TWO-PHOTON FLUORESCENCE IMAGING METHOD AND SYSTEM, AND IMAGE PROCESSING DEVICE

WIPO Patent Application WO/2020/192235

A1

Provided are a two-photon fluorescence imaging method and system, and an image processing device, which are applicable to the technical field of two-photon fluorescence. The method comprises: performing wavefront shaping on two incident pulsed light beams in sequence to obtain two light beams, light fields of which at a focal point of an objective lens are gradient light fields, and making light intensity distributions of the gradient light fields of the two light beams be opposite; and then scanning a sample to be tested through the two light beams sequentially to obtain two images, and performing three-dimensional data reconstruction according to the two images to obtain a three-dimensional depth image of the sample to be tested. According to the method, the imaging speed is high, the resolution is high, and the algorithm is simple, and the present application is easily implemented; furthermore, since the imaging speed is high, light damage and a photobleaching effect on the sample to be tested are effectively reduced, and the present application is especially applicable to performing three-dimensional imaging on embryonic development samples and neural activity samples.

GAO YUFENG (CN)
ZHENG WEI (CN)

PCT/CN2019/130895

October 01, 2020

December 31, 2019

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SHENZHEN INST OF ADV TECH CAS (CN)

G01N21/64; G02B21/00

WO2008025565A1

2008-03-06

CN110006861A	2019-07-12
CN102944540A	2013-02-27
CN101915752A	2010-12-15
CN104407436A	2015-03-11
CN107091825A	2017-08-25
CN105784662A	2016-07-20

SHENZHEN ZHONGYI UNION INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)

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