Title:
TRANSCRIPTIONAL REGULATORY ELEMENT AND ITS USE IN ENHANCING THE EXPRESSION OF HETEROLOGOUS PROTEIN
Document Type and Number:
WIPO Patent Application WO/2020/034986
Kind Code:
A1
Abstract:
Provided is a polynucleotide, the polynucleotide can be used as a WXRE transcriptional regulatory element used to increase the protein expression level of a protein expression system. A protein expression vector or a protein expression system comprising the above-mentioned WXRE transcriptional regulatory element as well as the use thereof are also provided. The use of the WXRE transcriptional regulatory element can increase the expression level of a heterologous protein greatly with its biological activity unchanged.
Inventors:
ZHANG ZHENG (CN)
LI XIAOLU (CN)
ZHANG YUCHEN (CN)
LI YARONG (CN)
DONG HUIFANG (CN)
CAI JILL (CN)
ZHOU WEICHANG (CN)
CHEN CHRIS (CN)
MU LILI (CN)
TANG CHUANLONG (CN)
LI XIAOLU (CN)
ZHANG YUCHEN (CN)
LI YARONG (CN)
DONG HUIFANG (CN)
CAI JILL (CN)
ZHOU WEICHANG (CN)
CHEN CHRIS (CN)
MU LILI (CN)
TANG CHUANLONG (CN)
Application Number:
PCT/CN2019/100549
Publication Date:
February 20, 2020
Filing Date:
August 14, 2019
Export Citation:
Assignee:
WUXI BIOLOGICS SHANGHAI CO LTD (CN)
International Classes:
C12N5/10; C12N15/113; C12N15/64; C12N15/85; C12P21/08
Domestic Patent References:
| WO2017184831A1 | 2017-10-26 |
Foreign References:
| CN102459586A | 2012-05-16 | |||
| CN106987559A | 2017-07-28 | |||
| US20110076760A1 | 2011-03-31 | |||
| US20160159926A1 | 2016-06-09 |
Other References:
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold3125, whole genome shotgun sequence", XP055689012, retrieved from NCBI Database accession no. NW_003614865
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffodd37, whole genome shotgun sequence", XP055689015, retrieved from NCBI Database accession no. NW_003615153
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 C41403100, whole genome shotgun sequence", XP055689021, retrieved from NCBI Database accession no. NW_003630025
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold27679, whole genome shotgun sequence", XP055689028, retrieved from NCBI Database accession no. NW_003619752.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold.24313, whole genome shotgun sequence", XP055689037, retrieved from NCBI Database accession no. NW_003646628.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold1689, whole genome shotgun sequence", XP055689061, retrieved from NCBI Database accession no. NW_003613694.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffodd928, whole genome shotgun sequence", retrieved from NCBI Database accession no. NW_003614323.1
JAHANDAR, H. ET AL.: "Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells", IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH, vol. 14, no. 1, 31 December 2015 (2015-12-31), pages 177 - 187, XP055689008
"Current Protocols in Molecular Biology", 1989, JOHN WILEY AND SONS, pages: 1 - 6
"Molecular Cloning: A Laboratory Manual", COLD SPRING HARBOR LABORATORY
See also references of EP 3837368A4
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffodd37, whole genome shotgun sequence", XP055689015, retrieved from NCBI Database accession no. NW_003615153
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 C41403100, whole genome shotgun sequence", XP055689021, retrieved from NCBI Database accession no. NW_003630025
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold27679, whole genome shotgun sequence", XP055689028, retrieved from NCBI Database accession no. NW_003619752.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold.24313, whole genome shotgun sequence", XP055689037, retrieved from NCBI Database accession no. NW_003646628.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffold1689, whole genome shotgun sequence", XP055689061, retrieved from NCBI Database accession no. NW_003613694.1
DATABASE Nucleotide 27 May 2016 (2016-05-27), "Cricetulus griseus unplaced genomic scaffold, CriGri_1.0 scaffodd928, whole genome shotgun sequence", retrieved from NCBI Database accession no. NW_003614323.1
JAHANDAR, H. ET AL.: "Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells", IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH, vol. 14, no. 1, 31 December 2015 (2015-12-31), pages 177 - 187, XP055689008
"Current Protocols in Molecular Biology", 1989, JOHN WILEY AND SONS, pages: 1 - 6
"Molecular Cloning: A Laboratory Manual", COLD SPRING HARBOR LABORATORY
See also references of EP 3837368A4
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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