 1H), 7.1 1 (t, ,/ 55.8 Hz, 1 1 1), 4.98 (d l, ,/ 10 5, 2,9 Hz, 1 1 1), 4.83 (d, .7=9.3 Hz, 1 1 1 ). 4 65 - 4.57 (m,

1 1 1 ), 4.26 (br dd, J=l 1.7, 4.8 Hz, 1H), 4.14 (br dd, .7=11.0, 4.9 Hz, 1 1 1 ). 4.02 - 3.93 (m, 3H), 3.85 - 3.78 (m, 2H), 3.58 - 3.50 (m, 3H), 3.29 (dd, .7=10.7, 4 7 Hz, H i), 3.25 (s,

3H); LC/MS (ESI) m/e 637.2 [(M+H) + , calcd for C2.6H28 FsNeOeS 637.2], /s = 1.84 min (Method 1), HPLC (Method 1): /R ::: 6.14 min, HPLC (Method 2): /R = 5.75 min. hGal-3 ICso = 0.018 mM.

Example 5. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3- (di fl uoromethy l)ph enyl)- 1 H- 1,2,3 -tri azol - 1 -yl)-4-hy droxytetrahy dro-2/7-py ran-3 - yl)thio)-2-(hydroxymethyl)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 177-1, 2, 3-triazol-l- yl)tetrahydro-2/7-pyran-3-ol

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3- (difluoromethyl)phenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-2/7-pyran-3 - yl)thi o)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- IH- 1 , 2,3 -triazol- 1 -y 1 )tetrahydro-2//~ pyran-2-yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)-!//- 1,2, 3-triazol-l- yl)tetrahydro-2i/-pyran-2-yl)methyl acetate (48 mg, 0.078 mmol) and 1- (difluoromethyl)-3-ethynylbenzene (23.69 mg, 0.156 mmol) were dissolved in a previously degassed solution of DMF (0.9 rnL) and water (0.3 mL) and the mixture was placed under argon. Sodium ascorbate (15.42 mg, 0.078 mmol) and copper(II) sulfate pentahydrate (15.55 mg, 0.062 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. The mixture was transferred to a separatory funnel. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added along with several milliliters of brine resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (30% ethyl acetate containing 5% methanol/70% hexanes 70% ethyl acetate containing 5% methanol/30% hexanes; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6- (((3R,4R,5S)-5-(4-(3-(difluoromethyl)phenyl)-l//-l,2,3-triaz ol-l -yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5 -trifluorophenyl)-l/7- l,2,3~triazo!~l-y!)tetrahydro~2/: -pyran~2-y!)methyl acetate (56.8 mg, 0.074 mmol, 95% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 8.01 - 7.95 (m, 31 1). 7.82 (s, 1H), 7.61 - 7.44 (m, 4H), 6.72 (t, ,7=56.5 Hz, 1H), 5.52 (d, ,7=2.8 Hz, 1H), 4.73 - 4.62 (m, 2H), 4.53 (td, 7=10.3, 4.8 Hz, 1H), 4.40 (dd, 7=11.5, 4.8 Hz, H i ), 4.35 - 4 23 (m, 3H), 4.21 - 4.04 (m, 4H), 3.57 - 3.49 (m, 2H), 3.36 (s, 3H), 3.16 (ddd, 7=11.9, 9.7,

5.0 Hz, H i), 2.1 1 (s, 3H), 1 93 (s, 3H); LC/MS (ESI) m!e 769.2 | (M i 1 ) . calcd for CssKwFsNeOsS 769.2], fe = 1.96 min (Method 1).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3-

(di fl uoromethy l)ph enyl)- 17/- 1,2,3 -tri azol - 1 -yl)-4-hy droxytetrahy dro-2/7-py ran-3 - yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-17/-l,2,3-tr iazol-l-yl)tetrahydro-27/- pyran-2-yl)methyl acetate (47 mg, 0 061 mmol) in THE (0.7 ml,) and MeOH (0.7 ml/) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.070 mL, 0.306 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h.

The mixture was partially neutralized by the addition of 1 N HC1 (0.2 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-(difluoromethyl)p henyl)-17/-l,2,3-triazol-l- yl)-4-hydroxytetrahydro-2/7-pyran-3-yl)thio)-2-(hydroxymethy l)-5-methoxy-4-(4-(3,4,5- trifiuorophenyl)-l77-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-ol (38 mg, 0.055 mmol, 90% yield) as a white amorphous solid. ‘H NMR (500 MHz, DMSO-de) d 8.96 (s, 1H), 8.80 (s, H I ), 8.05 (s, H I ), 8.00 (d, ./ 7.8 Hz, i l l ). 7 89 - 7.80 (m, 21 1), 7.66 - 7.59 (m, 1 1 1 ), 7.55 (d, ,7=7.8 Hz, 1H), 7.1 1 (t, ,7=55.8 Hz, !H), 4.98 (dd, 7=10.5, 2.9 Hz, !H), 4.83

(d, 7=9.3 Hz, 1H), 4 65 - 4.57 (m, 1 1 1 ), 4.26 (br dd, 7=11.7, 4.8 Hz, H I ), 4.14 (br dd, 7=11.0, 4.9 Hz, 1H), 4.02 - 3.93 (m, 3H), 3.85 - 3.78 (m, 2H), 3.58 - 3.50 (m, 31 1). 3.29 (dd, ,7=10.7, 4.7 Hz, 1H), 3.25 (s, 3H); LC/MS (ESI) mie 685.2 [(M+H) + , calcd for C29H30F5N6O6S 685.2], iR = 2.04 min (Method 1), HP1.C (Method 1 ): i\<. = 8.16 min, HPLC (Method 2): 7R = 7.50 min. liGai-3 IC50 = 0.051 mM.

Example 6 Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3- (difluoromethoxy)phenyl)H 77-1,2, 3-triazol-l-yl)-4-hydroxytetrahydro-27/~pyran-3- yl)thio)-2~(hydroxymethyl)-5-methQxy-4-(4~(3, 4, 5-tri fluorophenyl)- 1/7- 1,2, 3~triazol~l- yl)tetrahy dro-277-pyran-3 -ol

Example 6

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3- (difluoromethoxy)phenyl)-1 ,2,3~triazol~l-y!)-4-hydroxytetrahydro~27/~pyran-3- yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-I7 -l,2,3-triazol-l-yl)tetrahydro-2i/- py ran -2-y 1 )m ethyl acetate

(+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//~pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifiuorophenyl)- l/7-!,2,3~triazol-l- yi)tetrahydro-277 pyran-2-yl)methyl acetate (23.1 mg, 0.037 mmol) and 1- (difluoromethoxy)-3-ethynylbenzene (12.60 mg, 0.075 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.3 raL) and the mixture was placed under argon. Sodium ascorbate (7.42 mg, 0.037 mmol) and copper(II) sulfate pentahydrate (7.48 mg, 0.030 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. The mixture was transferred to a separatory funnel. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added along with several milliliters of brine resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (30% ethyl acetate containing 5% methanol/70% hexanes -- 70% ethyl acetate containing 5% methanol/30% hexanes, 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6- (((3R,4R,5S)-5-(4-(3-(difluoromethoxy)phenyl)-li/-l,2,3-tria zol-l-yl)-4- hydroxyteirahydro-2//~pyran-3-yl)thio)-5-methoxy-4~(4-(3,4,5 -trifiuorophenyl)~l/:/- l,2,3-triazol-l-yl)tetrahydro-27/-pyran-2-yl)methyl acetate (26.7 mg, 0.034 mmol, 91% yield) as a white solid 1 H NMR (400 MHz, CHLOROFORM-d) 6 7 94 (s, i f if 7 83 (s, 11 1), 7.67 (d, ,/ 7.8 Hz, i l l), 7.61 (s, i l l), 7.51 - 7 41 (m, 3H), 7.13 (dd, ,7-8.0, 2.3 Hz, 1H), 6.60 (t, 7=73.8 Hz, 1H), 5.51 (d, 7=3.0 Hz, 1H), 4.72 - 4.64 (m, 2H), 4.52 (td,

./ 10.5, 4.9 Hz, 1H), 4.39 (dd, 7=11.5, 4.8 Hz, 1H), 4.34 - 4 22 (m, 31 ! ), 4.21 - 4.01 (m, 5H), 3.53 (t, 7=11.9 Hz, 1H), 3.36 (s, 3H), 3.16 (ddd, 7=11.7, 10.0, 5.1 Hz, 1H), 2.11 (s, 31 1), 1.93 (s, 3H); LC/MS (ESI) m/e 785.1 [(M+H) + , calcd for C33H34F5N6O9S 785.2], /¾

= 2.13 min (Method 1).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3- (difluoromethoxy)phenyl)-l/7-l,2,3-triazol-l-yl)-4-hydroxyte trahydro-2//-pyran-3- yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-17 -l,2,3-triazol-l-yl)tetrahydro-2i - pyran-2-yl)methyl acetate (25 mg, 0 032 mmol) in THF (0.7 mL) and MeOH (0.7 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.036 mL, 0.159 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.1 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-(difluoromethoxy)phe nyl)-l /-l ,2,3-triazol-l- yl)-4-hydroxytetrahydro-2/7-pyran-3-yl)thio)-2-(hydroxymethy l)-5-methoxy-4-(4-(3,4,5- trifluorophenyl)~l/:/-l ,2,3-triazo!-l-yl)tetrahydro-2/:/-pyran-3-oi (19 6 mg, 0.028 mmol, 87% yield) as a white amorphous solid Ή NMR (500 MHz, DMSO-de) d 8.99 (s, 1H),

8.78 (s, H I ), 7.86 (dd, .7=8.9, 6.8 Hz, 2H), 7 72 (d, ./ 7 9 Hz, 1H), 7 63 (s, i l l ), 7 53 (t, .7=7.9 Hz, 1H), 7.33 (t, J=73.9 Hz, IH), 7.16 (dd, .7=8.2, 2.1 Hz, 1H), 4.98 (dd, .7=10.6, 2 8 Hz, IH), 4.83 (d, .7=9.3 Hz, IH), 4.63 - 4.56 (m, IH), 4 25 (dd, J=11.7, 5.0 Hz, IH), 4.13 (hr dd, .7=11 1 , 5 1 Hz, I H), 4.01 - 3.91 (m, 3H), 3 84 - 3.76 (m, 2H), 3.56 - 3.49 (m, 3H), 3.32 - 3.27 (m, IH), 3.25 (s, 3H); LC/MS (ESI) m!e 701.2 [(M+H) + , calcd for

C29H30F5N6O7S 701.2], tR = 2.02 min (Method 1), HPLC (Method 1 ): i\<. = 8.25 min, HPLC (Method 2): /R = 7.56 min. liGal-3 IC50 = 0.058 mM.

Example 7 Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(5-fluoropyrimidin-2- y 1 )- IH- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-2T7-pyran-3 -yl)thio)-2-(hydroxymethyl)-

5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triazol- l -yl)tetrahydro-2//-pyran-3-ol

Example 7

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(5

fluoropyrimidin-2-yl)-l /-l,2,3-triazol-l -yl)-4-hydroxytetrahydro-2//-pyran-3-yl)thio)-5- m ethoxy -4-(4~(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-277-pyran-2- yl)m ethyl acetate

(+)-f(2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2/7~pyran~3 ~yl)thio)~5~methoxy~4-(4-(3 ,4, 5-irifluorophenyl V 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2i7-pyran-2-yl)methyl acetate (17 mg, 0.028 mmol) and 2-ethynyl-5- fluoropyrimidine (10.10 mg, 0.083 mmol) were dissolved in a previously degassed solution of DMF (0.6 niL) and water (0.2 mL) and the mixture was placed under argon. Sodium ascorbate (5.46 mg, 0.028 mmol) and copper(II) sulfate pentahydrate (5.51 mg, 0.022 mmol) (predissolved in 0.3 rnL water) were added and the reaction mixture was stirred at room temperature for 14 h. The solid clinging to the stir bar was dissolved by the addition of dichloromethane and methanol. The mixture was transferred to a separately funnel containing water (5 l .) and saturated aqueous sodium bicarbonate (5 mL) resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the orange residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgSOy filtered, and concentrated. The product w'as purified by column chromatography on silica gel (50% ethyl acetate containing 5% methanol/50% hexanes— > 100% ethyl acetate containing 5% methanol; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6- (((3R,4R,5S)-5-(4-(5-fluoropyrimidin-2-yl)-177-l,2,3-triazol -l-yl)-4-hydroxytetrahydro- 2/7~pyran~3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-277-pyran-2-yl)methyl acetate (15.9 mg, 0.022 mmol, 78% yield) as a white solid. ¾ NMR (500 MHz, DMSO-de) 6 8.98 (s, 2H), 8.96 (s, 1 1 1), 8.86 (s, 1 1 1), 7.81 (dd, J= 8 6, 6 8 Hz, 2H), 5.72 id, .7=7.2 Hz, l i !), 5.39 id, .7=2.7 Hz, 1 1 1 ), 5.27 (dd, .7=10.5, 3.1 Hz, 1H), 5.04 (d, .7=9.3 Hz, 1H), 4.68 (td, .7=10.4, 5.0 Hz, 1H), 4.35 (t, .7=6.3 Hz,

1 1 1 ), 4.21 (dd, .7=11 7, 4.7 Hz, H I ), 4.15 (dd, .7=10 9, 5 0 Hz, 1 1 1 ). 4.1 1 - 4.01 (m, 41 1 ), 3.88 (t, .7=11.1 Hz, H i ). 3.58 (t, 7=11.6 Hz, 1H), 3.26 (s, 31 1 ). 3.25 - 3.20 (m, H i ). 2.04 (s, 3H), 2.03 (s, 3H); LC/MS (ESI) m/e 739.2 [(M+H) + , caled for ( ' n·1 I U I O XKOXS 739.1], tR = 1.90 min (Method 1).

7 Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy~6-(((3R,4R,5S)~5-(4-(5- fluoropyrimidin-2-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-277-pyran-3 -yl)thio)-5 - methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triazol-l-yl) tetrahydro-2//-pyran-2- yl)methyl acetate (28 mg, 0.038 mmol) in THF (0.7 mL) and MeOH (0.7 mL) at -10 °C was added sodium methoxide (25% wt solution in methanol) (0.043 mL, 0.190 mmol). The reaction mixture was stirred at -10 °C for 1 h. The mixture was neutralized until slightly acidic by the addition of 1 N HC1 (0.35 mL). The mixture was concentrated and was taken up in dioxane/m ethanol/water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(5- fluoropyrimi din-2 -yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-27/-pyran-3 -yl)thio)-2- (hydroxymethyl)-5-methoxy-4-(4~(3, 4, 5-tri fluorophenyl)- 1/7- 1,2, 3~triazol~l~

yl)tetrahydro-2i/-pyran-3-ol (20.3 mg, 0.031 mmol, 81% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 8 98 (s, 2H), 8 96 (s, i l l major), 8.94 (s, H i, minor), 8.86 (s, 1 H), 7.88 - 7.80 (m, 2H), 5 15 (dd, ./ 10.4, 2.9 Hz, 1H, minor), 4 98 (dd,

, 7=10.5, 2.9 Hz, 1H), 4.84 (d, J= 9.3 Hz, 1H), 4.70 - 4.63 (m, 2H), 4.52 (br d, ,7=2.6 Hz, 1H, minor), 4.23 (br dd, ,7=1 1.9, 5.0 Hz, 2H), 4.15 - 4.08 (m, 2H), 4 03 - 3.93 (m, 3H), 3.91 - 3.83 (m, 1H), 3.80 (t, .7=6.3 Hz, 1H), 3.58 - 3.51 (m, 3H), 3.25 (s, 3H, major), 3.29 - 3 23 (m, 1 H), 3.22 (s, 3H, minor); LC/MS (ESI) mie 655.2 | (M I I ) . calcd for

C26H27F4N8O6S 655.2], /s = 1.78 min (Method 1); HPLC (Method 1): t& = 4.84 min; HPLC (Method 2): fe = 4.82 min. hGal-3 ICso = 0.009 mM

Example 8. (2R, 3 R,4S,5R, 6S)-6-(((3R,4R, 5 S)-4-hydroxy-5-(4-(5-methoxypyrimi din-2- yl)-l/7-l ,2,3~triazol~l-yl)tetrahydro~27:/-pyran~3-yl)thio)~2-(hydrox yrnethyl)-5~methoxy~ 4-(4-(3 ,4, 5 -trifl uorophenyl)- 17/- 1 , 2,3 -triazol - 1 -y 1 jtetrahy dro-2//-pyran -3 -ol \ Ό

Example 8

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4-(5 - methoxypyrimi din-2-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2i/-pyran-3 -yl)thio)-5- methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 2, 3-triazol-l-yl)tetrahydro-2/:/-pyran-2~ yl)m ethyl acetate

(+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2i7~py ran~3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol - 1 - yl)tetrahydro-2i/-pyran-2-yl)methyl acetate (25.8 mg, 0.042 mmol) and 2-ethynyl-5- methoxypyrimidine (11.23 mg, 0 084 mmol) were dissolved in a previously degassed solution of DMF (0.75 mL) and water (0.25 niL) and the mixture was placed under argon. Sodium ascorbate (8 29 mg, 0.042 mmol) and copper(II) sulfate pentahydrate (8 36 mg, 0.033 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The solid clinging to the stir bar was dissolved by the addition of dichloromethane and methanol. The mixture was transferred to a separatory ' funnel containing water (5 mL) and saturated aqueous sodium

bicarbonate (5 L) resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (10 L). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (50% ethyl acetate containing 5% methanol/50% hexanes -- 100% ethyl acetate containing 5% methanol; 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4 -(5-methoxypyrimidin-2- yl)-li7-l ,2,3-triazol-l-yJ)tetrahydro-2/ -pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5- trifluorophenyl)- \H~\ ,2,3 -triazol- 1 ~yl)tetrahydro-2i7-pyran-2~yl)methyl acetate (25.3 mg, 0.034 mmol, 81% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 8.50 (s, 2H), 8.32 (s, 1H), 7.85 (s, 1H), 7.48 (dd, .7=8.2, 6.4 Hz, 2H), 5.52 (d, ,7=2.8 Hz, 1 H), 4.74 - 4.66 (m, 2H), 4.61 - 4.52 (m, 1H), 4.40 (dd, 7=11.7, 4 9 Hz, 1H), 4.34 - 4 20 (m, 3H), 4.19 - 4.05 (m, 5H), 4.00 (s, 3H), 3.52 (t, 7=11.9 Hz, 1H), 3.36 (s, 3H), 3.18

(ddd, 7=11.7, 9.7, 5.1 Hz, Hi), 2.1 1 (s, 3H), 1.95 (s, 3H); LC/MS (ESI) mie 751.2

[ (M i l) calcd for C31H34F3N8O9S 751.2], /R = 1.90 min (Method 1).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-

(4-(5-methoxypyrimidin-2-yl)- 177-1 , 2,3-triazol-l-yi )tetrahydro-27/-pyran-3-yi )thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l7/-l,2,3-triazol-l-yl) tetrahydro-27/-pyran-2- yl)methyl acetate (25.8 mg, 0.034 mmol) in THF (0.7 mL) and MeOH (0.7 rnL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.039 mL, 0.172 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture w¾s partially neutralized by the addition of 1 N HC1 (0.1 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent ¾s evaporated on the rotovapor and the aqueous mixture 'as lyophilized to afford (2R,3R,4S,5R,6S)-6- (((3R,4R,5S)-4-hydroxy-5-(4-(5-methoxypyrimidin-2-yl)-l 7/-l ,2,3-triazol-l- yl)tetrahydro-277-pyran-3-yl)thio)-2-(hydroxymethyl)-5-metho xy-4-(4-(3,4,5- trifluorophenyl)-li7-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-oi (22 8 mg, 0.034 mmol, 98% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 8.96 (s, 1H), 8.77 (br s, III, major), 8.94 (s, 1H, minor), 8.77 (br s, 1H), 8.64 (br s, 21 1 ), 7.85 (dd, ,7=8.9, 6.8 Hz, 2H), 5.15 (br dd, 7=10.2, 2.9 Hz, 1H, minor), 4.98 (dd, .7=10 6, 3.0 Hz,

1 1 1 ), 4.84 (d, 7=9.3 Hz, H I ), 4.71 - 4.61 (m, 21 1 ). 4 52 (br d, 7=3 2 Hz, 1 1 1, minor), 4.26 - 4.19 (m, 21 1 ), 4.11 (br dd, .7=10 8, 4.8 Hz, 2H), 4.04 - 3.93 (m, 31 1). 3.89 - 3.82 (m, 1H), 3.80 (t, .7=6 4 Hz, 1H), 3.58 - 3.51 (m, 3H), 3.25 (s, 3H, major), 3.27 (br d, 7=4.7 Hz, 1 1 1 ), 3.22 (s. 1 1 1. minor); Li ' /MS (ESI) m/e 667 2 [ {M 1 1 ) , calcd for C27H30F3N8O7S 667.2], fe = 1.79 min (Method 1); HPLC (Method 1): & = 5.74 min; HPLC (Method 2): IK = 5.44 min. hGal-3 ICso = 0.012 uM

Example 9. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2- methoxypyrimidin-5-yl)-l//-l,2,3-triazol-l-yl)tetrahydro-2Z/ -pyran-3-yl)thio)-2- (hydroxymethy 1 )-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro~2/:/-pyran~3-ol

Example 9

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2 - methoxypyrimidin-5-yl)-l//-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3-yl)thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l7/-l,2,3-triazol-l-yl) tetrahydro-2/7-pyran-2- yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2 /-pyran-2-yl)methyl acetate (18 mg, 0 029 rnmol) and 5-ethynyl-2- methoxypyrimidine (11 75 mg, 0.088 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (6.94 mg, 0.035 mmol) and copper(II) sulfate pentahydrate (10.20 mg, 0 041 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichlorom ethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of ( ' elite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separately funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over

MgSOy filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% methanol/60% hexanes 80% ethyl acetate containing 5% methanol/20% hexanes; 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4 -(2-methoxypyrimi din-5- yl)-l7/-l,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-5 -methoxy-4-(4-(3,4,5- trifluorophenyl)-l -l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-2-yl)methyl acetate (18 mg, 0.024 mmol, 82% yield) as a white solid. ¾ NMR (400 MHz, CHLOROF ORM-d) d 8.96 (s, 2H), 7.98 (s, H i), 7.84 (s, H i), 7.47 (dd, ./ 8 0, 6 5 Hz, 2H), 5.52 (d, ,7-3.0 Hz, 1H), 4.72 - 4.66 (m, 2H), 4.54 (td, ./ 10.3, 4.8 Hz, 1H), 4.39 (dd, J=11.4, 4.9 Hz, 1H), 4.34 - 4.29 (m, 1H), 4 31 - 4 22 (m, 2H), 4.19 - 4.1 1 (m, 4H), 4 09 (s, 3H), 3 59 - 3.49

(m, 1H), 3.35 (s, 3H), 3.20 - 3.11 (m, 1H), 2.10 (s, 3H), 1.95 (s, 3H); LC/MS (ESI) m!e 751.2 [(M+H) + , ea!cd for CsiHriFsNsCLS 751.2], fe = 2.05 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-

(4-(2-methoxypyrimidin-5-yl)-li/-l,2,3-triazol-l-yl)tetra hydro-2/7-pyran-3-yl)thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l7/-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-2- yl)methyl acetate (18 mg, 0.024 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wi solution in methanol) (0.027 mL, 0.120 mmol). The cooling bath w ? as removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and w¾s filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2-methoxy pyrimidin-5-yl)-l/7-l,2,3- triazol-l-yl)tetrahydro-2fl r -pyran-3-yl)thio)-2-(hydroxymethyl)-5-methoxy-4-(4-(3, 4,5- trifluorophenyl)-lZ7-l ,2,3-triazol-l-yl)tetrahydro-2Z7-pyran-3-ol (12.0 mg, 0.015 mmol, 62% yield) as a white amorphous solid. ¾ NMR (400 MHz, DMSO-de) d 9.03 (s, 2H),

8.98 (s, IB), 8.76 (s, IB), 7.86 (dd, .7=8.8, 7.0 Hz, 2H), 5.72 (br s, 1H), 5.49 (br s, 1 1 1).

4.98 (dd, .7=10.4, 2.6 Hz, 1H), 4.84 (d, ,7=9.3 Hz, 1H), 4.62 (td, .7=10.4, 4.9 Hz, 1H), 4.26 (br dd, ,7=1 1.4, 4.9 Hz, 1 ! ! ). 4 14 (br dd, 7=10.9, 4 6 Hz, 1H), 3.98 (s, 31 i ), 3.95 (br s, 2H), 3.86 - 3.78 (m, 2H), 3.54 (br t, ,7=1 1.4 Hz, 4H), 3.30 (br dd, ,7=11.0, 5.0 Hz, 1H),

3.25 (s, 31 1 }. LC/MS (ESI) m/e 667.2 [(M+H) + , calcd for C27H29F3N8O7S 667 2], fo ! .92 min (Method 2); HPLC (Method 3): hi = 8.65 min; HPLC (Method 4): t& = 7.44 min. hGal-3 IC50 = 0.012 mM. Example 10. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(3- hydroxyphenyl)~l/:/-l ,2,3-triazol-l-yl)tetrahydro-2/:/-pyran-3-yl)thio)-2- (hydroxymethyl)-5-methoxy-4-(4-(3 , 4, 5-tri fluorophenyl)- 177-1, 2, 3-tiiazol-l- yl)tetrahydro-2/7-pyran-3-ol

Example 10 Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4-(3 - hy droxypheny !)- IH~ 1,2,3 -tri azol- 1 ~yl)tetrahydro-2/7-pyran-3 -yl)thi o)-5~methoxy~4~(4- (3 ,4, 5-trifluoropheny!)- 1 H- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2/7-pyran-2-yl)methyi acetate (+)-(C2R,3R,4S,5R,6S)~3-Acetoxy-6-(((3R,4R,5S)-5-azido~4-hyd roxytetrahydro~ 2//-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1,2,3 -triazol- 1 - yl)tetrahydro~2/:/~pyran~2-yl)methyl acetate (18 mg, 0 029 mmol) and 3-ethynylphenol (10.35 mg, 0.088 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 niL) and the mixture was placed under argon. Sodium ascorbate (6 94 mg, 0.035 mmol) and copper(II) sulfate pentahydrate (10 20 mg, 0.041 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Ceiite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 ml.) w'as added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% ethanol/60% hexanes 80% ethyl acetate containing 5%

methanol/20% hexanes; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6- i((3R,4R, 5 S)-4-hydroxy-5-(4-(3 -hy droxyphenyl)- !//- 1 ,2,3 -tri azol- 1 ~yl)tetrahydro-2/:/- pyran-3 -y 1 )thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -tri azol- 1 - yl)tetrahydro~2/:/~pyran~2-yl)methyl acetate (15 mg, 0 020 mmol, 70% yield) as a white solid. 4 1 NMR (400 MHz, Acetone) d 8 81 (s, 1 ! ! }. 8 42 (s, 1 ! ! }. 7 74 (dd, 7=9.0, 6.8 Hz, 2H), 7.49 - 7.42 (m, 1H), 7.38 - 7.34 (m, 1H), 7.30 - 7.22 (m, 1H), 6.83 (ddd, 7=8.0, 2.5, 1.0 Hz, 1 H), 5.59 (d, 7=2.3 Hz, 1 1 1 ), 5.26 (dd, 7=40.5, 3.0 Hz, 1 1 1 ), 5.06 (d, 7=9.5 Hz, 1H), 4.75 - 4.61 (m, H i), 4.44 (t, 7=6.7 Hz, 1H), 4.32 - 4.12 (m, 7H), 3.98 (t, 7=11.2 Hz, 1 1 1 ).. 3.64 (t, 7=11.7 Hz, 1 1 1 ), 3.36 (s, 3H), 2.10 (s, 3H), 1.99 (s, 31 1 ). LC/MS (ESI) m/e 735.3 [(M+H) + , calcd for C32H34F3N6O9S 735.2], fe = 2.03 min (Method 2). Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy~6-(((3R,4R,5S)~4-hydroxy-5- (4-(3 -hydroxyphenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-3 -yl)thio)-5 -m ethoxy - 4-(4-(3 ,4, 5-trifluorophenyl)- 1/7- 1 ,2,3 -triazol - 1 -y l)tetrahydro-2//-pyran-2-y Ijmethyl acetate (15 mg, 0.020 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.023 mL, 0.102 mmol). The cooling bath was removed and the reaction mixture was stirred at it for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was

concentrated and was taken up in dioxane/m ethanol /water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6- (((3R,4R, 5 S)-4-hydroxy-5-(4-(3 -hydroxyphenyl)- 1 // 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7- pyran-3-yl)thi o)-2-(hydroxymethyl)-5-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- \H-\, 2, 3- triazol-l-yl)tetrahydro-2//-pyran-3-ol (1 1.4 mg, 0.017 mmol, 84% yield) as a white amorphous solid. 4 1 NMR (400 MHz, DMSO-de) d 9 59 (hr s, H i), 8.98 (s, H I ), 8.60 (s, 1 1 1 ), 7.86 (dd, .7=8.9, 6.9 Hz, 2! ! ). 7 28 (d, .7=1.0 Hz, H i ), 7.26 - 7 21 (m, 2H), 6.77 - 6.70 (m, 1H), 5.79 - 5.61 (m, H i). 5.56 - 5.43 (m, 1H), 4.98 (dd, .7=10.4, 2.9 Hz, 1H), 4.84 (d, .7=9 3 Hz, 1 H), 4.64 - 4.50 (m, 1H), 4 29 - 4.20 (m, 1H), 4.1 1 (br dd, ,7=10.9, 4.9 Hz, 1H), 4.02 - 3.92 (m, 3H), 3.85 - 3.76 (m, 2H), 3.56 - 3.51 (m, 2H), 3.26 (s, 3H); LC/MS (ESI) mie 651 .2 [(M+H) + , calcd for t%[ i M=\TO-S 651.2], / >, = 1.92 min

(Method 2); HPLC (Method 3): t& = 9.11 min; HPLC (Method 4): ίk = 8.16 min. hGal-3 ICso = 0.038 uM.

Example 11. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(2-chloropyridin-4- yl)-l//-l ,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3-yl)thio)- 2-(hydroxymethyl)- 5-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 1/7-1, 2, 3-triazol-l -yl)tetrahydro-2/7-pyran-3-ol

Example 11

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(2- chloropyridin-4-yl)-l/7-I,2,3-triazol-l-yl)-4-hydroxytetrahy dro-2 /-pyran-3-yl)thio)-5- methoxy-4-(4-(3,4,5-tiifluorophenyl)-l//-l,2,3-triazol-l-yl) tetrahydro-2i/-pyran-2- yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)- l//-l,2,3-triazol-l- yl)tetrahydro-2i/-pyran-2-yl)methyl acetate (20 mg, 0.032 mmol) and 2-chloro-4- ethynylpyridine (13.39 mg, 0.097 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (7.71 mg, 0.039 mmol) and copper(II) sulfate pentahydrate (1 1.34 mg, 0 045 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichlorom ethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of ( ' elite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted with 5% methanol in dichloromethane (3 x 10 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4- (2-chloropyridin-4-yl)- 177-1, 2, 3-triazol-l-yl)-4-hydroxytetrahydro~2/7-pyran~3~yl)thio)~ 5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l/7-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-2- yl)methyl acetate (23 mg, 0.030 mmol, 94% yield) Ή NMR (400 MHz,

CHLOROFORMS) d 8.45 (d, .7=5.0 Hz, 1H), 8.1 1 (s, 1H), 7.85 (s, 1H), 7.78 (s, 1H), 7.67 (dd, .7=5 3, 1.3 Hz, 1H), 7.46 (dd, .7=7.8, 6.5 Hz, 2H), 5.52 (d, 7=3.0 Hz, 1H), 4.73 - 4.65 (m, 2H), 4.57 - 4.49 (m, H i ), 4.40 (br dd, .7=11 5, 4 8 Hz, I I I ). 4.35 - 4.29 (m, 2! ! ). 4.29 - 4.22 (m, 2H), 4.17 - 4.10 (m, 2H), 4.09 - 4.02 (m, 1H), 3.52 (t, J=11.9 Hz, 1H),

3.35 (s, 3H), 3.15 (ddd, ,7=1 1.7, 10.0, 5.1 Hz, l i l), 2.10 (s, 3H), 1.94 (s, 3H); LC/MS (ESI) m!e 754.2 [(M+H) + , calcd for C31H32CIF3N7O8S 754.2], ίk = 1.91 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(2- chloropyridin-4-yl)-l /7-I ,2,3-triazol-l-yl)-4-hydroxytetrahydro-277-pyran-3-yl)thio)- 5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l77-l,2,3-triazol-l-yl) tetrahydro-27/-pyran-2- yl)methyl acetate (23 mg, 0.030 mmol) in THF (0.5 rnL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.035 mL, 0.152 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(2-chloropyridin-4-y l)-17/-l ,2,3-triazol-l-yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-2-(hydroxymethyl)-5-m ethoxy-4-(4-(3,4,5- trifluorophenyl)-l 77-l ,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3-ol · TFA (l4. l mg, 0.017 mmol, 57% yield) as a white amorphous solid. l H NMR (400 MHz, DMSQ-dcf) d 9.03 (s, H i), 8.98 (s. H i), 8.50 (d, J= 5 0 Hz, H i), 7.93 (s, 1H), 7.89 - 7.81 (nr. 3H), 5 74 (hr dd, ,7=4.5, 1.5 Hz, l i l). 5.50 (hr d, .7=5.5 Hz, 1H), 4.98 (dd, 7=10.5, 2.8 Hz, 1H), 4.84 (d, J= 9.3 Hz, H i), 4.62 (id, ./ 10.4, 4.9 Hz, l i l ), 4.26 (br dd, ./ i i .7, 4.6 Hz, 1H), 4 16 (dd, .7=10.8, 4.8 Hz, 1H), 4.02 - 3.90 (m, 31 1). 3.85 - 3.76 (m, 2H), 3.57 - 3.51 (m, 4H), 3.34 - 3.27 (m, 1H), 3.25 (s, 3H); LC/MS (ESI) mie 670.1 [(M+H) + , caicd for C27H28CIF3N7O6S 670.1], /:< 1.80 min (Method 2), i iPi .C (Method 3): /¾ = 9.75 min;

HPLC (Method 4): /R = 8.66 min. liGal-3 IC50 = 0.037 mM.

Example 12. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-phenyl- 1 if- 1,2, 3 -tri azol- 1 -yl)tetrahy dro-2f/-pyran-3 -yl)thi o)-2-(hy droxymethyl)-5 -methoxy-4- (4-(3, 4, 5-tri fluorophenyl)-!/:/- 1 ,2, 3-triazol-l-yl)tetrahydro-2i/-pyran-3-ol

Example 12

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4- phenyl-li/-l,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio )-5-methoxy-4-(4-(3,4,5- tri fl uoroph eny 1) - 1 H- 1 , 2 , 3 -tri azol - 1 -y 1 )t etrahy dro-2f/~py ran -2-y 1 )m ethyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)- l ii-l ,2,3-triazol-l- yi)tetrahydro-2/f-pyran-2-yi)methyl acetate (24 mg, 0.039 mmol) and ethynylbenzene (11.93 mg, 0.117 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (9.25 mg, 0.047 mmol) and copper(II) sulfate pentahydrate (13.61 mg, 0.054 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel . Brine (1(3 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol;

12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4- phenyl-li7-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-5-methoxy- 4-(4-(3,4,5- trifluorophenyl)-li/-l,2,3-triazol-l-yl)tetrahydro-2/ -pyran-2-yl)methyl acetate (11 mg, 0.(315 mmol, 39% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.92 (s, 1H), 7.86 - 7.80 (m, 3H), 7.50 - 7.41 (m, 4H), 7.40 - 7.33 (m, 1H), 5.51 (d, =3.0 Hz, i l l ). 4 74 - 4.65 (m, 2H), 4.57 - 4.48 (m, H i ).. 4.38 (dd, ./ 1 1 .5, 4.8 Hz, H i ).. 4.34 - 4.03 (m, 7H), 3.53 (t, =11.9 Hz, 1H), 3.36 (s, 3H), 3.18 (ddd, J=11.9, 9.5, 5.0 Hz, 1H), 2.10 (s, 31 13, 1.93 (s, 31 13: LC/MS (ESI) mle 719.3 [(M+H) + , calcd for G ;.Ί I O F -NI.OKS

719.2], hi = 2.01 min (Method 2).

Step 2

To a suspension of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-(4-phenyl-l/ - 1 ,2,3 -tri azol- 1 -y !)tetrahydro~27/~pyran~3 -yl)thio)-2-(acetoxy methy l)-5-m ethoxy-4-(4-

(3,4,5-trifluorophenyl)-li7-l,2,3-triazoJ-l-yl)tetrahydro -2i/-pyran-3-yJ acetate (11 mg, 0.014 mol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.017 mL, 0.072 mmol). The cooling bath was removed and the reaction mixture was stirred at it for 1 h. The mixture was partially neutrali zed by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy- 5-(4-phenyl- 177-1,2, 3-tri azol- l-yl)tetrahydro-2//-pyran-3-yl)thio)-2-(hydroxymethyl)-5- m ethoxy -4-(4-(3 ,4,5-trifluorophenyJ)- IH- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2i/-pyran-3 -ol

(8.1 mg, 0.012 mmol, 81% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 8.98 (s, IH), 8.68 (s, IH), 7.89 - 7.82 (m, 4H), 7.47 (t, .7=7.7 Hz, 2H), 7.40 - 7.32 (m, 1H), 5.72 - 5.66 (m, 1H), 5.49 (br d, ,7=5.5 Hz, 1H), 4.98 (dd, ,7=10.5, 2.9 Hz, 1 1 1 ), 4.84 (d, ,/ 9.2 Hz, i l l), 4 63 - 4 55 (m, 1 H), 4.25 (dd, .7=11 5, 5.0 Hz, I I I ), 4.12 (dd ,7=10.7, 4.7 Hz, 1H), 4.01 - 3.92 (m, 3H), 3.84 - 3.76 (m, 2H), 3.56 - 3.49 (m, 3H), 3.28 (br dd, ./ 1 1 .0, 4.4 Hz, 1H), 3 25 (s, 31 1 ): LC/MS (ESI) m/e 635.2 | (\i · H) . calcd for C sHsoF-iNeQeS 635.2], ίk = 1.88 min (Method 2); HPLC (Method 3): & = 13.65 min; HPLC (Method 4): /¾ = 11.47 min. hGal-3 ICso = 0 032 uM

Example 13. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-aminophenyl)-177- l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2 /-pyran-3-yl)thio)-2-(hydroxymethyl)-5- m 4-(4-(3,4,5-trifluorophenyl)-I /7-l,2,3-triazol-l -yl)tetrahydro-277-pyran-3-ol

NH,

Example 13

Step 1 Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3- aminophenyl)- 177-1 , 2,3 -triazol- 1 -yl)-4-hy droxytetrahy dro-277-pyran-3 -yl)thi o)-5 - methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triazol-l-yl) tetrahydro-27/-pyran-2- yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)- l /7-I ,2,3-triazol-l- yl)tetrahydro-277-pyran-2-yl)methyl acetate (24 mg, 0.039 mmol) and 3-ethynylaniline (13 68 mg, 0.1 17 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (9.25 mg, 0.047 mmol) and copper(II) sulfate pentahydrate (13.61 mg, 0.054 mmol) (predissolved in 0 3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in diehoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 5 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol, 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-

(3 -aminopheny 1)- 1 H- 1 ,2,3 -tri azol- 1 ~yl)~4-hydroxy te†rahydro-277-pyran-3 ~yl)thio)~5 - methoxy-4-(4-(3,4,5-trifluorophenyl)-17/-l,2,3-triazol-l-yl) tetrahydro-27/-pyran-2- yl)methyl acetate (25 mg, 0.034 mmol, 88% yield) as a white solid. ¾ NMR (400 MHz, CHLOROF ORM-d) d 7.87 (s, 1H), 7.83 (s, 1H), 7.47 (dd, ,7=8.0, 6.5 Hz, 2H), 7.28 - 7.19 (m, 3H), 7.12 (d, ,/ 7.5 Hz, 1H), 6 77 - 6.65 (m, I I I). 5.50 (d, ,7=3.0 Hz, i l l ), 4.72 - 4.65

(m, 2H), 4.55 - 4.47 (m, 1H), 4.37 (dd, 7=11.5, 4.8 Hz, 1H), 4.32 - 4.25 (m, 2H), 4.22 (br d, 7=9.0 Hz, i f If 4 19 - 4.11 (m, 21 1), 4.09 - 4.00 (m, 21 If 3.53 (t, 7=11 9 Hz, 1 1 1), 3.35 (s, 3H), 3.22 - 3.12 (m, 2H), 2.10 (s, 3H), 1.93 (s, 4H); LC/MS (ESI) mie 734.2 [(M+H) + , calcd for C32H35F3N7O8S 734.2], ts. = 1.79 min (Method 2).

To a suspension of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-acetoxy-5-(4-(3- aminophenyl)- 177- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-277-pyran-3-yl)thio)-2-(acetoxymethyl)- 5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 177- 1,2, 3-tri azol- l-yl)tetrahydro-27/-pyran-3-yl acetate (25 mg, 0.032 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.037 mL, 0.161 mmol). The cooling bath was removed and the reaction mixture w¾s stirred at it for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methano!/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6- (((3R,4R,5S)-5-(4-(3-aminophenyl)-li/-l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2i7- pyran-3~yl)thi o)-2~(hydroxymet.hyi)-5-Tnethoxy-4-(4~(3, 4, 5-tri fluorophenyl)-!//- 1,2,3- triazol-l-yl)tetrahydro-2//-pyran-3-ol (22.5 mg, 0.028 mmol, 87% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 8.98 (s, 1H), 8.66 (s, 1H), 7.86 (dd, J= 8 7, 6 9 Hz, 2H), 7.57 (br s, 1 1 1), 7.45 (br d, ,/ 7.2 Hz, 1H), 7.41 - 7.34 (m, 1 1 1 ). 6.99 (br d, J=7.5 Hz, 1H), 4.98 (dd, J=10.5, 2.7 Hz, 1H), 4.84 (d, J=93 Hz, 1H), 4.59 (td,

,/ 10.3, 5.0 Hz, 1 H), 4.28 - 4.20 (m, i l l), 4.12 (br dd, ./ 1 1 . 1 , 4 8 Hz, 1 1 1 ). 4.00 - 3.92 (m, 3H), 3.84 - 3.77 (m, 2H), 3.57 - 3.49 (m, 3H), 3.30 - 3.26 (m, 1H), 3.25 (s, 3H); LC/MS (ESI) m/e 650 2 [(M+H) + , calcd for CisIluFsNvOeS 650.2], IR = 1.65 min (Method 2); HPLC (Method 3): ίk = 9.63 min; HPLC (Method 4): /s = 7.35 min. hGal-3 ICso = 0 022 mM.

Example 14. Preparation of 7V-(3-(l-((3S,4R,5R)-4-hydroxy-5-(((2S,3R,4S,5R,6R)-5- hy droxy-6-(hydroxymethyl)-3 -methoxy-4-(4-(3 ,4,5-trifluorophenyl)- 1 H- 1 ,2, 3 -triazol- 1 - y 1 Hetrahy dro-2//-pyran-2-y 1 )thio)tetrahydro-2i7-pyran-3 ~yl)~ 1 H- 1 , 2,3 -triazol -4- yl)phenyl)acetamide

O

/

Example 14

Step 1. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-acetamidophenyl)-li7 - l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2 /-pyran-3-yl)thio)-2-(acetoxymethyl)-5- m ethoxy -4-(4-(3 ,4,5-trifluorophenyl)- IH- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2f/-pyran-3 -yl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2i/-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -tri azol- 1 - yl)tetrahydro-2 /-pyran-2-yl)methyl acetate (24 mg, 0 039 mmol) and A ? -(3- ethynylphenyl)acetamide (18 59 mg, 0.117 mmol) were dissolved in a previously degassed solution of DMF (0.9 ml) and water (0.300 ml.) and the mixture was placed under argon. Sodium ascorbate (9 25 mg, 0.047 mmol) and copper(II) sulfate pentahydrate (13.61 mg, 0 054 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichorom ethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The separately funnel containing the brine was rinsed with di chi orom ethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol; 24 g column) to afford (2R,3R,4S,5R,6S)-6- (((3R,4R,5S)-5-(4-(3-acetamidophenyl)-li7-l,2,3-triazol-l-yi )-4-hydroxytetrahydro-2//- pyran-3 -yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1/7- 1,2,3- triazo!~l-yl)tetrahydro-2i7~pyran-3~yl acetate (28 mg, 0.036 mmol, 93% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 7.96 - 7.83 (m, 3H), 7.56 (br d, 7=7.8 Hz, 1 1 1 ). 7.45 (br dd, 7 13.3, 6.3 Hz, 3H), 7.37 - 7.31 (m, i l l), 5.51 (d, 7=2 5 Hz, 1 1 1 ). 4.89 (br s, 1H), 4.73 (br d, 7=9.8 Hz, 2H), 4.54 - 4.45 (m, 1H), 4.38 - 4.26 (m, 4H), 4.23 - 4.10 (m, 2H), 4.08 - 4.00 (m, 1H), 3.56 (br t, 7=1 1.9 Hz, i l l ). 3 35 (s, 31 1 ). 3 21 (td, 7=10.8, 5.0 Hz, 1H), 2.23 (s, 3H), 2.1 1 (s, 3H), 1.96 (s, 3H); LC/MS (ESI) mie 776.3 [(M+Hr, ca!cd tor C34H37F3N7Q9S 776.2], fe = 1.92 min (Method 2).

Step 2

To a suspension of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-acetamidophenyl)- IHA ,2, 3 -triazol- 1 -yl)-4-acetoxytetrahydro-2/ -pyran-3 -yl)thio)-2-(acetoxymethyl)-5- methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 177-1,2, 3-triazol-l -yl)tetrahydro-2//-pyran-3-yl acetate (28 mg, 0.034 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.039 mL, 0.171 mmol). The cooling bath was removed and the reaction mixture was stirred at it for 1 h. The mixture was partially neutralized by the addition of l N HC1 (0. 18 mL). The mixture was

concentrated and rvas taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford /V-(3-(l -((3S,4R,5R)-4- hydroxy-5-(((2S,3R,4S,5R,6R)-5-hydroxy-6-(hydroxymethyl)-3-m ethoxy-4-(4-(3,4,5- trifluorophenyl)- 1//-Ί ,2,3-triazol- 1 ~yl)tetrahydro-2if-pyran-2~yl)thio)tetrahydro-2//~ pyran-3-yl)-li7-l ,2,3-triazol-4-yJ)phenyl)acetamide (24.1 mg, 0.028 mmol, 82% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 10.06 (s, 1H), 8.98 (s, 1H), 8.62 (s, 1H), 8.16 (s, 1H), 7.86 (dd, .7=8.9, 6.8 Hz, 21 1). 7.54 (br d, 7=8.2 Hz, 1H), 7.45 (d, J=7.8 Hz, 1H), 7.41 - 7.33 (m, 1H), 4.98 (dd, .7=10.6, 2.8 Hz, 1H), 4.83 (d, .7=9.3 Hz, I I I ). 4.63 - 4.56 (m, 1 1 1 ). 4.29 - 4.20 (m, 1H), 4.1 1 (br dd, J= 1 1.1 , 4.9 Hz, 1 H), 4.01 - 3.92 (m, 3H), 3.86 - 3.77 (m, 2H), 3.59 - 3.49 (m, 3H), 3.31 - 3.26 (m, 1H), 3.25 (s,

3H), 2.07 (s, 3H), LC/MS (ESI) m/e 692 2 [(M+H) + , calcd for C30H33F3N7O7S 692.2], IR = 1.73 min (Method 2); HPLC (Method 3): /s = 13.31 min; HPLC (Method 4): 2 = 1 1 .65 min. hGal-3 IC50 ::: 0.020 mM.

Example 15. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4- (pyrirnidin-5-yi)-!i/~l,2,3-triazol-l~yl)tetrahydro-2/ -pyran-3~yl)thio)-2~

(hydroxymethyl)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l -l,2,3-triazol-l - yl)tetrahydro-2//-pyran-3 ~ol

Example 15

Step 1. Preparation of (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-( 4- (pyrimidin-5-yl)-l7/-l,2,3-triazol-l-yl)tetrahydro-2//-pyran -3-yl)thio)-5-methoxy-4-(4- (3,4,5-trifluorophenyl)-l77-l,2,3-triazol-l-yl)tetrahydro-27 7-pyran-2-yl)methyl acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2H-py ran-3 -yl)thio)~5-methoxy~4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol - 1 - yl)tetrahydro-2i/-pyran-2-yl)methyl acetate (27 mg, 0.044 mmol) and 5- ethynylpyrimidine (13.68 rng, 0.131 mmol) were dissolved in a previously degassed solution of DMF (0.9 niL) and water (0.300 niL) and the mixture was placed under argon. Sodium ascorbate (8.68 mg, 0.044 mmol) and copper(II) sulfate pentahydrate (7.65 mg, 0 031 mmol) (predissolved in 0.2 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The reaction mixture was filtered through a pad of ( ' elite and the filtrate was transferred to a separatory funnel containing saturated aqueous NaHCCb solution (10 mL). The aqueous layer was extracted with dichloromethane (3 x 15 mL). The combined organic layers were washed with brine (10 mL), dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (10% > 100% ethyl acetate in hexanes; 12 g column) to afford (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-( 4-(pyrimidin-5- yl)-l7r-l,2,3-triazoi-l-yl)tetrahydro-2/7-pyran-3-yi)thio)-5 -methoxy-4-(4-(3,4,5- trifluorophenyl //~l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-2-yl)methyl acetate (12 mg, 0.017 mmol, 38% yield) as a white solid. [CC]D 2 +72.0, (c = 0.320, CHCb); J H NMR (400 MHz, CHLOROF ORM-d) d 9.23 (s, 1H), 9.20 (s, 2H), 8.10 (s, 1H), 7.83 (s, IH), 7.47 (dd, 7=8.2, 6.4 Hz, 21 1 ).. 5.52 (d, 7=3.0 Hz, i l l ). 4/72 - 4 63 (m, 21 1 ), 4.60 - 4.51 (m, 1 1 1 ), 4.41 (dd, 7=1 1.7, 4.9 Hz, 1 1 1 ), 4.32 (dd, 7=12.2, 5.1 Hz, 1H), 4.27 (t, 7=9.9 Hz, 1H),

4.21 - 4 03 (m, 41 1 ), 3.58 - 3.48 (m, i l l ). 3 36 (s, 3H), 3 15 (ddd, 7=12.0, 9.9, 5.0 Hz,

1 1 1 ). 3.01 - 2.88 (m, 1 1 1 ), 2.11 (s, 31 1 ), 1.95 (s, 31 1 ); LC/MS (ESI) mie 720.4 [(M+H) + , calcd for CiioHnFsNgGsS 721.2], t& = 1.81 min (Method 2). Step 2

To a solution of (+)-((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5- (4-(pyrimidin-5-yI)-U7-l,2,3-triazol- ! -yl)tetrahydro-217-pyran-3-yl)thio)-5-methoxy-4- (4-(3,4,5-trifluorophenyl)-l /-l,2,3-triazol-l-yl)tetrahydro-2//-pyran-2-yl)methyl acetate (23 mg, 0.032 mmol) in methanol (0.5 mL) and tetrahydrofuran (0.5 mL) at 0 °C was added sodium methoxide (25% wt in MeOl I ) (0.036 mL, 0.160 mmol) . The reaction mixture was stirred at rt for 2 h. The reaction mixture was cooled to 0 °C and was partially neutralized with 1 N HC1 (0.10 mL). The volume of solvent was reduced on the rotovapor and the mixture was diluted with methanol, filtered, and purified by reverse phase preparative HPLC (Method 4). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (+)-(2R,3R,4S,5R,6S)-6- (((3R,4R,5S)-4-hydroxy-5-(4-(pyrimidin-5-yl)-li/-l,2,3-triaz ol-l -yl)tetrahydro-2//- pyran-3-yl)thi o)-2-(hydroxymethyl)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)-!//- 1,2, 3- triazol-l-yl)tetrahydro-2/-pyran-3-ol (11.4 mg, 0.018 mmol, 56% yield) as a white amorphous solid. I a jo” +64.3, (c ------ 0235, DMF); ; i! NMR (500 MHz, Acetone) d 9.24

(s, 2H), 9.13 (s, 1H), 8.77 (s, 1H), 8.73 (s.111), 7.76 (dd, .7=8.9, 6.8 Hz, 2!!}.500 (dd,

7 10.5, 2.9 Hz, 1H), 4.92 (d, J=9.3 Hz, 1H), 4.74 - 4.67 (m, 1H), 4.32 - 4.22 (m, 3H), 4.18 (t, .7=9.9 Hz, 1H), 4.08 (t, .7=10.0 Hz, 111), 4.01 - 3.92 (m, 211), 3.84 (dd, 7=5.8, 35

Hz, 211).3.62 (t, 7=11.7 Hz, 1H), 3.37 - 3.32 (m, 111).3.30 (s, 311); !3 C NMR (126 MHz, DMSO-de) d 158.1, 153.7, 1438, 1406, 125.5, 123.5, 122.9, 110.0, 1100, 109.8, 82.9, 79.6, 76.8, 72.2, 71.7, 69.2, 67.9, 66.5, 64.0, 60.7, 60.1, 55.4, 46.7; LC/MS (ESI) m/e 637.2 [(M+H) + , calcd for Cfo.f I.M+NKCES 6372], fe = 1.70 min (Method 2); HPLC

(Method 3); tn = 8.32 min; HPLC (Method 4): /R = 6.98 min. hGal-3 ICso = 0.015 mM.

Example 16. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-chlorophenyl)· l//-l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2/7-pyran-3-yl)t hio)-2-(hydroxymethyl)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l7/-l,2,3-triazol-l-yl) tetrahydro-27/-pyran-3-ol

Example 16

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3- chlorophenyl)- 1 H- 1 ,2,3 -tri azol- 1 -yl)-4-hydroxytetrahydro-277-pyran-3 -yl)thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triazol-l-yl) tetrahydro-2/-pyran-2- yl)methyl acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2/ -pyran-3-yl)thi o)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)-! 1,2, 3-triazol-l- yl)tetrahydro-2/7-pyran-2-yl)methyl acetate (18 mg, 0.029 mmol) and l-chloro-3- ethynylbenzene (11.96 mg, 0.088 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (6.94 mg, 0.035 mmol) and copper(II) sulfate pentahydrate (10.20 mg, 0.041 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (20 mL). The organic layer w'as dried over

MgS() 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (10% ethyl acetate containing 5% methanol/90% hexanes > 70% ethyl acetate containing 5% methanol/30% hexanes; 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(3-chloro phenyl)-l/7-l,2,3-triazol-l- yl)-4-hydroxytetrahydro-2//-pyran-3-yl)thio)-5-methoxy-4-(4- (3,4,5-†rifluorophenyl)- li7-l,2,3-triazol-l-yl)tetrahydro-2i/-pyran-2-yl)methyl acetate (21 mg, 0.028 mmol, 96% yield) as a white solid. 1 H NMR (400 MHz, CHLOROFORM-d) 6 7 94 (s, i l l). 7 84 (s, 1H), 7.81 (t, .7=1.6 Hz, 1H), 7.71 (dt, .7=7.5, 1.4 Hz, 11 1). 7.47 (dd, ,7=8.2, 6.4 Hz, 2H), 7.42 - 7.31 (m, 2H), 5.51 (d, .7=3 0 Hz, 1 1 1), 4.74 - 4.66 (m, 2H), 4.58 - 4.47 (m, H i), 4.38 (dd, 7=1 1.5, 4.8 Hz, 1 H), 4.34 - 4.23 (m, 3H), 4.21 - 4 12 (m, 4i I), 4.10 - 4.01 (m,

1H), 3.53 (t, 7=12.0 Hz, 1H), 3.35 (s, 3H), 3.16 (ddd, 7=12.0, 9.7, 5.1 Hz, 1H), 2.10 (s, 3H), 1.93 (s, 3H), LC/MS (ESI) m/e 753 2 [(M+H) + , catcd for C ' ui foC ' lfoMOxS 753.2], /R = 1.99 min (Method 2). Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3~aeetoxy-6-(((3R,4R,5S)-5-(4~(3- chlorophenyl)-177-l,2,3-triazol-l-yl)-4-hydroxytetrahydro-27 7-pyran-3-yl)thio)-5- m ethoxy -4-(4~(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-2- yl)m ethyl acetate (21 mg, 0 028 mmol) in THF (0 5 ml,) and MeOH (0 5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.032 mL, 0 139 mmol). The cooling bath was removed and the reaction mixture was stirred at it for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(3-chlorophenyl)-l// -l,2,3-triazol-l -yl)-4- hydroxytetrahydro-2/7-pyran-3-yl)thio)-2-(hydroxymethyl)-5-m ethoxy-4-(4-(3,4,5- trifluorophenyl)-l//-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-3-ol (15.6 mg, 0 023 mmol, 81% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSQ-de) d 8.95 (s, IH), 8.78 (s, H i), 7.89 (t, 3 1 .8 Hz, H i ).. 7.87 - 7.79 (m, 3H), 7.54 - 7 48 (m, H I ), 7.43 - 7.39 (m, IH), 4.97 (dd, 7=10.5, 2.9 Hz, IH), 4.83 (d, .7=9.3 Hz, H i). 4.63 - 4.55 (m, IH), 4.25 (dd, 7=11.7, 4.7 Hz, H i), 4.13 (dd, 7=11.1, 5 0 Hz, IH), 4.01 - 3.91 (m, 3 i I ) . 3.83 - 3.76 (m, 2H), 3.57 - 3.49 (m, 3H), 3.29 (br dd, 7=10 8, 4 7 Hz, I H), 3.25 (s, 3H); LC/MS (ESI) mie 669.1 [(M+H) + , calcd for CusHwClFsNfiOeS 669.1], ts. = 1.90 min (Method 2); HPLC (Method 3): /¾ = 15.4 min; HPLC (Method 4): fe = 14.1 min hGal-3 ICso = 0.068 mM.

Example 17. Preparation ofiV-(2-(l ~((3S,4R,5R)-4-hydroxy-5~(((2S,3R,4S,5R,6R)-5- hydroxy-6-(hydroxymethyi)-3-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 1/7- 1,2, 3-triazol-l- yl)tetrahy dro-2/7-pyran~2-yl)thio)tetrahy dro-2/7-pyran-3 ~yl)~ 1/7- 1 ,2,3 -triazol -4- yi )pyrimidin-4-yl)acetamide

Example 17

Step 1. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4-acetamidopyrimidin-2 - yl)-l//-l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3- yl)thio)-2-(acetoxymethyl)- 5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triazol-1 -yl)tetrahydro-2/7-pyran-3-yl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2/7-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2, 3 -triazol- 1 - yl)tetrahydro-2//-pyran-2-yl)methyl acetate (37 mg, 0.060 mmol) and N-(2- ethynylpyrimidin-4-yl)acetamide (22.24 mg, 0.138 mmol) were dissolved in a previously degassed solution of DMF (1.5 rnL) and water (0 500 mL) and the mixture was placed under argon. Sodium ascorbate (14.27 mg, 0.072 mmol) and eopper(II) sulfate pentahydrate (20.98 mg, 0.084 mmol) (predissolved in 0.5 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (10 mL) and saturated aqueous sodium bicarbonate (10 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separately funnel. Brine (20 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 L). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column

chromatography on silica gel (20% 100% ethyl acetate containing 5%

methanol/hexanes; 24 g column) to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4- aceiamidopyiimidin-2-yl)-li7~l,2,3-triazol-l-yl)-4-hydroxyte trahydro~2/: -pyran~3- yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5-trifluoroph enyl)-l/7-l,2,3-triazol-l - yi)tetrahydro-2/:/~pyran~3-yl acetate (35 mg, 0.045 mmol, 75% yield) as a white solid. ¾ NMR (400 MHz, DMSO-de) d 1 1.16 (s, IH), 8.99 (s, IH), 8.81 (s, IH), 8.71 (d, ,7=5.8 Hz, IH), 8.00 (d, ,7=5.8 Hz, IH), 7.89 - 7.78 (m, 2H), 5.76 (br d, 7=6.8 Hz, IH), 5.40 (d, 7=2 8 Hz, IH), 5.28 (dd, .7=10.5, 3 0 Hz, IH), 5.05 (d, 7=9.5 Hz, I H), 4.69 (td, 7=10.2, 4.9 Hz, IH), 4.35 (t, 7=6.4 Hz, IH), 4.26 - 4.13 (m, 2H), 4.11 - 3.99 (m, 4H), 3.90 (t,

,7=1 1 .2 Hz, I H), 3.59 (br t, 7=11.5 Hz, IH), 3.27 (s, 3H), 2.18 (s, 3H), 2.05 (s, 3H), 2.04 (s, 3H); LC/MS (ESI) mle 778.3 [(M+H) + , calcd for C32H35F3N9O9S 778.2], & = 1.90 min (Method 2). Step 2

To a suspension of (2R,3R.4S,5R.6S)-6-(((3R.4R,5S)-5-(4-(4- acetamidopyrimidin-2-yl)- 1 H- 1 ,2,3 -triazoi- 1 -yl)-4-hydroxytetrahydro-2//-pyran-3 - yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5-trifluoroph enyl)-l//-l,2,3-triazol-l - yl)tetrahydro-277-pyran-3-yl acetate (15 mg, 0.019 mmol) in THF (0.75 mL) and MeOH (0.75 mL) at -20 °C (acetone/dry ice bath) was added sodium methoxide (25% wt solution in methanol) (0.022 mL, 0.096 mmol). The cooling bath was removed and the reaction mixture was stirred at -20 °C for 1 h. A small amount of the aniline side product was also observed. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford A’-(2-(l -((3 S,4R,5R)-4-hydroxy-5-(((2S,3R,4S,5R,6R)-5-hydroxy- 6-(hydroxymethyl)-3 -methoxy-4-(4-(3 ,4,5-trifluorophenyl)- 1/7- 1 ,2,3 -triazoi- 1 - yl)tetrahy dro-2/:/-pyran~2-yl)thio)tetrahy dro-2//~pyran-3 ~yl)~ II - 1 ,2,3 -triazoi -4- yl)pyrimidin-4-yl)acetamide (9.8 mg, 0.012 mmol, 62% yield) as a white amorphous solid. i H NMR (500 MHz, DMSO-de) d 11.13 (s, 1 1 1 }.. 9.01 - 8.95 (m, H i), 8.79 (s, H i), 8.70 (d, 7=5.6 Hz, 1 1 1 ). 7.98 (d, ,7=5.8 Hz, IH), 7.85 (br dd, ,7=8.9, 6.9 Hz, 2H), 4.97 (dd, .7=10.7, 2.7 Hz, 1H), 4.84 (d, 7=9.3 Hz, 1H), 4.67 (td, 7=10.2, 5.0 Hz, 1H), 4.27 - 4.18 (m, 2H), 4.13 (br dd, 7=10.7, 4.7 Hz, 2H), 4 02 - 3 93 (m, 3H), 3.91 - 3.84 (m, i l l ). 3 80 (br t, 7=6.1 Hz, 1H), 3.57 - 3.49 (m, 3H), 3.25 (s, 3H), 3.29 - 3.22 (m, 1H), 2.17 (s, 3H), 1.24 (s, 1H); LC/MS (ESI) m/e 694.2 [(M+H) + , caled for C28H31F3N9O7S 694.2], &

1 .78 min (Method 2); HPLC (Method 3): 2k = 13.5 min; HPLC (Method 4): <k = 11.5 min. hGal-3 IC50 ::: 0.012 mM.

Example 18. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4-aminopyrimidin- 2-y 1)- 177- 1 ,2,3 -triazol- 1 ~yl)~4-hy droxytetrahy dro-2/7-pyran~3 -yl)thio)-2- (hydroxymethyl)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l//-l ,2,3-triazoM - yl)tetrahy dro-277-py ran-3 ~ol

Example 18

To a suspension of (2R,3R,4S,5R,6SV6-(((3R,4R,5S)~5-(4-(4~

acetamidopyrimidin-2-yl)- Iff- 1 ,2,3 -triazol- 1 -yl)-4-hy droxytetrahy dro-277-pyran-3 - yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2, 3 -triazol- 1 - yl)tetrahydro-2/7-pyran-3-yl acetate (15 mg, 0 019 mmol) in THF (0.75 mL) and MeOH (0.75 mL) at rt was added sodium methoxide (25% wt solution in methanol) (0.022 mL, 0.096 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/ water and was filtered and purified by reverse phase preparative HPLC (Method f). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4~ aminopyrimidi n-2-yl)- 1 H- 1 ,2,3 -triazol - 1 -yl)-4-hy droxytetrahy dro-2//-pyran-3 -y 1 )thio)- 2-(hydroxymethyl)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)-l77 -l,2,3-triazol-I- yl)tetrahydro-2/7-pyran-3-ol (1 1.6 mg, 0.015 mmol, 75% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 8.96 (s, 1H), 8.89 (s, 1H), 8.14 (d, J=6.9 Hz,

1 1 1 ).. 7.91 - 7.80 (m, 2H), 6.60 (br d, .7=6.6 Hz, H I ), 5.70 (hr d, 7 1 .7 Hz, H i), 5.47 (br s, 1H), 4.97 (dd, 7=10.5, 3.0 Hz, 1H), 4.84 (d, J=93 Hz, 1H), 4.78 - 4.71 (m, 1H), 4.28 - 4.20 (m, 1H), 4. 15 (br dd, J=10.9, 4.8 Hz, I I I ), 4.02 - 3.93 (m, 3H), 3 89 (br t, 7=1 1 .1 Hz, 1 1 1 ). 3.79 (br t, 7=6.4 Hz, 1H), 3.61 - 3.51 (m, 3H), 3.30 - 3.26 (m, 1H), 3.24 (s, 31 1 ): LC/MS (ESI) m/e 652 2 [(M+H) + , ea!cd for C26H29F3N9O6S 652.2], fe = 1.64 min

(Method 2); HPLC (Method 3): ίk = 12.34 min; HPLC (Method 4): ίk = 10.03 min.

h Gal-3 ICso = 0.013 mM.

Example 19. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-cyclohexyl-li7-l,2,3- triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3-yl)thio)-2-(hy droxymethyl)-5-methoxy-4- (4-(3 ,4,5-trifluoropheny !)- IH~ 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2/7-pyran-3 -ol

Example 19

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-cyclohexyl-l i - 1 , 2,3 -tri azol - 1 -yl)-4-hy droxy tetrahy dro-2/7-pyran-3 -yl)thi o)-5 -meth oxy-4-(4-(3 ,4,5- trifluorophenyl)-l -l ,2,3-triazol-l-yl)tetrahydro-2i/-pyran-2-yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3, 4, 5 -tri fluorophenyl)- 177- 1,2, 3 -tri azol- 1- yl)tetrahydro-2i7-pyran-2-yl)methyl acetate (19 mg, 0.031 mmol) and

ethynylcyciohexane (10.00 mg, 0.092 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (7.33 mg, 0 037 mmol) and copper(II) sulfate pentahydrate (10.77 mg, 0.043 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dieh!oromethane and methanol. Water (10 mL) and saturated aqueous sodium bicarbonate (10 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgSOy filtered, and concentrated. The product %vas purified by column chromatography on silica gel (20% ethyl acetate containing 5% methanol/80% hexanes— > 80% ethyl acetate containing 5% methanol/20% hexanes; 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-cyclohexy l-li7-l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2//~pyran-3-yl)thio)-5-methoxy-4~(4-(3,4,5 -trifiuorophenyl)~l/:/- l,2,3-triazol-l-yl)tetrahydro-27/-pyran-2-yl)methyl acetate (21 mg, 0.029 mmol, 94% yield) as a white solid. 41 MR (400 MHz, Acetone) d 8.78 (s, 1H), 7.78 (s, 1H), 7.73 (dd, ./ 9.0. 6 8 Hz, 21 !), 5.58 (dd, .7=3.1 , 0.9 Hz, 1 H), 5.26 (dd, ./ 10 4, 3.1 Hz, 1 H), 5.05 (d, J=9.5 Hz, 1H), 4.72 (d, .7=4.5 Hz, 1H), 4.59 - 4.50 (m, 1H), 4.46 - 4.40 (m, 1H), 4.28 - 4.19 (m, 2H), 4.17 - 4 10 (m, 4H), 3.89 (t, ./ P .3 Hz, H i ), 3 59 (t, ./ 1 1.7 Hz, 1 H),

3.35 (s, 3H), 3.32 - 3.24 (m, H i). 2.09 (s, 3H), 1.98 (s, 3H), 1.84 - 1.78 (m, 2H), 1.76 - 1.68 (m, 2H), 1.50 - 1.36 (m, 6H); LC/MS (ESI) m/e 725.3 [(M+H)L calcd for

C32H40F3N6O8S 725.3], fe = 2.08 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4- cyclohexyl-17/-1 ,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3-yl)thio)- 5-methoxy- 4-(4-(3,· 4, 5-tri fluorophenyl)- 177-1, 2, 3-triazol-l-yl)tetrahydro-27/-pyran-2-yl)methyl acetate (21 mg, 0.029 mmol) in THF (0.75 mL) and MeOH (0 75 mL) at it was added sodium methoxide (25% wt solution in methanol) (0.033 mL, 0.145 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in

dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4- cyclohexyl-li/-l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-p yran-3-yl)thio)-2- (hydroxymethyl)- 5 -methoxy-4-(4~(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - y l)tetrahy dro-2 ~py ran-3 -ol (8.5 mg, 0.013 mmol, 45% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 8.95 (s, 1H), 7.91 (s, 1H), 7.87 - 7.81 (m, 2H), 4.96 (dd, =10.5, 2.7 Hz, Hi), 4.81 (d, 7=9.3 Hz, ill).449 (td, 7=104, 48 Hz, 111), 4.20 {hr dd, 7=11.4, 5.0 Hz, 111).4.03 - 3.92 (m, 311), 3.88 (br t, 7=10.1 Hz, 111), 3.78 (brt, 7=6.2 Hz, 1H), 3.72 (brt, 7=11.1 Hz, 1H), 3.55 - 3.48 (m, 3H), 3.24 (s, 3H), 3.21 - 3.15 (m, 111), 2.65 (br s, 1 H), 1.96 (br d, 7=5.8 Hz, 2H), 178 - 1.71 (m, 211).1.67 (br d, 7=12.7 Hz, 1H), 1.36 (brt, 7=9.8 Hz, 4H), 1.28 - 1.18 (m, 211): LC/MS (ESI) mie 641.2

[{M 1!) , calcd for CrsEheFsNeOeS 641.2], fe = 1.99 min (Method 2), 11P1.C (Method 3): /R = 11.24 min; HPLC (Method 4): /R = 9.99 min. hGal-3 ICso = 0.013 mM.

Example 20. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4- (tetrahydro-2i7-pyran-4-yl)- IH- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2i7pyran-3 -yl)thio)-2-

(hydroxymethyl)-5-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- \H~ 1, 2, 3 -triazol- 1- yl)tetrahy dro-2i/-pyran-3 -ol

Example 20 Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4 (tetrahy dro-2/-pyran-4-yl)- \H~ 1 ,2,3-triazol- 1 -yl)tetrahydro-2//-pyran-3-yl)thio)-5- m ethoxy -4-(4~(3 ,4, 5-trifluoropheny!)- 1 //·· 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7-pyran-2- yl)m ethyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2/7~pyran~3 ~yl)thio)~5~methoxy~4-(4-(3 ,4, 5-irifluorophenyl V 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2i7-pyran-2-yi)m ethyl acetate (20 mg, 0.032 mmol) and 4- ethyny!tetrahydro~2/7~pyran (10.72 mg, 0.097 mmol) were dissolved in a previously degassed solution of DMF (0.9 niL) and water (0.300 ml .) and the mixture was placed under argon. Sodium ascorbate (7.71 mg, 0.039 mmol) and copper(II) sulfate pentahydrate (11 34 mg, 0.045 mmol) (predissolved in 0 3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol . Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichorom ethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product w'as purified by column chromatography on silica gel (10% ethyl acetate containing 5% methanol/90% hexanes— > 80% ethyl acetate containing 5% methanol/20% hexanes 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4-(t etrahydro-2i/-pyran-4- yl)~l/: -l ,2,3-triazoi-l-yl)tetrahydro-2/:/~pyran-3-yi)thio)-5-methoxy -4-(4~(3,4,5- trifluorophenyl)- 177-1 ,2,3-triazol-l-yi)tetrahydro-277-pyran-2-yi)methyl acetate (22 mg, 0.030 mmol, 93% yield) as a white solid. ¾ NMR (400 MHz, ACETONITRILE-ds) d 8.32 (s. 1 1 1 ), 7.68 - 7.61 (m, 31 i ), 5.47 - 5 46 (m, 1 H), 5.03 (d l, ,7-10.4, 3. 1 Hz, 1 H), 4.88

(d, J=9.5 Hz, 1 1 1 ). 4.52 (ddd, ./ ! i .0, 9.7, 4.9 Hz, 1H), 4.26 - 4.19 (m, 3H), 4.16 - 4.04 (m, 4H), 4.00 - 3.94 (m, 2H), 3.85 (t, .7-11.2 Hz, 1H), 3.62 - 3 48 (m, 3H), 3.29 (s, 3H), 3.23 (ddd, 7=11.5, 10.2, 4.9 Hz, 1H), 3.00 (tt, 7=11.6, 3.8 Hz, 1H), 2.05 (s, 3H), 1.98 (s, 31 1 ).. 1.92 (dt, 7=13.2, 1 9 Hz, 21 1), 1.71 (qd, .7-12 1, 4 4 Hz, 21 1 ). I .(VMS (ESI) m/e 727.3 [(M+H) + , ealed for C uf 1 M· AALS 727.2], ίk = 1.88 min (Method 2). Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5- (4-(tetrahydro-27/-pyran-4-yl)-l7/-l,2,3-triazol-l-yl)tetrah ydro-2i7-pyran-3-yl)thio)-5- methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 1//-1, 2, 3-tri azol-l-y l)tetrahydro-2i [ /-pyran-2- yl)methyl acetate (22 mg, 0.030 mmol) in THF (0.75 mL) and MeOH (0.75 mL) at it was added sodium methoxide (25% wt solution in methanol) (0.035 mL, 0.151 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and w'as filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy- 5-(4-(tetrahydro-2i7-pyran-4-yl)-l//-l,2,3-triazol-l-yl)tetr ahydro-2i7-pyran-3-yl)thio)-2- (hydroxy methyl)-5-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 1 - 1,2, 3 -triazol- 1- yl)tetrahydro-2i/-pyran-3-ol (11.2 mg, 0.016 mmol, 54% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 8.95 (s, 1 1 1, major), 8.94 (s, H I. minor), 7.99 - 7.97 (m, 1H), 7.88 - 7 81 (m, 2H), 5.14 (dd, ./ 10.5, 2.8 Hz, 1H, minor), 5.01 - 4 93 (m, 1H, major), 4.82 (d, .7=9.3 Hz, 1H), 4.56 - 4.44 (m, 2H), 4.24 - 4.17 (m, 2H), 4.11 - 4.07 (m, 2H, minor), 4.02 (hr dd, .7=40 6, 4 5 Hz, 1 H), 3.99 - 3.86 (m, 4H), 3 80 - 3.71 (m,

4H), 3.53 (dd, 7=6.3, 2.9 Hz, 2H), 3.50 - 3.42 (m, 4H), 3.24 (s, 3H, major), 3.22 (s, 3H, minor), 2.97 - 2.89 (m, 1H), 1 86 (br d, 7=43 6 Hz, 2H), 1 .67 - 1.56 (m, 2H); LC/MS (ESI) m!e 643.2 [(M+H) + , caied for C2.7H34F3N6O7S 643.2], ίk = 1.77 min (Method 2); HPLC (Method 3): fe = 9.14 min; HPLC (Method 4): fe = 7 81 min. hGal-3 ICso = 0.018

Example 21. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4-amino-5- methoxypyrimi din-2-yl)- 177-1 ,2, 3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3- yl)thio)-2-(hydroxymethyl)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2 /-pyran-3-ol

Example 21

Step 1. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4-acetamido-5- methoxypyrimidin-2-yl)-l/ -l,2,3-triazol-l-yl)-4-hydroxytetrahydro-2//-pyran-3- yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5-trifluoroph enyl)-li7-l,2,3-triazol-l- yl)tetrahydro-2 /-pyran-3 -yl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2f/-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2 /-pyran-2-yl)methyl acetate (42 mg, 0 068 mmol) and A ? -(2-ethynyl-5- methoxypyrimidin-4-yl)acetamide (20.84 mg, 0.109 mmol) were dissolved in a previously degassed solution of DMF (1.5 mL) and water (0.500 mL) and the mixture was placed under argon. Sodium ascorbate (16.19 mg, 0.082 mmol) and copper(II) sulfate pentahydrate (23.81 mg, 0.095 mmol) (predissolved in 0.5 ml water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separator} ' funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 20 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% methanol/hexanes—> 100% ethyl acetate containing 5% methanol; 24 g column) to afford (2R,3R,4S,5R,6S)~ 6-(((3R,4R,5S)-5-(4-(4-acetamido-5-methoxypyrimidin-2-yl)-l/ 7-l,2,3-triazol-l-yl)-4- hydroxytetrahydro-2//-pyran-3-yl)thio)-2-(acetoxymethyl)-5-m ethoxy-4-(4-(3,4,5- trifluorophenyl)-l77-l,2,3-triazol-l-yl)tetrahydro-277-pyran -3-yl acetate (25 mg, 0.031 mmol, 45% yield) as a white solid. l H NMR (400 MHz, CHLOROFORM-d) d 8.22 (s, 2H), 8.18 - 8.14 (m, ! H), 7.96 (s, 1 H), 7.56 - 7.44 (m, 2H), 5.52 (d, 7=2.5 Hz, 1 H), 4.85 - 4.76 (m, 2H), 4.55 (td, 7=10.3, 4.5 Hz, 1H), 4.38 - 4.25 (m, 4H), 4.25 - 4.18 (m, 2H),

4.16 - 4.12 (m, 1H), 4.12 - 4.07 (m, 1 1 1), 4.03 (s, 3H), 3.59 (br t, 7=11.8 Hz, 1H), 3.35 (s, 31 1 ), 3.21 (td, 7=10.9, 5.0 Hz, 1H), 2.64 (s, 31 1 ). 2.11 (s, 31 1 ). 1.98 (s, 31 1 ): LC/MS (ESI) m!e 808.3 [(M+H) + , calcd for C33H37F3N9O10S 808.2], /R = 1.85 min (Method 2). Step 2

To a suspension of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4-acetamido-5- methoxypyrimi din-2 -yl)- 1//- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-277-pyran-3 - yl)thio)-2-(acetoxymethyl)-5-methoxy-4-(4-(3,4,5-trifluoroph enyl)-l//-l,2,3-triazol-1 - yl)tetrahydro-277-pyran-3-yl acetate (25 mg, 0.031 mmol) in THF (0.8 mL) and MeOH (0.8 mL) at -20 °C (acetone/dry ice bath) was added sodium methoxide (25% wt solution in methanol) (0.036 mL, 0.158 mmol). The cooling bath was removed and the reaction mixture was stirred at -20 °C for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.03 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-5-(4-(4- amino-5-methoxypyrimidin-2-yl)-1 /7-l ,2,3-triazol-l-yl)-4-hydroxytetrahydro-27/-pyran- 3-yl)thio)-2-(hydroxymethyl)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 1/7-1, 2, 3 -triazol- 1- yl)tetrahydro~2//~pyran~3-ol (15.4 mg, 0.022 mmol, 71% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 8.96 (s, 1 1 1 ). 8.79 (s, 1 1 1). 7.87 - 7.82 (m, 31 1 ), 5.69 (br s, 1 1 1), 5.47 (br s, H I ), 4.97 (dd, .7= 10.5, 2.7 Hz, Hi), 4.84 (d, .7=9.2 Hz, i l l ). 4.73 (id. .7=10.1 , 5.0 Hz, i l l). 4.29 - 4.19 (m, 1H), 4.14 (br dd, .7=10.8, 4.9 Hz, 1 1 1 ). 4.02 - 3.96 (m, 2H), 3.95 (s, 3H), 3.89 (br t, J=l l.l Hz, 2H), 3.79 (br t, ,7=6.3 Hz, 1H), 3.59 - 3.49 (m, 31 1 ), 3.24 (s, 3H), 3.29 - 3.21 (m, 1 H); LC/MS (ESI) m!e 682.3 [(M+H) + , calcd for C2.7H31F3N9O7S 682.2], & = 1.65 min (Method 2); HPLC (Method 3): /R = 7.59 min; HPLC (Method 4): fe = 5.98 min. hGal-3 ICso = 0.023 mM.

Example 22. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(thiazol-

2-yl)-l//-l,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)th io)-2-(hydroxymethyl)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l /-l,2,3-triazol-l-yl)tetrahydro-2i/-pyran-3-ol

Example 22

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4- (thiazol-2-yl)- 177-1,2, 3-triazol-l-yl)tetrahydro-2i/-pyran-3-yl)thio)-5-methoxy-4-( 4- (3 ,4, 5-trifluorophenyl)- 1H~1 ,2,3 -tri azol- 1 ~yl)tetrahydro-2/7-py ran-2-yl)methyl acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2f/-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1 ,2,3 -tri azol- 1 - yl)tetrahydro-2//-pyran-2-yl)methyl acetate (16 mg, 0 026 rnmol) and 2-ethynylthiazole (4.53 mg, 0.042 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (6.17 mg, 0.031 mmol) and copper(IT) sulfate pentahydrate (9.07 mg, 0.036 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in d ichor omethane. The filtrate was transferred to a separatory' funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in di chi or omethane (4 x 15 mL). The combined organic layers were washed with brine (15 mL) The organic layer was dried over MgSCri, filtered, and concentrated. The product was purified by column chromatography on silica gel (20% ethyl acetate containing 5% methanol/hexanes -----> 80% ethyl acetate containing 5% methanol/20% hexanes; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4- hy droxy-5-(4-(thiazol-2-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7-pyran-3 -yl)thio)-5- methoxy-4-(4-(3, 4, 5 -tri fluorophenyl)-! 77-1, 2, 3 -tri azol- l-yl)tetrahydro-277-pyran-2- yl)m ethyl acetate (16 mg, 0.022 mmol, 85% yield) as a white solid. J H NMR (400 MHz, CHLOROFORM-d) d 8.26 (s, 1H), 7.87 (d, 7 3.3 Hz, Hi), 7.84 (s, Hi), 7.47 (dd, .7=8.0, 6.5 Hz, 2H), 7.40 (d, .7=3.3 Hz, 1H), 5.51 (d, 7=3.0 Hz, 1H), 4.74 - 4.64 (m, 2H), 4.60 - 4.52 (m, 1H), 4.45 - 4.23 (m, 4! I), 4.20 - 4.11 (m, 4H), 3.58 - 3 48 (m, H I), 3.35 (s, 31 1),

3.18 (ddd, ,7=1 1.9, 9.7, 5.0 Hz, ! H), 2.11 (s, 3H), 1.94 (s, 3H); LC/MS (ESI) m/e 726.2 [ { \ 1 H ) _ calcd for C29H31F3N7O8S2 726.2], fe = 1.34 min (Method 2).

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-

(4-(thi azol-2-yl)- 1 H- 1 ,2,3 -triazol - 1 -yl)tetrahydro-2//-pyran-3 -yl)thio)-5-methoxy-4-(4- (3 ,4, 5-trifluorophenyl)- 177-1 ,2,3 -tri azol- 1 -yl)tetrahydro-277-py ran-2-yl)methyl acetate (16 mg, 0.022 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at rt (acetone/dry ice bath) was added sodium methoxide (25% wt solution in methanol) (0.025 mL, 0.110 mmol). The cooling bath was removed and the reaction mixture was stirred at rt. for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(thiazol-2 -yl)-177-l ,2,3-triazol-l- yl)tetrahydro-277-pyran-3-yl)thio)-2-(hydroxymethyl)-5-metho xy-4-(4-(3,4,5- trifluorophenyl)-177- l ,2,3-triazol-l-yl)tetrahydro-277-pyran-3-ol (13.3 mg, 0.020 mmol, 92% yield) as a white amorphous solid. *H NMR (500 MHz, DMSO-de) d 8.96 (s, 1H), 8.79 (s, i l l ), 7.94 (d, .7=3.4 Hz, 1H), 7 85 (dd, .7=8.9, 6.7 Hz, 21 1), 7.78 (d, .>3 2 Hz, 1H), 4.97 (dd, >10.5, 3.1 Hz, 1H), 4.84 (d, >9.3 Hz, 1H), 4.70 - 4.61 (m, 1H), 4.23 (br dd, >1 1.8, 4.8 Hz, H i ).. 4.12 (dd, >10.8, 5.0 Hz, H I ).. 4.03 - 3.93 (m, 41 1 ), 3.84 (br t, >11.1 Hz, 1H), 3.80 (br t, >6.4 Hz, 1H), 3.58 - 3.49 (m, 4H), 3.25 (s, 3H), 3.29 - 3.22

(m, H I ): LC/MS (ESI) mle 642.2 [(M+H) + , calcd for C25H27F3N7O6S2 642 1], fe = 1.82 min (Method 2); HPLC (Method 3): t& = 9.76 min; HPLC (Method 4): /R = 8.54 min. hGal-3 ICso = 0.005 mM Example 23. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2-

(trifluoromethyl)pyrimidin-5-yl)-I7/-l,2,3-triazol-l-yl)t etrahydro-2//-pyran-3-yl)thio)-2- (hydroxymethyl)-5-methoxy-4-(4-(3 ,4, 5-tri fluorophenyl)-! ff-1 , 2, 3-triazol-l- yl)tetrahy dro-277-pyran-3 -ol

Example 23

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2 - (trifluoromethyl)pyrimidin-5-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-27/-pyran-3 -yl)thio)-5- methoxy-4-(4-(3, 4, 5-tri fluorophenyl)- 1 /7-1, 2, 3-triazo!-l~yl)tetrahydro-2i -pyran-2~ yl)m ethyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro-

2//-pyran-3 -yl)thio)-5-methoxy-4-(4-(3 ,4, 5-trifluorophenyl)- 1 H- 1,2,3 -triazol- 1 yl)tetrahydro-2//-pyran-2-yl)methyl acetate (20 mg, 0.032 mmol) and 5-ethynyl-2- (trifluoromethyl)pyrimidine (8.93 mg, 0.052 mmol) were dissolved in a previously degassed solution of DMF (0.9 niL) and water (0.300 niL) and the mixture was placed under argon. Sodium ascorbate (7.71 mg, 0.039 mmol) and eopper(II) sulfate pentahydrate (11.34 mg, 0.045 mmol) (predissolved in 0.5 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (10 mL) and saturated aqueous sodium bicarbonate (10 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 10 mL). The combined organic layers were washed with brine (20 mL). The organic layer was dried over MgSCri, filtered, and concentrated. The product was purified by column

chromatography on silica gel (25% ethyl acetate containing 5% methanol/75% hexanes 100% ethyl acetate containing 5% methanol; 12 g column) to afford

((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4 -(2- (trifluoromethyl)pyrimidin-5-yl)-l//-l ,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3-yl)†hio)-5- m ethoxy -4-(4~(3 ,4, 5-trif uorophenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-2- yl)m ethyl acetate (12 mg, 0 015 mmol, 47% yield) as a white solid. (H NMR (400 MHz,

CHLOROFORMS) d 9.36 (s, 2H), 8.22 (s, 1H), 7.84 (s, 1H), 7.47 (dd, 7=8.0, 6.5 Hz, 21 1 ), 5.53 (d, ./ 3 0 Hz, 1 1 1 ), 4.74 - 4.65 (m, 21 1 ). 4 58 (td, .>=10 3, 4 8 Hz, i l l), 4.43 (dd, 7 1 1 .4, 4.9 Hz, 1H), 4.33 (br dd, .7=11.9, 5.1 Hz, 1 1 1). 4.27 (t, 7=10.0 Hz, 2H), 4.16 - 4.13 (m, 3H), 3.54 (t, .7=12.0 Hz, 2H), 3.36 (s, 3H), 3.15 (ddd, J=\ 1.9, 9.8, 5.1 Hz, Hi), 2.11 (s, 3H), 1.97 (s, 3H); LC/MS (ESI) m!e 789.3 [(M+H) + , calcd for CsiHsiFeNeOsS

789.2], fe = 2.13 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5- (4-(2-(trifluoromethy 1 )pyrimidin-5-yl)- 177- 1 ,2,3 -triazol- 1 -yi)tetrahydro-2/7-pyran-3 - yl)thio)-5~methoxy~4-(4-(3, 4, 5-tri fluorophenyl)-!//-! , 2, 3-triazol-i-yl)tetrahydro-2//~ pyran-2-yl)methyi acetate (12 mg, 0.015 mmol) in THF (0.5 mL) and MeOH (0.5 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.017 mL, 0.076 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.18 mL). The mixture was concentrated and was taken up in dioxane/rnethanol/water and was filtered and purified by reverse phase preparative HPLC (Method 3). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford

(2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(2-(triflu oromethyl)pyrimidin-5-yl)- 177-l,2,3-triazol-I-yl)tetrahydro-2/7-pyran-3-yl)thio)-2-(hy droxymethyl)-5-methoxy-4- (4-(3,4,5-trifluorophenyl)-l 7/-1 ,2,3-triazol-l-yl )tetrahydro-2/7-pyran-3-ol (7.3 mg, 9.84 mhioΐ, 65% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO-de) d 9.49 (s, 2H), 9.08 (s. 1 1 1 ), 8.99 (s. 1 1 1 ). 7 85 (hr dd, .7=8.8, 6 8 Hz, 2H), 6.53 (s, 1H), 5.76 (d, .7=7.0 Hz, 1H), 5.49 (d, .7=6.3 Hz, 1H), 4.97 (dd, J=10.5, 2.7 Hz, 1H), 4.85 - 4.79 (m, 2H), 4.73 - 4.65 (m, H I ). 4.27 - 4.22 (m, H i), 4.17 (br dd, .7=11.1 , 4.9 Hz, 1 1 1), 4.02 - 3.97 (m, 1H), 3.96 - 3.92 (m, 2H), 3.89 - 3.83 (m, IH), 3.83 - 3.77 (m, 1H), 3.55 - 3.51 (m, 3H), 3.25 (s, 3H); LC/MS (ESI) m/e 705.2 [(M+H) + , calcd for C^&TFeNsOeS

705.2], hi = 1 .92 min (Method 2); HPLC (Method 3): t« = 10.58 min; HPLC (Method 4): /R = 9.38 min. hGal-3 ICso = 0.041 mM.

Example 24. Preparation of methyl 6-(l -((3S,4R,5R)-4-hydroxy-5-(((2S,3R,4S,5R,6R)- 5-hydroxy -6-(hydroxymethyl)-3-methoxy-4-(4-(3, 4, 5-trifluorophenyl)- 177-1, 2, 3-triazol- l -yl)tetrahydro-27/-pyran-2-yl)thio)tetrahydro-277-pyran-3-yl )- 177-1 , 2, 3-triazol-4- yl)picolinate

Step 1 Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(6- cyanopyri din-2-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)-4-hydroxytetrahydro-27/~pyran-3 -yl)thio)-5- methoxy-4~(4-(3,4,5~tritluorophenyl)~l/: -l,2,3-triazol-l-yl)tetrahydro-2//~pyran-2- yl)methyl acetate

(+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2/ -pyran-3-yl)thi o)-5-methoxy-4-(4-(3, 4, 5-tri fluorophenyl)-! 1,2, 3-tri azol-1- yl)tetrahydro-2/ -pyran-2~yl)methyl acetate (20 mg, 0.032 mmol) and 6- ethynylpicolinonitrile (12.47 nig, 0.097 mmol) were dissolved in a previously degassed solution of DMF (0.9 mL) and water (0.300 mL) and the mixture was placed under argon. Sodium ascorbate (7.71 mg, 0.039 mmol) and copper(II) sulfate pentahydrate (11.34 mg, 0.045 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (5 mL) and saturated aqueous sodium bicarbonate (5 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Brine (10 mL) was added and the aqueous layer was extracted with 5% methanol in dichloromethane (3 x 10 mL). The combined organic layers were washed with brine (10 mL). The organic layer was dried over MgSOr, filtered, and concentrated. The product was purified by column chromatography on silica gel (40% ethyl acetate containing 5% methanol/60% hexanes 100% ethyl acetate containing 5% methanol; 12 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4- (6-cyanopyiidin-2-yl)-f//~l,2,3-triazol-l~yl)-4-hydroxytetra hydro-2/:/-pyran-3~yl)thio)~ 5-methoxy-4-(4-(3,4,5-trifluorophenyl)-li/-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-2- yl)methyl acetate (22 mg, 0.030 mmol, 91% yield) Ή NMR (400 MHz,

CHLOROFORMS) d 8.41 - 8.39 (m, 1H), 8.42 (br d, 7=1.0 Hz, 1H), 7.94 (t, 7=7.9 Hz, 1H), 7.87 (s, 1H), 7.65 (dd, J= 7.8, 1.0 Hz, 1H), 7.47 (dd, 7=8.0, 6.5 Hz, 2H), 5.51 (d,

./ 2.5 Hz, 1H), 4.74 - 4 67 (m, 2H), 4.61 - 4.52 (m, III), 4 40 (dd, ./ 1 1 .5, 4.8 Hz, 1H), 4.34 - 4.22 (m, 3H), 4.19 - 4.03 (m, 5H), 3.51 (t, .7=1 1.9 Hz, IH), 3.35 (s, 3H), 2.10 (s,

3 ! I ) 1.96 (s, 3H); LC/MS (ESI) m/e 745.2 [(M+H) + , calcd for (Ή I vRXxOxS 745 2], /¾ = 1.86 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-5-(4-(6- cyanopyridin-2-yl)-l /7-l ,2,3-triazol-l-yl)-4-hydroxytetrahydro-2 /-pyran-3-yl)thio)-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-l/ -l,2,3-triazol-l-yl)tetrahydro-2i -pyran-2- yl)methyl acetate (22 mg, 0.030 mmol) in THF (0.5 rnL) and MeOH (0.5 mL) at rt was added sodium methoxide (25% wt solution in methanol) (0.034 mL, 0.148 mmol) and the mixture was stirred for 1 h. The mixture was concentrated and was taken up in dioxane/m ethanol/water and was filtered and purified by reverse phase HPLC (Method 4) The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford methyl 6-(l-((3S,4R,5R)-4-hydroxy-5-(((2S,3R,4S,5R,6R)-5- hydroxy-6-(hydroxymethyl)~3m:iethoxy~4--(4-(3, 4, 5~tri fluorophenyl)-- li/~l, 2, 3-triazol-l - y 1 Hetrahy dro-2 /-pyran-2-y 1 )thio)tetrahydro-2i7-pyran-3 -yl)- 1 H- 1 , 2,3 -triazol -4- yl)picolinate (4.5 mg, 0.0054 mmol, 18% yield) as a white amorphous solid. ¾ NMR (400 MHz, DMSO-de) d 8.98 (s, 1 H), 8.82 (s, 1 H), 8.28 (dd, 7=7.9, 0.9 Hz, 1 H), 8 12 (t, 7=7.8 Hz, 1H), 8.02 (dd, 7=7.7, 0.9 Hz, 1H), 7.86 (dd, 7=8.8, 7.0 Hz, 2H), 6.57 (br s,

H i ).. 5.69 (d, 7=7.0 Hz, 1H), 5 49 (d, 7=6 3 Hz, H i), 4.98 (dd, 7=10 7, 2 6 Hz, H I ), 4.84 (d, 7=9.3 Hz, l i l ). 4.79 (br s, 1H), 4.69 (td, 7=10.4, 5.0 Hz, 1H), 4.27 - 4.20 (m, IH),

4.1 1 (br dd, 7=10 7, 4 9 Hz, 1 1 1), 4.05 (br d, 7=7.3 Hz, IH), 4 01 - 3.94 (m, 2H), 3.93 (s, 3H), 3.91 - 3.86 (m, 1H), 3.80 (t, 7=6.3 Hz, IH), 3.54 (br d, 7=1 1.3 Hz, 3H), 3.26 (s, 31 1 ); LC/MS (ESI) m/e 694.2 [(M+H) , calcd for 04 I nl-VN OKS 694.2], fe = 1.76 min (Method 2), S iPl .C (Method 3): to = 14.01 min, HPLC (Method 4): h <. = 12.10 min. hGal-3 ICso = 0.014 mM.

Example 25. Preparation of (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4-(pyrazin- 2-yl)- 1 H- 1 ,2,3-triazol- 1 -yl)tetrahydro-2//-pyran-3-yl)thio)-2-(hydroxymethyl)-5- methoxy -4-(4-(3 ,4, 5 -trifl uorophenyl)- 1 H -1,2,3 -triazoi - 1 -y Ijtetrahy dro-2// -pyran-3 -ol

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-(4- (pyrazin-2-yJ)-li/-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3 -yl)thio)-5-methoxy-4-(4- (3,4,5 -trifluoropheny 1 )- !//- 1 ,2, 3 -tri azol- 1 ~y!)tetrahy dro-2/:/ -py ran-2-yl)methy 1 acetate (+)-((2R,3R,4S,5R,6S)-3-Acetoxy-6-(((3R,4R,5S)-5-azido-4-hyd roxytetrahydro- 2//-pyran-3-yl)thio)-5-methoxy-4-(4-(3,4,5-trifluorophenyl)- li/-l,2,3-triazol-l- yl)tetrahydro-2 /-pyran-2-yl)methyl acetate (62 mg, 0.101 rnmol) and 2-ethynylpyrazine (16.75 mg, 0.161 mmol) were dissolved in a previously degassed solution of DMF (3 mL) and water (1.000 ml) and the mixture was placed under argon. Sodium ascorbate (23.90 mg, 0.121 mmol) and copper(II) sulfate pentahydrate (35.2 mg, 0.141 mmol) (predissolved in 0.5 rnL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (10 mL) and saturated aqueous sodium bicarbonate (10 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel . Brine (1(3 mL) was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 15 mL). The combined organic layers were washed with brine (15 mL). The organic layer was dried over MgS0 4 , filtered, and concentrated. The product was purified by column chromatography on silica gel (30% ethyl acetate containing 5% methanol/ hexanes 100% ethyl acetate containing 5%

methanol/hexanes; 24 g column) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-

4-hydroxy-5-(4-(pyrazin-2-yl)-l /7-l ,2,3-triazol-l-yl)tetrahydro-2i7-pyran-3-yl)thio)-5- methoxy-4-(4~(3,4,5-trifluorophenyl)-li/-L2,3~triazoi-l-yl)t etrahydro-2/f-pyran~2- yl)m ethyl acetate (65 mg, (3 09(3 mmol, 90% yield) as a white solid. 41 NMR (4(30 MHz, CHLOROFORMS) d 9.44 (d, 7=0.8 Hz, 1H), 8.55 (s, 2H), 8.35 (s, 1H), 7.83 (s, 1H), 7.47 (dd, .7=8.0, 6 5 Hz, 2H), 5.51 (d, 7=2.8 Hz, H I ), 4.74 - 4.64 (m, 2H), 4 62 - 4 52 (m, 1H), 4.40 (dd, 7=11.5, 5.0 Hz, 1H), 4.32 (dd, 7=11.8, 5.0 Hz, 1 1 1). 4.26 (t, 7=9.9 Hz, 1H), 4.20 - 4.09 (m, 4H), 4 09 - 4 03 (m, 1 H), 3.52 (t, 7=1 1.9 Hz, 1H), 3 36 (s, 3H), 3 17 (ddd.

7=1 1 .8, 9.8, 5.0 Hz, i l l ), 2.1(3 (s, 31 1 ), 1.94 (s, 3H); 1.C7MS (ESI) mie 721 .2 [(M+H) + , calcd for CsoHxrFsNsOgS 721.2], /R = 1.88 min (Method 2).

Step 2

To a suspension of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-4-hydroxy-5-

(4-(pyrazin-2-yl)-l//-l,2,3-triazol-l-yl)tetrahydro-2i7-p yran-3-yl)thio)-5-methoxy-4-(4- (3,4,5 -trifluoropheny 1 )- !//- 1,2, 3 -tri azol- 1 -yl)tetrahy dro-2/7 -py ran-2~yl)methy 1 acetate (65 mg, 0.090 mmol) in THF (2 mL) and MeOH (2 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.103 mL, 0.451 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture %vas partially neutralized by the addition of 1 N HC1 (0.2 mL). The mixture was concentrated and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase HPLC (Method 4). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford (2R,3R,4S,5R,6S)-6-(((3R,4R,5S)-4-hydroxy-5-(4- (pyrazin-2-yJ)-li/-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3 -yl)thio)-2-(hydroxymethyJ)-

5-methoxy-4-(4-(3,4,5-trifluorophenyl)-li/-l,2,3-triazol- l-yl)tetrahydro-2//-pyran-3-ol (46 mg, 0.072 mmol, 80% yield) as a white amorphous solid. f H NMR (500 MHz, DMSO-de) d 9.26 (d, >1.1 Hz, 1H), 8.96 (s, 1H), 8.87 (s, 1H), 8.73 - 8.67 (m, 1H), 8.63 (d, .>2 4 Hz, ! l ! ). 7.90 - 7.80 (m, 2H), 5.74 - 5.61 (m, 1H), 5.46 (br d, >1 .2 Hz, 1 H), 4.98 (dd, >10.6, 2.8 Hz, 1H), 4.84 (d, >9.3 Hz, 1H), 4.68 (td, >10.4, 4.9 Hz, 1H), 4.24 (br dd, >1 1.4, 4.7 Hz, 1H), 4 13 (dd, >10.9, 5.0 Hz, 1H), 4 04 - 3.93 (m, 3H), 3.86 (t, >11.1 Hz, 1H), 3.80 (t, >6.3 Hz, 1H), 3.59 - 3.51 (m, 3H), 3.25 (s, 3H), 3.29 - 3.22 (m,

1 1 1 ): LC/MS (ESI) m/e 637.2 [(M+H) + , calcd for C^eHisF NsOeS 637 2], fe = 1 77 min (Method 2); HPLC (Method 3): t& = 8.84 min; HPLC (Method 4): ίk = 7.54 min. hGai-3 ICso = 0.016 mM.

Example 26. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-

5-(4-(pyrimidin-5-yl)-l//-l,2,3-triazol-l-yl)tetrahydro-2 i/-pyran-3-yl)thio)-6-

(hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-I /7-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran-

3-yl)oxy)acetic acid

Step 1 Preparation of methyl 2-(((2S,3R4S,5R6R)-5-acetoxy-6-(acetoxymethyl)-2- (((3R,4R, 5 S)-4-hydroxy-5-(4-(pyrimidin-5-yl)- 1 H- 1 ,2, 3 -triazol- 1 -yl)tetrahydro-2> pyran-3-yl)thio)-4-(4-(3,4,5-trifluorophenyl)-li -l,2,3-triazol-l -yl)tetrahydro-2//-pyran-

3-yl)oxy)acetate Methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2-(((3R,4R, 5S)-5- azido-4-hydroxy tetrahydro-2i7-py ran-3 -yl)thio)-4-(4-(3 ,4, 5-tri fluorophenyl)- 1 H- 1 ,2,3- triazol-l-yl)tetrahydro-2/7-pyran-3-yl)oxy)acetate (101 mg, 0.150 mmol) and 5- ethynylpyrimidine (31.2 mg, 0.299 mmol) were dissolved in a previously degassed solution of DMF (2.7 mL) and water (0.9 mL) and the mixture was placed under argon. Sodium ascorbate (29.7 mg, 0.150 mmol) and copper(II) sulfate pentahydrate (29.9 mg, 0.120 mmol) (predissolved in 0.3 mL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (10 mL) and saturated aqueous NaHCCb solution (10 mL) were added resulting in the formation of a precipitate. The mixture was filtered through a pad of Celite and the filter cake was rinsed with 5% methanol in dichoromethane. The filtrate was transferred to a separatory funnel. Several milliliters of brine was added and the aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 15 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine was rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgSOy filtered, and concentrated. The product was purified by column chromatography on silica gel (50% ethyl acetate containing 5% methanol/50% hexanes— > 100% ethyl acetate containing 5% methanol; 24 g column) to afford methyl 2~(((2S,3R,4S,5R,6R)-5- acetoxy-6-(acetoxymethyl)-2-(((3 R,4K, 5 S)-4-hy droxy-5 -(4-(pyrimi din-5 -yl)- 177- 1 ,2, 3 - triazol~l-yl)tetrahydro-2/:/-pyran-3~yl)thio)~4-(4-(3,4,5~ti iiluorophenyl)-l//-l,2,3-triaz.ol- l-yl)tetrahydro-2/7-pyran-3-yl)oxy)acetate (81.2 mg, 0.104 mmol, 70% yield) as a white solid. ¾ NMR (400 MHz, CHLOROFORM-d) d 9.23 (s, 1H), 9.21 (s, 2H), 8.13 (s, 1H), 7.87 (s. 1 1 1 ), 7.45 (dd, .7=7.8, 6.8 Hz, 21 1 ). 5 55 (d, 7-3.0 Hz, i l l), 4 85 - 4 75 (ns. 2H),

4.61 - 4.49 (m, 2H), 4.44 - 4.28 (m, 4H), 4.21 (td, 7=9.8, 2.3 Hz, 1H), 4.16 (s, 3H), 4.14 - 4.02 (m, 21 1 ), 3.62 (s, 31 1 ), 3.53 (t, 7=11.9 Hz, 1H), 3.19 - 3 08 (m. 1 1 1 ), 2.98 (s. 1 1 1 ),

2.91 (s, 1H), 2.08 (s, 3H), 2.01 (s, 31 1 ): LC/MS (ESI) m/e 779.2 [(M+H) + , caicd for C32H34F3N8O10S 779.3], /; 1.91 min (Method 1). Step 2

To a suspension of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (((3R,4R, 5 S)-4-hydroxy-5-(4-(pyrimidin-5-yl)- 1 H- 1 ,2, 3 -triazol- 1 -yl)tetrahydro-2i/- pyran-3 -y 1 )thio)~4-(4-(3 ,4, 5 -trifluoropheny 1 )- YH~ 1 ,2, 3 -tri azol- 1 -yl)tetrahy dro-2/7-py ran- 3-yl)oxy)acetate (80 mg, 0.103 mmol) in THF (2 mL) and MeOH (2 niL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.1 17 mL, 0.514 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (0.1 mL). The mixture was concentrated and was taken up in dioxane/m ethanol /water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2-(((2S,3R,4S,5R,6R)- 5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-(pyrimidin-5-yl)-lf& gt;l,2,3-triazol-l- yl)tetrahydro-2/7-pyran-3-yl)thio)-6-(hydroxymethyl)-4-(4-(3 ,4,5-trifluorophenyl)-l /- l,2,3-triazol-l-yl)tetrahydro-2i/-pyran-3-yl)oxy)acetic acid (66.5 mg, 0.098 mmol, 95% yield) as a white amorphous solid. *H NMR (500 MHz, DMSO-de) d 9.24 (s, 2H), 9.18 (s, 1H), 8.91 (d, .>3 8 Hz, 2H), 7.80 (dd, >8.8, 6.8 Hz, 2H), 5.88 - 5.66 (m, 1H), 5 52 - 5.37 (m, 1H), 5.05 (dd, >10.5, 2.8 Hz, 1H), 4.86 (d, >9.3 Hz, 1H), 4.65 (td, >10.3, 5.0 Hz, 1H), 4 47 (d, >16.2 Hz, 1H), 4 30 - 4.19 (m, 2H), 4.15 (or dd, >10.8, 5.0 Hz, ! ! ! ), 4.00 - 3.88 (m, 3H), 3.86 - 3.78 (m, 2H), 3.54 - 3.45 (m, 2H), 3.26 (td, >10.8, 4.9 Hz, 1 H); LC/MS (ESI) m!e 681 .2 [(M+H) + , calcd for OLsiriNsOsS 681 1 ], /¾ = 1 58 min

(Method 1); HPLC (Method 1): t& = 5.07 min; HPLC (Method 2): ίk = 4.74 min. hGal-3 ICso = 0.015 mM.

Example 27. Preparation of methyl 2-(((2S,3R,4S,5R,0R)-5-hydroxy-2-(((3R,4R,5S)-4- hydroxy-5-(4-(pyrimidin-5-yl)-l /-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-I /7-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran- 3-yl)oxy)acetate

Example 27

To a solution of 2-(((2S,3R,4S,5R,0R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(pyrimidin-5-yl)-l /7-I ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yl)thio)-6- (hy droxymethyl)-4-(4-(3 ,4,5 -trifluoropheny 1)- IH- 1,2, 3 -triazol- 1 -yl)tetrahy dro-2//-pyran- 3-yl)oxy)acetic acid (16.5 nig, 0.024 mmol) in CH2CI2 (0.9 niL) and MeOH (0.3 mL) at 0 °C was added trimethylsilyidiazomethane (0.077 mL, 0.048 mmol). The reaction mixture was stirred at rt for 2.5 h. The mixture was concentrated. The product was tritrated with ether and was dried under vacuum to afford methyl 2-(((2S,3R,4S,5R,6R)- 5~hydroxy~2~(((3R,4R,5S)~4~hydroxy~5~(4-(pyrimidin-5-yl)-Ii7 ~I,2,3-triazol~l~ yl)tetrahydro-2i/-pyran-3-yl)thio)-6-(hydroxymethyl)-4-(4-(3 ,4,5-trifluorophenyl)-li/- 1,2, 3 -triazol- l-yl)tetrahydro-2//-pyran-3-yl)oxy)acetate (12.5 mg, 0.016 mmol, 65% yield) as a white solid 1H NMR (500 MHz, DMSO-de) d 9.24 (s, 2H), 9.18 (s, I H), 8.91 (d, 7=3.8 Hz, 2H), 7.80 (dd, 7=8.6, 6.9 Hz, 2H), 5.81 (d, ,7=6.9 Hz, IH), 5.48 (d, 7=6.3 Hz, IH), 5 07 (dd, 7=10.5, 2.6 Hz, IH), 4 85 (d, 7=9.3 Hz, IH), 4.81 (t, 7=5.5 Hz, I H), 4.69 - 4.61 (m, IH), 4.58 (d, 7=16.0 Hz, IH), 4.28 - 4.21 (m, 2H), 4.15 (br dd, 7=11.0,

5.0 Hz, IH), 4 07 (d, 7=16.0 Hz, IH), 3.95 (br dd, 7=5.7, 2.4 Hz, IH), 3.93 - 3.87 (m,

IH), 3.85 - 3.80 (m, 2H), 3.57 - 3.46 (m, 3H), 3.39 (s, 3H), 3.29 - 3.23 (m, IH); LC/MS (ESI) m!e 695.2 [(M+H) + , calcd for C2&H30F3N8O8S 695.2], /R = 1.69 min (Method 1);

I IPi .C (Method I): /¾ = 5.86 min; HPLC (Method 2): fe = 5 54 min hGal-3 ICso = 0.017 u.M

Example 28. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy- 5-(4-(pyrimidin-5-yl)-l//-l,2,3-tiiazol-l-yl)tetrahydro-2i7- pyran-3-yl)thio)-6-

(hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l7/-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-

3-yl)oxy)-l-morpholinoethan-l-one

Example 28

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(pyrimidin-5-yl)- 1 H- 1 ,2,3 -triazol- 1 -yDtetrahy dro-2//-pyran-3 -yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triaz ol-l-yl)tetrahydro-2//-pyran- 3-yl)oxy)acetic acid (17 mg, 0.025 mmol) and morpholine (4.31 mΐ, 0.050 mmol) in

DMF (0 8 niL) was added A^A-diisopropylethylamine (0.022 mL, 0. 125 mmol) followed by HATU (18.99 mg, 0.050 mmol). The reaction mixture was stirred at it for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2- (((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-( pyriimdin-5-yl)-l//- l,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yl)thio)-6-(hydrox ymethyl)-4-(4-(3,4,5- trifluorophenyl)- ! H- \ ,2,3 -triazol- 1 ~yl)tetrahydro-2iT-pyran-3 -yl)oxy)- 1 - morpholinoethan-l-one (15.1 mg, 0.020 mmol, 80% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 9.25 (s, 2H), 9.18 (s, 1 1 1 ).. 8.93 - 8.88 (m, 2H), 7.84 - 7.74 (m, 2H), 5.25 (hr dd, 7=10.4, 2.7 Hz, 1 ! !, minor), 5.11 - 5.03 (m, 2H), 4.89 (d, 7=9.3 Hz, 1H), 4.68 - 4.60 (m, 1H), 4.56 (d, ,7=12.4 Hz, 1H), 4.52 (dd, 7=7.7, 4.8 Hz, 1H), 4.28 - 4 19 (m, 2H), 4.18 - 4.12 (m, i l l), 4 09 (br d, 7=10 7 Hz, I I I. minor), 4.05 - 4.00 (m, 1 1 1), 3.99 - 3.92 (m, 2H), 3.89 - 3.80 (m, 2H), 3.57 - 3.49 (m, 3H), 3.41 - 2.97 (m, 10H);

I .C/MS (ESI) mie 750 2 [(M+H) + , calcd for CsiftsFsNgOeS 750.2], IR = 1.57 min

(Method 1); HPLC (Method G): ίk = 5.18 min; HPLC (Method 2): /s = 4.93 min. hGal-3 ICso = 0 010 mM.

Example 29. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy- 5-(4-(pyrimidin-5-yl)-l//-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triaz ol-l-yl)tetrahydro-2i -pyran· 3 -y 1 )oxy)-A -methyl-A-phenylacetami de

Example 29

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(pyrimidin-5-yl)-l/ -!,2,3~triazol-l-yl)tetrahydro~2/: -pyran~3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-tiifluorophenyl)-li7-l,2,3-triaz ol-l-yJ)tetrahydro-2i/-pyran- 3-yl)oxy)acetic acid (16 mg, 0.024 mmol) and /V-methylaniline (5.09 mΐ, 0.047 mmol) in DMF (0 8 tnL) was added AyV-diisopropylethylamine (0.021 mL, 0. 1 18 mmol) followed by HATU (17.88 mg, 0.047 mmol). The reaction mixture was stirred at rt for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2~

(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-( 4-(pyrimidin-5-yl)-li/- 1 ,2,3 -triazo!- 1 -yl)tetrahy dro~2/:/~pyran~3 -yl)thio)-6-(hy droxymethy l)-4-(4-(3 ,4, 5- trifluoropheny 1 )- 1 H- 1,2,3 -triazol- 1 -yl)tetrahydro-2i/-pyran-3 -yl)oxy)-N-methyl -N- phenylacetamide (4.6 mg, 5.92 pmol, 25% yield) as a white amorphous solid. ‘H NMR (500 MHz, DMSO-de) d 9.26 (s, 2H), 9.19 (s, 1 H), 8.95 - 8.89 (m, 1H), 8.81 (br s, 1 H), 7.78 - 7.68 (m, 2H), 7.30 - 7.16 (m, 3H), 7.12 - 7.00 (m, 2H), 5.82 - 5.76 (m, 1H, minor), 5.70 (br s, 1 H, major), 5.39 (br d, ,7=4.0 Hz, 1 H, major), 5 23 - 5.14 (m, 1 H, minor), 5.04 - 4.95 (m, 1H), 4.84 (br d, 7=8.9 Hz, 1H, major), 4.80 - 4.71 (m, 1H, minor), 4.68 - 4.57 (m, 1 1 1 ), 4.49 (br s, H I, minor), 4.25 - 3.76 (m, 9H), 3 49 (br t, ./ 1 1 .6 Hz, 31 1 ), 3.22 - 3.13 (m, 1H), 2.95 (s, 3H, major), 2.93 (br s, 3H, minor); LC/MS (ESI) m!e 770.2

[ (M I I ) , ca!cd tor C34H35F3N9O7S 770.2], fe = 1.80 min (Method 1), HPLC (Method 1): hi = 6.43 min; HPLC (Method 2): t& = 6.10 min. hGal-3 IC50 = 0.025 mM. Example 30. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy- 5-(4-(2-methoxypyrimidin-5-yl)-li7-l ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-6-

(hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)- 1 H- 1 ,2,3-triazol- 1 -yl)tetrahydro-2i/-pyran-

3~yl)oxy)acetic acid

Example 30

Step 1. Preparation of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (((3R,4R,5S)-4-hydroxy-5-(4-(2-methoxypyrimidin-5-yl)-li/-I ,2,3-triazol-l- y l)tetrahydro-2Z/-pyran-3 -y l)thio)-4-(4-(3 ,4,5-trifluoropheny 1)- 1 H- 1,2,3 -triazol- 1 - yl)tetrahydro-2 /-pyran-3-yl)oxy)acetate

Methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2-(((3R,4R, 5S)-5- azido-4-hy droxytetrahydro-2/:/-py ran-3 -yl)thio)-4-(4-(3 ,4, 5-tri fluorophenyl)- 1//- 1 ,2,3 - triazol-l-yl)tetrahydro-2//-pyran-3-yl)oxy)acetate (250 mg, 0.371 mmol) and 5-ethynyl- 2-methoxypyrimidine (99 mg, 0.741 mmol) were dissolved in a previously degassed solution of DMF (6 ntL) and water (2 ml.) and the mixture w'as placed under argon.

Sodium ascorbate (73.4 mg, 0.371 mmol) and copper(II) sulfate pentahydrate (74.0 mg, 0.296 mmol) (predissolved in 0.3 ntL water) were added and the reaction mixture was stirred at room temperature for 14 h. The mixture and the solid clinging to the stir bar were dissolved by the addition of dichloromethane and methanol. Water (15 mL) and saturated aqueous sodium bicarbonate (15 mL) were added resulting in the formation of a precipitate. The aqueous layer was extracted (with gentle shaking) with 5% methanol in dichloromethane (4 x 25 mL). The combined organic layers were washed with brine (10 mL). The separatory funnel containing the brine w ? as rinsed with dichloromethane (2x) to collect the yellow residue and this organic layer was combined with the original organic extract. The organic layer was dried over MgS0 4 , filtered, and concentrated.

The product was purified by column chromatography on silica gel (50% ethyl acetate containing 5% methanol/50% hexanes 100% ethyl acetate containing 5% methanol; 40 g column) to afford methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2-

(((3R,4R,5S)-4-hydroxy-5-(4-(2-methoxypyrimidin-5-yl)-li/ -I,2,3-triazol-l- y 1 )tetrahydro-27/~pyran-3 -y l)thio)-4-(4-(3 ,4,5-trifluoropheny 1)- 1 H- 1,2,3 -triazol- 1 - yl)tetrahydro-2 /-pyran-3-yl)oxy)acetate (260 mg, 0.321 mmol, 87% yield) as a white solid. Ή NMR (400 MHz, DMSO-de) d 9.04 (s, 2H), 8.89 (s, 1H), 8.76 (s, 1H), 7.78 (dd, J= 8 8, 6 8 Hz, 2H), 5.83 (d, 7=6.8 Hz, H I), 5.40 (d, 7=2.8 Hz, 11 1).. 5.36 (dd, 7=10.4,

2.9 Hz, i l l), 5 05 (d, 7=9 5 Hz, 1H), 4.64 (id, 7=10.4, 4.9 Hz, 1 H), 4.54 (d, 7=16.1 Hz, 1H), 4.40 - 4.31 (m. 2H), 4.24 - 4.14 (m, 2H), 4.11 - 4.01 (m, 3H), 3.98 (s, 3H), 3.96 - 3.81 (m, 2H), 3.53 (br t, 7=1 1.5 Hz, I I I ), 3.38 (s, 3 i I ), 3.25 (id. 7=10.8, 4.8 Hz, 1H), 2 03 (s, 3H), 2.02 (s, 3H); LC/MS (ESI) m/e 809.3 [(M+H) + , calcd for CssHseFsNsOiiS 809.2], in : z 1.89 min (Method 1).

Step 2

To a suspension of methyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (((3R,4R,5S)-4-hydroxy-5-(4-(2~methoxypyrimidin-5-yl)-l/7-!, 2,3~tiiazol-l- yl)tetrahydro-2//-pyran-3-yl)thio)-4-(4-(3,4,5-trifluorophen yl)-l//-l,2,3-triazol-l - yl)tetrahydro-2// pyran-3~yl)oxy)acetate (217 mg, 0.268 mmol) in THF (3 mL) and MeOH (3 mL) at 0 °C was added sodium methoxide (25% wt solution in methanol) (0.307 mL, 1.342 mmol). The cooling bath was removed and the reaction mixture was stirred at rt for 1 h. The mixture was partially neutralized by the addition of 1 N HC1 (1 mL). The mixture was partially concentrated to remove the THF and was taken up in dioxane/methanol/water and was filtered and purified by reverse phase preparative MPLC using a 08 Redisep Gold column (2 injections) (Method 1). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2- (((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-( 2-methoxypyrimidin-5- yl)-l//-l,2,3-triazol-l-yl)tetrahydro-2i/-pyran-3-yl)thio)-6 -(hydroxymethyl)-4-(4-(3,4,5- trill uorophenyl)-!/:/- 1 ,2, 3-triazol-l-yl)tetrahydro-2 /-pyran-3-yl)oxy)acetic acid (162.5 mg, 0.226 mmol, 84% yield) as a white amorphous solid. Ή NMR (500 MHz, DMSO- de) d 12.36 (br s, 1 1 1), 9.05 - 9.00 (m, 2H), 8 89 (s, i l l ). 8 75 (s, i l l ). 7 85 - 7.72 (m, 2H), 5.71 (br d, 7=6.6 Hz, 1H), 5 42 (d, J=6.1 Hz, 1H), 5.05 (dd, .7=10 4, 2.4 Hz, 1 1 1). 4.85 (d, ,7=9.3 Hz, 1H), 4.77 (br t, ,7=5.2 Hz, 1H), 4.62 (td, 7=10.3, 5.0 Hz, 1H), 4.46 (d, 7=15.9 Hz, 1 ! ! ). 4 28 - 4.18 (m, 2H), 4.13 (br dd, ,7=10.8, 4.9 Hz, 1 H), 3.97 (s, 3H), 3.96 - 3.88 (m, 3H), 3.85 - 3.78 (m, 2H), 3.56 - 3.46 (m, 3H), 3.29 - 3.21 (m, I l f ); LC/MS (ESI) m/e

71 1 .2 [(M+H) + , calcd for Cbrf-feoIUNsCLS 711 2], /¾ = 1 60 min (Method 1); HPLC

(Method 1): fe = 5.78 min; HPLC (Method 2): fe = 5.45 min. hGal-3 ICso = 0.008 mM.

Example 31. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy- 5-(4-(2-methoxypyrimidin-5-yl)-lf7-4,2,3-triazoi-l-yl)tetrah ydro-27/-pyran-3-yl)thio)-6-

(hydroxymethydV4-(4-(3,4,5-trifluorophenyd)-!//~i,2,3-tri azol-l~yl)tetrahydro-2//-pyran-

3-yl)oxy)-l -morpholinoethan-1 -one

Example 31

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(2-methoxypyrimidin-5-yl)-l7/-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyJ)-17/-l,2,3-triaz ol-l-yl)tetrahydro-2i7-pyran· 3-yl)oxy)acetic acid (24 mg, 0 034 mmol) and morpholine (5 83 mΐ, 0 068 mmol) in DMF (0.8 mL) was added Ay¥-diisopropylethylamine (0 029 mL, 0.169 mmol) followed by HATU (25.7 mg, 0.068 mmol). The reaction mixture was stirred at rt for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2- (((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-( 2-rnethoxypyrirnidin-5- yl)-l/7-l ,2,3-triazol-l-yJ)tetrahydro-2/7-pyran-3-yl)thio)-6-(hydroxy methyl)-4-(4-(3,4,5- trifluorophenyl)- ! H- \ ,2,3 -triazol- 1 ~yl)tetrahydro-2i7-pyran-3 -yl)oxy)- 1 - morpholinoethan-l-one (25.1 mg, 0.031 mmol, 93% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 9.03 (s, 2H), 8.90 (s, 1H, major), 8.88 (s, IH, minor), 8.75 (s, 1H), 7.84 - 7.77 (m, 2H), 5.25 (dd, ,/ 10.2, 2,7 Hz, 1 H, minor), 5.09 - 5.02 (m, 1H), 4.88 (d, ,7=9.2 Hz, 1H), 4.62 (td, .7=10.3, 5.0 Hz, 1H), 4.55 (d, ,7=12.4 Hz, 1H), 4.53

- 4 48 (m, I H), 4.27 - 4.20 (m, 3H), 4 18 - 4 10 (m, 2H), 4.04 - 3.99 (m, 1H), 3 97 (s, 3H), 3.96 - 3.91 (m, IH), 3.83 (td, ,7=1 1.1, 7.2 Hz, 2H), 3.55 - 3.48 (m, 3H), 3.41 - 2.98 (m, 10H); LC/MS (ESI) mie 780.3 } { \ 1 1 1 ) . calcd for C32H37F3N9O9S 780.3], fe = 1.68 min (Method 1); HPLC (Method 1): in = 5.94 min; HPLC (Method 2): t& = 5.66 min. h Gal-3 ICso = 0.013 mM.

Example 32. Preparation of l-(2,6-dimethylmorpholino)-2-(((2S,3R,4S,5R,6R)-5- hy droxy-2-(((3R,4R, 5 S)-4-hy droxy-5 -(4-(2-methoxypyrimidin-5-yl)- 1 H- 1 ,2,3 -triazol- 1 - yl)tetrahydro-2/7-pyran-3-yl)thio)-6-(hydroxyniethyl)-4-(4-( 3,4,5-trifluorophenyl)-17/- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2i/-pyran-3 -yl)oxy)ethan- 1 -one

Example 32

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(2-methoxypyrimidin-5-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2i/-pyran-3 -yl)thi o)-6-

(hydroxymethyl)-4~(4-(3,4,5~trifluorophenyl)~l/7-l,2,3-tr iazol-I-yl)tetrahydro~27/~pyran- 3-yl)oxy)acetic acid (23 mg, 0.032 mmol) and 2,6-dimethylmorpholine (7.46 mg, 0.065 mmol) in DMF (0.8 mL) was added AyA-diisopropylethylamine (0.028 mL, 0.162 mmol) followed by HATU (24.61 mg, 0.065 mmol). The reaction mixture was stirred at rt for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2). TWO peaks were present which had the correct mass. The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford l-(2,6- dimethylmorpholino)-2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4 R,5S)-4-hydroxy-5-(4- (2-methoxypyrimidin-5-yl)-17/-l ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l7/-l,2,3-triaz ol-l-yl)tetrahydro-27/-pyran-

3-yl)oxy)ethan-l -one (4.1 mg, 4 91 pmol, 15% yield) (Isomer 1) as a white amorphous solid and l-(2,6-dimethyimorphofino)-2-(((2S,3R,4S,5R,6R)-5-hydroxy-2- (((3R,4R,5S)-

4-hydroxy-5-(4-(2-methoxypyrimi din-5-yl)- 1/7- 1 ,2,3 -tri azol- 1 ~yl)tetrahydro~2/7~pyran~ 3-yl)thio)-6-(hydroxymethyl)-4-(4-(3, 4, 5-tri fluorophenyl)- 177-1,2, 3-tri azol- 1- yl)tetrahydro-2 /-pyran-3-yl)oxy)ethan-l-one (21.5 mg, 0.026 mmol, 80% yield) (Isomer 2) as a white amorphous solid. The major peak (Isomer 2) was isolated and

characterized. 1 H NMR (500 MHz, DMSO-dcf) 6 9.02 (s, 2H), 8.93 - 8.86 (m, 1H), 8.75 (s. 1 1 1 ), 7.85 - 7.74 (m, 2H), 5.25 (br d, ./ 10 ! Hz, i l l, minor), 5.06 (hr d, .7=10.7 Hz, 1H), 4.87 (br t, .7=9.4 Hz, 1H), 4.65 - 4.48 (m, 3H), 4 28 - 3.99 (m, 5H), 3.97 (s, 3H), 3.96 - 3.88 (m, 3H), 3 86 - 3 79 (m, 2H), 3.55 - 3.48 (m, 3H), 3 41 - 3.15 (m, 5H), 2.44 -

2.32 (m, 1H), 2.09 - 1 94 (m, 1H), 0.96 - 0.89 (m, 6H); LC/MS (ESI) mie 808.3

I ( \ 1 · 1 1) . calcd for C34H41F3N9O9S 808.3], fe = 1.84 min (Method 1); HPLC (Method 1): 2k = 6.59 min; HPLC (Method 2): <k = 6.21 min. hGal-3 IC50 = 0.019 mM Example 33. Preparation of l-(l,l-dioxidothiomorpholino)-2-(((2S,3R,4S,5R,6R)-5- hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-(2-methoxypyrimidin-5- yl)-I77-l,2,3-triazol-l - yl)tetrahydro-2i7-pyran-3-yl)thio)-6-(hydroxymethyl)-4-(4-(3 ,4,5-trifluorophenyl)-l7/-

1 ,2,3 -tri azol- 1 -yl)tetrahydro-27/-pyran-3 -yl)oxy)ethan- 1 -one

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(2-methoxypyrimidin-5-yl)- 1 /'/· 1 ,2,3 -triazol- 1 -yi)tetrahydro-2i7-pyran-3 -yl)thi o)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triaz ol-l-yl)tetrahydro-2//-pyran- 3-y])oxy)acetic acid (23 mg, 0 032 rnmol) and ihiomorphoiine 1 ,1 -dioxide (8 75 mg, 0 065 mmol) in DMF (0.8 mL) was added AW-diisopropylethylamine (0.028 mL, 0.162 mmol) followed by HATU (24.61 mg, 0.065 mmol). The reaction mixture was stirred at rt for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford l-(l, l -dioxidothiomorpholino)-2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-((( 3R,4R,5S)- 4-hydroxy-5-(4-(2-methoxypyrimidin-5-yl)-li/-l,2,3-triazol-l -yl)tetrahydro-2//-pyran- 3-yl)†.hio)-6-(hydroxymethyl)~4-(4-(3, 4, 5~tri fluorophenyl)- 17/-T, 2, 3-†.riazol-l - yl)tetrahydro-2//-pyran-3-yl)oxy)ethan-l-one (25.5 rng, 0.030 mmol, 92% yield) as a white amorphous solid. ¾ NMR (500 MHz, DMSO-de) d 9.03 (s, 2H), 8.91 (s, IH, major), 8.89 (s, 1 H, minor), 8.75 (s, 1H, major), 8.74 (s, 1 H, minor), 7.85 - 7.76 (m, 2H), 5.29 - 5.24 (m, 1H, minor), 5.08 (dd, ,7=10.5, 2.7 Hz, 1H), 5.04 (d, J= 9.5 Hz, 1H, minor), 4.87 (d, .7=9.2 Hz, 1H), 4 67 - 4.58 (m, 21 1 ), 4.57 - 4.52 (m, i l l minor), 4.29 - 4.05 (m, 6H), 3.97 (s, 3H), 3.96 (hr d, 7=3. 1 Hz, 1H), 3.94 - 3.90 (m, 1H), 3.86 - 3.79 (m, 2H), 3.73 - 3 59 (m, 31 1 ), 3.56 - 3.45 (m, 5H), 3 28 (id, 7=10 8, 5.0 Hz, 1 1 1), 3.12 - 2.86 (m,

4H); LC/MS (ESI) m/e 828.2 [(M+H) + , caicd for C32H37F3N9O10S2 828.2], & = 1.57 min (Method 1); HPLC (Method 1): ts. = 5.94 min; HPLC (Method 2): fa = 5.71 min. hGal-3 ICso = 0 012 mM. Example 34. Preparation of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-

5-(4-(2-methoxypyrimidin-5-yl)-177-1 ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-6-

(hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)- 1 H- 1 ,2,3-triazol- 1 -yl)tetrahydro-277-pyran- 3 -yl)oxy)- 1 -(3 -hydroxyazetidin- 1 -yl)ethan- 1 -one

Example 34

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(2-methoxypyrimidin-5-yl)-l7/-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-I /7-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran- 3-yl)oxy)acetic acid (23 mg, 0.032 mmol) and azeti din-3 -ol HCl (7.09 mg, 0.065 mmol) in DMF (0 8 niL) was added iV, V-diisopropyl ethyl amine (0.028 mL, 0. 162 mmol) followed by HATU (24.61 mg, 0.065 mmol). The reaction mixture was stirred at rt for 14 h. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2). The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4 -(2- methoxypyrimidin-5-yl)- \H- 1 ,2,3-triazol- 1 -yDtetrahy dro-2//-pyran-3 -yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-I /7-l,2,3-triazol-l -yl)tetrahydro-2/7-pyran- 3-yl)oxy)-l-(3-hydroxyazetidin-l-yl)ethan-l-one (18.7 mg, 0.024 mmol, 73% yield) as a white amorphous solid. ‘H NMR (500 MHz, DMSO-de) d 9.03 (s, 2H), 8.95 - 8.90 (m, 1H), 8.75 (s, 1H), 7.84 - 7.78 (m, 2H), 5.23 (br d, ,7=10.4 Hz, 1H, minor), 5.05 (hr dd,

./ i 0.5. 1.9 Hz, Hi), 4.86 (br dd, ./ 9 3, 2.3 Hz, 1 1 1 ), 4.62 (td, ./ 10.3 , 5.0 Hz, i l l ). 4 40 (br d, ,7=13.6 Hz, 1H), 4.36 - 4.08 (m, 5H), 3.97 (s, 3H), 3.96 - 3.84 (m, 4H), 3.84 - 3.78 (m, 3H), 3.69 (br dd, .7=9.7, 4.5 Hz, i l l, minor), 3 57 - 3 48 (m, 31 1 ), 3.41 (br dd, ./ 10.2, 3.8 Hz, 1H), 3.31 - 3.21 (m, 1 1 1); LC/MS (ESI) mie 766.2 [( M i l) calcd for C31H35F3N9Q9S 750.2], tR = 1.54 min (Method 1); HPLC (Method 1): fe = 5.19 min; HPLC (Method 2): /¾ = 4 87 min. hGai-3 IC50 = 0 024 mM.

Example 35. Preparation of l-(azetidin-l-yl)-2-(((2S,3R,4S,5R,6R)-5-hydroxy-2- (((3R,4R,5S)-4-hydroxy-5-(4-(2~methoxypyrimidin-5-yl)-li -i,2,3~triazoi-l- yl)tetrahydro-2i7-pyran-3-yl)thio)-6-(hydroxymethyl)-4-(4-(3 ,4,5-tri fluorophenyl)- IH- l,2,3-triaz.ol-i-yl)tetrahydro-2//~pyran-3-yl)oxy)ethan-l~on e

Example 35

To a solution of 2-(((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5- (4-(2-methoxypyrimidin~5-yi)-17/~l,2,3-triazol-l -yl)tetrahydro-2//~pyran-3-yl)thio)-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-l//-l,2,3-triaz ol-l-yl)tetrahydro-2i -pyran- 3-yl)oxy)acetic acid (14 mg, 0 020 mmol) and azetidine (9.00 mg, 0.158 mmol) in DMF (0.6 mL) was added AyV-diisopropylethylamine (0.017 mL, 0.099 mmol) followed by HATU (14 98 mg, 0.039 mmol). The reaction mixture was stirred at it for 4 h. LC/MS showed that only ca. 30% conversion to product occurred. The reaction mixture was heated to 50 °C and additional azetidine (9.00 mg, 0.158 mmol) and HATU (14.98 mg, 0.039 mmol) was added and stirring was continued for 1 h. Additional azetidine (9.00 mg, 0.158 mmol) and HATU (14.98 mg, 0.039 mmol) was added two more times at 45 min intervals. Most of the starting material was converted to product. The mixture was diluted with acetonitrile and a small amount of water and was filtered and purified by reverse phase preparative HPLC (Method 2) The organic solvent was evaporated on the rotovapor and the aqueous mixture was lyophilized to afford l-(azetidin-l-yl)-2- (((2S,3R,4S,5R,6R)-5-hydroxy-2-(((3R,4R,5S)-4-hydroxy-5-(4-( 2-methoxypyrimi din-5- yl)-li7-l,2,3-triazol-l-yl)tetrahydro-2i/-pyran-3-yl)thio)-6 -(hydroxymethyl)-4-(4-(3,4,5- trifluorophenyl)- \H~\ ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-3 -yl)oxy)ethan- 1 -one (8.6 mg, 0.01 1 mmol, 57% yield) as a white amorphous solid. Ή NMR (500 MHz,

METHANOL-d-s) 6 9.01 (s, 2H), 8.67 (s, III), 8.56 (s, H i ), 7.68 (dd, ./ 8 6, 6 6 Hz, 2H), 5.02 (dd, ,7=10.5, 3.0 Hz, 1H), 4.82 (s, 1H, partially obscured), 4.73 - 4.66 (m, 1H), 4.46 (d, .7=14.0 Hz, Hi), 4.38 - 4.30 (m, 2H), 4.24 (dd, 7=11.1, 4 9 Hz, H i), 4. 15 - 4 09 (m, 3H), 4.08 (s, 3H), 4.04 - 3.77 (m, 8H), 3.75 - 3.70 (m, 1H), 3.61 (t, .7=1 1.7 Hz, 1H), 2.19 (quin, 7=7.8 Hz, 2H); LC/MS (ESI) m/e 750 3 [ (M I I ) , calcd for C31H35F3N9O8S 750.2], tR = 1.82 min (Method 1); HPLC (Method 1): 2k = 5.87 min; HPLC (Method 2):

5.63 min. hGal-3 IC50 = 0.032 mM

Preparation of Intermediates (2)

Preparation of 4-ethynylpyridazine

To a 200 mL pear shaped flask was added pyridazine-4-carbaldehyde (0.10 g, 0.93 mmol), K2CO3 (0.26 g, 1.9 mmol), and MeOH (9 mL). The reaction was stirred for 10 min, then dimethyl (l-diazo-2-oxopropyl)phosphonate (0.19 g, 0.97 mmol) was added. After stirring 18 h, the mixture was diluted with ether (50 mL) and washed successively with 1.5 M K2.HPO4 (aq.) (2 x 25 mL) and brine. The organic phase was dried over Na2S0 4 , filtered and concentrated. The residue was purified by silica gel (12 g cartridge; A = Hex, B = EtOAc; 15 min grad.; 0% B to 70 %B; flow rate = 12 mL/min). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (17 mg, 0.16 mmol, 18% yield) as a tan solid. ! H NMR (400 MHz, CHLOROFORM-d) d 9 16 - 9.10 (m, 2H), 7.47 - 7.40 (m, 1 1 1 ).. 3.42 (s, 1 1 1 )

Preparation of ((2i?,3i?,45',5i?,6i?)-3-acetoxy-4-azido-6-bromo-5-methoxyte trahydro-2/7- py ran -2-y 1 )m ethyl acetate

To a 250 mL round bottomed flask was added ((2/i3f?,4S',5i?,65)~3-acetoxy-4~ azido-5-methoxy-6-(phenylthio)tetrahydro-2//-pyran-2-yl):met hyl acetate (1.5 g, 3.7 mmol) and DCM (60 mL). The vessel was cooled to 0 °C, then bromine (0.28 mL, 5.5 mmol) dissolved in DCM (15 mL) was added dropwise. The reaction was stirred at the above temperature for 1 h. Excess bromine was quenched with a mixture of 1 N K2HPO4 (aq.) and sat NaiSCL (aq.) (1 : 1, 20 mL). The mixture was further diluted with water (100 mL) and the layers were separated. The aqueous phase was extracted with DCM (3x50 mL). The organic phase was combined, washed with brine, dried over NauSCE, filtered and concentrated. The product was dried in vacuo to provide the title compound (0.87 g, 2.4 mmol, 65% yield) as a white solid which was either used immediately or stored in the freezer ¾ NMR (400 MHz, CHLOROFORM-d) 5 6.72 - 6.64 (m, 1 ! ! }.

5.48 - 5.40 (m, 1H), 4.49 - 4.35 (m, H i). 4.24 - 4.14 (m, 1 1 1), 4.11 - 4.04 (m, 1 1 1). 4.01 - 3.91 (m, 1 1 1 ).. 3.59 - 3.56 (m, 1 1 1 ), 3.55 (s, 31 1 ), 2.22 - 2.14 (m, 311), 2.13 - 2.04 (m, 3H). LC/MS (ESI) m/e 387.5 [(M+H 2 0) + , calcd for CuHieBrNsOe 366.2], t K = 1.64 min (Method 2).

Preparation of ((2i?,3i?,45',5i?,6.S)-3-acetoxy-4-azido-6-(((3i?,4i?,55)-4- hydroxy-5-(4- ph eny 1 -1 -1 ,2,3 -tri azol - 1 -y 1 )tetrahy dro-2//- py ran -3 -y 1 )th i o)- 5 -m eth oxy tetrahy dro-2 H- pyran-2-yl)methyl acetate

Step 1. Preparation of (3»S , ,4/?,5S)-3-hydroxy-5-(4-phenyl-l//-l,2,3-triazol-I- yl)tetrahydro~2/:/-pyran~4-yl acetate To a 100 mL pear shaped flask was added (3A,4i?,5A)-3-azido-5- hydroxytetrahydro~2iT-pyran~4-yi acetate (0.52 g, 2.6 mmol), ethynylhenzene (0.85 mL, 7.8 mmol), followed by a degassed mixture of DMF (12 mL) and water (4 mL). To this mixture was added copper(II) sulfate pentahydrate (0.45 g, 1.8 mmol) and sodium ascorbate (0.51 g, 2.6 mmol) dissolved in water (2 mL). The vessel was evacuated and flushed with N2. After stirring 18 h, the reaction was filtered and the filter cake was washed with DCM. The filtrate was diluted with 1 N K2HPO4 (aq.) (200 mL), and the aqueous phase was extracted with DCM (3x100 mL). The organic phase was combined, washed with brine, dried over Na^SCfi, filtered and concentrated. The crude product was purified by flash column chromatography (40 g silica gel cartridge; A = Hex, B =

EtOAc; 20 min grad ; 0% B to 100% B, flow rate = 40 mL/rnin). The pure fractions w ? ere combined, concentrated and dried in vacuo to provide the title compound (0.38 g, 1.3 mmol, 49% yield) as a white solid j o- in 22 +36.8, (c ------ 1.0, CHCI3), 'l l NMR (500

MHz, METHANOL-d- 6 8.49 - 8 45 (m, I I I), 7.88 - 7.79 (m, 2H), 7 50 - 7 42 (m, 21 1), 7.42 - 7.33 (m, 1H), 5.57 - 5.45 (m, 1H), 5.19 - 5.07 (m, 1H), 4.26 - 4.22 (m, 11 1). 4.22 -

4.19 (m, 11 1).. 4.10 - 4.02 (m, I I I), 4.01 - 3 95 (m, 11 1), 3.84 - 3.77 (m, i l l), 2 02 - 1.91 (m, 3H); LC/MS (ESI) m/e 304.1 [(M+H) + , calcd for C15H17N3O4 303.3], is = 0.71 min (Method 4). Step 2. Preparation of (3i?,4i?,55)-3-(acetylthio)-5-(4-phenyl-I/7-l,2,3-triazol-l- yl)tetrahydro-2/7-pyran-4-yl acetate

To a 100 mL pear shaped flask w-as added (3A,4i?,55)-3-hydroxy-5-(4 phenyl-l//- 1,2,3 -triazol- 1 -yl)tetrahy dro-2J7-pyran-4-yl acetate (0.37 g, 1.2 mmol), DCM (24 mL), and pyridine (0.40 mL, 4.9 mmol). The reaction w¾s cooled to ---10 °C, then

trifluoromethanesulfonic anhydride (0.41 mL, 2.4 mmol) was added over a period of 5 min. The reaction was stirred at the above temperature for 1 h. The mixture was diluted with DCM (25 mL) and washed successively with 1 M HCi (aq.) (3 x 25 mL), 1 M K2HPO4 (aq.) (25 mL) and brine. The organic layer was dried over NaiSOr, filtered and concentrated. The resultant residue was dissolved in acetone (20 mL) and cooled to -10 °C. To this mixture was added potassium thioacetate (0.56 g, 4.9 mmol) and the reaction was warmed to it and stirred. After 18 h, the mixture was diluted with water (TOO mL) and extracted with DCM (3 x 50 mL). The organic phase was combined, washed with brine, dried over NaiSOr, filtered and concentrated. The residue was purified by flash column chromatography (80 g silica gel cartridge; A = : Hex, B = EtOAc; 30 min grad.; 0% B to 60% B; flow rate = 60 mL/min). The pure fractions were combined,

concentrated and dried in vacuo to provide the title compound (0.35 g, 0.97 mmol, 80% yield) as a white solid. [CC]D 22 +139.7, (c 1.0, CHCh); l H NMR (500 MHz,

CHLOROFORM-d) d 7.86 - 7.79 (m, 3H), 7.45 (s, 2H), 7.40 - 7.34 (m, 1H), 5.64 - 5.52 (m, 111), 4.85 (d, ./ 47 Hz, 111), 4.34 (br d, .7=5.0 Hz, ill).423 - 4.11 (m, 111), 3.99 - 3.92 (m, 111), 3.92 - 3.84 (m, 1H), 3.62 - 3.54 (m, 111), 2.39 (s, 311), 1.94 (s, 311);

LC/MS (ESI) m/e 3622 [(M II) , calcd for C17H1 9 N3O4S 361.4], t R = 2885 min

(Method 3).

Step 3. Preparation of (3i?,4/?,55)-3-mercapto-5-(4-phenyl-I/7-l,2,3-triazol-l- yl)tetrahydro-2i/-pyran-4-ol

To a 100 mL pear shaped flask was added (3/(,4/(,5/S)-3-(acetylthio)-5-(4~phenyl~ 177-l,2,3-triazol-l-yl)tetrahydro-2i7-pyran-4-yl acetate (0.35 g, 0.97 mmol), and THF (13 mL). The reaction was cooled to -10 °C, then sodium methoxide (0.22 mL, 0.97 mmol) (25% w/v solution in MeOH) was added and the reaction was stirred at the above temperature. After 20 min, the solvent was concentrated and the residue was purified by flash column chromatography (12 g silica gel cartridge, A = Hex, B ::: EtOAc; 15 min grad.; 0% B to 100% B; flow' rate = 12 mL/min). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (0.14 g, 0.52 mmol, 54% yield) as a white solid [of 22 -13.7, (c = 1.0, MeOH); ¾ NMR (500 MHz,

ACETONITRILE-da) d 8.20 - 8.17 (m, 1H), 7.91 - 7.86 (m, 2H), 7.52 - 7.46 (m, 2H), 7.42 - 736 (m, HI), 4.57 - 4.48 (m, ill).424 - 416 (m, 111), 4.13 - 4.07 (m, ill).391 (t, .7=11.1 Hz, 2H), 3.88 - 3.84 (m, Hi).3.45 (t, .7=11.6 Hz, 1H), 3.04 - 2.95 (m, 111).1.91 - 1.79 (m, 111). LC/MS (ESI) m/e 2781 l(M P) , eaicd for C13H15N3O2S 277.3], t R 0.75 min (Method 4).

Step 4

To a 20 mL pear shaped flask was added (37?,47?,55)-3-mercapto-5-(4-phenyl-17/- l,2,3-triazol-l-yl)tetrahydro-27/-pyran-4-ol (0.14 g, 0.52 mmol), ((2R3RAS,5R,6R)-3- acetoxy-4-azido-6-bromo-5-methoxytetrahydro-277-pyran-2-yl)m ethyl acetate (0.20 g, 0.55 mmol), tetrabutylammonium hydrogen sulfate (0.71 g, 2.1 mmol), and EtOAc (7 niL) To this mixture was added 2 M sodium carbonate (aq.) (1 .1 mL, 2. 1 mmol) and the reaction was vigorously stirred. After 2 h, the mixture was diluted with 1 M K2HPO4 (50 mL) and extracted with EtOAc (3 x 25 ml). The organic phase was combined, washed with brine, dried over Na2S0 4 , filtered and concentrated. The residue was purified by flash column chromatography (12 g silica gel cartridge, A = Hex, B = EtOAc; 15 min grad.; 0% B to 100%B; flow 7 rate = 12 mL/min). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (0.240 g, 0.427 mmol,

82% yield) as a white solid [of 22 +1.2, (c = 1 .0, MeOH); ' l l NMR (500 MHz, ACETONITRILE-ds) d 8 21 (s, 1H), 7.90 - 7 85 (m, 2H), 7.52 - 7.45 (m, 2H), 7 42 - 7 35 (m, 1H), 5.38 - 5.32 (m, 1H), 4.71 (d, J=9.6 Hz, 1H), 4.63 - 4.53 (m, 1H), 4.24 - 4.16 (m, 2H), 4.1 1 - 3.96 (m, 3H), 3.90 (s, 1 H), 3.82 - 3.75 (m, 1H), 3.61 (s, 3H), 3.58 - 3 52 (m, 1H), 3.42 - 3.36 (m, 1H), 3.34 - 3.28 (m, 1H), 3.24 - 3.14 (m, 1H), 2.16 (s, 3H), 2.14 - 2.12 (m, 3H), LC/MS (ESI) m/e 563 3 l(M P ) , ea!cd for CwHioNeOeS 562.6], t R 3.68 min (Method 3).

Preparation of (2R,3i?,45',5i?,65)-6-(((3i?,4i?,5 S)-4-acetoxy-5-(4-phenyl-l//-l,2,3-triazol- l-yl)tetrahydro-2/ -pyran-3-yl)thio)-2-(acetoxymethyl)-4-amino-5-methoxytetrahy dro- 2//-pyran-3-yl acetate

Step 1. Preparation of (2^,3i?, S,5i?,65')-6-(((3i?,4i?,5,S ' )-4-acetoxy-5-(4-phenyl-l//-l,2,3- triazol-l -yl)tetrahydro-2/ -pyran-3-yl)thio)-2-(acetoxymethyl)-4-azido-5- methoxytetrahy dro-2i/-pyran-3 -yl acetate

To a 20 mL pear shaped flask were added ((2/i,3K, 45)57?, 60 -3-acetoxy-4-azido- 6-( (3i?,4i?, 56)-4-hydroxy-5-(4-phenyl -IH- 1,2,3 -tri azol- 1 -yl)tetrahydro-2i7-pyran-3 - yi)thio)-5-methoxytetrahydro-2/:/-pyran~2-yi)methyl acetate (70 mg, 0 12 mmol), DCM (3 mL), and pyridine (20 pL, 0.25 mmol). To this mixture was added acetyl chloride (11 pL, 0.15 mmol) and the reaction was stirred under N2. After 18 h, additional pyridine (25 pL, 0.31 mmol) and acetyl chloride (22 pL, 0.31 mmol) were added and the reaction was continued. After 18 h, the solvent was concentrated and the crude product was purified by flash column chromatography (12 g silica gel cartridge; A = Hex, B =

EtOAc; 10 min grad.; 0% B to 100% B; flow rate = 12 mL/min). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (50 mg, 0.083 mmol, 67 % yield) as a white solid. l H NMK (400 MHz, CHLOROFORM-d) d 7.79 ~ 7.71 (m, 3H), 7 39 - 7 32 (m, 2H), 7.30 - 7.24 (m, 1H), 5 46 - 5.36 (m, 1H), 5.33 -

5.26 (m, 1H), 4.80 - 4.67 (m, ! H), 4.46 - 4.40 (m, III), 4.28 - 4 18 (m, 2H), 4.06 - 3.98 (m, 2H), 3.87 - 3.76 (m, 2H), 3.64 - 3.56 (m, 1H), 3.52 (s, 3H), 3.51 - 3.47 (m, IH), 3.24 - 3.05 (m, 2H), 2.09 (s, 3H), 2.02 (s, 3H), 1.90 (s, 3H). LC/MS (ESI) m/e 605.2

[(M+H) + , calcd for C26H32N0O9S 604.6], tR = 0.94 min (Method 4).

Step 2

To a 10 mL round bottomed flask were added (2R,3RAS,5R,6S)-6~(((3R,4R,5S)- 4-acetoxy-5-(4-phenyl-li/-l,2,3-triazol-l-yl)tetrahydro-2//- pyran-3-yl)thio)-2- (acetoxymethyl)-4-azido-5-methoxytetrahydro-2//-pyran-3-yl acetate (50 mg, 0.083 mmol), PI13P (43 mg, 0.17 mmol), water (30 pL, 1.7 mmol), and THF (3 mL). The reaction was stirred at reflux. After 18 h, the solvent was concentrated and the residue was purified by flash column chromatography (12 g silica gel cartridge, A = Hex, B = EtOAc; 15 min grad.; 0% B to 100%B; flow rate = 12 mL/min). The pure fractions were combined, concentrated and dried in vacuo to afford the title compound (63 mg, 0.058 mmol, 70 % yield) as a white solid. Ή NMR (400 MHz, CHLOROFORM-d) d 7.74 - 7.72 (m, 1 1 1 ).. 7.64 - 7.56 (m, IH), 7.51 - 7 44 (m, IH), 7.42 - 7.32 (m, 2H), 7 30 - 7 24 (m, IH), 5.43 (s, IH), 4.94 (d, .7=3.7 Hz, 1 1 1). 4.79 - 4.67 (m, IH), 4.47 (dd, 7=9.9, 1.8 Hz, 1H), 4.30 - 4.19 (m, 3H), 4.18 - 4.08 (m, 2H), 4.03 (dt, J=4.8, 2.2 Hz, 1H), 3.81 (t, ,/ 1 1 .2 Hz, 1 H), 3.62 (t, ./ 1 1.9 Hz, i l l), 3.40 (s, 3H), 3.33 (s, H I), 3.16 - 3 06 (m, 1 H), 2.06 (s, 3H), 1.93 (d, J=I A Hz, 3H), 1.89 (s, 3H). LC/MS (ESI) m/e 579.2 [(M+H) + , ea!cd for C26H34N4O9S 578 6], te ::: 0.70 min (Method 4).

Example Al . Preparation of (2/\ J ,3i?,4ri,5A,65’)-6~(((3i<’,4//,5ri’)-4~hydrox y~5~(4-phenyl- 177-1,2,3 -tri azol- 1 -yl)tetrahy dro-277-pyran-3 -yl)thi o)-2-(hy dr oxymethyl )-5 -m ethoxy-4-

(4-(pyri din- 3 -yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/7-pyran-3 -ol

THF

Example A1

To a 20 mL pear shaped flask was added ((2 ?,3/^,46’,5i¾,65)-3-acetoxy-4-azido-6- (((37?,47?,55)-4-hydroxy-5-(4-phenyl-177-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3- yl)thio)-5-methoxytetrahydro-277-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 3- ethynyl pyridi e (8.3 mg, 0 080 mmol ) followed by DMF (1 L) and water (0.3 mL).

The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6 3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na2S0 4 , filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and TTIF (3 mL).

To this mixture was added sodium meth oxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (11 mg, 0.020 mmol, 71% yield). l B NMR (500 MHz, DMSO-de) d 9 10 (s, 1H), 8.86 (s, 1H), 8.60 (s, 1 1 1 ). 8.57 - 8.53 (m, 1H), 8.28 - 8.19 (m, 1H), 7.84 (hr d, .7=7.6 Hz, 21 1), 7.55 - 7.41 (m, 3H), 7 40 - 7.31 (m, 1 1 1 ), 5.00 - 4.93 (m, 1 1 1 ).. 4.86 - 4.79 (m, 1H), 4.65 - 4.55 (m, 1H), 4.28 - 4.21 (m, 1H), 4.16 - 4.10 (m, 1H), 4.09 - 4.03 (m, 1 1 1). 4.00 (br s, 2H), 3.82 (br d, J= 3.7 Hz, 2H), 3.63 - 3.50 (m, 3H), 3.42 - 3.29 (m, 1H), 3.25 (s, 3H); Analytical Li VMS (ESI) m/e 582.1 | (M 1 1 ) . calcd for C.H M X-CLS 581.7], fe = 1.32 min (Method 1); Analytical LC./MS (ESI) m/e 582.1 [(M+H) + , calcd for

C H L iX-CLS 581.7], fe == 1 17 min (Method 2) hGal-3 ICso = 1 . 1 mM.

Example A2. Preparation of (2f?,3i?,4A5f?,65)-6-i((3f?,4f?,5^-4-hydroxy-5-i4-phenyl- l/:/-l ,2,3~triazoi~l-yl)tetrahydro~2/:/-pyran~3-yl)thio)~2-(hydrox ymethyl)-5~methoxy~4~

(4-(pyri din-4-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2//-pyran-3 -ol

Example A2

To a 20 mL pear shaped flask was added ((2f?,3f?,45 , ,5i?,6A -3 acetoxy-4-azido-6- (((3/\ J ,4i?,50)-4-hydroxy-5-(4-phenyl-li/~l,2,3-triazol-l -yl)teiTahydro-2//-pyran-3- yl)thio)-5-methoxytetrahydro-2i/-pyran-2-yi)methyi acetate (15 mg, 0.027 mmol) and 4- ethynylpyridine (8.3 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL).

The vessel w=as evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Xa.’SO i, filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (13 mg, 0.022 mmol, 82% yield) ' l l \\1R (500 MHz, DMSO-de) d 9.07 - 9.03 (m, 1 H), 8.68 - 8.62 (m, 3H), 7.90 - 7 86 (m, 2H), 7.86 - 7.81 (m, 2H), 7 50 - 7 44 (m, 2H), 7.37 - 7.32 (m, 1H), 5.73 - 5.43 (m, 1H), 5.03 - 4.96 (m, i l l). 4.83 (d, ,7=9.5 Hz, 1 1 1 ).. 4.68 - 4.55 (m, 1 1 1 ), 4.29 - 4.21 (m, 1 1 1 ).. 4.1 5 - 4.10 (m, H i), 4.09 - 4 02 (m, H I ), 4.00 - 3.93 (m, 2H), 3.84 - 3.77 (m, 2H), 3.60 - 3.52 (m, 1H), 3.48 - 3.42 (m, 1H), 3.32 - 3.26 (m, 1H), 3.25 (s, 3H); Analytical LC/MS (ESI) mie 581.9 | (\M 1 ) , calcd for C27H3 ίNtOd S 581.7], tR = 1.43 min (Method 1); Analytical LC/MS (ESI) m/e 582.2 | (\! · I I) . calcd for C.H L iVCLS 581.7], fe = 1.40 min (Method 2). hGal-3 ICso = 1.1

Example A3 Preparation of (2i?,3/\ J ,4«S',5i< , ,6,S)-4-(4-([l, r~biphenyl]~4-yl)-lf/~l,2,3- triazol-1 -yl)-0-(((3i?,4f?,55)-4-hydroxy-5-(4-phenyl- 177-1,2, 3-triazol-l-yl)tetrahydro-2f/- pyran-3-yl)thio)-2-(hydroxymethyl)-5-methoxytetrahydro-2//-p yran-3-ol

THF

Example A3

To a 20 mL pear shaped flask was added ((2/f3//,46(5/7,6A)~3-acetoxy-4~azido-6~ (((37?,47?,55)-4-hydroxy-5-(4-phenyl-177-l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3- yl)thio)-5-methoxytetrahydro-277-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 4- ethynyl-lj '-hiphenyl (14 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL). The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 ntg, 0.037 mmol) and sodium ascorbate (6 3 g, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na2S0 4 , filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture was added sodium meth oxide (12 pL, 0 053 mmol ) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (8.9 mg, 0.014 mmol, 48% yield). ¾ NMR. (500 MHz, DMSO-de) d 8.84 (s, 1H), 8.70 - 8.62 (m, 1H), 8.00 (d, 7=7.9 Hz, 2H), 7.87 - 7.82 (m, 21 1). 7.81 - 7.76 (m, 21 1 ).. 7.75 - 7.70 (m, 2H), 7.52 - 7.43 (m, 41 1 ).. 7.42 - 7.32 (m, 2H), 5.70 - 5 65 (m, 1H), 5.49 - 5.44 (m, 1H), 4.97 - 4.92 (m, 1H), 4.87 - 4.80 (m, 2H), 4.66 - 4.56 (m, 1H), 4.29 - 4.22 (m, 1H), 4. 16 - 4.05 (m, 2H), 4.02 - 3.93 (m, 2H), 3.86 - 3 77 (m, 2H), 3.60 - 3.52 (m, 1H), 3.43 (br s, 1H), 3.34 - 3.27 (m, 1H), 3.26 (s, 3H), 3.18 (d, .7=5.2 Hz, 1H);

Analytical LC/MS (ESI) mle 657.1 [(M+H) + , calcd for C M ! ! AVCLS 656.8], fe = 2.02 min (Method 1); Analytical LC/MS (ESI) m!e 657.2 [(M+H) + , calcd for C34H36N6Q0S 656.8], /R = 2.10 min (Method 2). hGal-3 ICso = >1 1 mM.

Example A4. Preparation of (2i?,37?,4S',57?,65')-4-(4-(3-fluorophenyl)-li7-l,2,3-tiiazo l-l- yl)-6-(((3/i,4J¾,55)-4-hydroxy-5-(4-phenyl-l//-l,2,3-triazo l-l-yl)tetrahydro-2/ -pyran-3- yl)thio)-2-(hydroxymethyl)-5-methoxytetrahydro-2/7-pyran-3-o l

Example A4

To a 20 mL pear shaped flask was added ((2f?,3f?,45,5i?,65)-3-acetoxy-4-azido-6- (((37?,4i?,5,S)-4-hydroxy-5-(4-phenyl-li7-l,2,3-triazol-l-yl )tetrahydro-2//-pyran-3- yi )thio)-5-methoxytetrahydro~27/-pyran~2-yi )methyl acetate (15 mg, 0 027 mmol) and 1 - ethynyl-3-fluorobenzene (9.6 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL). The vessel w'as evacuated and flushed with nitrogen. To this mixture was added copped I f ) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na2S0 4 , filtered and concentrated. The resultant residue w'as dissolved in Me OH (3 mL) and THF (3 mL).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (10 mg, 0.017 mmol, 60% yield). l H NMR (500 MHz, DMSQ-de) d 8.79 - 8.74 (m, 1H), 8.59 - 8.54 (m, 1 1 1 ), 7.87 ~ 7.80 (m, 2H), 7.77 - 7 71 (m, 1H), 7.70 - 7.65 (m, 1H), 7.53 - 7.42 (m, 3H), 7.38 - 7.31 (m, 1H), 7.18 - 7.10 (m, 1H), 5.61 - 5.33 (m, 1 H), 4.98 - 4.88 (m, 1H), 4.83 - 4.76 (m, 1 H), 4.66 - 4.55 (m, 1H), 4.26 - 4 19 (m, 1 H), 4.16 - 4.08 (m, 1H), 3.98 (br s, 3H), 3.80 (hr d, ,7=7.4 Hz, 2H), 3.61 - 3.55 (m, 2H), 3.31 - 3.25 (m, 1 1 1 ).. 3.24 (s, 3H); Analytical I .C7MS (ESI) mie 598.9 |(M 1 1 ) , cak'd for C2SH31FN&O6S 598.7], hi = 1.64 min (Method 1); Analytical LC/MS (ESI) m!e 599.2 [ (M i n , ca!cd tor (%! % !% AES 598.7], fe = 1.58 min (Method 2). hGal-3 ICso =

0.026 iM.

Example A5. Preparation of (2R,3i?,46',5i?,6S)-6-(((3J¾,4i?,55)-4-hydroxy-5-(4-phenyl- 1 H- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2/7-pyran-3 -yl)thi o)-2-(hydroxymethyl)-5-m ethoxy-4- (4-(thiazol-2-y 1)- 1 H- 1 ,2,3 -tri azol- 1 -yl)tetrahydro-2/7-pyran-3 -ol

To a 20 mL pear shaped flask was added ((2i?,3i?,45',5i?,65)-3-acetoxy-4-azido-6-

(((3/?,47?,5S)-4-hydroxy-5-(4-phenyl-1 /7-I ,2,3-triazol-l-yl)tetrahydro-27/-pyran-3- yl)thio)-5-mefhoxytetrahydro-2/ -pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 2- ethynylthiazole (8.7 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL).

The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg,

0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na2S0 4 , filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture 'as added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in

MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH

6-7 and concentrated. The crude product was purified by preparative HFLC (Method 6).

The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (16 mg, 0.026 mmol, 97% yield). ¾ NMR (500 MHz, DMSO-de) d 8.83 (s, 1H), 8.70 - 8.63 (m, 1H), 7.96 - 7.90 (m, 1H), 7.87 - 7.81 (m, 2H), 7.80 - 7.76 (m, 1H), 7.50 - 7.44 (m, 2H), 7 38 - 7.31 (m, 1 H), 5.68 - 5.38 (m, 1H), 5 04 - 4.95 (m, 1 H), 4.79 (d, J=9.2 Hz, 1H), 4.66 - 4.55 (m, 1H), 4.27 - 4.20 (m, 1H), 4.13 (br d, .7=9 2 Hz, 2H), 4.00 - 3 89 (m, 21 1 ), 3.84 - 3.76 (m, 2H), 3 60 - 3 49 (m, 1 1 1), 3.44 - 3.34 (m, i l l ). 3 25 (s, 3H), 3.04 - 2.94 (m, 1H); Analytical LC/MS (ESI) mie 588.2 [(M+H) + , calcd for C25H29N7O6S2 587 7], tR = 1.27 min (Method 1); Analytical LC/MS (ESI) mie 588 0 [ (M i l) calcd for C25H29N7Q6S2 587.7], fe = 1.35 min (Method 2). hGal-3 ICso = 0.49

Example A6. Preparation of (2i?,3/?,45',5i?,6S)-6-(((3i?,4i?,55)-4-hydroxy-5-(4-phenyl- l77-l,2,3-triazol-l-yl)tetrahydro-2i7-pyran-3-yl)thio)-2-(hy droxymethyl)-5-methoxy-4- (4-(pyrimidin~5-yl)~ 1 H- 1 ,2,3-triazol - 1 -yl)tetrahydro-2/7-pyran-3-ol

To a 20 mL pear shaped flask was added ((27?,37745)57?,6A)·-3-^06ΐocn-4-Ez o-6- (((3/\ J ,4i?,50)-4-hydroxy-5-(4-phenyl-li/~l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3- yl)thio)-5-methoxytetrahydro-2/7-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 5- ethynylpyrimidine (8.3 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL). The vessel w'as evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Xa.’SO i, filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (1 1 mg, 0.019 mmol, 69% yield). ¾ NMR (500 MHz, DMSO-de) 6 9.33 -

9.27 (m, 2H), 9.20 - 9.15 (m, 1 H), 9.09 - 9.04 (m, III), 8.70 - 8 62 (m, I ff), 7.88 - 7.80 (m, 2H), 7.51 - 7.43 (m, 21 1 ). 7.40 - 7.29 (m, H i). 5.73 - 5.47 (m, 1H), 5.06 - 4.99 (m, 1 1 1 ), 4.88 - 4.80 (m, H i ). 4.64 - 4.55 (m, 1 1 1 ), 4.29 - 4.21 (m, H I ), 4.16 - 4.09 (m, i l l ). 4.03 (s, 1H), 3.97 (br s, 21 1 ). 3.81 (s, 21 1). 3.59 - 3.51 (m, 1H), 3.48 - 3.42 (m, 1 1 1 ). 3.32 -

3.27 (m, 1H), 3.26 (s, 3FI), Analytical LC/MS (ESI) m/e 583.1 } ( \ 1 1 1 ) . calcd for CzcdHoNsCkS 582.6], hi = 1.12 min (Method 1); Analytical LC/MS (ESI) m/e 583.1 [ {M i l) calcd for CivTLoNsQeS 582.6], /H 1 .2 1 min (Method 2). hGai-3 ICso = 0.36 mM.

Example A7. Preparation of (2i?,3i?,45',5i?,65)-6-(((3i?,4i?,55)-4-hydroxy-5-(4-phenyl- l i7-1 ,2,3-triazol-l-yl)tetrahydro-2 /-pyran-3-yl)thio)-2-(hydroxymethyl)-5-me†hoxy-4- (4-(thiazol-5-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2/ -pyran-3 -ol

THF

Example A7

To a 20 mL pear shaped flask was added ((2R,3R,4S,5R,6S)-3-acetoxy-4-azido-6- (((3i?,4/\ J ,5 « S)-4-hydroxy-5-(4-phenyl-l//-!,2,3~triazol-l-yl)tetrah ydro-2/: -pyran~3- yl)thio)-5-methoxytetrahydro-2Z/-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 5- ethynylthiazole (8.7 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL).

The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na2S0 4 , filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH

6-7 and concentrated. The crude product was purified by preparative HFLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (8.5 rng, 0.014 mmol, 52% yield). l H NMR (500 MHz, DMSO-de) d 9 09 (s, 1H), 8.84 (s, 1H), 8.66 (s, 1H), 8.30 (s, 1H), 7.84 (br d, .7=7.3 Hz, 2H), 7.47 (t, J= 7.6 Hz,

2H), 7.39 - 7.31 (rn, 1 1 1 ).. 5.72 - 5.42 (m, 1 1 1 ), 5.01 - 4.91 (rn, H I ), 4.82 (br d, ./ 9.2 Hz,

2H), 4.65 - 4.53 (m, 1H), 4.29 - 4.21 (m, 1H), 4.16 - 4.09 (m, 1H), 4.06 - 3.99 (m, 1H),

3.99 - 3.91 (m, 2H), 3 85 - 3 75 (m, 21 1 ), 3.58 - 3.50 (m, i l l ). 3 42 - 3.36 (m, 1 1 1), 3.25

(s, 3H); Analytical LC/MS (ESI) m/e 588.1 [(M+H) ÷ , calcd for C25H29N7O&S2 587.7], 2k = 1.33 min (Method 1); Analytical LC/MS (ESI) m/e 588.1 [(M+H) + , calcd for

C25H29N7O6S2 587 7], tv = 1 26 min (Method 2) hGal-3 ICso = 0.84 mM.

Example A8. Preparation of (2i?,3i?,45',5i?,65)-6-(((3i?,4i?,55)-4-hydroxy-5-(4-phenyl- l 7/-1 ,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-2-(hydroxy methyl)-5-methoxy-4- (4-(quinoxalin-6-yl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahy dro-2//~pyran-3 -o!

Example A8

To a 20 mL pear shaped flask was added ((2A,3/?,45(5/?,6A)·-3-^06ΐocn-4-Ez o-6- (((3/\ J ,4i?,50)-4-hydroxy-5-(4-phenyl-li/~l,2,3-triazol-l -yl)tetrahydro-2//-pyran-3- yl)thio)-5-methoxytetrahydro-2i/-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 6- ethynylquinoxaline (12 mg, 0.080 mmol) followed by DMF (1 mL) and water (0.3 mL). The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 ml.). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Xa.’SO i, filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 6). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (4.4 rng, 0.007 mmol, 26% yield). l H NMR (500 MHz, DMSO-de) 6 9 17 - 9.11 (m, 1H), 9.00 - 8.96 (m, H i). 8.96 - 8.91 (m, 1H), 8.70 - 8.64 (m, 1 1 !}. 8.63 - 8.58 (m, 1 1 1 ), 8.48 - 8.43 (m, ill), 8.24 - 8.18 (m, H i), 7.87 - 7 81 (m, 2H), 7.50 - 7.44 (m, 2H), 7.40 - 7.32 (m, 1H), 5.77 - 5.45 (m, 1H), 5.06 - 4.97 (m, 1H), 4.90 - 4.81 (m, 1H),

4.68 - 4.54 (m, i l l ). 4 30 - 4 21 (m, H I ), 4.17 - 4.06 (m, 21 1 ). 4 03 - 3.93 (m, 2H), 3.88 - 3.77 (m, 2H), 3.60 - 3.52 (m, 1H), 3.48 - 3.40 (m, 1H), 3.34 - 3.30 (m, i l l), 3.29 (s, 31 !); Analytical LC/MS (ESI) m/e 633.1 [(M+H) + , calcd for CirotfcNsOcS 632.7], / = 1.40 min (Method 1), Analytical LC/MS (ESI) m!e 633 1 [(M+H) + , calcd for CboFhaNsOeS 632.7], fe = 1.44 min (Method 2). hGai-3 ICso = 0.17 mM.

Example A9 Preparation of (2/?,3/7,45,5/?,65)-6-(((3i?,4A,55)-4-hydroxy-5-(4~phenyl~ li/-l,2,3-triazol-l-yl)tetrahydro-2/7-pyran-3-yl)thio)-2-(hy droxymethyl)-5-methoxy-4-

(4-(pyridazin-4-y 1)- 1 H- 1 ,2,3 -triazol- 1 -ylitetrahy dro-2/:/-pyran~3 -ol

THF

Examp!e A9

To a 20 rnL pear shaped flask was added ((2A,3/?,45',5i?,6/?)-3-acetoxy-4-azido-6- (((3i?,4i?,55)-4-hydroxy-5-(4-phenyl-li : /-l,2,3-triazol-l-yi)tetrahydro-2i/-pyran-3- yl)thio)-5-methoxytetrahydro-2/f-pyran-2-yl)methyl acetate (15 rng, 0.027 mmol) and 4- ethynylpyridazine (8.3 mg, 0.080 mmol) followed by DMF (1 rnL) and water (0.3 mL).

The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over NaiiSCL, filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 L).

To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N FIC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 6). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (8.4 mg, 0.(314 mmol, 52% yield). ! H NMR (500 MHz, DMSO-de) d 9.76 - 9.71 (m, 1 1 1 ).. 9.31 - 9.24 (m, H i), 9.20 - 9 12 (m, H I ), 8.63 - 8.57 (m, 1H), 8 12 - 8 06 (m, 1H), 7.88 - 7.81 (m, 2H), 7.51 - 7.43 (m, 2H), 7.39 - 7.31 (m, IH), 5.07 - 4.99 (m, H i ).. 4.90 - 4.80 (m, H i ), 4.67 - 4.52 (m, H i ). 4.29 - 4.19 (m, H i), 4.17 - 4 10 (m, 1H), 4.09 - 3.94 (m, 3H), 3.82 (s, 2H), 3.58 (br d, ,7=4.8 Hz, 3H), 3.38 (s, 31 1), 3.35 - 3.30 (m, 1H); Analytical LC/MS (ESI) mie 583.4 [(M+H) + , caicd for CieHioNeOeS 582.6], fe = 1.14 min (Method 1 ); Analytical LC/MS (ESI) m!e 583.3 [(M+H) + , caicd for

C M HXsCLS 582.6], fe = 1.12 min (Method 2). hGal-3 ICso = 1.24 mM.

Example A10. Preparation of (2 ?,37?,45',5/?,65)-6-(((3i?,4/?,55)-4-hydroxy-5-(4-phenyl- 17/-l,2,3-triazol-l-yl)tetrahydro-277-pyran-3-yl)thio)-2-(hy droxymethyl)-5-methoxy-4- (4-(naphthal en -2-y 1 )- 177-1,2,3 -tri azol- 1 -yl)tetrahy dro-277-py ran-3 ~ol

Example A10

To a 20 mL pear shaped flask was added ((27f,37?,45',57?,66)-3-acetoxy-4-azido-6- (((37?,47?,5S)-4-hydroxy-5-(4-phenyl-177-l,2,3-triazol-l-yl) tetrahydro-277-pyran-3- yl)thio)-5-methoxytetrahydro-277-pyran-2-yl)methyl acetate (15 mg, 0.027 mmol) and 2- ethynylnaphthaiene (12 mg, 0.(380 mmol) followed by DMF (1 mL) and water (0.3 mL). The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(ii) sulfate pentahydrate (9.3 rng, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (0.4 mL). The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over Na?.SQ4, filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL).

To this mixture 'as added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (7.7 mg, 0.012 mmol, 46% yield). ! H NMR (500 MHz, DMSO-de) d 8.87 - 8.82 (m, 111)..8.63 - 8.57 (m, 111), 8.46 - 840 (m, 111), 8.09 - 8.03 (m, ill), 803 - 795 (m, 2H), 7.95 - 7.90 (m, 1H), 7.87 - 7.81 (m, 2H), 7.59 - 7.49 (m, 211), 7.49 - 7.43 (m,

2H), 7.38 - 7.31 (m, 111), 5.58 - 5.33 (m, 111)..5.01 - 4.93 (m, 111), 4.87 - 480 (m, 111), 4.68 - 4.57 (m, 1H), 4.31 - 4.21 (m, 111).4.19 - 4.06 (m, 2H), 4.05 - 3.95 (m, 2H), 3.86 - 3.79 (m, 2H), 3.62 - 3.57 (m, 2H), 3.39 (s, 3H), 3.37 - 3.34 (m, 111): Analytical LC./MS (ESI) m!e 631.4 ((M il) , calcd for CuaHsrNeOe.S 630.7], /R = 1.76 min (Method 1); Analytical LC/MS (ESI) m!e 631.4 [(M+H) + , calcd for CMh/NALS 630.7], fe = 1.75 min (Method 2). hGal-3 ICso = 033 mM.

Example All. Preparation of methyl 4 (1-((2A,3/7,4A57?,65)-3-1I\^GOC}'-6~(((3/<47?,55)-

4-hydroxy-5-(4-phenyl-l /-l,2,3-triazol-l-yl)tetrahydro-2//-pyran-3-yl)thio)-2-

(hydroxymethyl)-5-methoxytetrahydro-2/7-pyran-4-yl)-li/-l ,2,3-triazol-4- yl)bicyclo[2.2.2]octane-l-carboxylate

example A11

To a 20 mL pear shaped flask was added ((2/f,3^,45',5i?,66)-3-acetoxy-4-azido-6- (((3i?,4f?,55)-4-hydroxy-5-(4-phenyl-lfi r -l,2,3-triazol-l-yl)tetrahydro-2f/-pyrat-3- yi)thio)-5-methoxytetrahydro-2i7-pyran-2-yi)methyl acetate (15 mg, 0.027 mmol) and methyl 4-ethynylbicyclo[2.2.2]octane-l-carboxylate (13 mg, 0067 mmol) (Fukuda, Y. et al. WO 2013/003383) followed by DMF (1 mL) and water (0.3 mL). The vessel was evacuated and flushed with nitrogen. To this mixture was added copper(II) sulfate pentahydrate (9.3 mg, 0.037 mmol) and sodium ascorbate (6.3 mg, 0.032 mmol) dissolved in water (04 mL) The vessel was again evacuated and flushed with nitrogen and stirred. After 18 h, the mixture was diluted with EtOAc (20 mL) and filtered. The organic phase was washed with brine, dried over XarSOr filtered and concentrated. The resultant residue was dissolved in MeOH (3 mL) and THF (3 mL). To this mixture was added sodium methoxide (12 pL, 0.053 mmol) (25% w/v solution in MeOH). After stirring 45 min, the reaction was neutralized with 1 N HC1 (aq.) until pH 6-7 and concentrated. The crude product was purified by preparative HPLC (Method 5). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (1 1 mg, 0.017 mmol, 63% yield) ' l l \MR (500 MHz, DMSO-de) d 8.62 - 8.57 (m, 1H), 7.96 - 7.91 (m, 1H), 7.85 - 7.79 (m, 2H), 7.49 - 7.42 (m, 21 1). 7.38 - 7.31 (m, 1H), 5.76 - 5.30 (m, 1H), 5.00 - 4.88 (m, 1H), 4.80 - 4.75 (m, 1H), 4.73 - 4.68 (m, 1H), 4.63 - 4.56 (m, 1 H), 4.24 - 4.16 (m, 1H), 4. 13 - 4.07 (m, ! H), 3.97 - 3.85 (m, 2H), 3.82 - 3.74 (m, 1H), 3.71 (s, 3H), 3.58 (s, 1H), 3.54 - 3.46 (m, 2H), 3.29 - 3.21 (m, 1H),

3.10 (s, 3H), 1.80 (s, 12H); Analytical Li ' /MS (ESI) m!e 670 9 [ {M 1 1 ) , calcd for C oi I PX OXS 670.8], hi = 1.64 min (Method 1); Analytical LC/MS (ESI) m!e 671.2 | (\! · 1 1) . calcd for C^HUiNeOsS 670.8], fe = 1.62 min (Method 2). hGal-3 ICso = 1.86

Example A12. Preparation of 3,4,5-trifluoro-A r -((2/?,3/(4 ,5K,65)-3-hydroxy-6-

(((3f?,4A,56)-4-hydroxy-5-(4-phenyl-li/-l,2,3-triazol- l -yl)tetrahydro-2/7-pyran-3- yl)thio)-2-(hydroxymethyl)-5-methoxytetrahydro-2//-pYTan-4~y l)benzamide

Example A12

To a 10 mL pear shaped flask were added (2R,3R,4S,5R,6S)-6-(((3R,4R 5S)~4- acetoxy-5-(4-phenyl-l f/-l ,2,3-triazol-l-yl)tetrahydro-2/ -pyran-3-yl)thio)-2- (acetoxymethyl)-4-amino-5-methoxytetrahydro-2i/-pyran-3-yl acetate (28 mg, 0.041 mmol), 3,4,5-trifluorobenzoic acid (15 mg, 0.083 mmol), pyridine (10 pL, 0. 12 mmol) and DMF (1 mL). To this mixture was added 1-propanephosphonic anhydride (50% solution in EtOAc) (0.049 mL, 0.083 mmol) and the reaction was stirred under N2. After 18 h, the mixture was diluted with water (25 mL) and extracted with EtOAc (2x15 mL).

The organic phase was combined, washed with brine, dried over NarSGy filtered and concentrated. The residue was purified by preparative HPLC (Method 7). The pure fractions were combined and concentrated. The resultant residue was dissolved in THF (0.1 mL) and MeOH (0.1 mL). To this mixture was added sodium methoxide (25% w/v in MeOH) (2.8 pL, 0.012 mmol) and the reaction was stirred. After 1 h, 1 M HC1 was added until pH 7. The solvent was concentrated and the residue was purified by preparative HPLC (Method 6). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (6.2 mg, 0.010 mmol, 25 % yield). ! H NMR (500 MHz, DMSO-de) 6 8.59 (s, 1 1 1 ). 8.57 - 8.50 (m, 1H), 7.93 - 7.86 (m, 2H), 7.84 (br d, ./ 7.4 Hz, 2H), 7.46 (s, 2H), 7.38 - 7.31 (m, i l l), 5.48 (d, .7=6.5 Hz, 1H), 5.04 (d, .7=5.5 Hz, 1H), 4.66 (d, .7=9.4 Hz, 1H), 4.62 - 4.51 (m, 1H), 4.22 (br dd, J=1 1.6, 4.8

Hz, 1 H), 4.16 - 4.07 (m, 2H), 4 02 - 3.93 (m, 1H), 3.86 - 3.76 (m, 2H), 3.64 - 3.49 (m, 4H), 3.44 (s, 3H). Analytical LC/MS (ESI) m!e 611.1 [(M+H) + , calcd for

C27H29F3N4O7S 610.6], tR = 1.55 min (Method 1). Analytical LC/MS (ESI) m/e 61 1.1 [(M+H) + , calcd for C27H29F3N4Q7S 610.6], hi = 1.55 min (Method 2). hGal-3 IC50 = 1.4

Example A13. Preparation of 2,3,5,6-tetrafluoro-A r -((2f?,3i?,4S , ,5i?,6S)-3 hydroxy-6-

(((3i?,4i?,5,S)-4-hydroxy-5-(4-plienyl-li7-l,2,3-triazol- l-yl)tetrahydro-27/-pyran-3- yl)thio)-2-(hydroxymethyl)-5-methoxytetrahydro-2/7-pyran-4-y l)-4-methoxybenzamide

To a 10 mL pear shaped flask were added 2,3,5,6-tetrafluoro-4-methoxybenzoic acid (15 mg, 0.068 mmol), DMF (0.4 mΐ , 5.1 mihoΐ) and DCM (1 mL). To this mixture was added oxalyl chloride (6.0 pL, 0.068 mmol) and the reaction was stirred under N?..

After 2 h, the solvent was concentrated (at room temperature), the residue was dissolved in DCM (1 mL) and added to a solution of (2i?,3i?,45,5i?,65)-6-(((3i?,4i?,55)-4-acetoxy-

5-(4-phenyl- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-277-pyran-3 -yl)thio)-2-(acetoxymethyl)-4- amino-5-m ethoxy tetrahydro-27/-pyran-3-yl acetate (23 mg, 0.034 mmol), and DMAP

(4.2 mg, 0.034 mmol), dissolved in DCM (1 mL). The reaction was stirred under N2. After 18 h, the solvent was concentrated and the crude intermediate was purified by preparative HPLC (Method 7). The pure fractions were combined and concentrated.

The resultant residue was dissolved in THF (0.5 mL) and MeOH (0.5 mL). To this mixture was added sodium methoxide (25% w/v in MeOH) (1 7 mΐ , 0 0077 mmol) and the reaction was stirred. After 1 h, 1 M HC1 was added until pH 7. The solvent was concentrated and the residue was purified by preparative HPLC (Method 6). The pure fractions were combined, concentrated and dried in vacuo to provide the title compound (0.9 mg, 0.0014 mmol, 4 % yield). 1 H NMR (500 MHz, DMSG-de) d 9.05 - 8.95 (m,

1H), 8.71 - 8.60 (m, 1 H), 7.87 - 7.78 (m, 2H), 7.51 - 7.43 (m, 2H), 7.39 - 7.30 (m, 1H), 5.65 - 5.48 (m, 1H), 5.19 - 5.02 (m, 1H), 4.72 - 4.64 (m, 1H), 4.59 - 4.50 (m, 1H), 4.24 -

4.16 (m, 1H), 4. 10 - 4.07 (m, 2H), 4.09 (s, 3 H), 3.98 - 3 89 (m, 1 H), 3.85 - 3.72 (m, 2H), 3.64 - 3.56 (m, 1H), 3.56 - 3.46 (m, 21 1). 3.43 (s, 31 1). 2.56 - 2.53 (m, 1H). Analytical l .(VMS (ESI) m/e 658 9 [(M+H) + , ealcd for CM ! MYvCLS 658.6], fe = 1.68 min (Method 1). Analytical LC/MS (ESI) m/e 659.0 [(M+Hf, calcd for C28H30F4N4O8S 658.6], /R ::: 1.65 min (Method 2). hGal-3 ICso ::: 0.57 mM.

SECTION B

Analytical LC-MS/HPLC retention time reported for each example and intermediate uses one of the following general analytical LC-MS/HPLC conditions;

LCMS Conditions:

Method A:

Column: XB ridge BEH XP C18 (50 x 2.1) mm, 2.5mth;

Mobile phase A: 10 mM NHtOAc, Water: Acetonitrile (95:5)

Mobile phase B: 10 mM NHiOAc, Water: Acetonitrile (5:95)

Gradient ::: 0-100% B over 3 min

Temperature: 50 °C:

Flow rate; 1.1 mL/min

Detection: UV at 220 nm

Method B :

Column: XB ridge BEH XP C18 (50 x 2.1) mm, 2.5mth Mobile phase A: 0.1% TFA in Water, Acetonitrile (95:5)

Mobile phase B: 0.1% TFA in Water, Acetonitrile (5:95)

Gradient = 0-100% B over 3 min

Temperature: 50 °C

Flow rate: 1.1 mL/min

Detection: UV at 220 nm

Method C:

Column-KINETEX- XB-C18 (75 x 3mm- 2.6 p )

Mobile phase A: 10 mM NFLQAc, Water:Acetonitrile (98:2)

Mobile phase B : 10 mM NH OAc, Water: Acetonitrile (2:98)

Gradient = 20-100% B over 5 min

Flow rate: 1.5 mL/min

Detection: UV at 254 nm

Method D:

Column: Waters Acquity UPLC BEH CIS (2.1 x 50 mm), 1.7 pm Mobile phase A: 10 mM NEBOAc, Water: Acetonitrile (95:5)

Mobile phase B: 10 mM NELGAc, Water:Acetonitrile (5:95)

Gradient : = 20-90% B over 1.1 minute, then a 0.6 minute hold at 90% B Temperature: 50 °C

Flow rate: 0.7 mL/min

Detection: UV at 220 nm Method E:

Column-ZORBAX-SB-C18 (50 x 4.6 mm), 6pm

Mobile phase A: 10 mM NELOAc, Water: Acetonitrile (98:2)

Mobile phase B: 10 mM NELGAc WaterAcetonitrile (2:98)

Gradient ::: 30-100% B over 5 min

Flow rate: 1.5 mL/min

Detection: UV at 254 nm Prep-HPLC conditions:

Method A:

Column: Waters XBridge C18, (19 x 150 mm), 5 mpi particles Mobile Phase A: 10 mM ammonium acetate in water

Mobile Phase B : acetonitrile

Gradient: 15-50% B over 20 minutes, then a 5-minute hold at 100% B Flow: 15 mL/min

Method B:

Column: Inertsil ODS (250 x 19 mm), 5 pm particles

Phase A: 10 mM ammonium acetate in water

Mobile Phase B: acetonitrile

Gradient: 40-65% B over 20 minutes, then a 5-minute hold at 100% B Flow: 20 mL/min

Method C:

Column: Sunfire C18 (19 x 150 mm), 5 pm particles

Mobile Phase A: 10 mM ammonium acetate in water

Mobile Phase B: acetonitrile

Gradient: 40-45% B over 12 minutes, then a 5-minute hold at 100% B Flow: 20 mL/min

Method D:

Column: Sunfire C18 (19 x 150 mm), 5 pm particles

Mobile Phase A: 10 mM ammonium acetate in water

Mobile Phase B: acetonitrile

Gradient: 30-75% B over 12 minutes, then a 5-minute hold at 100% B Flow: 20 mL/min Method E:

Column: Sunfire C18 (19 x 150 mm), 5 pm particles

Mobile Phase A: 10 mM ammonium acetate in water Mobile Phase B: acetonitrile

Gradient: 20-60% B over 20 minutes, then a 5-minute hold at 100% B

Flow: 15 mL/min Method F :

Column: Inertsil ODS (250 x 19) mm, 5pm particles

Phase A: 10 mM ammonium acetate in water

Mobile Phase B: acetonitrile

Gradient: 40-75% B over 20 minutes, then a 5-minute hold at 100% B

Flow: 17 mL/min

Method G:

Column: YMC trart Cl 8, 19 x 150 mm, 5-pm particles

Mobile Phase A: 10 mM ammonium acetate pH 4.5 with TFA

Mob il e Phase B : ace tonitri 1 e

Gradient: 20-60% B over 15 minutes, then a 5-minute hold at 100% B

Flow: 20 mL/min

Preparation of Intermediates

Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-5-methoxy-4-(4-(3,4,5-trifluorop henyl)-lH- l,2,3-triazol-l-yl)-6-((triisopropylsilyl)thio)tetrahydro-2H -pyran-2-yl)methyl acetate

A stirred solution of ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-5-me†hoxy-4-(4- (3,4,5 -trifluorophenyl)- 1 H- 1 ,2, 3 -triazol - 1 -yl)tetrahy dro-2H-pyran-2-yl )methyl acetate

(350 mg, 0 670 mmol) in dry acetonitrile (15 mL) was purged for 10 min with argon gas. Potassium carbonate (278 mg, 2.010 mmol) was then added followed by

triisopropylsilanethiol (0.216 mL, 1.005 mmol). The reaction mixture was stirred at rt for 4 h and then concentrated under reduced pressure. The residue was extracted with CH2CI2 (3 x 30 mL), washed with water (20 mL), brine (20 tnL), dried over Na2S0 4 and concentrated. The crude residue was purified by column chromatography on silica gel (20% - 50% ethyl acetate in hexanes) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-5- methoxy-4-(4-(3,4,5-trifluorophenyl)-lH-l,2,3-triazol-l-yl)- 6-

((triisopropylsilyl)thio)tetrahydro-2Hpyran-2-yl)methyl acetate (300 mg, 0.467 mmol, 69% yield) as white solid. ¾ NMR {400M Hz, CHLOROFORM-d) d 7.76 (s, 1 H), 7.56 7.37 (m, 2 H), 5.48 (d, J= 2.5 Hz, 1 H), 4.62 (d, J= 9.2 Hz, 1 H), 4.58 (dd, J= 10.4, 3.2 Hz, 1 H), 4.26 - 4.03 (m, 3 H), 3.99 - 3 89 (m, 1 H), 3.43 (s, 3 H), 2.08 (s, 3 H), 2 04 (s, 3 H), 1.36 - 1.35 (m, 3 H), 1.17 (d, ./ 7.3 Hz, 18 H); LC/MS | M I ! l = 632.2, fa = 4.19 min (Method C).

Preparation of ethyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-4-(4-(3,4,5 - trifluorophenyl)-lH-l ,2,3-triazol-l-yl)-2-((triisopropylsilyl)thio)tetrahydro-2H- pyran-3- yl)oxy)acetate

A stirred solution of ethyl 2-(((2R,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyi)-2- bromo-4-(4-(3,4,5-trifluorophenyl)-lH-l,2,3-triazol-l-yl)tet rahydro-2H-pyran-3- yl)oxy)acetate (300 mg, 0.505 mmol) in dry acetonitrile (15 mL), w¾s purged for 10 min with argon gas. Potassium carbonate (209 mg, 1.514 mmol) was then added followed by triisopropylsilanethiol (0.163 mL, 0.757 mmol) and the reaction was stirred for 4 h at rt. The reaction mixture was then concentrated under reduced pressure. The residue was extracted with CH2CI2 (3 x 50 mL), washed with water (30 mL), brine (30 mL), dried over Na2.S0 4 and concentrated. The crude residue was purified by column

chromatography on silica gel (50% 100% ethyl acetate in hexanes) to afford ethyl 2- (((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-4-(4-(3,4,5-t rifluorophenyl)-lH-l,2,3- triazol - 1 -y l)-2-((trii sopropyl silyl)thio)tetrahydro-2H-pyran-3 -yl)oxy)acetate (80 mg, 0.078 mmol, 16% yield) as a colorless liquid. ¾ NMR (400MHz, CHLOROFQRM-d) d 7.85 (s, 1 H), 7.47 - 7 35 (m, 2 H), 5 51 (d, J= 2.5 Hz, 1 H), 4.78 - 4.62 (m, 2 H), 4.55 - 4.40 (m, 1 H), 4.19 - 4.05 (m, 4 H), 4.03 - 3.82 (m, 3 H), 2.07 (s, 3 H), 2.04 (s, 3 H), 1.42 - 1.21 (m, 3 H), 1.20 - 1.06 (m, 18 H), LC/MS |\i · H | = 704 2, fe = 4 25 min (Method C).

Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)-lH-l,2,3-t riazol-l- yl)-5-methoxy-6-((triisopropylsilyl)thio)tetrahydro-2H-pyran -2-yl)methyl acetate

A stirred solution of ((2R,3R,4S,5R,6R)-3-acetoxy-6-bromo-4-(4-(3- fluorophenyl)-lH-l,2,3-triazol-l-yl)-5-methoxytetrahydro-2H- pyran-2-yl)methyl acetate (400 mg, 0.823 mmol) in dry acetonitrile (15mL) was purged with argon gas for 10 min. Potassium carbonate (341 mg, 2.468 mmol) was added followed by triisopropylsilanethiol (0.216 mL, 1.005 mmol) and the reaction was stirred for 4 h at rt. The reaction mixture was concentrated under reduced pressure. The residue was extracted with CH2CI2 (3 x 30 mL), washed with water (20 mL), brine (20 mL), dried over NaiSO-s and concentrated.

The crude residue was purified by column chromatography on silica gel (20% -- 50% ethyl acetate in hexanes) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)- lH-l,2,3-triazol-l-yl)-5-methoxy-6-((triisopropylsilyl)thio) tetrahydro-2H-pyran-2- yl)methyi acetate (70 mg, 0. 108 mmol, 13% yield) as white solid. ¾ NMR (400MHz, CHLOROFORM-d) d 7.80 (s, 1 H), 7.60 - 7.47 (m, 2 H), 7.39 (d, J= 6.0 Hz, 1 H), 7.03 (d, J = 1 2 Hz, 1 H), 5.50 (d, ./ 2.4 Hz, 1 H), 4.64 - 4 54 (m, 2 H), 4 19 - 4 06 (m, 3 H), 3.97 (d, ./ 6.4 Hz, 1 H), 3.43 (s, 3 H), 2.08 (s, 3 H), 2.06 (s, 3 H), 1 39 - 1 21 (m, 21 H); [NMR having EtOAc as solvent residue peak]; LC/MS [M+H] f = 596.2, & = 4.29 min (Method C)

Preparation of (-)-tert-butyl (1 S,5S,6R)-5-(4-(3-fluorophenyl)-lH-I,2,3-triazol-l-yl)-7- oxa-3-azabicyclo[4.1 0]heptane-3-carboxylate p Step-3

racemate, racemate, racemate, relative stereochemistry relative stereochemistry relative stereochemistry

Peak-1 Peak-2

(desired) (undesired)

Absolute stereochemistry determined by SXRD

Step 1. Preparation tert-butyl (l S,5R,6R)-5-hydroxy-7-oxa-3-azabicyclo[4. I .0]heptane-

3-carboxylate

To a solution of /V-Boc-3-hydroxy-l,2,3,6-tetrahydropyridine (500 mg, 2.51 mmol) in CH2CI2 (25 mL) was added sodiu bicarbonate (211 mg, 2.51 mmol) followed by mCPBA (928 mg, 3.76 mmol) in CH2CI2 (3 mL) at 0 °C under nitrogen. The reaction mixture was allowed to warm to roo temperature and was stirred for 24 h. The reaction mixture was diluted with CH2CI2 (100 mL), filtered through a Celite pad and the filtrate was washed with saturated Na^SCh (2x50 mL), saturated NaHCCh (2x50 mL) and saturated NaCl (25 mL). The organic layer was dried over NaiSOr, filtered, and concentrated and the crude residue w¾s purified by silica gel chromatography (40% > 50% ethyl acetate in hexanes) to yield tert-butyl (1 S,5R,6R)-5-hydroxy-7-oxa-3- azabicyclo[4. l .0]heptane-3-carboxylate (430 mg, 2.002 mmol, 80% yield) as white solid. 1 H NMR (400MHz, CHLOROFORM-d) 6 4.01 (br.s, 1 H), 3.92 - 3 44 (m, 5 H), 3 10 (dd, ,/ = 12.8, 7.2 Hz, 1 H), 1.29 (s, 9 H) (rotameric mixture).

Step 2. Preparation tert-butyl (l S,5S,6R)-5-azido-7-oxa-3-azabicyclo[4. l .0]heptane-3- carboxylate

To a stirred solution of (l S,5R,6R)-tert-butyl 5-hydroxy-7-oxa-3- azabicyclo[4. l .0]heptane-3-carboxylate (325 mg, 1.510 mmol) in THF (50 mL) was added triphenylphosphine (792 mg, 3.02 mmol). The reaction mixture was cooled 0 °C. DEAD (0.478 mL, 3.02 mmol) and DPPA (0.651 mL, 3.02 mmol) were added sequentially under nitrogen. The reaction mixture was allowed to warm to room temperature and was stirred for 16 h. The solvent was removed under reduced pressure and the crude residue was purified by silica gel chromatography (10% 15% ethyl acetate in hexanes) to yield tert-butyl (lS,5S,6R)-5-azido-7-oxa-3- azabicyelo[4.1.Q]heptane-3-earboxylate (230 mg, 0.957 mmol, 64% yield) as pale yellow oil. ¾ NMR (400MHz, DMSO-d6) d 4.09 - 3.82 (m, 2 H), 3.62 - 3.38 (m, 4 H), 3.17 (dd, J --- 13.1 , 3.3 Hz, 1 H), 1 40 (s, 9 H) (rotamerie mixture)

Step 3 Preparation tert-butyl (lS,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-7- oxa-3-azabicyclo[4.1 0]heptane-3-carboxylate

To a solution of (lR,5R,6S)-tert-butyl 5-azido~7-oxa-3-azabieyclo[4.1.0]heptane- 3-carboxylate (1.6 g, 6.66 mmol) in DMF (30 mL) and water (7.50 mL), was added sodium ascorbate (1.319 g, 6.66 mmol), copper(II) sulfate pentahydrate (1.496 g, 5.99 mmol) and 3 -fluorophenyl acetylene (3 08 mL, 26.6 mmol) sequentially at rt. The reaction mixture was degassed with N: for 5 min and heated to 85 °C for 30 min. The mixture was cooled to rt, diluted with ice cold w'ater (100 mL) and stirred for 15 min to get a solid. The solid was filtered, suspended in CH2CI2 (100 mL), filtered through celite pad and washed with excess CH2CI2. The filtrate was dried over sodium sulfate and concentrated. The residue was purified by silica gel (40% 80% ethyl acetate in hexanes) to afford tert-butyl (lS,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-7- oxa-3-azabicyclo[4.1.0]heptane-3-carboxylate (1.1 g, 3.03 mmol, 46% yield) as white solid. ¾ NMR (400MHz, CHLOROFORM-d) d 7.83 (hr s.. 1 H), 7.59 - 7.55 (m, 2 H), 7.42 - 7.38 (m, 1 H), 7.10 - 7.07 (m, 1 H), 5.18 - 5.06 (m, 1 H), 4.34 - 4 29 (m, 1 H), 3.93

- 3.45 (m, 5 H), 1.47 - 1.11 (m, 9 H) (rotamerie mixture): LC/MS, [M+H] + = 361.0, ΐk = 2.01 min (Method E).

Step 4 Preparation (-)-tert-butyl ( 1 S,5 S,6R)-5-(4-(3-fluorophenyl)- 1 H- 1 ,2,3-triazol- 1 - yl)-7-oxa-3-azabicyclo[4.1.0]heptane-3-carboxylate

Peak-1 (homochiral) Peak-2 (homochiral) racemate (desired) (undesired)

A racemic mixture of compound A (1.1 g) from the above was separated by- preparative SFC chiral chromatography using the conditions described below

Preparative chiral HPLC conditions:

Preparative Column: Chiralpak AD-FI (250 x 30 mm), 5um

BPR pressure; 100 bars

Temperature: 30 °C

Flow rate: 120 g/min

Mobile Phase; CO?/ 0.2% DEA in MeOH (60/40)

Detector Wavelength: 242 nm

Separation Program: stack injection

Injection: 0.6 mL with cycle time: 2.80 mins

Sample preparation: 1.1 g / 40 ml, MeOH:THF (1 : 1), 27.5 mg. in! .

Peak-1 has taken for subsequent steps

Analytical chiral HPLC conditions:

Analytical Column: Chiralpak AD-H (250 x 4.6 mm), Sum

BPR pressure: 100 bars

Temperature: 30 °C

Flow rate: 4 g/min

Mobile Phase: CO?./ 0.2% DEA in MeOH (50/50)

Detector Wavelength: UV 200-400 rim Peak 1 (desired): 0.3 g: chiral HPLC /R = 2 45 min; [of 22 -72.0, (c = 0.1 , MeOH).

Peak 2 (undesired): 0.5 g: chiral HPLC /R = 3.07 min; [CX]D 22 +58.0, (c = : 0.1, MeOH).

Preparation of 1 -(( 1 S, 5 S,6R)- 5 -(4-(3 -fluorophenyl)- 1H- 1 ,2,3-triazol- 1 -yl)-7-oxa-3- azabicyclo[4.1 0]heptan-3-yl)ethan-1 -one

Peak-1

Step 1. Preparation of (l S,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-7-oxa- 3- azabicyelo[4.1.Qjheptane

To stirred solution of tert-butyl (l S,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3- triazol-l-yl)-7-oxa-3-azabicyclo[4.1.0]heptane-3-carboxylate (200 mg, 0.555 mmol) in CH2CL2 (10 mL). was added activated 4 A MS (0.4g) and BFsOEt? (0.211 mL, 1.665 mmol) dropwise at 0 °C. The mixture was stirred for 15 min at at 0 °C. The reaction mixture was then quenched with MeOH (2 mL), diluted with CH2CI2 (30 mL), washed with water, 10% NaHCOs, dried over Na2S0 4 and concentrated to give (l S,5S,6R)-5-(4- (3-fluorophenyl)- !H-l,2,3-triazol-l-yl)-7-oxa-3-azabicyclo[4.1.0]heptane (110 mg, 0.335 mmol, 60% yield) as white solid which was taken to the next step without further purification. LC/MS | M i ! ] = 261.2, tn = 0.86 min (Method D).

Step 2

To a stirred solution of (l S,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-7- oxa-3-azabicyclo[4.1.0]heptane (110 mg, 0.423 mmol) in CH2CI2 (4 mL), was added triethylamine (0.177 mL, 1.268 mmol), acetic anhydride (0.060 mL, 0.634 mmol), and DMAP (5.16 mg, 0.042 mmol) sequentially at it and the mixture was stirred for 16 h.

The reaction mixture was extracted with CH2CI2 (3 x 30 mL), washed with water, brine, dried over Na2S0 4 and concentrated to give l-((lS,5S,6R)-5-(4-(3-fluorophenyl)-lH- 1 ,2,3-triazol-l-yl)-7-oxa-3-azabicyclo[4.1 0]heptan-3-yl)ethan-l -one (100 mg, 0.32 mmol, 75% yield) as brown liquid which was taken to the next step without further purification. LC/MS [M+H] = 303.0, /R ::: 1.30 min (Method C).

Preparation of final products

Example Bl. Preparation of (3S,4R,5R)-3-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-5- (((2S,3R,4S,5R,6R)-4-(4-(3-fluorophenyl)-lH-l ,2,3-triazol-l-yl)-5-hydroxy-6- (hydroxymethyl)-3-methoxytetrahydro-2H-pyran-2-yl)thio)piper idin-4-ol

Example B1

Step 1. Preparation oftert-butyl (3R,4R,5S)-3-(((2S,3R,4S,5R,6R)-5-acetoxy-6- (acetoxymethyi)-3-methoxy-4~(4-(3, 4, 5-tri fluorophenyl)- 1H-1, 2, 3-triazol-l-yl)tetrahydro- 2H-pyran-2-yl)thio)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l -yl)-4-hydroxypiperidine-l- carboxylate

To a stirred solution ((2R,3R,4S,5R,6S)-3-acetoxy-5-methoxy-4-(4-(3,4,5- trifluorophenyl)-lH-l,2,3-triazol-l-yl)-6-((triisopropylsily l)thio)tetrahydro-2H-pyran-2- yl)methyl acetate (20 mg, 0.032 mmol) in acetonitrile (2 mL), was added tert-buty! (lS,5S,6R)-5-(4-(3-fluorophenyl)-lH-l ,2,3-triazol-l-yl)-7-oxa-3- azabicyclo[4.1.0]heptane-3-carboxylate (1 1.41 mg, 0.032 mmol) in acetonitrile (2 mL). The solution was purged with argon for 10 ruin. TBAF (0.032 mL, 0.032 mmol) was added dropwise and the mixture was stirred for 10 min at rt under argon. The solvent was removed under reduced pressure to afford tert-butyl (3R,4R,5S)-3-(((2S,3R,4S,5R,6R)-5- acetoxy-6-(acetoxymethyl)-3-methoxy-4-(4-(3,4,5-trifluorophe nyl)-lH-l ,2,3-triazol-l- yl)tetrahy dro-2H-pyran-2-yl)thio)-5-(4-(3-fluorophenyl)- 1H- 1 ,2,3-triazol- 1 -yl)-4- hydroxypiperidine-l -carboxylate (30 mg, 0.020 mmol, 65% yield) which was taken to the next step without further purification. LC/MS [M+H] + = 836.0, ΐk = 3.47 min (Method

C).

Step 2

To a solution of tert-butyl (3R,4R,5S)-3-(((2S,3R,4S,5R,6R)-5-acetoxy-6- (acetoxymethyl)-3-methoxy-4-(4-(3,4,5-trifluorophenyl)-lH-l, 2,3-triazol-l -yl)tetrahydro- 2H-pyran-2-yl)thio)-5-(4-(3 -fluorophenyl)- 1H- 1 ,2,3 -tri azol- 1 -yl)-4-hy dr oxypiperidine- 1 - carboxylate (30 mg, 0.036 mmol) in CH2CI2 (5 mL) was added TEA (2 77 pL, 0.036 mmol) and the reaction mixture was stirred for 1 h. The solvent was then removed under reduced pressure. The residue was dissolved in methanol (2 mL) and sodium methoxide (0 194 mg, 3.59 mthoΐ) was subsequently added. The mixture was stirred for 30 min at rt. The reaction mixture was acidified with AcOH (0.1 mL) and was concentrated and purified by preparative HPLC (Method A) to afford (3S,4R,5R)-3-(4-(3-fluorophenyl)- lH-l,2,3-triazol-l-yl)-5-(((2S,3R,4S,5R,6R)-4-(4-(3-fluoroph enyl)-lH-l,2,3-triazol-l- yl)-5-hydroxy-6-(hydroxymethyl)-3-methoxytetrahydro-2H-pyran -2-yl)thio)piperidin-4- ol (3 mg, 4.47 pmol, 13% yield) (Example Bl). Ή NMR (400 MHz, METH AN OL- d4) d 8.66 (s, 1 H), 8.53 (s, 1 H), 7.70 - 7.60 (m, 4 H), 7.52 -7 46 (m, 1 H), 7 15 - 7.10 (m, 1 H), 4.94 - 4.80 (m, 3 H), 4.19 - 4.12 (m, 3 H), 3.89 - 3.76 (m, 6 H), 3.45 - 3 32 (m, 2 H), 3.32

(s, 3 H, obscured with solvent peak); LC/MS [M+H] f = 652.3, (R = 1.63 min (Method A). hGa.13 ICso = 0.067 uM.

Example B2. Preparation of (3 S,4R,5R)~3 -(4-(3 -fluorophenyl)- 1 H- 1 ,2,3 -tri azol- 1 -yl)-5- (((2S,3R,4S, 5R,6R)-4-(4-(3-fluorophenyl)- 1 H- 1 ,2,3-triazol- 1 -yl)-5-hydroxy-6-

(hydroxymethyl)-3-methoxytetrahydro-2H-pyran-2-yl)thio)pi peridin-4-ol

Example B2

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-I-acetyl-5-(4-(3- fluorophenyl)-lH-l,2,3-triazol-l -yl)-4-hydroxypiperidin-3-yl)thio)-5-methoxy-4-(4- (3,4,5 -trifluoropheny 1 )- 1 H- 1 ,2, 3 -tri azol- 1 -yl)tetrahy dro-2H-pyran-2-yl)methy 1 acetate

To a stirred solution ((2R,3R,4S,5R,6S)-3-acetoxy-5-methoxy-4-(4-(3,4,5- trifluorophenyl)-lH- 1,2, 3-tri azol- l-yl)-6-((triisopropylsilyl)thio)tetrahydro-2H-pyran-2- yl)methy! acetate (30 mg, 0.047 mmol) in acetonitrile (2 niL), was added l-((lS,5S,6R)- 5-(4-(3-fluorophenyl)-lH-l, 2, 3-tri azol- l-yl)-7-oxa-3-azabicyclo[4.l 0]heptan-3-yl)ethan- l-one (14.36 mg, 0.047 mmol) in acetonitrile (2 mL). The solution was purged with argon for 10 min. TBAF (0.032 mL, 0.032 mmol) was then added dropwise and the mixture was stirred for 10 min at rt under argon. The solvent was removed under reduced pressure and the product was purified by preparative HPLC (Method C) to afford ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-l-acetyl-5-(4-(3- fluorophenyl)-lH-l,2,3- triazol-l-yl)-4-hydroxypiperidin-3-yl)thio)-5-methoxy-4-(4-( 3,4,5-trifluorophenyl)-lH- l,2,3-triazol-l-yl)tetrahydro-2H-pyran-2-yl)methyl acetate (8 mg, 10.29 pm of 22% yield) as white solid. LC/MS, [M+H] = 778.2, <¾ = 2.68 min (Method C). Step 2

To a stirred solution of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-l-acetyl-5- (4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)-4-hydroxypiperidi n-3-yl)thio)-5-methoxy-4- (4-(3,4,5-trifluorophenyl)-lH-l,2,3-triazol-l-yl)tetrahydro- 2H-pyran-2-yl)methyl acetate (7 mg, 9.00 pmoi) in methanol (2 mL) was added sodium methoxide (25% wt in methanol) (1.945 mg, 9.00 pmol) and the mixture was stirred for 30 min at rt. The reaction mixture was acidified with AcOH (0.1 mL), concentrated and purified by preparative HPLC (Method A) to afford ((3S,4R,5R)-3-(4-(3-fluorophenyl)-lH-I,2,3- triazol-l-yl)-5-(((2S,3R,4S,5R,6R)-4-(4-(3-fluorophenyl)-lH- l,2,3-triazol-l -yl)-5- hydroxy-6-(hydroxymethyl)-3-methoxytetrahydro-2H-pyran-2-yl) thio)piperidin-4-ol (2.3 mg, 3.32 pmol, 37 % yield) (Example B2). 1 H NMR (400 MHz, METHANOL-d4) d 8.75, 8.70* (s, 1 H), 8.52 (s, 1 H), 7.77 - 7.63 (m, 4 H), 7.51 - 7.45 (m, 1 H), 7.13 - 7.07 (m, 1 H), 4.96 - 4.94 (m, 1 H), 4.77 (d, ./ 9.2 Hz, 1 H), 4 58 - 4 48 (m, 2 H), 4 26 - 4.17 (m, 1 H), 4.15 - 4.05 (m, 2 H), 3.86 -3.77 (m, 3 H), 3.76 - 3.71 (m, 1 H), 3.45 - 3.37 (m, 3 H), 3.32 (s, 3 H, obscured with solvent peak), 2.31, 2.23* (s, 3H) (*Rotameric mixture);

LC/MS [M+H] + = 694.3, fe = 1.71 min (Method A). hGal3 ICso = 0.037 mM.

Example B3. Preparation of l-((3S,4R,5R)-3-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l -yl)-

5-(((2S,3R,4S,5R,6R)-4-(4-(3-fluorophenyl)-lH-I,2,3-triaz ol-l-yl)-5-hydroxy-6- (hy droxym ethyl)-3 -methoxytetrahy dro-2H-py ran-2-yl)thi o)-4-hy droxypiperi di n- 1 - yl)ethan-l-one

Example B3

Step 1. Preparation of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-I-acetyl-5-(4-(3- fluorophenyl)-lH-l,2,3-triazol-1 -yl)-4-hydroxypiperidin-3-yl)thio)-4-(4-(3- fluorophenyl)-lH-l,2,3-triazol-l-yl)-5-methoxytetrahydro-2H- pyran-2-yl)methyl acetate To a stirred solution ((2R,3R,4S,5R,6S)-3-acetoxy-4-(4-(3-fluorophenyl)-lH- l,2,3-triazol-l-yl)-5-methoxy-6-((triisopropylsilyl)thio)tet rahydro-2H-pyran-2-yl)methyl acetate (20 mg, 0.034 mmol) in acetonitrile (2 rnL) was added l-((lS,5S,6R)-5-(4-(3- fluorophenyl)-lH-l,2,3-triazol-l-yl)-7-oxa-3-azabicyclo[4.1. 0]heptan-3-yl)ethan-l-one (10.15 mg, 0.034 mmol) in acetonitrile (2 mL). The solution was purged with argon for

10 min. TBAF (0.034 mL, 0.034 mmol) was then added dropwise and the mixture was stirred for 10 min at rt under argon. The solvent was removed under reduced pressure. The residue was purified by prep-HPLC (Method B) to afford ((2R,3R,4S,5R,6S)-3~ acetoxy-6-(((3R,4R,5S)-l-acetyl-5-(4-(3-fluorophenyl)-IH-l,2 ,3-triazol-l-yl)-4- hydroxypiperidin-3-yl)thio)-4-(4-(3-fluorophenyl)-lH-l,2,3-t riazol-l-yl)-5- methoxytetrahydro-2H-pyran-2-yl)methyl acetate (7 mg, 9.44 pmol, 28% yield) as white solid. LC/MS jM ! I | = 742.0, /: < = 2.40 min (Method C). Step 2

To a stirred solution of ((2R,3R,4S,5R,6S)-3-acetoxy-6-(((3R,4R,5S)-l-acetyl-5- (4-(3 -fluorophenyl)- 1H- 1 ,2,3 -triazol- 1 -yl)-4-hydroxypiperi din-3 -yl)thio)-4-(4-(3 - fluorophenyl)- 1H-1, 2, 3-triazol-l-yl)-5-methoxytetrahydro-2H-pyran-2-yl)methyl acetate (7 mg, 9.44 pmol) in methanol (2 mL) was added sodium methoxide (25% wt in methanol) (2.0 mg, 9.44 pmol) and the mixture was stirred for 30 min at rt. The reaction mixture was acidified with AcOH (0.1 mL), concentrated, and purified by preparative HPLC (Method A) to afford l-((3S,4R,5R)-3-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)- 5-(((2S,3R,4S,5R,6R)-4-(4-(3-fluorophenyl)-lH-l ,2,3-triazol-l-yl)-5-hydroxy-6- (hydroxymethyl)-3 -methoxytetrahydro-2H-pyran-2-yl)thio)-4-hydroxypiperidin- 1 - yl)ethan-l-one (2.76 mg, 4.11 pmol, 44% yield) (Example B3). X H NMR (400 MHz, METHANOL-d4) d 8.58, 8.53* (s, 1 H), 8.39 (s, 1 H), 7.60 - 7.39 (m, 4 H), 7.38 - 7.01 (m, 2 H), 7.01 - 6. 95 (m, 2 H), 4 83 - 4.78 (m, 2 H), 4 65 - 4.47 (m, 1 H), 4.41 - 4.01 (m, 3 H), 3.99 - 3.93 (m, 2 H), 3.77 - 3.62 (m, 4 H), 3.38 - 3.03 (m, 2 H), 3.32, 3,25* (s, 3 H, obscured with solvent peak), 2.19, 2.1 1 * (s, 3H) (*Rotameric mixture), LC/MS [M+H] = 658.0, hi = 1.86 min (Method C). hGa!3 ICso = 0.054 uM

Example B4. Preparation of 2-(((2S,3R,4S,5R,6R)-2-(((3R,4R,5S)-l-acetyl-5-(4-(3- fluorophenyl)- 1H- 1 ,2,3-triazol- 1 -yl)-4-hydroxypiperidin-3-yl)thio)-5-hydroxy-6- (hydroxyniethyl)-4-(4-(3,4,5-trifluorophenyl)-lH-l ,2,3-triazol-l-yl)tetrahydro-2H-pyran-

3-yl)oxy)acetic acid

Step 1. Preparation of ethyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-2- (((3R,4R, 5 S)- 1 -acetyl-5~(4-(3 -fluorophenyl)- 1H- 1 , 2,3 -triazol - 1 -y 1 )-4-hydroxypi peri din-3 - yl)thio)-4-(4-(3,4,5-trifluorophenyl)-lH-l,2,3-triazol-l-yl) tetrahydro-2H-pyran-3- yl)oxy)acetate

To a stirred solution ethyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyl)-4- (4-(3 ,4,5 -tri fl uorophenyl)- 1 H- 1,2,3 -triazol - 1 -y l)-2-((tri i sopropyl silyl)thi o)tetrahydro-2H- pyran-3-yl)oxy)acetate (40 mg, 0.057 mmol) in acetonitrile (2 mL) was added

((lS,5S,6R)-5-(4-(3-fluorophenyl)-lH-l,2,3-triazol-l-yl)- 7-oxa-3- azabicyclo[4.1.0]heptan-3-yl)ethan-l-one (17.18 rng, 0.057 mmol) in acetonitrile (2 mL). The solution was purged with argon for 10 min. TBAF (0.057 mL, 0.057 mmol) was then added dropwise and the mixture was stirred for 10 min at rt under argon. The solvent was removed under reduced pressure. The residue was purified by preparative HPLC

(Method D) to afford ethyl 2-(((2S,3R,4S,5R,6R)-5-aeetoxy-6-(acetoxymethyl)-2-

(((3R,4R,5S)-l-acetyi-5-(4-(3-fluorophenyl)-lH-l,2,3-tria zol-l-yl)-4-hydroxypiperidin-3- yl)thio)-4-(4-(3, 4, 5-trifl uorophenyl)- 1H- 1,2, 3-triazol-l-yl)tetrahydro-2H-pyran-3- yl)oxy)acetate (12 mg, 0.013 mmol, 23% yield) as white solid. LC/MS [M+H] + = 850.2, in ------ 2.82 min (Method C).

Step 2

To a stirred solution of ethyl 2-(((2S,3R,4S,5R,6R)-5-acetoxy-6-(acetoxymethyJ)-

2-(((3R,4R,5 S)-l -acetyl-5-(4-(3-fluorophenyl)- 1H- 1 ,2,3-triazol- 1 -yl)-4-hy droxypiperidin- 3 -yl)thio)-4-(4-(3 ,4,5-trifluorophenyl)- 1 H- 1 ,2,3 -triazol- 1 -yl)tetrahydro-2H-pyran-3 - yl)oxy)acetate (11 mg, 0.013 mmol) in THF (2 mL), was added water (0.500 mL) and

Li OH (0.930 rng, 0.039 mmol) at rt and the mixture was stirred for 1 h. The solvent was then removed under reduced pressure and the residue was purified by preparative HPLC (Method E) to afford 2-(((2S,3R,4S,5R,6R)-2-(((3R,4R,5S)-l-acetyl-5-(4-(3- fluorophenyl)-lH-l,2,3-triazol-l-yl)-4-hydroxypiperidin-3-yl )thio)-5-hydroxy-6- (hydroxymethyl)-4-(4-(3,4,5-trifluorophenyl)-lH-l,2,3-triazo l-l-yl)tetrahydro-2H-pyran-

3-yl)oxy)acetic acid (2.3 mg, 3.09 pmol, 24% yield) (Example B4). Ή NMR (400 MHz, METHANOL-d4) d 8.69, 8 65* (s, 1H), 8 53, 8 52* (s, 11 1).. 7.69 - 7.60 (m, 4 H), 7.50 -

7.43 (m, 1 H), 7.13 - 7.06 (m, 1 H), 5.15 - 4.94 (m, 2 H), 4.95 (d, J= 9.2 Hz, 1 H), 4 59 - 4.42 (m, 5 H), 4.17 - 4.06 (m, 3 H), 3.93 - 3.74 (m, 4 H), 3.50 - 3.44 (m, 1 H), 2.30, 2.23* (s, 3 H) (*Rotameric mixture), LC/MS [M-H/G = 736.0, /R = 1.1 1 min (Method C). hGal3 ICso = 0.032 mM.

It will be evident to one skilled in the art that the present disclosure is not limited to the foregoing illustrative examples, and that it can be embodied in other specific forms without departing from the essential attributes thereof. The examples therefore should be considered in all respects as illustrative and not restrictive, reference being made to the appended claims, rather than to the foregoing examples, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced.