Title:
USE OF MALONYLTRANSFERASE GENE
Document Type and Number:
WIPO Patent Application WO/2019/009257
Kind Code:
A1
Abstract:
The problem to be solved by the present invention is to provide a method for producing a flavone having a malonyl group added to glucose located at position-7 therein using a polynucleotide encoding a protein having an activity to transfer a malonyl group to position-6 in glucose located at position-7 in flavone 7-glucoside, and to produce a plant variety which contains a flavone having a malonyl group added to glucose located at position-7 as a co-pigment and therefore can have an altered flower color, preferably a bluer flower color than the colors of the existing varieties. A polynucleotide is used, which is selected from the group consisting of: (a) a polynucleotide which comprises the nucleotide sequence represented by SEQ ID NO: 1; (b) a polynucleotide which can hybridize with a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein having an activity to transfer a malonyl group to position-6 in glucose located at position-7 in flavone 7-glucoside; (c) a polynucleotide which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2; and (d) a polynucleotide which encodes a protein comprising an amino acid sequence produced by deleting, substituting, inserting and/or adding one or several amino acid residues in the amino acid sequence represented by SEQ ID NO: 2 and having an activity to transfer a malonyl group to position-6 in glucose located at position-7 in flavone 7-glucoside; and others.
Inventors:
OKITSU NAOKO (JP)
NAKAMURA NORIKO (JP)
NAKAMURA NORIKO (JP)
Application Number:
PCT/JP2018/025101
Publication Date:
January 10, 2019
Filing Date:
July 02, 2018
Export Citation:
Assignee:
SUNTORY HOLDINGS LTD (JP)
International Classes:
C12N15/54; A01H5/00; A01N3/00; C12N5/10; C07D311/30
Domestic Patent References:
| WO2017002945A1 | 2017-01-05 |
Other References:
LIN, L. C. ET AL.: "Isolation of Luteolin and Luteolin-7-O-glucoside from Dendranthema morifolium Ramat Tzvel and Their Pharmacokinetics in Rats", J. AGRIC. FOOD CHEM., vol. 63, 2015, pages 7700 - 7706, XP009510027
UNNO, H. ET AL.: "Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the features of BAHD Enzyme Catalysis", J. BIOL. CHEM., vol. 282, no. 21, 2007, pages 15812 - 15822, XP055565321
NAKAYAMA, TOORU ET AL.: "X-ray crystal structure analysis of chrysanthemum petal- derived anthocyanin malonyl group transferase homologue Dm3MaT3", LECTURE ABSTRACTS OF CONFERENCE AND SYMPOSIUM OF THE JAPANESE SOCIETY OF PLANT CELL AND MOLECULAR BIOLOGY, vol. 25, 2007, pages 122
MANJASETTY, B. A. ET AL.: "Structural basis for modification of flavonol and naphthol glucoconjugates by Nicotiana tabacum malonyltransferase (NtMaTl)", PLANTA, vol. 236, 2012, pages 781 - 793, XP035103033
NODA, NAONOBU ET AL.: "Creation of blue flower with blue color and blue chrysanthemum by biotechnology", HORITICULTURAL RESEARCH, vol. 17, no. 1, 24 March 2018 (2018-03-24), pages 234
UNNO, H. ET AL.: "Structural and Mutational Studies of Anthocyanin Malonyltransferases Establish the features of BAHD Enzyme Catalysis", J. BIOL. CHEM., vol. 282, no. 21, 2007, pages 15812 - 15822, XP055565321
NAKAYAMA, TOORU ET AL.: "X-ray crystal structure analysis of chrysanthemum petal- derived anthocyanin malonyl group transferase homologue Dm3MaT3", LECTURE ABSTRACTS OF CONFERENCE AND SYMPOSIUM OF THE JAPANESE SOCIETY OF PLANT CELL AND MOLECULAR BIOLOGY, vol. 25, 2007, pages 122
MANJASETTY, B. A. ET AL.: "Structural basis for modification of flavonol and naphthol glucoconjugates by Nicotiana tabacum malonyltransferase (NtMaTl)", PLANTA, vol. 236, 2012, pages 781 - 793, XP035103033
NODA, NAONOBU ET AL.: "Creation of blue flower with blue color and blue chrysanthemum by biotechnology", HORITICULTURAL RESEARCH, vol. 17, no. 1, 24 March 2018 (2018-03-24), pages 234
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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