TWO-PHOTON MICROSCOPIC IMAGING SYSTEM HAVING LOW LIGHT DAMAGE

Title:

TWO-PHOTON MICROSCOPIC IMAGING SYSTEM HAVING LOW LIGHT DAMAGE

Document Type and Number:

WIPO Patent Application WO/2023/273194

Kind Code:

A1

Abstract:

A two-photon microscopic imaging system having low light damage. An excitation light emitted by a femtosecond pulse laser (1) is modulated by a photoelectric modulator (2), and can then realize, by means of a first movable reflection mirror (4) and a second movable reflection mirror (11), switching between a first scanning module (8) and a second scanning module (10). The first scanning module (8) can perform resting-state imaging of biological living tissue by means of raster scanning, and the second scanning module (10) can realize video-level fast imaging, such that fast-changing neural activity in the biological living tissue can be observed. A photoelectric modulator is controlled in real time during an imaging process, such that gating excitation at a specific pixel point, such as a target cell, can be realized, so as to reduce ineffective radiation of laser light on an object under test, thereby decreasing light damage. The two-photon imaging system can reduce the thermal damage effect of traditional two-photon excitation, and is suitable for long-term living body imaging.

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Inventors:

LI BAOQIANG (CN)
WANG YANG (CN)

Application Number:

PCT/CN2021/137975

Publication Date:

January 05, 2023

Filing Date:

December 14, 2021

Export Citation:

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Assignee:

SHENZHEN INST ADV TECH (CN)

International Classes:

G01N21/64; G02B21/00; G02F2/00

Foreign References:

CN106290284A	2017-01-04
CN103735249A	2014-04-23
CN110941100A	2020-03-31
CN110132919A	2019-08-16
US20160003741A1	2016-01-07

Attorney, Agent or Firm:

BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)

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