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Title:
THREE-DIMENSIONAL CULTURE OF PRIMARY CANCER CELLS USING TUMOR TISSUE
Document Type and Number:
WIPO Patent Application WO/2018/169007
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for forming a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue as a starting material while inhibiting the proliferation of cells (e.g., fibroblasts) other than cancer cells, said method ensuring a high proliferation ability of the cell mass which contains primary cancer cells as the main component, being easy to handle and having a versatility and high throughput properties. To solve this problem, provided is a method for forming a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, said method comprising a three-dimensional culture step for culturing cells, which are obtained from the tumor tissue, in a medium containing not more than 5 v/v% of an extracellular matrix on a cell culture base material substantially having a low adhesiveness.

Inventors:
KAKINUMA HIDEAKI (JP)
SHIMADA YUKIKO (JP)
INOUE HIROAKI (JP)
MORIKAWA TAKASHI (JP)
Application Number:
PCT/JP2018/010247
Publication Date:
September 20, 2018
Filing Date:
March 15, 2018
Export Citation:
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Assignee:
LSI MEDIENCE CORP (JP)
International Classes:
C12N5/09; C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
WO2017146122A12017-08-31
WO2016047801A12016-03-31
WO2011077894A12011-06-30
WO2016047801A12016-03-31
Foreign References:
Other References:
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VINCI M ET AL.: "Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation", BMC BIOLOGY, vol. 10, no. 1, 22 March 2012 (2012-03-22), XP021125315
See also references of EP 3597733A4
"Tissue Culture Technology -Basics- (Soshiki Baiyo no Gijutsu -Kisohen-)", 1996, ASAKURA PUBLISHING CO., LTD.
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Attorney, Agent or Firm:
SANUKI, Shinichi et al. (JP)
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