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Patent Searching and Data


Title:
VISUALIZED SCREENING METHOD FOR MULTIPLE-TARGETED EDITING OF RECOMBINANT ASPERGILLUS STRAINS
Document Type and Number:
WIPO Patent Application WO/2021/032180
Kind Code:
A1
Abstract:
Provided is a visualized screening method for multiple-targeted editing of recombinant aspergillus strains. The method utilizes CRISPR-Cas9 to simultaneously cleave genes related to spore color changes and target genes in Aspergillus so as to visualize target gene editing, so that Aspergillus multi-gene editing strains may be quickly and efficiently screened by means of spore phenotypes. By means of different combinations of visualized genes and genes without phenotypic changes, rapid screening of multi-gene editing strains and simultaneous screening of multiple visualized genes can be implemented, which may reduce the use of resistance genes in industrial strains.

Inventors:
LIU SONG (CN)
LI CEN (CN)
DU GUOCHENG (CN)
CHEN JIAN (CN)
ZHOU JINGWEN (CN)
Application Number:
PCT/CN2020/110404
Publication Date:
February 25, 2021
Filing Date:
August 21, 2020
Export Citation:
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Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/15; C12N15/113; C12N15/80; C12N15/90; C12R1/685
Foreign References:
CN110438018A2019-11-12
Other References:
SONG LETIAN, OUEDRAOGO JEAN-PAUL, KOLBUSZ MAGDALENA, NGUYEN THI TRUC MINH, TSANG ADRIAN: "Efficient Genome Editing Using tRNA Promoter-Driven CRISPR/Cas9 Grna in Aspergillus Niger", PLOS ONE, vol. 13, no. 8, e0202868, 24 August 2019 (2019-08-24), pages 1 - 17, XP055782412, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0202868
DATABASE Nucleotide 14 March 2015 (2015-03-14), ANONYMOUS: "Aspergillus Niger Contig An09c0170, Genomic Contig", XP055782419, retrieved from Genbank Database accession no. AM270206
HERMAN J PEL , JOHANNES H DE WINDE, DAVID B ARCHER, PAUL S DYER, GERALD HOFMANN, PETER J SCHAAP, GEOFFREY TURNER, RONALD P DE VRIE: "Genome Sequencing and Analysis of the Versatile Cell Factory Aspergillus Niger CBS 513.88", NATURE BIOTECHNOLOGY, vol. 25, no. 2, 1 February 2007 (2007-02-01), pages 221 - 231, XP002684207, ISSN: 1087-0156, DOI: 10.1038/nbt1282
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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