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Title:
THERMOPHILIC LONG CHAIN ALKANOL DEHYDROGENASE AND ITS ENCODING GENE AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2009/030074
Kind Code:
A1
Abstract:
An alcohol dehydrogenase, which is isolated from Geobacillus thermodenitrificans NG80-2, consists of 387 amino acids shown in SEQ ID NO: 2. The enzyme has ability to degrade long chain alkanol of chain length longer than C16. It can be widely act on long chain alkanol of chain length between C12 and C30, and optimum substrate is octyl alcohol. A gene encoding the enzyme, a recombinant plasmid pET-28a (+) expressing the enzyme, and a recombinant Escherichia coli BL21 producing the same are further provided.

Inventors:
WANG LEI (CN)
LIU XUEQIAN (CN)
FENG LU (CN)
Application Number:
PCT/CN2007/002825
Publication Date:
March 12, 2009
Filing Date:
September 26, 2007
Export Citation:
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Assignee:
UNIV NANKAI (CN)
WANG LEI (CN)
LIU XUEQIAN (CN)
FENG LU (CN)
International Classes:
C12N15/53; C02F3/34; C12N1/20; C12N9/04; C12N15/63; C12N15/70; C02F101/30; C02F101/32; C12R1/07
Foreign References:
CN1366550A2002-08-28
CA2510657A12007-01-18
Other References:
DATABASE GENBANK [online] 29 March 2007 (2007-03-29), Database accession no. CP000557
DATABASE GENBANK [online] 19 May 2007 (2007-05-19), Database accession no. BAD77210
LU FENG ET AL.: "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from deep-subsurfcae oil reservoir", PNAS, vol. 104, no. 13, 27 March 2007 (2007-03-27), pages 5602 - 5607, XP007915388, DOI: doi:10.1073/pnas.0609650104
HIROAKI NAGASHIMA ET AL.: "Long-chain n-alkanol dehydrongenase from Pseudomonas putida", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 82, no. 4, 1996, pages 328 - 333
Attorney, Agent or Firm:
LIAN & LIEN IP ATTORNEYS (TowerA Huaxin Building,NO.33 Anding Road, Chaoyang District, Beijing 9, CN)
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