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Patent Searching and Data


Title:
USE OF AMINO ACIDS TO ENHANCE SIGNAL IN MASS SPECTRAL ANALYSES
Document Type and Number:
WIPO Patent Application WO/2021/142137
Kind Code:
A1
Abstract:
A method of enhancing a mass spectral signal is disclosed. The method can include contacting a sample to a separation column under conditions that permit sample components to bind to the substrate; applying a first mobile gradient to the separation column, wherein the first mobile phase gradient comprises trifluoroacetic acid (TFA) and a small molecule additive (e.g., an amino acid) or formic acid (FA) and a small molecule additive (e.g., an amino acid); applying a second mobile gradient to the separation column, wherein the second mobile phase gradient comprises TFA in acetonitrile (ACN) and a small molecule additive (e.g., an amino acid) or formic acid (FA) in ACN and a small molecule additive (e.g., an amino acid); and performing mass spectrometric analysis on eluted sample components.

Inventors:
MAO YUAN (US)
KLEINBERG ANDREW (US)
ZHAO YUNLONG (US)
GUO LILI (US)
Application Number:
PCT/US2021/012523
Publication Date:
July 15, 2021
Filing Date:
January 07, 2021
Export Citation:
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Assignee:
REGENERON PHARMA (US)
International Classes:
G01N33/68; G01N30/02
Foreign References:
US20100331527A12010-12-30
US6596541B22003-07-22
Other References:
MAO YUAN ET AL: "Simple Addition of Glycine in Trifluoroacetic Acid-Containing Mobile Phases Enhances the Sensitivity of Electrospray Ionization Mass Spectrometry for Biopharmaceutical Characterization", ANALYTICAL CHEMISTRY, vol. 92, no. 13, 28 May 2020 (2020-05-28), US, pages 8691 - 8696, XP055796110, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.0c01319
ZHAO YUNLONG ET AL: "Glycine additive facilitates site-specific glycosylation profiling of biopharmaceuticals by ion-pairing hydrophilic interaction chromatography mass spectrometry", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 413, no. 5, 26 November 2020 (2020-11-26), pages 1267 - 1277, XP037373229, ISSN: 1618-2642, DOI: 10.1007/S00216-020-03089-3
GARCIA ET AL: "The effect of the mobile phase additives on sensitivity in the analysis of peptides and proteins by high-performance liquid chromatography-electrospray mass spectrometry", JOURNAL OF CHROMATOGRAPHY B, ELSEVIER, AMSTERDAM, NL, vol. 825, no. 2, 25 October 2005 (2005-10-25), pages 111 - 123, XP027627025, ISSN: 1570-0232, [retrieved on 20051025]
SHOU W Z ET AL: "Simple means to alleviate sensitivity loss by trifluoroacetic acid (TFA) mobile phases in the hydrophilic interaction chromatography-electrospray tandem mass spectrometric (HILIC-ESI/MS/MS) bioanalysis of basic compounds", JOURNAL OF CHROMATOGRAPHY B, ELSEVIER, AMSTERDAM, NL, vol. 825, no. 2, 25 October 2005 (2005-10-25), pages 186 - 192, XP027627033, ISSN: 1570-0232, [retrieved on 20051025]
LEFRANC ET AL.: "IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains", DEV. COMP. IMMUNOL., vol. 27, no. 1, 2003, pages 55 - 77, XP055585227, DOI: 10.1016/S0145-305X(02)00039-3
M. POTTER: "Structural correlates of immunoglobulin diversity", SURV. IMMUNOL. RES., vol. 2, no. 1, 1983, pages 27 - 42
WARD ET AL., NATURE, vol. 241, 1989, pages 544 - 546
POLJAK, STRUCTURE, vol. 2, 1994, pages 1121 - 1123
FRANK KJELDSEN ET AL., ANAL. CHEM., vol. 75, 2003, pages 2355 - 2361
ALPERT ET AL., J. CHROMATOGR. A, vol. 676, 1994, pages 191 - 202
WUHRER ET AL., ANAL. CHEM., vol. 76, 2004, pages 833 - 838
WUHRER ET AL., INTERNAT. J. MASS. SPEC., vol. 232, 2004, pages 51 - 57
GENOME TECHNOLOGY, pages 52
Attorney, Agent or Firm:
TERMES, Lance A. (US)
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