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Title:
A TIP SEAL ARRANGEMENT FOR INJECTORS CONTAINING STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2019/226127
Kind Code:
A2
Abstract:
An injector arrangement (100) which allows the fat tissue to be drawn through a suction tip (108), stored inside a tube (101) without intervention or exposure to air and centrifuged finally in order to obtain fat based stem cells, comprising a modular piston (103) with a movable structure which allows drawing the fat tissue into the tube (101) through the said suction tip (108) by means of a piston tip seal (102), a piston shaft (105) with modular structure which allows the said modular piston (103) to move inside the tube (101) in forward and backward directions and which can be separated from the modular piston (103) in order to allow the tube (101) to be placed inside the present centrifuge device after the fat tissue is drawn inside the tube (101), and an O-ring (104) which engages into the O-ring seat (1033) formed on the hollow locking part (1031) with independent structure in order to make the hollow locking part (1031) with an independent structure introduced on the said modular piston (103) to act as a locking device during the centrifuge, or in other words, to prevent the fat tissue and split stem cells to leak outside the tube (101) during the centrifuge, wherein; it allows stem cells to be collected on the piston tip seal (102) with a concave structure without experiencing losses during the upside down centrifuge.

Inventors:
TIRYAKI KEMAL TUNC (TR)
Application Number:
PCT/TR2018/050732
Publication Date:
November 28, 2019
Filing Date:
November 27, 2018
Export Citation:
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Assignee:
TIRYAKI KEMAL TUNC (TR)
International Classes:
C12N1/00
Attorney, Agent or Firm:
DESTEK PATENT INC. (TR)
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Claims:
CLAIMS

1. An injector arrangement (100) which allows the fat tissue to be drawn through a suction tip (108), stored inside a tube (101 ) without intervention or exposure to air and centrifuged finally in order to obtain fat based stem cells, comprising a modular piston (103) with a movable structure which allows drawing the fat tissue into the tube (101 ) through the said suction tip (108) by means of a piston tip seal (102), a piston shaft (105) with modular structure which allows the said modular piston (103) to move inside the tube (101 ) in forward and backward directions and which can be separated from the modular piston (103) in order to allow the tube (101 ) to be placed inside the present centrifuge device after the fat tissue is drawn inside the tube (101 ), and an O-ring (104) which engages into the O-ring seat (1033) formed on the hollow locking part (1031 ) with independent structure in order to make the hollow locking part (1031 ) with an independent structure introduced on the said modular piston (103) to act as a locking device during the centrifuge, or in other words, to prevent the fat tissue and split stem cells to leak outside the tube (101 ) during the centrifuge, wherein; it comprises a piston tip seal (102) with a concave structure.

Description:
A TIP SEAL ARRANGEMENT FOR INJECTORS CONTAINING STEM CELLS

Technical Field

The invention relates to an injector arrangement which allows stem cells obtained from tissues of a living organism to be transferred from their initial location to a centrifuge medium without being exposed to air or without experiencing any volume losses.

Background Art

Stem cells are obtained from embryos, fetuses or tissues of the human body such as bone marrow, blood, fat etc. Stem cells that are available in the human body are progenitor cells which only possess the ability to transform into the cells of certain tissues, in contrast to embryonic stem cells.

Today, after having discovered the effectiveness of stem cells obtained from fat tissue especially in treatment of tissue losses and regeneration, various methods have been developed for obtaining stem cells from fat tissue. Accordingly, the fat tissue located at the lower abdomen or the inner thigh area is removed using one of the liposuction techniques known in the state of the art and transferred to the laboratory. After obtaining stem cells from the fat tissue sample, the said stem cells are put through several centrifuge process before transplantation. However, the centrifuge process is carried out using a regular centrifuge tube and volume losses are experienced during the transfer of the obtained stem cells from their initial tube, injector etc. to the centrifuge tube. The aforementioned reasons, developing an injector arrangement that prevents volume losses during the centrifuge process is required.

Therefore, the aforementioned disadvantages and lack of an adequate solution to the problem have made it necessary to develop an injector arrangement which allows stem cells obtained from tissues of a human body to be transferred from their initial location to a centrifuge medium without being exposed to air or without experiencing volume losses.

Object of the Invention

The present invention relates to develop an injector arrangement which overcomes all disadvantages and provides further advantages, which allows stem cells obtained from tissues of a human body to be transferred from their initial location to a centrifuge medium without being exposed to air or without experiencing volume losses, due to the lack of an adequate solution to the problem in the prior art.

The primary object of the invention is to minimize the stem cell losses to be obtained during the centrifuge process of the split fat tissue, with the concave structure of the injector seal.

The object of the invention is to develop an injector arrangement, especially intended to be used to obtain fat tissue based stem cells, which allows stem cells obtained from the fat tissue from any preferred area of the individual’s body to be delivered to the centrifuge process without requiring them to be transferred into a separate tube or injector. When the injector are centrifuged upside down, heavier stem cells are aggregated at the bottom of the concave arrangement and therefore the stem cell losses are minimized.

Another object of the invention is to develop an injector arrangement, especially intended to be used to obtain fat tissue based stem cells, which transfers the cells to be obtained to the centrifuge medium without exposing them to air.

Another object of the invention is to develop an injector arrangement, especially intended to be used to obtain fat tissue based stem cells, which transfers the cells to be obtained to the centrifuge medium without losing their vitality.

Another object of the invention is to develop an injector arrangement, especially intended to be used to obtain fat tissue based stem cells, which does not possess the risk of infection.

Another object of the invention is to develop an injector arrangement, especially intended to be used to obtain fat tissue based stem cells, which transfers the stem cells to the centrifuge medium without experiencing volume losses.

Another object of the invention is to develop an injector arrangement, intended to be used to obtain fat tissue based stem cells, which eliminates risks such as infection and contamination.

Another object of the invention is to simplify the process by allowing the stem cells to be stored inside the injector into which they are initially drawn.

The present invention relates to an injector arrangement intended to be used to transfer the stem cells obtained from tissues of a living organism from their initial medium to the centrifuge medium without exposing them to air or without experiencing volume losses. Accordingly, the injector arrangement develop by the invention comprises a modular piston with a movable structure which allows drawing the fat tissue into the tube through a suction tip by means of a piston tip seal, a piston shaft with modular structure which allows the said modular piston to move inside the tube in forward and backward directions and which can be separated from the modular piston in order to allow the tube to be placed inside the present centrifuge device after the fat tissue is drawn inside the tube, and an O-ring which engages into the O-ring seat formed on the hollow locking part with independent structure in order to make the hollow locking part with an independent structure introduced on the said modular piston to act as a locking device during the centrifuge, or in other words, to prevent the fat tissue and split stem cells to leak outside the tube during the centrifuge, in order to allow the fat tissue to be drawn through a suction tip, stored inside a tube without intervention or exposure to air and centrifuged finally in order to obtain fat based stem cells. The injector arrangement developed by the present invention further comprises a piston tip seal with a concave structure introduced at the tip of the modular piston with movable structure.

In order to achieve the objects of the invention, a piston tip seal having a concave structure is introduced at the tip of the modular piston with movable structure mentioned in the invention. The concavity of the mentioned seal prevents the stem cells to leak in between the seal and the outer wall of the injector during the centrifuge process in order to prevent volume losses.

The structural and characteristic features and all of the advantages of the invention shall become apparent with the appended drawing and the detailed description with reference to those drawings. Therefore, assessment should be based on these drawing and the detailed description.

Brief Description of the Drawings

Figure-1 . A general perspective view of the injector arrangement of the invention.

Figure-2. An exploded perspective view of the injector arrangement of the invention.

Figure-3. An exploded perspective view of the injector arrangement of the invention from a different point of view.

Figure-3.1 Exploded and assembled detail views of the modular piston heads of the invention showing close-ups of the O-ring and the O-ring seat.

Figure-4. A view of the piston shaft while disengaged from the threaded part.

Figure-5. A general view of an embodiment where concave injector seals are used.

Figure-6,7,8. Views of an injector embodiment where concave injector seals are used from different points of view.

Figure-9, 10. Views of the concave injector seal from different points of view.

The drawings should scaled and details not necessary to understand the invention may have been omitted. In addition, at least partially identical elements or elements with at least identical functions are indicated with same numbers. Parts References

100. Injector arrangement

101. Tube

102. Piston tip seal

1021 . Positioning gap

103. Modular Piston

1031 . Hollow Locking Part

1032. Modular Piston Screw Hole

1033. O-ring seat

104. O-ring

105. Piston shaft

106. Threaded tip

107. Piston handle

108. Suction tip

109. Piston shaft seat

Detailed Description of the Invention

The present invention shall become apparent with the preferred embodiments of an injector arrangement, which allows stem cells obtained from fat tissue of a living organism, to be transferred from their initial location to a centrifuge medium without being exposed to air or without experiencing volume losses, given in the herein detailed description, without limiting the scope of the invention.

The present invention relates to an injector arrangement, whose example views are given in figures 1 -5, which allows stem cells obtained from tissues of a human body to be transferred from their initial location to a centrifuge medium without being exposed to air or without experiencing volume losses. Accordingly, the injector arrangement comprises a modular piston (103) with a movable structure which allows drawing the fat tissue into the tube (101 ) through a suction tip (108) by means of a piston tip seal (102), a piston shaft (105) with modular structure which allows the said modular piston (103) to move inside the tube (101 ) in forward and backward directions and which can be separated from the modular piston (103) in order to allow the tube (101 ) to be placed inside the present centrifuge device after the fat tissue is drawn inside the tube (101 ), and an O-ring (104) which engages into the O-ring seat (1033) formed on the hollow locking part (1031 ) with independent structure in order to make the hollow locking part (1031 ) with an independent structure introduced on the said modular piston (103) to act as a locking device during the centrifuge, or in other words, to prevent the fat tissue and split stem cells to leak outside the tube (101 ) during the centrifuge. The injector arrangement developed by the present invention further comprises a piston tip seal (102) with a concave structure introduced at the end of the said modular piston (103) with a movable structure.

The invention relates to an injector arrangement (100) which allows the fat tissue to be drawn through a suction tip (108), stored inside a tube (101 ) without intervention or exposure to air and centrifuged finally in order to obtain fat based stem cells. It comprises a modular piston (103) with a movable structure which allows drawing the fat tissue into the tube (101 ) through the said suction tip (108) and a piston shaft (105) with modular structure which allows the said modular piston (103) to move inside the tube (101 ) in forward and backward directions and which can be separated from the modular piston (103).

It comprises threaded tips (106) which allow the said modular piston shaft (105) to be separated from the modular piston (103) and a modular piston screw hole (1032) introduced on the upper part of the modular piston (103) which allows the said threaded tips (106) to be removed and fastened. In addition, the said modular piston shaft (105) comprises a piston handle (107). On the other hand, the said modular piston (103) comprises at least one sealing O-ring (104). It also comprises a piston tip seal (102) comprising a positioning gap (1021 ) which is confined by the said modular piston (103) inside the tube (101 ) and in which the modular piston (103) is centered. Furthermore, it comprises a separate hollow locking part (1031 ) with a diameter larger than the inner tube (101 ) diameter of the said modular piston (103).

The operation of the injector arrangement (100) is as follows; when the modular piston (103) is pulled towards the a-direction, stem cells are sucked or drawn into the tube (101 ) through the suction tip (108). When the modular piston (103) is pulled towards the a-direction following a linear motion, the piston tip seal (102) advances inside the tube (101 ) with a leak tight contact and generates high suction power. Once the stem cells are confined inside the tube (101 ), the piston shaft (105) moves freely inside the piston shaft seat (109) which is formed vertically at the axial center of the hollow locking part (1031 ). The modular piston shaft (105) passes through the piston shaft seat (109) and unmounted from the modular piston screw hole (1032) by means of the threaded tips (106). The modular piston shaft (105) and the modular piston (103) which are engaged by being screwed are thereby separated from each other by twisting. Once stem cells are drawn into the tube (101 ), the piston shaft (105) is separated from the modular piston (103) by twisting. Thereby, the piston tip seal (102) and the hollow locking part (1031 ) of the modular piston (103) is maintained fastened inside the tube (101 ). Thereafter the stem cells inside the tube (101 ) are isolated from the air by means of a plug and a leak tight lid at the suction tip (108). In order to ensure a strong seal inside the tube (101 ) and prevent the stem cells which are being drawn into the tube (101 ) during the centrifuge to be thrown out of the tube (101 ) due to the centrifugal force, sealing O-rings (104) are placed inside the O-ring seat (1033) introduced on the hollow locking part (1031 ). The said sealing O-ring (104) ensures the hollow locking part (1031 ) to be held fixed against the centrifugal force during the centrifuge, in addition to ensuring tightness.

The said modular piston shaft (105) engages to the modular piston (103) by means of a threaded connection. This screw connection is established by engaging the modular piston screw hole (1032) introduced on the modular piston (103) and the threaded tip (106) introduced on the piston shaft (105) by twisting. Once the stem cells are drawn into the tube (101 ), the said piston shaft is removed by twisting before commencing the centrifuge process. Thereby, the length of the injector (100) is reduced in order to fit into the centrifuge. On the other hand, depending on the choice of the connection type, it is possible to establish the fastening by means of tooth, snap or any other connection technique.

In the injector arrangement developed by the present invention, a piston tip seal (102) having a concave structure is introduced at the tip of the said modular piston (103) with movable structure. The concavity of the said piston tip seal (102) prevents the stem cells to leak in between the seal and the outer wall of the injector during the centrifuge process in order to prevent volume losses.