WATER-SOLUBLE POLYNORBORNENE DYES HAVING PENDANT

CHROMOPHORES

INTRODUCTION

Fluorescent dyes are compounds which, when irradiated with light of a wavelength which they absorb, emit light of a (usually) different wavelength. Fluorescent dyes find use in a variety of applications in biochemistry, biology and medicine, e.g. in diagnostic kits, in microscopy or in drug screening. Fluorescent dyes are characterized by a number of parameters allowing a user to select a suitable dye depending on the desired purpose.

Parameters of interest include the excitation wavelength maximum, the emission wavelength maximum, the Stokes shift, the extinction coefficient, the fluorescence quantum yield and the fluorescence lifetime. Dyes may be selected according to the application of interest in order to, e.g., allow penetration of exciting radiation into biological samples, to minimize background fluorescence and/or to achieve a high signal-to-noise ratio.

Flow cytometers use fluorophores to analyze complex and heterogeneous cell populations. The number of cell parameters that a flow cytometer can analyze is often limited by the number of fluorophores that can be simultaneously measured. Current commercial flow cytometers can usually measure 15 parameters, of which 13 are fluorescent parameters and 2 are physical (from scattered excitation light from the cell surface). Fluorophores that are used for conventional flow cytometry include small molecule organic dyes, fluorescent proteins, and semiconducting nanocrystals.

SUMMARY

Water soluble light harvesting multichromophores having a plurality of pendant chromophore groups are provided. Water soluble light harvesting multichromophores according to certain embodiments are polymers that include a polymeric backbone having norbornene repeat units, a pendant water solubilizing group component and a pendant chromophore component. In embodiments, each water solubilizing group and each chromophore group are linked to a norbornene repeat unit of the polymeric backbone. Polymeric tandem dyes based on the subject multichromophores are also provided that further include an acceptor fluorophore linked to a norbornene repeat unit of the polymeric backbone and configured in energy-receiving proximity to at least one pendant donor chromophore group of the light harvesting

multichromophore. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

DEFINITIONS

Before describing exemplary embodiments in greater detail, the following definitions are set forth to illustrate and define the meaning and scope of the terms used in the description.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Still, certain terms are defined below for the sake of clarity and ease of reference.

It must be noted that as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. For example, the term "a dye" refers to one or more dyes, i.e., a single dye and multiple dyes. It is further noted that the claims can be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as "solely," "only" and the like in connection with the recitation of claim elements, or use of a "negative" limitation.

As used herein, the terms "chemoselective functional group" and "chemoselective tag" are used interchangeably and refer to a functional group that can selectively react with another compatible functional group to form a covalent bond, in some cases, after optional activation of one of the functional groups. Chemoselective functional groups of interest include, but are not limited to, thiols and maleimide or iodoacetamide, amines and carboxylic acids or active esters thereof, as well as groups that can react with one another via Click chemistry, e.g., azide and alkyne groups (e.g., cyclooctyne groups), tetrazine, transcyclooctene, dienes and dienophiles, and azide, sulfur(VI) fluoride exchange chemistry (SuFEX), sulfonyl fluoride, as well as hydroxyl, hydrazido, hydrazino, aldehyde, ketone, azido, alkyne, phosphine, epoxide, and the like.

As used herein, the term "sample" relates to a material or mixture of materials, in some cases in liquid form, containing one or more analytes of interest. In some embodiments, the term as used in its broadest sense, refers to any plant, animal or bacterial material containing cells or producing cellular metabolites, such as, for example, tissue or fluid isolated from an individual (including without limitation plasma, serum, cerebrospinal fluid, lymph, tears, saliva and tissue sections) or from in vitro cell culture constituents, as well as samples from the environment. The term "sample" may also refer to a "biological sample". As used herein, the term "a biological sample" refers to a whole organism or a subset of its tissues, cells or component parts (e.g. body fluids, including, but not limited to, blood, mucus, lymphatic fluid, synovial fluid, cerebrospinal fluid, saliva, amniotic fluid, amniotic cord blood, urine, vaginal fluid and semen). A "biological sample" can also refer to a homogenate, lysate or extract prepared from a whole organism or a subset of its tissues, cells or component parts, or a fraction or portion thereof, including but not limited to, plasma, serum, spinal fluid, lymph fluid, the external sections of the skin, respiratory, intestinal, and genitourinary tracts, tears, saliva, milk, blood cells, tumors and organs. In certain embodiments, the sample has been removed from an animal or plant. Biological samples may include cells. The term "cells" is used in its

conventional sense to refer to the basic structural unit of living organisms, both eukaryotic and prokaryotic, having at least a nucleus and a cell membrane. In certain embodiments, cells include prokaryotic cells, such as from bacteria. In other embodiments, cells include eukaryotic cells, such as cells obtained from biological samples from animals, plants or fungi.

The terms "support bound" and "linked to a support" are used interchangeably and refer to a moiety (e.g., a specific binding member) that is linked covalently or non-covalently to a support of interest. Covalent linking may involve the chemical reaction of two compatible functional groups (e.g., two chemoselective functional groups, an electrophile and a nucleophile, etc.) to form a covalent bond between the two moieties of interest (e.g. a support and a specific binding member). In some cases, non-covalent linking may involve specific binding between two moieties of interest (e.g., two affinity moieties such as a hapten and an antibody or a biotin moiety and a streptavidin, etc.). In certain cases, non-covalent linking may involve absorption to a substrate.

The term "polypeptide" refers to a polymeric form of amino acids of any length, including peptides that range from 2-50 amino acids in length and polypeptides that are greater than 50 amino acids in length. The terms "polypeptide" and "protein" are used interchangeably herein. The term "polypeptide" includes polymers of coded and non-coded amino acids, chemically or biochemically modified or derivatized amino acids, and polypeptides having modified peptide backbones in which the conventional backbone has been replaced with non-naturally occurring or synthetic backbones. A polypeptide may be of any convenient length, e.g., 2 or more amino acids, such as 4 or more amino acids, 10 or more amino acids, 20 or more amino acids, 50 or more amino acids, 100 or more amino acids, 300 or more amino acids, such as up to 500 or 1000 or more amino acids. "Peptides" may be 2 or more amino acids, such as 4 or more amino acids, 10 or more amino acids, 20 or more amino acids, such as up to 50 amino acids. In some embodiments, peptides are between 5 and 30 amino acids in length. As used herein the term "isolated," refers to an moiety of interest that is at least 60% free, at least 75% free, at least 90% free, at least 95% free, at least 98% free, and even at least 99% free from other components with which the moiety is associated with prior to purification.

A "plurality" contains at least 2 members. In certain cases, a plurality may have 5 or more, such as 6 or more, 7 or more, 8 or more, 9 or more, 10 or more, 20 or more, 30 or more, 40 or more, 50 or more, 60 or more, 70 or more, 80 or more, 90 or more, 100 or more, 300 or more, 1000 or more, 3000 or more, 10,000 or more, 100,000 or more members.

Numeric ranges are inclusive of the numbers defining the range.

The term "specific binding" refers to the ability of a capture agent (or a first member of a specific binding pair) to preferentially bind to a particular analyte (or a second member of a specific binding pair) that is present, e.g., in a homogeneous mixture of different analytes. In some instances, a specific binding interaction will discriminate between desirable and undesirable analytes in a sample with a specificity of 10-fold or more for a desirable analyte over an undesirable analytes, such as 100-fold or more, or 1000-fold or more. In some cases, the affinity between a capture agent and analyte when they are specifically bound in a capture agent/analyte complex is at least 10 ^-8 M, at least 10 ^-9 M, such as up to 10 ^_10 M.

"Affinity" refers to the strength of binding, increased binding affinity being correlated with a lower Kd.

The methods described herein include multiple steps. Each step may be performed after a predetermined amount of time has elapsed between steps, as desired. As such, the time between performing each step may be 1 second or more, 10 seconds or more, 30 seconds or more, 60 seconds or more, 5 minutes or more, 10 minutes or more, 60 minutes or more and including 5 hours or more. In certain embodiments, each subsequent step is performed immediately after completion of the previous step. In other embodiments, a step may be performed after an incubation or waiting time after completion of the previous step, e.g., a few minutes to an overnight waiting time.

As used herein, the terms "evaluating", "determining," "measuring," and "assessing," and "assaying" are used interchangeably and include both quantitative and qualitative

determinations.

The term "separating", as used herein, refers to physical separation of two elements (e.g., by size or affinity, etc.) as well as degradation of one element, leaving the other intact.

The term "linker" or "linkage" refers to a linking moiety that connects two groups and has a backbone of 100 atoms or less in length. A linker or linkage may be a covalent bond that connects two groups or a chain of between 1 and 100 atoms in length, for example a chain of 1 , 2, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20 or more carbon atoms in length, where the linker may be linear, branched, cyclic or a single atom. In some cases, the linker is a branching linker that refers to a linking moiety that connects three or more groups. In certain cases, one, two, three, four or five or more carbon atoms of a linker backbone may be optionally substituted with a sulfur, nitrogen or oxygen heteroatom. In some cases, the linker backbone includes a linking functional group, such as an ether, thioether, amino, amide, sulfonamide, carbamate, thiocarbamate, urea, thiourea, ester, thioester or imine. The bonds between backbone atoms may be saturated or unsaturated, and in some cases not more than one, two, or three unsaturated bonds are present in a linker backbone. The linker may include one or more substituent groups, for example with an alkyl, aryl or alkenyl group. A linker may include, without limitations, polyethylene glycol; ethers, thioethers, tertiary amines, alkyls, which may be straight or branched, e.g., methyl, ethyl, n-propyl, 1 -methylethyl (iso-propyl), n-butyl, n-pentyl,

1 ,1 -dimethylethyl (t-butyl), and the like. The linker backbone may include a cyclic group, for example, an aryl, a heterocycle or a cycloalkyl group, where 2 or more atoms, e.g., 2, 3 or 4 atoms, of the cyclic group are included in the backbone. A linker may be cleavable or non- cleavable.

The terms "polyethylene oxide", "PEO", "polyethylene glycol" and "PEG" are used interchangeably and refer to a polymeric group including a chain described by the formula - (CH ₂ -CH ₂ -0-) _n - or a derivative thereof. In some embodiments, "n" is 5000 or less, such as 1000 or less, 500 or less, 200 or less, 100 or less, 50 or less, 40 or less, 30 or less, 20 or less,

15 or less, such as 3 to 15, or 10 to 15. It is understood that the PEG polymeric group may be of any convenient length and may include a variety of terminal groups and/or further substituent groups, including but not limited to, alkyl, aryl, hydroxyl, amino, acyl, acyloxy, and amido terminal and/or substituent groups. PEG groups that may be adapted for use in the subject multichromophores include those PEGs described by S. Zalipsky in "Functionalized

polyethylene glycol) for preparation of biologically relevant conjugates", Bioconjugate

Chemistry 1995, 6 (2), 150-165; and by Zhu et al in "Water-Soluble Conjugated Polymers for Imaging, Diagnosis, and Therapy", Chem. Rev., 2012, 1 12 (8), pp 4687-4735 .

The term "alkyl" by itself or as part of another substituent refers to a saturated branched or straight-chain monovalent hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of a parent alkane. Alkyl groups of interest include, but are not limited to, methyl; ethyl, propyls such as propan-1 -yl or propan-2-yl; and butyls such as butan-1 -yl, butan-2-yl, 2-methyl-propan-1 -yl or 2-methyl-propan-2-yl. In some embodiments, an alkyl group includes from 1 to 20 carbon atoms. In some embodiments, an alkyl group includes from 1 to 10 carbon atoms. In certain embodiments, a lower alkyl group includes from 1 to 6 carbon atoms, such as from 1 to 4 carbon atoms. This term includes, by way of example, linear and branched hydrocarbyl groups such as methyl (CH ₃ -), ethyl (CH ₃ CH ₂ -), n-propyl

(CH ₃ CH ₂ CH ₂ -), isopropyl ((CH ₃ ) ₂ CH-), n-butyl (CH ₃ CH ₂ CH ₂ CH ₂ -), isobutyl ((CH ₃ ) ₂ CHCH ₂ -), sec- butyl ((CH ₃ )(CH ₃ CH ₂ )CH-), t-butyl ((CH ₃ ) ₃ C-), n-pentyl (CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ -), and neopentyl ((CH ₃ ) ₃ CCH ₂ -).

The term "substituted alkyl" refers to an alkyl group as defined herein wherein one or more carbon atoms in the alkyl chain have been optionally replaced with a heteroatom such as -0-, -N-, -S-, -S(O) _n - (where n is 0 to 2), -NR- (where R is hydrogen or alkyl) and having from 1 to 5 substituents selected from the group consisting of alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, oxo, thioketo, carboxyl, carboxylalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, heterocyclooxy, hydroxyamino, alkoxyamino, nitro, -SO-alkyl, -SO-aryl, -SO-heteroaryl, -SO ₂ -alkyl, -SO ₂ - aryl, -SO ₂ -heteroaryl, and -NR ^a R ^b , wherein R ^’ and R ^” may be the same or different and are chosen from hydrogen, optionally substituted alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heteroaryl and heterocyclic.

"Alkoxy" refers to the group -O-alkyl, wherein alkyl is as defined herein. Alkoxy includes, by way of example, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, t-butoxy, sec- butoxy, n-pentoxy, and the like. The term "alkoxy" also refers to the groups alkenyl-O-, cycloalkyl-O-, cycloalkenyl-O-, and alkynyl-O-, where alkenyl, cycloalkyl, cycloalkenyl, and alkynyl are as defined herein.

The term "substituted alkoxy" refers to the groups substituted alkyl-O-, substituted alkenyl-O-, substituted cycloalkyl-O-, substituted cycloalkenyl-O-, and substituted alkynyl-O- where substituted alkyl, substituted alkenyl, substituted cycloalkyl, substituted cycloalkenyl and substituted alkynyl are as defined herein.

"Alkynyl" refers to straight or branched monovalent hydrocarbyl groups having from 2 to 6 carbon atoms and preferably 2 to 3 carbon atoms and having at least 1 and preferably from 1 to 2 sites of triple bond unsaturation. Examples of such alkynyl groups include acetylenyl (-CºCH), and propargyl (-CH ₂ CºCH).

The term "substituted alkynyl" refers to an alkynyl group as defined herein having from 1 to 5 substituents, or from 1 to 3 substituents, selected from alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, acyl, acylamino, acyloxy, amino, substituted amino, aminoacyl, aminoacyloxy, oxyaminoacyl, azido, cyano, halogen, hydroxyl, oxo, thioketo, carboxyl, carboxylalkyl, thioaryloxy, thioheteroaryloxy, thioheterocyclooxy, thiol, thioalkoxy, substituted thioalkoxy, aryl, aryloxy, heteroaryl,

heteroaryloxy, heterocyclyl, heterocyclooxy, hydroxyamino, alkoxyamino, nitro, -SO-alkyl, -SO- substituted alkyl, -SO-aryl, -SO-heteroaryl, -SO ₂ -alkyl, -SO ₂ -substituted alkyl, -SO ₂ -aryl, and - SO ₂ -heteroaryl.

"Amino" refers to the group -NH ₂ . The term "substituted amino" refers to the group -NRR where each R is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, alkenyl, substituted alkenyl, cycloalkenyl, substituted cycloalkenyl, alkynyl, substituted alkynyl, aryl, heteroaryl, and heterocyclyl provided that at least one R is not hydrogen.

"Aryl" by itself or as part of another substituent refers to a monovalent aromatic hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of an aromatic ring system. Aryl groups of interest include, but are not limited to, groups derived from aceanthrylene, acenaphthylene, acephenanthrylene, anthracene, azulene, benzene, chrysene, coronene, fluoranthene, fluorene, hexacene, hexaphene, hexalene, as-indacene, s- indacene, indane, indene, naphthalene, octacene, octaphene, octalene, ovalene, penta-2,4- diene, pentacene, pentalene, pentaphene, perylene, phenalene, phenanthrene, picene, pleiadene, pyrene, pyranthrene, rubicene, triphenylene, trinaphthalene and the like. In certain embodiments, an aryl group includes from 6 to 20 carbon atoms. In certain embodiments, an aryl group includes from 6 to 12 carbon atoms. Examples of an aryl group are phenyl and naphthyl.

"Substituted aryl", unless otherwise constrained by the definition for the aryl substituent, refers to an aryl group substituted with from 1 to 5 substituents, or from 1 to 3 substituents, selected from acyloxy, hydroxy, thiol, acyl, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl,

cycloalkenyl, substituted alkyl, substituted alkoxy, substituted alkenyl, substituted alkynyl, substituted cycloalkyl, substituted cycloalkenyl, amino, substituted amino, aminoacyl, acylamino, alkaryl, aryl, aryloxy, azido, carboxyl, carboxylalkyl, cyano, halogen, nitro, heteroaryl, heteroaryloxy, heterocyclyl, heterocyclooxy, aminoacyloxy, oxyacylamino, thioalkoxy, substituted thioalkoxy, thioaryloxy, thioheteroaryloxy, -SO-alkyl, -SO-substituted alkyl, -SO-aryl, -SO-heteroaryl, -SO ₂ -alkyl, -SO ₂ -substituted alkyl, -SO ₂ -aryl, -SO ₂ -heteroaryl and trihalomethyl.

"Heteroaryl" by itself or as part of another substituent, refers to a monovalent

heteroaromatic radical derived by the removal of one hydrogen atom from a single atom of a heteroaromatic ring system. Heteroaryl groups of interest include, but are not limited to, groups derived from acridine, arsindole, carbazole, b-carboline, chromane, chromene, cinnoline, furan, imidazole, indazole, indole, indoline, indolizine, isobenzofuran, isochromene, isoindole, isoindoline, isoquinoline, isothiazole, isoxazole, naphthyridine, oxadiazole, oxazole, perimidine, phenanthridine, phenanthroline, phenazine, phthalazine, pteridine, purine, pyran, pyrazine, pyrazole, pyridazine, pyridine, pyrimidine, pyrrole, pyrrolizine, quinazoline, quinoline, quinolizine, quinoxaline, tetrazole, thiadiazole, thiazole, triazole, benzotriazole, thiophene, triazole, xanthene, benzodioxole and the like. In certain embodiments, the heteroaryl group is from 5-20 membered heteroaryl. In certain embodiments, the heteroaryl group is from 5-10 membered heteroaryl. In certain embodiments, heteroaryl groups are those derived from thiophene, pyrrole, benzothiophene, benzofuran, indole, pyridine, quinoline, imidazole, oxazole and pyrazine.

"Heterocycle," "heterocyclic," "heterocycloalkyl," and "heterocyclyl" refer to a saturated or unsaturated group having a single ring or multiple condensed rings, including fused bridged and spiro ring systems, and having from 3 to 20 ring atoms, including 1 to 10 hetero atoms. These ring atoms are selected from the group consisting of nitrogen, sulfur, or oxygen, wherein, in fused ring systems, one or more of the rings can be cycloalkyl, aryl, or heteroaryl, provided that the point of attachment is through the non-aromatic ring. In certain embodiments, the nitrogen and/or sulfur atom(s) of the heterocyclic group are optionally oxidized to provide for the N-oxide, -S(O)-, or -SO ₂ - moieties.

Examples of heterocycles and heteroaryls include, but are not limited to, azetidine, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, dihydroindole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline,

phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine, indoline, phthalimide, 1 ,2,3,4- tetrahydroisoquinoline, 4,5,6,7-tetrahydrobenzo[b]thiophene, thiazole, thiazolidine, thiophene, benzo[b]thiophene, morpholinyl, thiomorpholinyl (also referred to as thiamorpholinyl), 1 ,1 - dioxothiomorpholinyl, piperidinyl, pyrrolidine, tetrahydrofuranyl, and the like.

"Substituted heteroaryl", unless otherwise constrained by the definition for the substituent, refers to an heteroaryl group substituted with from 1 to 5 substituents, or from 1 to 3 substituents, selected from acyloxy, hydroxy, thiol, acyl, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, substituted alkyl, substituted alkoxy, substituted alkenyl, substituted alkynyl, substituted cycloalkyl, substituted cycloalkenyl, amino, substituted amino, aminoacyl, acylamino, alkaryl, aryl, aryloxy, azido, carboxyl, carboxylalkyl, cyano, halogen, nitro, heteroaryl, heteroaryloxy, heterocyclyl, heterocyclooxy, aminoacyloxy, oxyacylamino, thioalkoxy, substituted thioalkoxy, thioaryloxy, thioheteroaryloxy, -SO-alkyl, -SO-substituted alkyl, -SO-aryl, -SO-heteroaryl, -SO ₂ -alkyl, -SO ₂ -substituted alkyl, -SO ₂ -aryl, -SO ₂ -heteroaryl and trihalomethyl.

The term "alkaryl" or "aralkyl" refers to the groups -alkylene-aryl and substituted alkylene-aryl where alkylene, substituted alkylene and aryl are defined herein.

"Alkylene" refers to divalent aliphatic hydrocarbyl groups preferably having from 1 to 6 and more preferably 1 to 3 carbon atoms that are either straight-chained or branched, and which are optionally interrupted with one or more groups selected from -O-, -NR ¹⁰ -, -NR ¹⁰ C(O)-, -C(O)NR ¹⁰ - and the like. This term includes, by way of example, methylene (-CH ₂ -), ethylene (-CH ₂ CH ₂ -), n-propylene (-CH ₂ CH ₂ CH ₂ -), iso-propylene (-CH ₂ CH(CH ₃ )-), (-C(CH ₃ ) ₂ CH ₂ CH ₂ -), (-C(CH ₃ ) ₂ CH ₂ C(O)-), (-C(CH ₃ ) ₂ CH ₂ C(O)NH-), (-CH(CH ₃ )CH ₂ -), and the like. "Substituted alkylene" refers to an alkylene group having from 1 to 3 hydrogens replaced with substituents as described for carbons in the definition of "substituted" below.

"Substituted" refers to a group in which one or more hydrogen atoms are independently replaced with the same or different substituent(s). Substituents of interest include, but are not limited to, alkylenedioxy (such as methylenedioxy), -M, -R ⁶⁰ , -O-, =O, -OR ⁶⁰ , -SR ⁶⁰ , -S-,

=S, -NR ⁶⁰ R ⁶¹ , =NR ⁶⁰ , -CF ₃ , -CN, -OCN, -SON, -NO, -NO ₂ ,

=N ₂ , -N ₃ , -S(O) ₂ O-, -S(O) ₂ OH, -S(O) ₂ R ⁶⁰ , -0S(O) ₂ O-, -OS(O) ₂ R ⁶⁰ , -P(O)(O ) ₂ , -P(O)(OR ⁶⁰ )(O ), -O P(O)(OR ⁶⁰ )(OR ⁶¹ ), -C(O)R ⁶⁰ , -C(S)R ⁶⁰ , -C(O)OR ⁶⁰ , -C(O)NR ⁶⁰ R ⁶¹ , -C(O)O-, -C(S)OR ⁶⁰ , -NR ⁶² C(O )NR ⁶⁰ R ⁶¹ , -NR ⁶² C(S)NR ⁶⁰ R ⁶¹ , -NR ⁶² C(NR ⁶³ )NR ⁶⁰ R ⁶¹ and -C(NR ⁶² )NR ⁶⁰ R ⁶¹ where M is halogen; R ⁶⁰ , R ⁶¹ , R ⁶² and R ⁶³ are independently hydrogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, substituted cycloheteroalkyl, aryl, substituted aryl, heteroaryl or substituted heteroaryl, or optionally R ⁶⁰ and R ⁶¹ together with the nitrogen atom to which they are bonded form a cycloheteroalkyl or substituted cycloheteroalkyl ring; and R ⁶⁴ and R ⁶⁵ are independently hydrogen, alkyl, substituted alkyl, aryl, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, substituted cycloheteroalkyl, aryl, substituted aryl, heteroaryl or substituted heteroaryl, or optionally R ⁶⁴ and R ⁶⁵ together with the nitrogen atom to which they are bonded form a cycloheteroalkyl or substituted cycloheteroalkyl ring. In certain embodiments, substituents include -M, -R ⁶⁰ , =O, -OR ⁶⁰ , -SR ⁶⁰ , -S , =S, -NR ⁶⁰ R ⁶¹ ,

=NR ⁶⁰ , -CF ₃ , -CN, -OCN, -SCN, -NO, -NO ₂ ,

=N ₂ , -N ₃ , -S(O) ₂ R ⁶⁰ , -OS(O) ₂ O-, -OS(O) ₂ R ⁶⁰ , -P(O)(O-) ₂ , -P(O)(OR ⁶⁰ )(O ), -OP(O)(OR ⁶⁰ )(OR ⁶¹ ), - C(O)R ⁶⁰ , -C(S)R ⁶⁰ , -C(O)OR ⁶⁰ , -C(O)NR ⁶⁰ R ⁶¹ ,-C(O)O-, -NR ⁶² C(O)NR ⁶⁰ R ⁶¹ . In certain embodiments, substituents include -M, -R ⁶⁰ ,

=O, -OR ⁶⁰ , -SR ⁶⁰ , -NR ⁶⁰ R ⁶¹ , -CF ₃ , -CN, -NO ₂ , -S(O) ₂ R ⁶⁰ , -P(O)(OR ⁶⁰ )(O ), -OP(O)(OR ⁶⁰ )(OR ⁶¹ ), - C(O)R ⁶⁰ , -C(O)OR ⁶⁰ , -C(O)NR ⁶⁰ R ⁶¹ ,-C(O)O\ In certain embodiments, substituents include -M, -R ⁶⁰ , =O, -OR ⁶⁰ , -SR ⁶⁰ , -NR ⁶⁰ R ⁶¹ , -CF ₃ , -CN, -NO ₂ , -S(O) ₂ R ⁶⁰ ,

-OP(O)(OR ⁶⁰ )(OR ⁶¹ ), -C(O)R ⁶⁰ , -C(O)OR ⁶⁰ ,-0(O)O , where R ⁶⁰ , R ⁶¹ and R ⁶² are as defined above. For example, a substituted group may bear a methylenedioxy substituent or one, two, or three substituents selected from a halogen atom, a (1 -4C)alkyl group and a (1 -4C)alkoxy group. When the group being substituted is an aryl or heteroaryl group, the substituent(s) (e.g., as described herein) may be referred to as "aryl substituent(s)".

"Sulfonylamino" refers to the group -NR ²¹ SO ₂ R ²² , wherein R ²¹ and R ²² independently are selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R ²¹ and R ²² are optionally joined together with the atoms bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic are as defined herein.

It is understood that in all substituted groups defined above, polymers arrived at by defining substituents with further substituents to themselves (e.g., substituted aryl having a substituted aryl group as a substituent which is itself substituted with a substituted aryl group, which is further substituted by a substituted aryl group, etc.) are not intended for inclusion herein. In such cases, the maximum number of such substitutions is three. For example, serial substitutions of substituted aryl groups specifically contemplated herein are limited to

substituted aryl-(substituted aryl)-substituted aryl.

Unless indicated otherwise, the nomenclature of substituents that are not explicitly defined herein are arrived at by naming the terminal portion of the functionality followed by the adjacent functionality toward the point of attachment. For example, the substituent

"arylalkyloxycarbonyl" refers to the group (aryl)-(alkyi)-0-C(O)-.

As to any of the groups disclosed herein which contain one or more substituents, it is understood, of course, that such groups do not contain any substitution or substitution patterns which are sterically impractical and/or synthetically non-feasible. In addition, the subject compounds include all stereochemical isomers arising from the substitution of these

compounds.

Other definitions of terms may appear throughout the specification. DETAILED DESCRIPTION

As summarized above, water soluble light harvesting multichromophores having a plurality of pendant chromophore groups are provided. Water soluble light harvesting multichromophores according to certain embodiments are polymers that include a polymeric backbone having norbornene repeat units, a pendant water solubilizing group component and a pendant chromophore component. In embodiments, each water solubilizing group and each chromophore group are linked to a norbornene repeat unit of the polymeric backbone.

Polymeric tandem dyes based on the subject multichromophores are also provided that further include an acceptor fluorophore linked to a norbornene repeat unit of the polymeric backbone and configured in energy-receiving proximity to at least one pendant donor chromophore group of the light harvesting multichromophore. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

Before the present invention is described in greater detail, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.

Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.

Certain ranges are presented herein with numerical values being preceded by the term "about." The term "about" is used herein to provide literal support for the exact number that it precedes, as well as a number that is near to or approximately the number that the term precedes. In determining whether a number is near to or approximately a specifically recited number, the near or approximating unrecited number may be a number which, in the context in which it is presented, provides the substantial equivalent of the specifically recited number.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, representative illustrative methods and materials are now described.

All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed.

It is noted that, as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as "solely," "only" and the like in connection with the recitation of claim elements, or use of a "negative" limitation.

As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present invention. Any recited method can be carried out in the order of events recited or in any other order which is logically possible.

While the apparatus and method has or will be described for the sake of grammatical fluidity with functional explanations, it is to be expressly understood that the claims, unless expressly formulated under 35 U.S.C. §1 12, are not to be construed as necessarily limited in any way by the construction of "means" or "steps" limitations, but are to be accorded the full scope of the meaning and equivalents of the definition provided by the claims under the judicial doctrine of equivalents, and in the case where the claims are expressly formulated under 35 U.S.C. §1 12 are to be accorded full statutory equivalents under 35 U.S.C. §1 12. In further describing the subject invention, polymers that include a polymeric backbone having norbornene repeat units, a pendant water solubilizing group component and a pendant chromophore component and related polymeric tandem dyes including an acceptor fluorophore are described first in greater detail. Next, labelled specific binding members which include the subject polymeric tandem dyes are described. Then, methods of interest in which the subject polymeric tandem dyes find use are reviewed. Systems and kits that may be used in practicing methods of the present disclosure are also described.

LIGHT HARVESTING MULTICHROMOPHORE POLYMERS HAVING A POLYNORBORNENE BACKBONE

As summarized above, the present disclosure includes light harvesting

multichromophore polymers that include a polymeric backbone having norbornene repeat units, a pendant water solubilizing group component and a pendant chromophore component, where each water solubilizing group and each chromophore group are linked (e.g., covalently bonded) to a norbornene repeat unit of the polymeric backbone. The term "pendant" is used herein to refer to a sidechain group that is connected to the polynorbornene backbone but which is not part of the backbone. In embodiments, the subject multichromophore polymers provide for the configuration of a plurality of light-absorbing chromophore groups in a compact area sufficient for efficient energy transfer between the chromophores and when present, to an acceptor fluorophore. Taken together, this configuration of pendant light-absorbing chromophore groups forms a light harvesting multichromophore having an absorption wavelength (e.g., as described herein) at which the optically active chromophore groups absorb light to form an excited state. As such, the light-absorbing chromophore groups are configured in energy-receiving proximity to each other and are capable of donating energy to an acceptor fluorophore when present.

The terms "light harvesting multichromophore" and "polymeric dye" are used

interchangeably and refer to a polymer of the present disclosure which includes a polymeric backbone having norbornene repeat units, a pendant water solubilizing group component and a pendant chromophore component where each water solubilizing group and each chromophore group are linked (e.g., covalently bonded) to a norbornene repeat unit of the polymeric backbone such that the polymer is capable of harvesting light with a particular absorption maximum wavelength and converting it to emitted light at a longer emission maximum wavelength.

Further pendant groups such as acceptor fluorophores, secondary donor chromophores, linkers and chemoselective tags capable of biomolecule conjugation can also be attached to the modular scaffold. In some cases, an acceptor fluorophore can be installed in conjunction with two types of donor chromophores (a primary and a secondary donor chromophore) to provide for a desired fluorescent emission from the acceptor fluorophore. The number and positioning of acceptor fluorophores relative to the configuration of pendant donor chromophores can be controlled.

A particular configuration of pendant groups can be determined and controlled by the arrangement of the norbornene repeat units to which the pendant groups are attached. In embodiments, the subject multichromophore polynorbornene polymers include a water solubilizing group component attached to the scaffold to provide a water soluble light harvesting multichromophore polymer. The polymer backbone is composed of norbornene repeat units which form a polymeric backbone to which the pendant groups (chromophore, water solubilizing group) are attached. The chromophore component and water solubilizing group component can be arranged in a variety of configurations to provide for a water soluble light harvesting multichromophore having desirable spectroscopic properties. The distances and arrangement between sites for covalent attachment of the pendant donor chromophores and the acceptor fluorophore (when present) can be controlled to provide for desirable energy transfer processes. This can lead to desirable high light harvesting and signal amplification properties.

In some embodiments, the configuration of pendant donor chromophore groups on the polynorbornene polymer backbone can exhibit, upon excitation with incident light, self quenching of fluorescence relative to an unquenched isolated donor chromophore group. By self-quenching is meant that 10% or more, such as 20% or more, 25% or more, 30% or more, 40% or more, or 50% or more of the fluorescence relative to unquenched isolated donor chromophore groups.

In some instances, the subject light harvesting multichromophore polymer includes a plurality of pendant donor chromophore groups each independently linked to a norbornene repeat unit of the polymeric backbone. The configuration of pendant groups can be installed during or after synthesis of the polynorbornene polymeric backbone. The incorporation of pendant groups can be with achieved with a random configuration, a block configuration, or in a sequence-specific manner via stepwise synthesis, depending on the particular method of synthesis utilized.

The term "unit" refers to a structural subunit of a polymer. The term unit is meant to include monomers, co-monomers, co-blocks, repeating units, and the like. A "repeating unit" or "repeat unit" is a subunit of a polymer that is defined by the minimum number of distinct structural features that are required for the unit to be considered monomeric, such that when the unit is repeated n times, the resulting structure describes the polymer or a block thereof. In some cases, the polymer may include two or more different repeating units, e.g., when the polymer is a multiblock polymer, a random arrangement of units or a defined sequence, each block may define a distinct repeating unit. It is understood that a variety of arrangements of repeating units or blocks are possible and that in the depicted formula of the subject multichromophores described herein any convenient linear arrangements of various lengths can be included within the structure of the overall polymer. It is understood that the polymer may also be represented by a formula in terms of mol% values of each unit in the polymer and that such formula may represent a variety of arrangements of repeat unit, such as random or multiblock polymer or a defined sequence of residues. In some cases, a repeating unit of the polymer includes a single monomer group. In certain instances, a repeating unit of the polymer includes two or more monomer groups, i.e., co-monomer groups, such as two, three, four or more co-monomer groups. The term "co-monomer" or "co-monomer group" refers to a structural unit of a polymer that may itself be part of a repeating unit of the polymer.

The subject light harvesting multichromophore polymers includes a linear polymeric backbone of norbornene repeat units. The polynorbornene polymeric backbone can include a random configuration of norbornene repeat units. The polynorbornene polymeric backbone can include a block or co-block configuration of norbornene repeat units. Alternatively, the polynorbornene polymeric backbone can include a particular defined sequence of norbornene repeat units. These configurations can be characterized by polymeric segments of norbornene repeat units (e.g., as described herein), which segments can themselves be repeated throughout the multichromophore polymer.

In some instances, the subject multichromophore polymers include a segment of the formula (NB-I):

wherein:

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ¹ R ² , R ³ and R ⁴ are each independently selected from hydrogen, a water solubilizing group and a chromophore, wherein at least one of R ¹ , R ² , R ³ and R ⁴ is a chromophore and at least one of R ¹ , R ² , R ³ and R ⁴ is a water solubilizing group;

Y ¹ and Y ² are each independently selected from CR ⁵ and O; R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore; and

n is an integer from 1 to 1000; and

m is an integer from 0 to 1000.

In some instances of formula (NB-I), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-I), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-I), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-I), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IA):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ⁹ , and R ¹⁰ are each independently a chromophore; and L ¹ -L ⁴ , Y ¹ , Y ² , n and m are as defined above.

In some instances of formula (NB-IA), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-IA), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-IA), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-IA), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IB):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ , L ² , L ⁴ , Y ¹ , Y ² , n and m are as defined above.

In some instances of formula (NB-IB), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-IB), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-IB), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-IB), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IC):

wherein:

R ⁷ is a water solubilizing group;

R ¹² is a chromophore; and L ¹ -L ² , Y ¹ , and n are as defined above.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-ID):

wherein:

R ⁷ , R ⁸ , and R ¹¹ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ -L ⁴ , Y ¹ , Y ² , n and m are as defined above.

In some instances of formula (NB-ID), n is 80 mol% or more, such as 85 mol% or more,

90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-ID), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-ID), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-ID), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IE):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ⁹ , and R ¹⁰ are each independently a chromophore; and L ¹ -L ⁴ , n and m are as defined above.

In some instances of formula (NB-IE), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-IE), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-IE), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-IE), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IF):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ , L ² , L ⁴ , n and m are as defined above.

In some instances of formula (NB-IF), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-IF), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-IF), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-IF), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IG):

wherein:

R ⁷ is a water solubilizing group;

R ¹² is a chromophore; and L ¹ -L ² , and n are as defined above.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IH): wherein:

R ⁷ , and R ⁸ , and R ¹¹ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ -L ⁴ , n and m are as defined above.

In some instances of formula (NB-IH), n is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more. In other instances of formula (NB-IH), n is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In some instances of formula (NB-IH), m is 20 mol% or less, such as 15 mol% or less, 10 mol% or less, 5 mol% or less, 4 mol% or less, 3 mol% or less, 2 mol% or less, 1 mol% or less. In other instances of (NB-IH), m is 80 mol% or more, such as 85 mol% or more, 90 mol% or more, 95 mol% or more, 96 mol% or more, 97 mol% or more, 98 mol% or more, or 99 mol% or more.

In some instances, the subject multichromophore polymers include a segment of the formula (NB-II):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ¹ , R ² , R ³ and R ⁴ are each independently selected from hydrogen, a water solubilizing group and a chromophore, wherein at least one of R ¹ , R ² , R ³ and R ⁴ is a chromophore and at least one of R ¹ , R ² , R ³ and R ⁴ is a water solubilizing group;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

m is an integer from 0 to 1000; and

r is an integer from 0 to 10.

In certain cases of formula (NB-II), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-II), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other cases of formula (NB-II), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-II), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-II), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-II), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-II), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIA):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ⁹ , and R ¹⁰ are each independently a chromophore; and L ¹ -L ⁴ , Y ¹ , Y ² , G ¹ , r, n and m are as defined above.

In certain cases of formula (NB-IIA), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIA), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other cases of formula (NB-IIA), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIA), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIA), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIA), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIA), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIB):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ , L ² , L ⁴ , Y ¹ , Y ² , G ¹ , r, n and m are as defined above.

In certain cases of formula (NB-IIB), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIB), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIB), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIB), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIB), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIB), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIB), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIC):

wherein:

R ⁷ is a water solubilizing group;

R ¹² is a chromophore; and L ¹ -L ² , Y ¹ , G ¹ , r, and n are as defined above.

In certain cases of formula (NB-IIC), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In other cases of formula (NB-IIC), r is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIC), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIC), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIC), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IID):

wherein:

R ⁷ , R ⁸ , and R ¹¹ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ -L ⁴ , Y ¹ , Y ² , G ¹ , r, n and m are as defined above.

In certain cases of formula (NB-IID), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IID), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IID), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IID), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IID), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IID), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IID), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIE):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ⁹ , and R ¹⁰ are each independently a chromophore; and L ¹ -L ⁴ , G ¹ , r, n and m are as defined above.

In certain cases of formula (NB-IIE), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIE), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIE), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIE), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIE), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIE), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIE), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIF):

wherein:

R ⁷ , and R ⁸ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ , L ² , L ⁴ , G ¹ , r, n and m are as defined above.

In certain cases of formula (NB-IIF), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIF), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other cases of formula (NB-IIF), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIF), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIF), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIF), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIF), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIG):

wherein:

R ⁷ is a water solubilizing group;

R ¹² is a chromophore; and L ¹ -L ² , G ¹ , r, and n are as defined above.

In certain cases of formula (NB-IIG), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In other cases of formula (NB-IIG), r is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIG), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIG), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIG), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIH):

wherein:

R ⁷ , R ⁸ , and R ¹¹ are each independently a water solubilizing group;

R ¹⁰ is a chromophore; and L ¹ -L ⁴ , G\ r, n and m are as defined above.

In certain cases of formula (NB-IIH), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIH), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIH), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIH), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIH), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIH), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIH), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIIA):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ⁵ , and L ⁶ are each independently a bond or a linker;

Z ¹ , and Z ² are each independently selected from a chromophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n and m are each independently an integer from 1 to 1000;

r is an integer from 0 to 10; and

S ¹ -S ² are each independently an integer from 1 to 50.

In certain cases of formula (NB-IIIA), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIIA), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIIA), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIIA), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIIA), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIIA), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIIA), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIIB):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ⁶ is a bond or a linker;

Z ¹ is selected from a chromophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n and m are each independently an integer from 1 to 1000;

r is an integer from 0 to 10; and

S ¹ -S ² are each independently an integer from 1 to 50.

In certain cases of formula (NB-IIIB), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIIB), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIIB), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIIB), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIIB), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIIB), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-IIIB), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In certain embodiments, the subject multichromophore polymers includes a segment having a formula (NB-IIIC):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ⁷ is a bond or a linker;

Z ³ is selected from a chromophore;

Y ¹ is selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

r is an integer from 0 to 10; and

S ¹ is an integer from 1 to 50.

In certain cases of formula (NB-IIIC), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In other cases of formula (NB-IIIC), r is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIIC), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIIC), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIIC), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain embodiments, the subject multichromophore polymers includes a segment having a formula

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ⁶ is a bond or a linker;

Z ¹ , is selected from a chromophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n and m are each independently an integer from 1 to 1000;

r is an integer from 0 to 10; and

S ¹ -S ³ are each independently an integer from 1 to 50.

In certain cases of formula (NB-IIID), m is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIID), n+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less,

5 mol% or less, or even less. In other cases of formula (NB-IIID), n is 60 mol% or more, such as 65 mol% or more, 70 mol% or more, 75 mol% or more, 80 mol% or more, 85 mol% or more, 90 mol% or more, 95 mol% or more, or even more. In certain instances of formula (NB-IIID), m+r is 40 mol% or less, such as 30 mol% or less, 25 mol% or less, 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In certain instances of formula (NB-IIID), m is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In other instances of formula (NB-IIID), n is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less. In still other instances of formula (NB-II ID), r is 25 mol% or less, such as 20 mol% or less, 15 mol% or less, 10 mol% or less, 5 mol% or less, or even less.

In some embodiments, the subject multichromophore polymers includes a segment having a formula of any one of formulae (NB-II IA) - (NB-IIID) such that any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formulae -C(O)OZ ⁴ or -(CR ³¹ ) _t OC(O)Z ⁴ , wherein: Z ⁴ is a

chromophore; R ³¹ is selected from H, alkyl, substituted alkyl; and t is an integer from 1 to 10. In certain embodiments, any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formula -C(O)OZ ⁴ . In other embodiments, any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formula -CH ₂ OC(O)Z ⁴ . In some instances, G ¹ is a terminal group comprising a functional group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne.

Pendant Chromophore Groups

Any convenient light absorbing chromophore groups can be adapted for use in the subject multichromophores. The terms "light absorbing chromophore group" and "donor chromophore group" are used interchangeably and refer to a pendant group of the

multichromophore capable of absorbing light at a particular absorption maximum wavelength and transferring energy to a proximate chromophore or acceptor fluorophore or converting it to emitted light at a longer emission maximum wavelength.

BODIPY Chromophore Groups

A pendant chromophore group can be a BODIPY group. In some cases, the BODIPY group is a pendant donor chromophore group. The term "BODIPY group" refers to a pendant group of the multichromophore which includes a chromophore having the following boron- dipyrromethene (BODIPY) core structure:

where Q is C or N and each R is any convenient boron substituent. In some cases, Q is C. In some instances, each R is independently selected from F, OH, H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkynyl and substituted alkynyl. The BODIPY core structure may be linked to a repeat unit of the multichromophore via any convenient positions of the core structure, via an optional sidechain linker. The BODIPY core structure may be further optionally substituted. In certain embodiments, the BODIPY group defines a sidechain group of a norbornene repeating unit. Any convenient BODIPY-containing structures may be adapted for use in the subject multichromophores as a BODIPY group.

BODIPY-containing structures of interest include, but are not limited to, those BODIPY dyes and derivatives described by Loudet and Burgess in "BODIPY Dyes and Their Derivatives:

Syntheses and Spectroscopic Properties", Chem. Rev. 2007, 107 (1 1 ): 4891 -4932, Suzuki et at. in U.S. Patent No. 8,193,350, Ulrich et al. in U.S. Patent No. 8,476,461 and Ulrich et ai in U.S. Patent No. 7,897,786, the disclosures of which are herein incorporated by reference in their entirety.

A BODIPY pendant chromophore group can be described by formula (XI):

wherein:

Q is C or N;

R ¹⁴ -R ²⁰ are each independently selected from H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, water solubilizing group (WSG) and -L ⁸ -Z ⁵ , or

optionally any one or more pairs of substituents selected from R ¹⁹ and R ²⁰ , R ¹⁴ and R ¹⁵ , R ¹⁸ and R ¹⁹ , R ¹⁵ and R ¹⁶ , R ¹⁶ and R ¹⁷ and R ¹⁸ and R ¹⁷ , together form a divalent radical and are cyclically linked and together with the carbon atoms to which they are bound provide a 5- or 6- membered fused heterocycle, carbocycle, aryl or heteroaryl ring (e.g., a 5- or 6-membered ring comprising carbon atoms and 0-3 heteroatoms selected from O, S and N), which ring may be unsubstituted or further substituted with a substituent independently selected from alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, water solubilizing group (WSG) and -L ¹ -Z ⁵ ;

L ⁸ is a linker;

Z ⁵ is a norbornene repeat unit of the polymeric backbone; and each R ¹³ are independently selected from F, OH, H, cyano, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and WSG;

wherein one of R ¹³ and R ¹⁴ -R ²⁰ is linked to a norbornene repeat unit of the polymeric backbone. In formula (XI), it is understood that the substituents R ¹³ and R ¹⁴ -R ²⁰ can be selected from groups that do not inhibit the fluorescence of the BODIPY group. In certain instances of formula (XI), Q is C. It is understood that for any of the BODPIY group formulae described herein, a corresponding formula may be included where the atom represented by Q in formula (XI) is a nitrogen atom. In certain embodiments of formula (XI), one or more of R ¹³ and R ¹⁴ -R ²⁰ includes a WSG. In certain instances of formula (XI), each R ¹³ group include a WSG. In certain instances of formula (XI), the linker of -L ⁸ -Z ⁵ includes a WSG.

In formula (XI), the substituent pairs R ¹⁴ and R ¹⁵ and/or R ¹⁹ and R ²⁰ can be cyclically linked to provide a 5- or 6-membered fused ring, which ring is unsubstituted or substituted. In some cases of formula (XI), the 5- or 6-membered fused ring is an aryl or heteroaryl ring selected from furan, thiophene, pyrrole, oxazole, isooxazole, thiazole, isothiazole, imidazole and pyrazole. In some instances of formula (XI), the BODIPY group is of formula (Xlla) or (XIlb):

wherein:

Z ¹¹ and Z ¹² are independently the fused 5- or 6-membered fused heterocycle, carbocycle, aryl or heteroaryl ring;

each "i" is independently 0-3; and

each R ^20a is independently a substituent group as defined for R ¹⁴ -R ²⁰ in formula (X).

In certain embodiments of formulae (Xlla)-(Xllb), Z ¹¹ and Z ¹² are independently a 5- or 6- membered fused aryl or heteroaryl ring. In some cases, Z ¹¹ and Z ¹² are independently selected from furan, thiophene, pyrrole, oxazole, isooxazole, thiazole, isothiazole, imidazole and pyrazole. In some cases of formulae (Xlla)-(Xllb), Z ¹¹ and/or Z ¹² are furan or thiophene. In certain cases of formula (XIla), none of R ¹ -R ⁵ are cyclically linked. In some instances of formulae (Xla)-(XI lb), Z ¹¹ and Z ¹² are independently selected from the following rings:

wherein:

X is O or S;

Y is O, S or NR, wherein R is H, alkyl, substituted alkyl or a substituent as defined for R ²⁰ in formulae (Xlla)-(Xllb); and

R ^21a -R ^23a are independently selected from H and a substituent as defined for R ^20a in formulae (Xlla)-(Xllb). In certain instances of formulae (Xlla)-(Xllb), Z ¹¹ and Z ¹² are

independently selected from the following rings:

as defined above.

In certain cases of formulae (Xllb), Z ¹¹ and Z ¹² are the same rings. In certain instances, Z ¹¹ and Z ¹² include different rings.

In certain embodiments of formula (Xlla), the BODIPY group is of formula (XI I la) or

(XIIIb):

wherein:

X is O or S: and

R ^16a and R ^17a are substituents as defined for R ¹⁹ and R ²⁰ in formula (I).

In certain cases of formula (XIIla) or (XIIIb), none of R ¹⁴ -R ²⁸ are cyclically linked.

In certain embodiments of formula (XI) and (Xllb), the BODIPY group is of formula

(XlVa) or (XlVb):

wherein:

each X is O or S; and

R ^12a , R ^13a , R ^16a and R ^17a and are substituents as defined for R ¹⁴ , R ¹⁵ , R ¹⁹ and R ²⁰ in formula (I).

In certain embodiments of formulae (Xl)-(XIVb), the BOBIPY group includes a linked chemoselective functional group or molecule of interest, such as a light harvesting

multichromophore (e.g., -L ⁸ -Z ⁵ ). In some instances, one of R ^{1 4} -R ²⁰ includes -L ⁸ -Z ⁵ . In certain instances, at least one R ^{1 3} group includes -L ⁸ -Z ⁵ . In certain embodiments of formulae (XI)- (XIVb), R ^{1 7} is -L ⁸ -Z ⁵ where L ⁸ is a linker and Z ⁵ is a chemoselective functional group or a light harvesting multichromophore. In certain embodiments of formulae (Xl)-(XIVb), R ^{1 6} or R ¹⁸ is -L ⁸ - Z ⁵ . In certain embodiments of formulae (XI), (XIla) and (Xllla)-(Xlllb), R ¹⁴ or R ²⁰ is -L ⁸ -Z ⁵ . In certain embodiments of formulae (XI), (XIla) and (Xllla)-(Xlllb), R ¹⁵ or R ¹⁹ is -L ⁸ -Z ⁵ . In certain embodiments of formulae (Xlla)-(Xllb), a R ^20a substituent is -L ⁸ -Z ⁵ . In certain embodiments of formulae (Xllla)-(Xlllb), R ^16a or R ^17a is -L ⁸ -Z ⁵ . In certain embodiments of formulae (XlVa)-(XIVb), R ^12a , R ^13a , R ^16a or R ^17a is -L ⁸ -Z ⁵ .

In some cases, R ¹⁷ is -L ⁸ -Z ⁵ where L ⁸ is a linker (e.g., as described herein) having a backbone of 20 atoms or less in length. In some instances of R ¹⁷ , L ⁸ is selected from an alkyl or substituted alkyl linker, an alkoxy or substituted alkoxy linker, a PEG linker, a sulfonamido-alkyl or substituted sulfonamido-alkyl linker, an amido-alkyl or substituted amido-alkyl linker and an alkyl-amido-alkyl or substituted alkyl-amido-alkyl linker. The linker may be substituted with a WSG, such as a PEG group. In certain instances of R ¹⁷ , L ⁸ is selected from a C ₁ -C ₁₂ alkyl or substituted alkyl linker, a C ₁ -C ₁₂ alkoxy or substituted alkoxy linker, a C ₁ -C ₁₂ amido-alkyl or substituted amido-alkyl linker and a C ₁ -C ₁₂ alkyl-amido-alkyl or substituted alkyl-amido-alkyl linker. In certain instances of R ¹⁷ , Z ⁵ is conjugated to the BODIPY group via a carboxylic acid, an active ester or an alcohol group thereof.

In certain instances of formulae (Xl)-(XIVb), R ^{1 7} includes an optionally substituted carbocyclic or heterocyclic group linked to a repeat unit of the light harvesting multichromophore or to a chemoselective functional group. In certain instances, R ¹⁷ is an optionally substituted aryl or heteroaryl linked to a repeat unit of the light harvesting multichromophore, e.g., via a chemoselective functional group. Bivalent carbocyclic or heterocycle groups of interest include, but are not limited to, 1 ,4-cyclohexyl, 1 ,3-cyclohexyl, piperidinyl (e.g., 1 ,4-piperidinyl), piperazinyl (e.g., 1 ,4-piperazinyl), and the like. Bivalent aryl or heteroaryl groups of interest include, but are not limited to, 1 ,4-phenyl, 1 ,3-phenyl, 2,5-pyridyl, 2,6-pyridyl, 3,5-pyridyl, and the like. The bivalent carbocyclic or heterocycle group or the bivalent aryl or heteroaryl group of R ¹⁷ may be linked to -L ⁹ -Z ⁵ , where L ⁹ is a linking group, e.g., as described in any one of the embodiments herein.

In some embodiments of formula (XI), Q is C and R ¹⁷ -R ²⁰ are each independently selected from H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl and substituted alkynyl. In certain instances of formula (XI), each R ¹³ includes one or more water solubilizing groups (WSGs). In some cases, each R ¹³ are an alkynyl substituted with a WSG. In some cases, each R ¹³ are an alkynyl substituted with a branched WSG. In some instances of formula (XI), each R ¹³ are -CC-CH ₂ )n-O(CH ₂ CH ₂ O)m-R, wherein n is 1 to 6, m is 2 to 50, such as 2 to 30, 2 to 20, 6 to 20, 8 to 20, or 10 to 20, and R is H, alkyl or substituted alkyl (e.g., methyl).

In some embodiments of formula (XI), the BODIPY pendant donor chromophore group can be described by formula (Xla):

wherein:

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ¹³ are alkynyl substituted with one or more WSGs. In some cases, each R ¹³ are alkynyl substituted with a branched WSG. In some instances of formula (Xla), each R ¹³ is -CC- CH ₂ ) _n -O(CH ₂ CH ₂ O)m-R, wherein n is 1 to 6, m is 2 to 50, such as 2 to 30, 2 to 20, 6 to 20, 8 to 20, or 10 to 20, and R is H, alkyl or substituted alkyl (e.g., methyl).

In certain instances of formula (XI), the BODIPY group is of formula (XV):

wherein:

L ⁹ is a covalent bond, oxo (-O-), alkylene (e.g., C ₁ -C ₆ -alkylene), -O-alkylene or a substituted version thereof;

R ^11a is as defined for R ¹⁷ ;

each R ^9a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and t is 0-4. In certain cases of formula (XV), L ³ is a covalent bond. In some cases of formula (XV), L ⁹ is oxo. In certain cases of formula (XV), L ⁹ is a covalent bond.

In certain instances of formula (XV), the BODIPY group is of formula (XVa):

wherein: R ^11a is as defined for R ¹⁷ ; each R ^9a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and t is 0-4. In some cases of formulae (XV)-(XVa), R ^11a is L ⁸ -Z ⁵ (e.g., as described herein). In some cases of formulae (XV)-(XVa), R ^11a includes a sulfonamido-alkyl or substituted sulfonamido-alkyl linker, an amido-alkyl or substituted amido-alkyl linker or an alkyl-amido-alkyl or substituted alkyl-amido-alkyl linker. In certain instances of formulae (XV)-(XVa), R ¹⁴ , R ¹⁶ , R ¹⁸ and R ²⁰ are each independently H, alkyl or substituted alkyl. In certain instances of formulae (XV)-(XVa), R ¹⁵ and R ¹⁹ are each independently H, alkyl or substituted alkyl. In some cases, R ¹⁵ and R ¹⁹ are each H. In certain instances of formulae (XV)-(XVa), R ¹⁴ , R ¹⁶ , R ¹⁸ and R ²⁰ are each independently C ₁ -C ₆ alkyl oerne substituted C ₁ -C ₆ alkyl.

In certain instances of formula (XV), the BODIPY group is of formula (XVIa) or (XVIb):

wherein:

^* is a point of linkage to a repeat unit of the light harvesting multichromophore; and each R ¹³ are independently selected from the group consisting of F, OH, H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkynyl, substituted alkynyl and WSG.

In certain instances of formula (XI), the BODIPY group is of formula (XVIIa) or (XVI lb) :

wherein: n ¹ is 0-12 and Z ⁵ is a chemoselective functional group (e.g., as described herein) or linked molecule of interest. In certain cases of formulae (XVIIa)-(XVIIb), n ¹ is 1 -12 or 1 -6, e.g., 1 , 2, 3, 4, 5 or 6. In certain instances of formulae (XVIIa)-(XVIIb), R ¹⁴ -R ¹⁶ and R ¹⁸ -R ²⁰ are each independently selected from H, C ₁ -C ₆ alkyl and substituted C ₁ -C ₆ alkyl. In certain instances of formulae (XVIIa)-(XVIIb), R ¹⁴ and R ¹⁶ and/or R ¹⁸ and R ²⁰ are each independently selected from C ₁ -C ₆ alkyl and substituted C ₁ -C ₆ alkyl. In certain instances of formulae (XVIIa)- (XVIlb), R ¹⁵ and/or R ¹⁹ are H. In certain instances of formulae (XVIIa)-(XVIIb), R ¹⁵ and/or R ¹⁹ are alkyl or substituted alkyl.

In certain instances of formula (XIIla), the BODIPY group is of formula (XVIII):

wherein: L ⁸ is a linker and Z ⁵ is a linked norbornene repeat unit of the polymeric backbone (e.g., as described herein). In certain instances of formula (XVIII), the BODIPY group is of formula (XVIIIa):

wherein: L ⁹ is a linker (e.g., a linking group component of L ⁸ ); each R ^9a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and t is 0-4.

In certain instances of formula (XlVa), the BODIPY group is of formula (XIX):

wherein: R ^12a and R ^13a are substituents as defined for R ¹⁴ and R ¹⁵ in formula (XI); L ⁸ is a linker and Z ⁵ is a linked norbornene repeat unit of the polymeric backbone (e.g., as described herein). In certain instances of formula (XIX), the BODIPY group is of formula (XlXa):

(XlXa)

wherein: L ⁹ is a linker (e.g., a linking group component of L ⁸ ); each R ^9a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and t is 0-4.

In any of the embodiments of formula (Xl)-(XIXa) described herein, the BODIPY group can include a particular -L ⁸ -Z ⁵ group as described in one of the following embodiments.

L ⁸ can be a linker (e.g., as described herein) having a backbone of 20 atoms or less in length. In some instances, L ⁸ is selected from an alkyl or substituted alkyl linker, an alkoxy or substituted alkoxy linker, a PEG linker, a sulfonamido-alkyl or substituted sulfonamido-alkyl linker, an amido-alkyl or substituted amido-alkyl linker and an alkyl-amido-alkyl or substituted alkyl-amido-alkyl linker. The linker may be substituted with a WSG, such as a PEG group. In certain instances, L ⁸ is selected from a C ₁ -C _{1 2} alkyl or substituted alkyl linker, a C ₁ -C _{1 2} alkoxy or substituted alkoxy linker, a C ₁ -C _{1 2} amido-alkyl or substituted amido-alkyl linker and a C ₁ -C _{1 2} alkyl-amido-alkyl or substituted alkyl-amido-alkyl linker. The linker L can include several linked components, such as one or linking groups independently selected from lower alkylene, substituted lower alkylene, alkenylene (-CH=CH-), substituted alkenylene, alkynylene (-CC-), substituted or unsubstituted amido (e.g., -NRCO- or -CONR-, where R Is H, alkyl or substituted alkyl), substituted or unsubstituted sulfonamido (e.g., -NRSO ₂ - or -SO ₂ NR-, where R Is H, alkyl or substituted alkyl), oxo (-0-), thio (-S-), ethylene glycol (-OCH ₂ CH ₂ O-), polyethylene glycol (e.g., -(CH ₂ CH ₂ O) _n - where n is 2-20, such as 2-10 or 2-6, or 2, 3, 4, 5 or 6), arylene, substituted arylene, heteroarylene, substituted heteroarylene, alpha-amino acid residue, beta-amino acid residue, and the like.

The linked norbornene repeat unit of the polymeric backbone (Z ⁵ ) can be conjugated to the BODIPY group via any convenient chemoselective functional group, e.g., a functional group suitable for conjugation to a molecule of interest having a compatible functional group.

Chemoselective functional groups of interest which find use in linked the subject BODIPY groups to the polymeric backbone include, but are not limited to, amine groups (e.g., -NH ₂ ), carboxylic acid (-CO ₂ H), active ester (e.g., NHS or sulfo-NHS ester), thiol, maleimide, iodoacetamide, hydroxyl, hydrazido, hydrazino, aldehyde, ketone, azido, alkyne, tetrazine, alkene, phosphine and epoxide. It is understood that in some cases, the chemoselective functional group used to link a BODIPY group is a synthetic precursor or protected version of the functional group of interest, which may be converted to a reactive functional group capable of conjugation to the polymeric backbone. For example, a carboxylic acid is a chemoselective functional group which can be coupled with an amine group on a molecule of interest. The carboxylic acid may be converted to an active ester that couples with the amine group, either in situ or prior to coupling.

In some embodiments of formulae (Xl)-(XIXa), L ⁸ includes an optionally substituted carbocyclic or heterocyclic group linked to a norbornene repeat unit of the polymeric backbone. In certain instances, L ⁸ is an optionally substituted aryl or heteroaryl. Bivalent carbocyclic or heterocycle groups of interest include, but are not limited to, 1 ,4-cyclohexyl, 1 ,3-cyclohexyl, piperidinyl (e.g., 1 ,4-piperidinyl), piperazinyl (e.g., 1 ,4-piperazinyl), and the like. Bivalent aryl or heteroaryl groups of interest include, but are not limited to, 1 ,4-phenyl, 1 ,3-phenyl, 2,5-pyridyl, 2,6-pyridyl, 3,5-pyridyl, and the like. The bivalent carbocyclic or heterocycle group or the bivalent aryl or heteroaryl group of L ⁸ may be linked to -L ⁹ -Z ⁵ , where L ⁹ is a linking group, e.g., as described in any one of the embodiments herein. In some embodiments of formulae (l)-(IXa), -L ⁸ -Z ⁵ is described by one of the following structures:

wherein:

R ^11a is L ⁹ -Z ⁵ ;

L ⁹ is a linker and Z ⁵ is a norbornene repeat unit of the polymeric backbone;

t is 0-4; and each R ⁹ is independently selected from alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, halogen, sulfonic acid and water solubilizing group (WSG). In certain embodiments, -L ⁸ -Z ⁵ is described by one of the following structures:

wherein:

L ⁹ is a linker and Z ⁵ is a norbornene repeat unit of the polymeric backbone;

t is 0-4; and each R ^9a is independently selected from alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, halogen, sulfonic acid and water solubilizing group (WSG). In certain embodiments, -L ⁸ -Z ⁵ is described by one of the following structures:

wherein:

L ⁹ is a linker and Z ⁵ is a norbornene repeat unit of the polymeric backbone;

R ^32a is H, alkyl, substituted alkyl, and water solubilizing group (WSG);

L ¹⁰ is a linker selected from alkylene (e.g., C ₁ -C ₆ -alkylene), -O-alkylene and substituted versions thereof;

t is 0-4; and each R ^9a is independently selected from alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxy, aryl, substituted aryl, heteroaryl, substituted heteroaryl, halogen, sulfonic acid and water solubilizing group (WSG).

In any of the embodiments of formulae (Xl)-(XIXa) described herein, the BODIPY group can include particular R ¹³ groups as described in one of the following embodiments. In some cases of formulae (Xl)-(XIXa), one R ¹³ group includes a water solubilizing groups (WSG). In certain cases, R ¹³ is alkynyl substituted with WSG. Each R ¹³ can be the same or different. In some cases of formulae (Xl)-(XIXa), one R ¹³ group is selected from F, OH, H, cyano, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl and substituted alkynyl. In some instances, one R ¹³ group is selected from F, CN, phenyl and substituted phenyl.

In some cases of formulae (Xl)-(XIXa), each R ¹³ group comprises a water solubilizing group (WSG). In certain instances, at least one R ¹³ group is alkynyl substituted with a WSG (e.g., as described herein). In certain instances, at least one R ¹³ group is alkynyl substituted with polyethylene glycol (PEG) or modified (PEG). In certain cases of any of the embodiments of formulae (l)-(IXa), at least one R ¹³ group is of the formula:

wherein: s is 1 to 12;

q ¹ is 0 to 50; and

R ^21a is H, alkyl or substituted alkyl. In certain cases, s is 1 to 6, such as 1 , 2 or 3. In some instances, q is 1 to 50, 1 to 30, 2 to 30, 4 to 30, 6 to 30, 8 to 30, 10 to 30, 10 to 20 or 1 1 to 16. In certain cases, q is 10 to 50, such as 10 to 30, 10 to 20 or 1 1 to 16.

In certain embodiments of formula (XI) and (XVIIa), the BODIPY group has the structure:

where Z ⁵ is a linked norbornene repeat unit of the polymeric backbone; n ¹ is 0-6; each R, R ¹⁴ , R ¹⁶ , R ¹⁸ and R ²⁰ is independently H, C ₁ -C ₆ alkyl or substituted C ₁ -C ₆ alkyl; and each q ¹ is 6-20 (e.g., 10-20). In certain embodiments of formula (XI) and (XVIIa), the BODIPY group has one of the following structures:

In certain embodiments of formulae (XV) and (XVa), the BODIPY dye has the structure:

where Z ⁵ is a linked norbornene repeat unit of the polymeric backbone; n ¹ is 0-6; R, R ¹⁴ , R ¹⁶ , R ¹⁸ and R ²⁰ are each independently H, C ₁ -C ₆ alkyl or substituted C ₁ -C ₆ alkyl; and each q ¹ is 6-20 (e.g., 10-20). In certain embodiments of formulae (XV), (XVa) and (XVIa) or (XVIb), the BODIPY dye has one of the following structures:

In certain embodiments of formulae (XVIII), the BODIPY group has the structure:

where Z ⁵ is a linked norbornene repeat unit of the polymeric backbone; n ¹ is 0-6; each R, R ¹⁴ and R ¹⁶ are independently H, C ₁ -C ₆ alkyl or substituted C ₁ -C ₆ alkyl; and each q is 6-20 (e.g., 10- 20). In certain embodiments of formulae (XVIII), the BODIPY group has one of the following structures:

In certain embodiments of formulae (XlX)-(XIXa), the BODIPY group has the structure:

where Z ⁵ is a linked norbornene repeat unit of the polymeric backbone; n is 0-6; each R and R ^13a are independently H, C ₁ -C ₆ alkyl or substituted C ₁ -C ₆ alkyl; and each q ¹ is 6-20 (e.g., 10-20). In certain embodiments of formulae (XlX)-(XIXa), the BODIPY group has the structure:

In certain embodiments of formulae (Xllla), the BODIPY group has the structure:

In certain embodiments of formulae (XIVa), the BODIPY group has the structure:

In certain embodiments of formulae (XlXa), the BODIPY group has one of the following structures:

In certain embodiments, a BODIPY pendant chromophore group can be described by formula (BD-I):

wherein:

R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group; and

each R ¹³ is selected from the group consisting of F, OH, H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkynyl and substituted alkynyl and a water solubilizing group;

wherein one of R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ is linked to the norbornene repeat unit of the polymeric backbone.

In other embodiments, a BODIPY pendant chromophore group can be described by formula (BD-II):

wherein:

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ^17a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

p is an integer from 0 to 4.

In certain embodiments, the BODIPY pendant chromophore group can be described by the following structure:

Aryl or Heteroaryl Chromophore Groups

The light absorbing chromophore group can be an aryl or heteroaryl chromophore group. Aryl or heteroaryl chromophore groups of interest which find use in the subject

multichromophore norbornene polymers include, but are not limited to, phenyl, biphenyl, benzooxazole, benzothiazole, poly-phenylene, and fused tricyclic groups, such as fluorene, carbazole, silole, biphenyl and bridged biphenyl. The aryl or heteroaryl chromophore groups may be optionally further substituted, e.g., with a water solubilizing group and/or an aryl or heteroaryl substituent that imparts desirable light absorbing properties to the aryl or heteroaryl group.

A fused tricyclic chromophore is a group including a tricyclic aromatic group having three fused rings in a configuration where two aryl or heteroaryl 6-membered rings are fused to a central 5 or 6-membered carbocyclic or heterocyclic ring. In some cases, the fused tricyclic group includes two benzo or pyrido rings fused to a central 5 or 6 membered carbocyclic or heterocyclic ring. The fused tricyclic group can be linked to the sidechain of a co-monomer in the polymeric backbone via any convenient ring atoms of the fused rings. The central 5- or 6- membered ring may be a carbocycle or a heterocycle, aromatic or partially saturated, and may further include a sidechain substituent, e.g., a WSG and/or a linker to a chemoselective tag or the co-monomer sidechain. A bridged biphenyl co-monomer is a fused tricyclic group having a biphenyl group where the two phenyl rings are further linked with each other via a central 6 membered carbocyclic or heterocyclic ring.

In certain instances of the multichromophore norbornene polymer, a pendant donor chromophore group is a fused tricyclic aryl or heteroaryl having one of the following formulae:

wherein:

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

Y is C(R ^13b ) ₂ , -C(R ^13b ) ₂ C(R ^13b ) ₂ -, -C(R ^13b ) ₂ Si(R ^13b ) ₂ -, NR ^13b , Si(R ^13b ) ₂ or Se;

each Z is independently CH, CR or N;

each R ^13b is independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, acyl, substituted acyl, alkoxy, substituted alkoxy, amido, substituted amido, an aralkyl, a substituted aralkyl, a PEG moiety, -L ¹ -Z ⁵ , where L ^{1 1} is a linker and Z ⁵ is a norbornene repeat unit and a WSG, or wherein any two convenient R ^13b groups are optionally cyclically linked; and

each R is independently H or one or more substituents (e.g., WSGs) and wherein any two convenient R groups are optionally cyclically linked. In some cases, one of R and R ^13b is linked to a norbornene repeat unit of the polymeric backbone.

In certain instances, the fused tricyclic group is described by one of the following structures:

wherein Y and each R are as defined above; and the fused tricyclic group can be linked to the norbornene repeat unit of the polymeric backbone via Y or R.

In certain cases, the fused tricyclic group is a fluorene where Y is C(R ^13b ) ₂ . In some cases, the fused tricyclic group is a carbazole where Y is NR ^13b . In some cases, the fused tricyclic group is a silole where Y is Si(R ^13b ) ₂ . In some cases, the fused tricyclic group is a bridged biphenyl where Y is -C(R ^13b ) ₂ C(R ^13b ) ₂ - or is -C(R ^13b ) ₂ Si(R ^13b ) ₂ -. In some cases, the fused tricyclic is a bridged biphenyl where Y is -CHR ^13b CHR ^13b -. In certain instances of any of the fused tricyclic groups described herein, each R is independently selected from H, halogen, alkoxy, substituted alkoxy, alkyl and substituted alkyl. In certain cases, each R is independently selected from H, fluoro, chloro, methoxy, substituted alkoxy, alkyl and substituted alkyl. In certain embodiments of the fused tricyclic group, the group includes two R substituent groups that are cyclically linked to provide a carbocyclic or heterocyclic ring A that is optionally further substituted:

wherein Y is C(R ^13b ) ₂ , -C(R ^13b ) ₂ C(R ^13b ) ₂ -, -C(R ^13b ) ₂ Si(R ^13b ) ₂ -, NR ^13b , Si(R ^13b ) ₂ or Se; and each R ^13b is independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, acyl, substituted acyl, alkoxy, substituted alkoxy, amido, substituted amido, an aralkyl, a substituted aralkyl, a PEG moiety, -L ¹¹ -Z ⁶ , where L ¹¹ is a linker and Z ⁶ is a chemoselective tag (e.g., a tag including a chemoselective functional group) and a WSG; each R is as defined above; and the fused tricyclic group can be linked to the norbornene repeat unit of the polymeric backbone via R ^13b or R. In certain cases, the fused tricyclic group has the structure:

wherein R ^8a -R ^9a are each independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, acyl, substituted acyl, alkoxy, substituted alkoxy, amido, substituted amido, an aralkyl, a substituted aralkyl, a PEG moiety, -L ¹¹ -Z ⁶ , where L ¹¹ is a linker and Z ⁶ is a chemoselective tag (e.g., a tag including a chemoselective functional group) and a WSG; Y and each R is as defined above; and the fused tricyclic group can be linked to the norbornene repeat unit of the polymeric backbone via Y, R ^8a -R ^8a or R. In some cases of the co-monomer, Y is C(R ^13b ) ₂ .

In some embodiments, the chromophore is a Rhodamine or derivative thereof. In certain instances, the Rhodamine is of formula (RH-I):

wherein:

each R ²¹ is selected from hydrogen, alkyl and substituted alkyl;

each R ²² is selected from hydrogen, alkyl and substituted alkyl;

R ²³ , R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ and R ²⁸ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group;

R ²⁹ is selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

q is an integer from 0 to 5;

wherein one of R ²³ R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ , R ²⁸ or R ²⁹ is linked to a norbornene repeat unit of the polymeric backbone.

In certain embodiments, the Rhodamine is of formula (RH-II):

wherein:

* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ^29a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

r is an integer from 0 to 4.

In certain embodiments, the Rhodamine is described by the following structure:

Water Solubilizing Groups

In embodiments, the subject light harvesting multichromophore norbornene polymers are substituted with a plurality of water solubilizing groups (WSG). In some cases, the WSGs are pendant groups connected directly to the norbornene repeat units. In certain cases, the WSGs are substituent groups attached to a pendant donor chromophore or pendant acceptor fluorophore. In some instances, each of the pendant donor chromophore groups are substituted with one or more WSG.

As used herein, the terms "ater solubilizing group" , "water soluble group" and WSG are used interchangeably and refer to a group or substituent that is well solvated in aqueous environments e.g., under physiological conditions, and which imparts improved water solubility upon the molecule to which it is attached. A WSG can increase the solubility of a

multichromophore in a predominantly aqueous solution, as compared to a control

multichromophore which lacks the WSG. The water solubilizing groups may be any convenient hydrophilic group that is well solvated in aqueous environments.

A water soluble multichromophore of the present disclosure has solubility under aqueous conditions that makes it especially suitable for application to a variety of biological assays. The subject water soluble multichromophores and polymeric tandem dyes, and conjugates thereof, can be resistant to undesirable aggregation which provides advantageous fluorescence and spectroscopic properties in various biological assays. Aggregation of dyes is undesirable because it can lead to reduced fluorescent signals, e.g., via aggregation-caused quenching of dye fluorescence. The subject water-soluble multichromophores and polymeric tandem dyes can be used as fluorescent reporters for a variety of biosensors and provide signals of exceptional brightness with a range of options for excitation and emission wavelength for applications such as Flow Cytometry, and imaging.

A variety of water soluble polymer groups can be adapted for use in the WSG of the subject multichromophores. Any convenient water solubilizing groups (WSG’s) may be included in the multichromophores described herein to provide for increased water-solubility. While the increase in solubility may vary, in some instances the increase (as compared to the compound without the WSG(s)) is 2 fold or more, e.g., 5 fold, 10 fold, 25 fold, 50 fold, 100 fold or more. In some cases, the hydrophilic water solubilizing group is charged, e.g., positively or negatively charged. In certain cases, the hydrophilic water solubilizing group is a neutral hydrophilic group. In some embodiments, the WSG is branched (e.g., as described herein). In certain instances, the WSG is linear. In some embodiments, the WSG is a hydrophilic polymer, e.g., a polyethylene glycol, a modified PEG, a peptide sequence, a peptoid, a carbohydrate, an oxazoline, a polyol, a dendron, a dendritic polyglycerol, a cellulose, a chitosan, or a derivative thereof. Water solubilizing groups of interest include, but are not limited to, carboxylate, phosphonate, phosphate, sulfonate, sulfate, sulfinate, sulfonium, ester, polyethylene glycols (PEG) and modified PEGs, hydroxyl, amine, amino acid, ammonium, guanidinium, pyridinium, polyamine and sulfonium, polyalcohols, straight chain or cyclic saccharides, primary, secondary, tertiary, or quaternary amines and polyamines, phosphonate groups, phosphinate groups, ascorbate groups, glycols, including, polyethers, -COOM', -SO ₃ M', -PO ₃ M', -NR ₃ , '

(CH ₂ CH ₂ O) _pp R and mixtures thereof, where Y' can be any halogen, sulfate, sulfonate, or oxygen containing anion, pp can be 1 to 500, each R can be independently H or an alkyl (such as methyl) and M' can be a cationic counterion or hydrogen, --(CH ₂ CH ₂ O) _yy CH ₂ CH ₂ XR ^yy , -- (CH ₂ CH ₂ O) _yy CH ₂ CH ₂ X--, --X(CH ₂ CH ₂ O) _yy CH ₂ CH ₂ -, glycol, and polyethylene glycol, wherein yy is selected from 1 to 1000, X is selected from O, S, and NR ^ZZ , and R ^zz and R ^YY are

independently selected from H and C _1-3 alkyl. In some cases, a WSG is (CH ₂ ) _x (OCH ₂ CH ₂ ) _y OCH ₃ where each x is independently an integer from 0-20, each y is independently an integer from 0 to 50. In some cases, the water solubilizing group includes a non-ionic polymer (e.g., a PEG polymer) substituted at the terminal with an ionic group (e.g., a sulfonate).

In some embodiments of the formulae, the pendant group of interest includes a substituent selected from (CH ₂ ) _x (OCH ₂ CH ₂ ) _y OCH ₃ where each x is independently an integer from 0-20, each y is independently an integer from 0 to 50; and a benzyl optionally substituted with one or more halogen, hydroxyl, C ₁ -C _{1 2} alkoxy, or (OCH ₂ CH ₂ ) _z OCH ₃ where each z is independently an integer from 0 to 50. In some instances, the substituent is

(CH ₂ ) ₃ (OCH ₂ CH ₂ ) ₁₁ OCH ₃ . In some embodiments, one or more of the substituents is a benzyl substituted with at least one WSG groups (e.g., one or two WSG groups) selected from

(CH ₂ ) _x (OCH ₂ CH ₂ ) _y OCH ₃ where each x is independently an integer from 0-20 and each y is independently an integer from 0 to 50.

Multiple WSGs may be included at a single location in the subject multichromophores via a branching linker. In certain embodiments, the branching linker is an aralkyl substituent, further di-substituted with water solubilizing groups. As such, in some cases, the branching linker group is a substituent of the multichromophore that connects the multichromophore to two or more water solubilizing groups. In certain embodiments, the branching linker is an amino acid, e.g., a lysine amino acid that is connected to three groups via the amino and carboxylic acid groups. In some cases, the incorporation of multiple WSGs via branching linkers imparts a desirable solubility on the multichromophore. In some instances, the WSG is a non-ionic sidechain group capable of imparting solubility in water in excess of 50 mg/mL In some instances, the WSG is a non-ionic sidechain group capable of imparting solubility in water in excess of 100 mg/mL. In some embodiments, the multichromophore includes substituent(s) selected from the group consisting of, an alkyl, an aralkyl and a heterocyclic group, each group further substituted with a include water solubilizing groups hydrophilic polymer group, such as a polyethylglycol (PEG) (e.g., a PEG group of 2-20 units).

Water soluble polymers of interest that can be utilized in the WSG include polyethylene glycol (PEG) groups or modified PEG groups. Water-soluble polymers of interest include, but are not limited to, polyalkylene oxide based polymers, such as polyethylene glycol "PEG" (See. e.g., "Poly(thylene glycol) Chemistry: Biotechnical and Biomedical Applications", J. M. Harris, Ed., Plenum Press, New York, N.Y. (1992); and "Poly(ethylene glycol) Chemistry and Biological Applications", J. M. Harris and S. Zalipsky, Eds., ACS (1997); and International Patent

Applications: WO 90/13540, WO 92/00748, WO 92/16555, WO 94/04193, WO 94/14758, WO 94/17039, WO 94/18247, WO 94/28937, WO 95/1 1924, WO 96/00080, WO 96/23794, WO

98/07713, WO 98/41562, WO 98/48837, WO 99/30727, WO 99/32134, WO 99/33483, WO

99/53951 , WO 01/26692, WO 95/13312, WO 96/21469, WO 97/03106, WO 99/45964, and U.S.

Pat. Nos. 4,179,337; 5,075,046; 5,089,261 ; 5,100,992; 5,134,192; 5,166,309; 5,171 ,264;

5,213,891 ; 5,219,564; 5,275,838; 5,281 ,698; 5,298,643; 5,312,808; 5,321 ,095; 5,324,844; 5,349,001 ; 5,352,756; 5,405,877; 5,455,027; 5,446,090; 5,470,829; 5,478,805; 5,567,422; 5,605,976; 5,612,460; 5,614,549; 5,618,528; 5,672,662; 5,637,749; 5,643,575; 5,650,388;

5,681 ,567; 5,686,1 10; 5,730,990; 5,739,208; 5,756,593; 5,808,096; 5,824,778; 5,824,784; 5,840,900; 5,874,500; 5,880,131 ; 5,900,461 ; 5,902,588; 5,919,442; 5,919,455; 5,932,462; 5,965,1 19; 5,965,566; 5,985,263; 5,990,237; 6,01 1 ,042; 6,013,283; 6,077,939; 6,1 13,906; 6,127,355; 6,177,087; 6,180,095; 6,194,580; 6,214,966).

Examples of water soluble polymers of interest include, but are not limited to, those containing a polyalkylene oxide, polyamide alkylene oxide, or derivatives thereof, including polyalkylene oxide and polyamide alkylene oxide comprising an ethylene oxide repeat unit of the formula -(CH ₂ -CH ₂ -O)-. Further examples of polymers of interest include a polyamide having a molecular weight greater than 1 ,000 Daltons of the formula -[C(O)-X-C(O)-NH-Y-NH] _nn - or - [NH-Y-NH-C(O)-X-C(O)] _nn -, where X and Y are divalent radicals that may be the same or different and may be branched or linear, and nn is a discrete integer from 2-100, such as from 2 to 50, and where either or both of X and Y comprises a biocompatible, substantially non- antigenic water-soluble repeat unit that may be linear or branched. Further examples of water- soluble repeat units comprise an ethylene oxide of the formula -(CH ₂ -CH ₂ -O)- or -(O-CH ₂ -CH ₂ )- . The number of such water-soluble repeat units can vary significantly, with the number of such units being from 2 to 500, 2 to 400, 2 to 300, 2 to 200, 2 to 100, 6-100, for example from 2 to 50 or 6 to 50. An example of an embodiment is one in which one or both of X and Y is selected from: -((CH ₂ ) _n2 -(CH ₂ -CH ₂ -O) _n3 -(CH ₂ )- or -((CH ₂ ) _n2 -(O-CH ₂ -CH ₂ ) _n3 -(CH ₂ ) _n-2 -), where n2 is 1 to 6,

1 to 5, 1 to 4, or 1 to 3, and where n3 is 2 to 50, 2 to 25, 2 to 15, 2 to 10, 2 to 8, or 2 to 5. A further example of an embodiment is one in which X is -(CH ₂ -CH ₂ )-, and where Y is -(CH ₂ -(CH ₂ - CH ₂ -O) ₃ -CH ₂ -CH ₂ -CH ₂ )- or -(CH ₂ -CH ₂ -CH ₂ -(O-CH ₂ -CH ₂ ) ₃ -CH ₂ )-.

The term modified polymer, such as a modified PEG, refers to water soluble polymers that have been modified or derivatized at either or both terminals, e.g., to include a terminal substituent (e.g., a terminal alkyl, substituted alkyl, alkoxy or substituted alkoxy, etc.) and/or a terminal linking functional group (e.g., an amino or carboxylic acid group suitable for attachment via amide bond formation) suitable for attached of the polymer to the multichromophore (e.g., via a branching group). The subject water soluble polymers can be adapted to include any convenient linking groups. It is understood that in some cases, the water soluble polymer can include some dispersity with respect to polymer length, depending on the method of preparation and/or purification of the polymeric starting materials. In some instances, the water soluble polymers are monodisperse.

The water soluble polymer can include one or more spacers or linkers. Examples of spacers or linkers include linear or branched moieties comprising one or more repeat units employed in a water-soluble polymer, diamino and or diacid units, natural or unnatural amino acids or derivatives thereof, as well as aliphatic moieties, including alkyl, aryl, heteroalkyl, heteroaryl, alkoxy, and the like, which can contain, for example, up to 18 carbon atoms or even an additional polymer chain.

The water soluble polymer moiety, or one or more of the spacers or linkers of the polymer moiety when present, may include polymer chains or units that are biostable or biodegradable. For example, polymers with repeat linkages have varying degrees of stability under physiological conditions depending on bond lability. Polymers with such bonds can be categorized by their relative rates of hydrolysis under physiological conditions based on known hydrolysis rates of low molecular weight analogs, e.g., from less stable to more stable, e.g., polyurethanes (-NH-C(O)-O-) > polyorthoesters (-0-C((0R)(R’))-O-) > polyamides (-C(O)-NH-). Similarly, the linkage systems attaching a water-soluble polymer to a target molecule may be biostable or biodegradable, e.g., from less stable to more stable: carbonate (-O-C(O)-O-) > ester (-C(O)-O-) > urethane (-NH-C(O)-O-) > orthoester (-O-C((0R)(R’))-O-) > amide (-C(O)- NH-). In general, it may be desirable to avoid use of a sulfated polysaccharide, depending on the lability of the sulfate group. In addition, it may be less desirable to use polycarbonates and polyesters. These bonds are provided by way of example, and are not intended to limit the types of bonds employable in the polymer chains or linkage systems of the water-soluble polymers useful in the WSGs disclosed herein.

In some instances, the WSG is a branched non-ionic water soluble group (WSG) that comprises a branching group linked and provides further linkages to two, three or more non ionic water soluble polymers. In some instances, the branched non-ionic WSG has one of the following formulae:

wherein:

each B ¹ and B ² are independently a branching group;

each W ¹ is independently a non-ionic water soluble polymer, e.g., comprising 6 or more monomeric units;

T ³ is an optional linker to the pendant group or norbornene repeat unit of the

multichromophore polymer; and

each pp and qq are independently 0 or 1 , wherein if present, each T ¹ and each T ² are independently a linker. In certain instances, each W ¹ is independently a PEG or modified PEG polymer. In certain instances, each W ¹ is independently selected from a substituted alkyl, a PEG or modified PEG group and a WSG. In certain instances, each W ¹ is independently a PEG or modified PEG polymer of 6-30 monomeric units, such as 6-24 or 10-30, 10-24 or 10-20, 12-24, 12-20, 12-16 or 16-20 monomeric units.

In some instances, the branched non-ionic WSG has the following formula:

wherein:

each B ¹ is a branching group;

each W ¹ is independently a non-ionic water soluble polymer, e.g., comprising 6 or more monomeric units;

T ³ is an optional linker to the fused 6-5-6 tricyclic co-monomer; and

each pp is independently 0 or 1 , wherein if present, each T ¹ is independently a linker. In certain instances, each W ¹ is independently a PEG or modified PEG polymer. In certain instances, each W ¹ is independently selected from a substituted alkyl, a PEG or modified PEG group and a WSG. In certain instances, each W ¹ is independently a PEG or modified PEG polymer of 6-30 monomeric units, such as 6-24 or 10-30, 10-24 or 10-20, 12-24, 12-20, 12-16 or 16-20 monomeric units. In some embodiments of the branched non-ionic WSG, B ¹ is a tetra- substituted aryl group (e.g., a 1 ,3,4,5-phenyl).

In some embodiments of the branched non-ionic WSG (e.g., as depicted in the formulae above), B ¹ is selected from CH, N, C(=O)N, SO ₂ N, a tri-substituted aryl group (e.g., a 1 ,3,5- phenyl), a tetra-substituted aryl group, and a tri-substituted heteroaryl group. In some embodiments of the branched non-ionic WSG, each pp is 0. In some embodiments of the branched non-ionic WSG, pp is 1 , and each T ¹ is selected from -(CH ₂ ) _n -O-, -O-(CH ₂ ) _n -, - (CH ₂ ) _n — and -O-, wherein n is from 1 to 12, e.g., 1 to 6. In some embodiments of the branched non-ionic WSG, each T ² and/or T ³ is independently a C1 -C12-alkyl linker, e.g., a C1 -C6-alkyl linker, wherein one or more backbone atoms are optionally substituted with a heteroatom.

In some embodiments of the subject multichromophores, the pendant donor

chromophore groups are substituted with one or more water solubilizing groups (WSGs) independently selected from the following formulae:

wherein:

T ⁵ is an optional linker;

each T ⁶ is an linker;

R ³⁰ and R are independently H, alkyl or substituted alkyl; and

each s is an integer from 1 to 100 (e.g., 6 to 100 or 6 to 50).

In certain instances, each s is independently 6 to 30, such as 6 to 24, 6 to 20, 1 1 to 20, 12 to 20, 12 to 18 or 12 to 16. In certain instances, each s is independently 6 to 30, such as 6 to 24, 8 to 24, 10 to 24, 12 to 24, 13 to 24, 14 to 24, 15 to 22 or 16 to 20. In some cases, each s is independently 1 1 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23 or 24. In some embodiments, each s is independently 7 or more, such as 8, 9 or more, 10 or more, 1 1 or more, 12 or more, 13 or more, 14 or more, 15 or more, or even more, and in some cases, have up to 50 monomeric units, such as up to 40, up to 30 or up to 24 monomeric units. In some instances, each s is independently 6-30 monomeric units, such as 6-24 or 10-30, 10-24 or 10-20, 12-24, 12-20, 12- 16 or 16-20 monomeric units. In some cases, each s is the same. In some embodiments of the WSG, T ⁵ and/or T ⁶ is a C1 -C12-alkyl linker, e.g., a C1 -C6-alkyl linker, wherein one or more backbone atoms are optionally substituted with a heteroatom (e.g., an -O-). In some embodiments of the WSG, each R ³⁰ is H. In some embodiments of the WSG, each R ³⁰ is methyl.

It is understood that hydroxy-terminated PEG chains instead of methoxy-terminated PEG chains may be utilized in any of the WSG groups described above. In certain instances of any one of the formulae described herein, one or more of the repeat units or pendant groups is substituted with a WSG that is a dendron selected from one of the following structures:

In certain instances of any one of the formulae described herein, one or more of the repeat units or pendant groups is substituted with a WSG that is a polyol selected from one of the following structures:

In certain instances of any one of the formulae described herein, one or more of the repeat units or pendant groups is substituted with WSG that is an oxazoline of the following

structure:

In certain instances of any one of the formulae described herein, one or more of the repeat units or pendant groups is substituted with a WSG that is a peptoid selected from one of the following structures:

The water soluble group (WSG) can be capable of imparting solubility in water in excess of 10 mg/mL to the multichromophore or polymeric tandem dye, such as in excess of 20 mg/mL, in excess of 30 mg/mL, in excess of 40 mg/mL, in excess of 50 mg/mL, in excess of 60 mg/mL, in excess of 70 mg/mL, in excess of 80 mg/m , in excess of 90 mg/mL or in excess of 100 mg/mL In certain cases, the branched non-ionic water soluble group (WSG) is capable of imparting solubility in water (e.g., an aqueous buffer) of 20 mg/mL or more to the

multichromophore or polymeric tandem dye, such as 30 mg/mL or more, 40 mg/mL or more, 50 mg/mL or more, 60 mg/mL or more, 70 mg/mL or more, 80 mg/mL or more, 90 mg/mL or more, 100 mg/mL or more, or even more. It is understood that water soluble multichromophores may, under certain conditions, form discrete water solvated nanoparticles in aqueous systems. In certain cases, the water solvated nanoparticles are resistant to aggregation and find use in a variety of biological assays.

The norbornene polymeric backbone of the multichromophore may have any convenient length. In some cases, the particular number of norbornene monomeric repeat units of the multichromophore may fall within the range of 2 to 500,000, such as 2 to 100,000, 2 to 30,000, 2 to 10,000, 2 to 3,000 or 2 to 1 ,000 units or segments, or such as 5 to 100,000, 10 to 100,000, 100 to 100,000, 200 to 100,000, or 500 to 50,000 units or segments. In some instances, the particular number of norbornene monomeric repeating units or segments of the polymeric backbone of the multichromophore may fall within the range of 2 to 1 ,000, such as 2 to 500, 2 to 500, 3 to 500, 4 to 500, 5 to 500, 6 to 500, 7 to 500, 8 to 500, 9 to 500, 10 to 500, 10 to 400, 10 to 300, 10 to 200, 10 to 100, or 20 to 100 units or segments.

The multichromophore may be of any convenient molecular weight (MW). In some cases, the MW of the multichromophore may be expressed as an average molecular weight. In some instances, the polymeric dye has an average molecular weight in the range of 500 to 500,000, such as from 1 ,000 to 100,000, from 2,000 to 100,000 (e.g., from 2,000 to 10,000 or from 10,000 to 100,000) or even an average molecular weight in the range of 50,000 to

100,000. In some cases, the norbornene polymeric backbone of the multichromophore is prepared having a particular, discrete sequence of monomers such that the MW of the multichromophore may be expressed as an exact molecular weight. In some instances, the polymeric dye has an exact molecular weight in the range of 500 to 500,000, such as from 1 ,000 to 100,000, from 1 ,000 to 50,000, from 2,000 to 50,000 (e.g., from 2,000 to 10,000 or from 10,000 to 50,000) or even an average molecular weight in the range of 50,000 to 100,000.

POLYMERIC TANDEM DYES

As summarized above, the subject light harvesting multichromophore polymer includes a polymeric backbone of norbornene repeat units that include a plurality of pendant donor chromophore groups, which groups can each be linked to one of the repeat units. As described above, the subject water soluble light harvesting multichromophore polymers are capable of homo-energy transfer between pendant donor chromophores which can lead to continuous reversible energy transfer amongst equal chromophores rather than emission from a single chromophore. As such, although the multichromophore system can itself be fluorescent, via the process of self-quenching, the multichromophore system can have quantum yields that are significantly lower than those observed for a single isolated chromophore.

In some embodiments, the water soluble light harvesting multichromophore is capable of transferring energy to a linked acceptor fluorophore. As such, the subject polymeric tandem dyes further include a covalently linked acceptor signaling fluorophore in energy-receiving proximity to the donor water solvated light harvesting multichromophore system, i.e., in energy- receiving proximity to at least one pendant donor chromophore group. The terms "acceptor fluorophore" and "acceptor chromophore" are used interchangeably herein.

The acceptor signaling fluorophore can be linked to a norbornene repeat unit of the polymeric backbone as a pendant group. Excitation of the multichromophore donor can leads to energy transfer to, and emission from, the covalently attached acceptor signaling fluorophore. The number of repeat units of the donor water solvated light harvesting multichromophore having linked acceptor signaling fluorophore groups may vary, where in some instances the number ranges from 1 mol% to 50 mol% of the repeat units, such as from 1 mol% to 25 mol%, 2 mol% to 25 mol%, 3 mol% to 25 mol%, 4 mol% to 25 mol%, 5 mol% to 25 mol% or from 10 mol% to 25 mol%.

Mechanisms for energy transfer between the light harvesting chromophores of the multichromophore and from these donor chromophores to a linked acceptor signaling fluorophore include, for example, resonant energy transfer (e.g., Forster (or fluorescence) resonance energy transfer, FRET), quantum charge exchange (Dexter energy transfer) and the like. These energy transfer mechanisms can be relatively short range; that is, close proximity of chromophores of the light harvesting multichromophore system to each other and/or to an acceptor fluorophore provides for efficient energy transfer.

Under conditions for efficient energy transfer, amplification of the emission from the acceptor fluorophore can occur where the emission from the luminescent acceptor fluorophore is more intense when the incident light (the "pump light") is at a wavelength which is absorbed by, and transferred from, the chromophores of the light harvesting multichromophore than when the luminescent acceptor fluorophore is directly excited by the pump light.

By "efficient" energy transfer is meant 10% or more, such as 20% or more or 30% or more, 40% or more, 50 % or more, of the energy harvested by the donor chromophores is transferred to the acceptor. By "amplification" is meant that the signal from the acceptor fluorophore is 1.5x or greater when excited by energy transfer from the donor light harvesting multichromophore system as compared to direct excitation of the acceptor fluorophore with incident light of an equivalent intensity. The signal may be measured using any convenient method. In some cases, the 1.5x or greater signal refers to an intensity of emitted light. In certain cases, the 1.5x or greater signal refers to an increased signal to noise ratio. In certain embodiments of the polymeric tandem dye, the acceptor fluorophore emission is 1 .5 fold greater or more when excited by the multichromophore as compared to direct excitation of the acceptor fluorophore with incident light, such as 2-fold or greater, 3-fold or greater, 4-fold or greater, 5- fold or greater, 6-fold or greater, 8-fold or greater, 10-fold or greater, 20-fold or greater, 50-fold or greater, 100-fold or greater, or even greater as compared to direct excitation of the acceptor fluorophore with incident light.

Any convenient fluorescent dyes may be utilized in the polymeric tandem dyes as an acceptor fluorophore. The terms "fluorescent dye" and "fluorophore" are used interchangeably herein. The acceptor fluorophore can be a small molecule fluorophore. The acceptor

fluorophore can be a dye molecule selected from a rhodamine, a coumarin, a xanthene, a cyanine, a polymethine, a pyrene, a thiazine, an acridine, a dipyrromethene borondifluoride, a napthalimide, a phycobiliprotein, a peridinum chlorophyll protein, conjugates thereof, and combinations thereof. In certain embodiments, the acceptor fluorophore is a cyanine dye, a xanthene dye, a coumarin dye, a thiazine dye or an acridine dye. In some instances, the acceptor fluorophore is selected from DY 431 , DY 485XL, DY 500XL, DY 610, DY 640, DY 654, DY 682, DY 700, DY 701 , DY 704, DY 730, DY 731 , DY 732, DY 734, DY 752, DY 778, DY 782, DY 800, DY 831 , Biotium CF 555, Cy 3.5 and diethylamino coumarin. Fluorescent dyes of interest include, but are not limited to, fluorescein, 6-FAM, rhodamine, Texas Red,

tetramethylrhodamine, carboxyrhodamine, carboxyrhodamine 6G, carboxyrhodol,

carboxyrhodamine 1 10, Cascade Blue, Cascade Yellow, coumarin, Cy2, Cy3, Cy3.5, Cy5,

Cy5.5, Cy-Chrome, phycoerythrin, PerCP (peridinin chlorophyll-a Protein), PerCP-Cy5.5, JOE (6-carboxy-4',5'-dichloro-2 ^, ,7 ^, -dimethoxyfluorescein), NED, ROX (5-(and-6)-carboxy-X- rhodamine), HEX, Lucifer Yellow, Marina Blue, Oregon Green 488, Oregon Green 500, Oregon Green 514, Alexa Fluor 350, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 633, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 700, 7-amino-4-methylcoumarin-3-acetic acid, BODIPY FL, BODIPY FL- Br.sub.2, BODIPY 530/550, BODIPY 558/568, BODIPY 564/570, BODIPY 576/589, BODIPY 581/591 , BODIPY 630/650, BODIPY 650/665, BODIPY R6G, BODIPY TMR, BODIPY TR, conjugates thereof, and combinations thereof. Lanthanide chelates of interest include, but are -ot limited to, europium chelates, terbium chelates and samarium chelates. In some embodiments, the polymeric tandem dye includes a multichromophore linked to an acceptor fluorophore selected from Cy3, Cy3.5, Cy5, Cy5.5, Cy7, Alexa488, Alexa 647 and Alexa700. In certain embodiments, the polymeric tandem dye includes a multichromophore linked to an acceptor fluorophore selected from Dyomics dyes (such as DY 431 , DY 485XL, DY 500XL, DY 530, DY 610, DY 633, DY 640, DY 651 , DY 654, DY 682, DY 700, DY 701 , DY 704, DY 730, DY 731 , DY 732, DY 734, DY 752, DY 754, DY 778, DY 782, DY 800 or DY 831 ), Biotium CF 555, Cy 3.5, and diethylamino coumarin.

In some instances, the acceptor fluorophore is a BODIPY group, e.g., a BODIPY group as described above. It is understood that any convenient BODIPY group described herein having a suitable absorption and emission profile can be configured as an acceptor fluorophore in energy receiving proximity to the donor water solvated light harvesting multichromophore system, i.e., in energy-receiving proximity to at least one compatible pendant donor

chromophore group.

In some embodiments, the acceptor fluorophore that is selected has an emission maximum wavelength in the range of 300 to 900 nm, such as 350 to 850 nm, 350 to 600 nm,

360 to 500 nm, 370 to 500 nm, 380 to 500 nm, 390 to 500 nm or 400 to 500 nm, where specific examples of emission maxima of signaling chromophore of interest include, but are not limited to: 395 nm ± 5nm, 420nm ± 5nm, 430nm ± 5nm, 440nm ± 5nm, 450nm ± 5nm, 460nm ± 5nm, 470nm ± 5nm, 480nm ± 5nm, 490nm ± 5nm, 500nm ± 5nm, 510nm ± 5nm, 520nm ± 5nm,

530nm ± 5nm, 540nm ± 5nm, 550nm ± 5nm, 560nm ± 5nm, 570nm ± 5nm, 580nm ± 5nm,

590nm ± 5nm, 605nm ± 5nm, 650nm ± 5nm, 680nm ± 5nm, 700nm ± 5nm, 805nm ± 5nm.

The linked luminescent acceptor fluorophore emission of the polymeric tandem dye can have a quantum yield of 0.03 or more, such as a quantum yield of 0.04 or more, 0.05 or more, 0.06 or more, 0.07 or more, 0.08 or more, 0.09 or more, 0.1 or more, 0.15 or more, 0.2 or more, 0.3 or more or even more. In some instances, the polymeric tandem dye has an extinction coefficient of 5 x 10 ⁵ cm ^-1 M or more, such as 6 x 10 ⁵ cm ^-1 M or more, 7 x 10 ⁵ cm ^-1 M or more, 8 x 10 ⁵ cm ^-1 M or more, 9 x 10 ⁵ cm ^-1 M or more, such as 1 x 10 ⁶ cm ^-1 M or more, 1 .5 x 10 ⁶ cm- ¹ M ^-1 or more, 2 x 10 ⁶ cm ^-1 M or more, 2.5 x 10 ⁶ cm ^-1 M or more, 3 x 10 ⁶ cm ^-1 M or more, 4 x 10 ⁶ cm ^-1 M or more, 5 x 10 ⁶ cm ^-1 M or more, 6 x 10 ⁶ cm ^-1 M or more, 7 x 10 ⁶ cm ^-1 M or more, or 8 x 10 ⁶ cm ^-1 M or more. In some embodiments, the polymeric tandem dye has a molar extinction coefficient of 5 x 10 ⁵ M ^{- 1} cm ^-1 or more. In certain embodiments, the polymeric tandem dye has a molar extinction coefficient of 1 x 10 ⁶ M ^-1 cm ^-1 or more. The subject polymeric tandem dyes can provide for fluorescence emissions from luminescent signaling chromophore dyes that are brighter than the emissions which are possible from such luminescent dyes in isolation. The linked luminescent signaling chromophore emission of the polymeric tandem dye can have a brightness of 50 mM ^-1 cm ^-1 or more, such as 60 mlVMcnT ¹ or more, 70 mlVMcnT ¹ or more, 80 mM ^-1 cm ^-1 or more, 90 mlVMcnT ¹ or more, 100 mM ^-1 cm ^-1 or more, 150 mM ^-1 cm ^-1 or more, 200 mM ^-1 cm ^-1 or more, 250 mM ^-1 cm ^-1 or more, 300 mM ^-1 cm ^-1 or more, or even more. In certain instances, the linked signaling chromophore emission of the polymeric tandem dye has a brightness that is at least 5-fold greater than the brightness of a directly excited luminescent dye, such as at least 10-fold greater, at least 20-fold greater, at least 30-fold greater, at least 50-fold greater, at least 100-fold greater, at least 300- fold greater, or even greater than the brightness of a directly excited luminescent dye.

The subject polymeric tandem dyes can provide for fluorescence emissions having a Stokes shift of 100 nm or more, such as 1 10nm or more, 120nm or more, 130nm or more,

140nm or more, or 150nm or more. In some cases, the Stokes shift is 300nm or less, such as 200nm or less.

In some embodiments, a polymeric tandem dye includes a light harvesting chromophore having a polymeric backbone comprising norbornene repeat units; a pendant water solubilizing (WSG) component; and a pendant donor chromophore component, such that each water solubilizing group and each chromophore group are linked to a norbornene repeat unit of the polymeric backbone; and an energy acceptor comprising a pendant acceptor fluorophore covalently bonded to the polymeric backbone in energy-receiving proximity to the donor chromophore. In certain embodiments, the a polymeric tandem dye includes a water soluble light harvesting multichromophore polymer of any one of formulae (NB-I)-(NB-I II) and an acceptor fluorophore group that is covalently bonded to the norbornene polymeric backbone in energy-receiving proximity to the donor chromophore. It is understood that any of the embodiments of the subject multichromophores of formulae (NB-I)-(NB-III) can also be practiced for a polymeric tandem dye of the present disclosure.

In certain instances, tandem dye polymers include a polymer segment of formula (NB-

VA):

wherein:

S ¹ and S ³ are each independently an integer from 1 to 50. In some instances, Y ¹ and Y ² are each CH ₂ . In some instances, L ¹ , L ² , L ³ and L ⁴ are each independently a linker selected from alkyl, substituted alkyl, acyl, substituted acyl, alkoxy, substituted alkoxy, amino, substituted amino, hydroxyl, an alkyl-amido-alkyl, or a PEG moiety. In some instances, -L ² -R ³³ , and -L ⁴ -R ³⁴ are each independently described by the formulae -C(O)OR ³⁶ and -(CR ³¹ ) _t OC(O)R ³⁶ , wherein: R ³⁶ is selected from a donor chromophore and an acceptor fluorophore; R ³¹ is selected from H, alkyl, substituted alkyl; and t is an integer from 1 to 10. In other instances, each of -L ² -R ³³ , and - L ⁴ -R ³⁴ are described by the formula -C(O)OR ³⁶ . In still other instances, -L ² -R ³³ is of the formula -C(O)OR ³⁶ , wherein R ³⁶ is a donor chromophore; and -L ⁴ -R ³⁴ is of the formula -CH ₂ OC(O)R ³⁶ , wherein R ³⁶ is an acceptor fluorophore. In yet other instances, G ¹ is a terminal group comprising a group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne.

In certain embodiments, the donor chromophore is a BODIPY, as described in detail above. In other embodiments, the donor chromophore is a Rhodamine as described in detail above. In certain instance, each donor chromophore is a linked BODIPY group (e.g., as described herein). In some cases, each acceptor fluorophore is chosen from DY 431 , DY 485XL, DY 500XL, DY 610, DY 640, DY 654, DY 682, DY 700, DY 701 , DY 704, DY 730, DY 731 , DY 732, DY 734, DY 752, DY 778, DY 782, DY 800, DY 831 , iFluor dyes 350, 405, 488, 514, 532, 594, 660, 680, 700, 710, 790, Tide Fluor dyes 5WS, 7WS, 8ws, ICG, BODIPY-based dyes, Biotium CF 555, Cy 3.5 and diethylamino coumarin. In certain instances of formula (Xa), L ⁸ -D ¹ is selected from the following:

where R ⁴⁴ is H, lower alkyl, substituted lower alkyl and WSG.

Alternatively, in some cases pairs of donor and acceptor dyes can be chosen from the following: Dyomics dyes DY 431 , DY 485XL, DY 500XL, DY 610, DY 640, DY 654, DY 682, DY 700, DY 701 , DY 704, DY 730, DY 731 , DY 732, DY 734, DY 752, DY 778, DY 782, DY 800, DY

831 , Fluor dyes 350, 405, 488, 514, 532, 594, 660, 680, 700, 710, 790, Tide Fluor dyes 5WS, 7WS, 8ws, ICG, BODIPY-based dyes, Biotium CF 555, Cy 3.5 and diethylamino coumarin.

In some embodiments, the pendant acceptor fluorophore is selected from the group consisting of a cyanine dye, a rhodamine dye, a perylene diimide dye, a xanthene dye, a coumarin dye, a polymethine, a pyrene, a dipyrromethene borondifluoride, a napthalimide, a thiazine dye and an acridine dye. In certain instances, the pendant acceptor fluorophore is a dye selected from the group consisting of:

wherein:

each X are independently selected from hydrogen, a lone pair of electrons, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, hydroxyl and halogen; and

each Y are independently selected from hydrogen, a lone pair of electrons, alkyl and substituted alkyl (e.g., alkyl chain terminating in a NHS-ester or maleimide moiety).

Labelled Specific Binding Members

Aspects of the present disclosure include labelled specific binding members. A labelled specific binding member is a conjugate of a subject polymeric dye (e.g., as described herein) and a specific binding member. Any of the polymeric dyes or polymeric tandem dyes described herein may be conjugated to a specific binding member. The specific binding member and the polymeric dye can be conjugated (e.g., covalently linked) to each other at any convenient locations of the two molecules, via an optional linker. In some embodiments, the labelled specific binding member is aggregation resistant. As used herein, by "aggregation-resistant" is meant a labelled specific binding member capable of forming a homogenous aqueous composition without aggregated precipitate at a concentration of 1 mg/ml or more in an aqueous buffer of interest, such as 2mg/ml or more, 3mg/ml or more, 4mg/ml or more, 5mg/ml or more, 6mg/ml or more, 7mg/ml or more, 8mg/ml or more, 9mg/ml or more, 10mg/mL or more or even more of the labelled specific binding member.

In certain embodiments, the labelled specific binding member comprises: a water solvated polymeric dye having a deep ultraviolet excitation spectrum and comprising a segment of TT-conjugated co-monomers and a conjugation-modifying repeat unit; and a specific binding member covalently linked to the multichromophore.

As used herein, the term "specific binding member" refers to one member of a pair of molecules which have binding specificity for one another. One member of the pair of molecules may have an area on its surface, or a cavity, which specifically binds to an area on the surface of, or a cavity in, the other member of the pair of molecules. Thus, the members of the pair have the property of binding specifically to each other to produce a binding complex. In some embodiments, the affinity between specific binding members in a binding complex is characterized by a K _d (dissociation constant) of 10 ^-6 M or less, such as 10 ^-7 M or less, including 10 ^-8 M or less, e.g., 10 ^-9 M or less, 10 ^-10 M or less, 10 ^-11 M or less, 10 ^-12 M or less, 10 ^-13 M or less, 10 ^-14 M or less, including 10 ^-15 M or less. In some embodiments, the specific binding members specifically bind with high avidity. By high avidity is meant that the binding member specifically binds with an apparent affinity characterized by an apparent K _d of 10 x 10 ^-9 M or less, such as 1 x 10 ^-9 M or less, 3 x 10 ^-10 M or less, 1 x 10 ^-10 M or less, 3 x 10 ^-11 M or less, 1 x 10 ^-11 M or less, 3 x 10 ^-12 M or less or 1 x 10 ^-12 M or less.

The specific binding member can be proteinaceous. As used herein, the term

"proteinaceous" refers to a moiety that is composed of amino acid residues. A proteinaceous moiety can be a polypeptide. In certain cases, the proteinaceous specific binding member is an antibody. In certain embodiments, the proteinaceous specific binding member is an antibody fragment, e.g., a binding fragment of an antibody that specific binds to a polymeric dye. As used herein, the terms "antibody" and "antibody molecule" are used interchangeably and refer to a protein consisting of one or more polypeptides substantially encoded by all or part of the recognized immunoglobulin genes. The recognized immunoglobulin genes, for example in humans, include the kappa (k), lambda (I), and heavy chain genetic loci, which together comprise the myriad variable region genes, and the constant region genes mu (u), delta (d), gamma (g), sigma (e), and alpha (a) which encode the IgM, IgD, IgG, IgE, and IgA isotypes respectively. An immunoglobulin light or heavy chain variable region consists of a "framework" region (FR) interrupted by three hypervariable regions, also called "complementarity

determining regions" or "CDRs". The extent of the framework region and CDRs have been precisely defined (see, "Sequences of Proteins of Immunological Interest," E. Kabat et al., U.S. Department of Health and Human Services, (1991 )). The numbering of all antibody amino acid sequences discussed herein conforms to the Kabat system. The sequences of the framework regions of different light or heavy chains are relatively conserved within a species. The framework region of an antibody, that is the combined framework regions of the constituent light and heavy chains, serves to position and align the CDRs. The CDRs are primarily responsible for binding to an epitope of an antigen. The term antibody is meant to include full length antibodies and may refer to a natural antibody from any organism, an engineered antibody, or an antibody generated recombinantly for experimental, therapeutic, or other purposes as further defined below.

Antibody fragments of interest include, but are not limited to, Fab, Fab', F(ab')2, Fv, scFv, or other antigen-binding subsequences of antibodies, either produced by the modification of whole antibodies or those synthesized de novo using recombinant DNA technologies. Antibodies may be monoclonal or polyclonal and may have other specific activities on cells (e.g., antagonists, agonists, neutralizing, inhibitory, or stimulatory antibodies). It is understood that the antibodies may have additional conservative amino acid substitutions which have substantially no effect on antigen binding or other antibody functions.

In certain embodiments, the specific binding member is a Fab fragment, a F(ab') fragment, a scFv, a diabody or a triabody. In certain embodiments, the specific binding member is an antibody. In some cases, the specific binding member is a murine antibody or binding fragment thereof. In certain instances, the specific binding member is a recombinant antibody or binding fragment thereof.

In some embodiments, the labelled specific binding member includes: a water solvated light harvesting multichromophore (e.g., as described herein); and a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith (e.g., as described herein); and a specific binding member covalently linked to the multichromophore. In some instances of the labelled specific binding member, the multichromophore of any of the formula described herein, wherein: G ¹ and G ² are each independently selected from the group consisting of a terminal group (e.g., end group), a linker and a linked specific binding member, wherein at least one of G ¹ and G ² is a linked specific binding member.

In certain embodiments of the formulae described herein, G ¹ and/or G ² is a linker, such as a linker including a functional group suitable for conjugation to a specific binding moiety. It is understood that linkers located at the G ¹ and/or G ² positions of the multichromophore may be selected so as to be orthogonal to any other linkers including chemoselective tags (e.g., as described herein) that may be present at a sidechain of the multichromophore (e.g., at Z ² ). In certain embodiments, an amino functional group or derivative thereof is included at G ¹ and/or G ² and a carboxylic acid functional group or derivative thereof is included at Z ² . In certain embodiments, a carboxylic acid functional group or derivative thereof is included at G ¹ and/or G ² and an amino functional group or derivative thereof is included at Z ² .

In some embodiments of the formulae described herein, at least one of G ¹ and G ² is -L ³ - Z ⁴ where L ³ is a linker (e.g., as described herein) and Z ⁴ is a specific binding member (e.g., as described herein). In some embodiments of formulae described herein, at least one of G ¹ and G ² is -L ³ -Z ³ where L ³ is a linker (e.g., as described herein) and Z ³ is a chemoselective tag (e.g., as described herein). Any convenient chemoselective tag and conjugation chemistries can be adapted for use in the subject multichromophores. Chemoselective tags of interest include, but are not limited to, amine, active ester, maleimide, thiol, sulfur(VI) fluoride exchange chemistry (SuFEX), sulfonyl fluoride, Diels Alder cycloaddition click reagents and click chemistry, tetrazine, transcyclooctene, aldehyde, alkoxylamine, alkynes, cyclooctynes, azide, and the like. In some instances, Z ³ is selected from the group consisting of carboxylic acid, active ester (e.g., N-hydroxy succinimidyl ester (NHS) or sulfo-NHS), amino, maleimide, iodoacetyl and thiol.

Biomolecules of interest include, but are not limited to, polypeptides, polynucleotides, carbohydrates, fatty acids, steroids, purines, pyrimidines, derivatives, structural analogs thereof and combinations thereof. In certain instances, Z ⁴ is an antibody. In some instances, Z ⁴ is an antibody fragment or binding derivative thereof. In some cases, the antibody fragment or binding derivative thereof is selected from the group consisting of a Fab fragment, a F(ab') fragment, a scFv, a diabody and a triabody.

METHODS OF USE

As summarized above, aspects of the present disclosure include methods of evaluating a sample for the presence of a target analyte. Aspects of the method include contacting the sample with a norbornene polymeric dye conjugate that specifically binds the target analyte to produce a labelling composition contacted sample. As used herein, the terms "polymeric dye conjugate" and "labelled specific binding member" are used interchangeably. As such, the polymeric dye conjugate can include: (i) a water solvated polynorbornene polymeric dye (e.g., as described herein); and (ii) a specific binding member (e.g., as described herein). In some instances, the polymeric dye conjugate further comprises a signaling chromophore covalently linked to a multichromophore of the polymeric dye in energy-receiving proximity therewith.

Any convenient method may be used to contact the sample with a polymeric dye conjugate that specifically binds to the target analyte to produce the labelling composition contacted sample. In some instances, the sample is contacted with the polymeric dye conjugate under conditions in which the specific binding member specifically binds to the target analyte, if present. For specific binding of the specific binding member of the conjugate with the target analyte, an appropriate solution may be used that maintains the biological activity of the components of the sample and the specific binding member. The solution may be a balanced salt solution, e.g., normal saline, PBS, Flank’s balanced salt solution, etc., conveniently supplemented with fetal calf serum, human platelet lysate or other factors, in conjunction with an acceptable buffer at low concentration, such as from 5-25 mM. Convenient buffers include HEPES, phosphate buffers, lactate buffers, etc. Various media are commercially available and may be used according to the nature of the target analyte, including dMEM, HBSS, dPBS,

RPMI, Iscove’s medium, etc., in some cases supplemented with fetal calf serum or human platelet lysate. The final components of the solution may be selected depending on the components of the sample which are included.

The temperature at which specific binding of the specific binding member of the conjugate to the target analyte takes place may vary, and in some instances may range from 5° C to 50° C, such as from 10° C to 40° C, 15° C to 40° C, 20° C to 40° C, e.g., 20 ° C, 25 ° C, 30 ° C, 35 ° C or 37 ° C (e.g., as described above). In some instances, the temperature at which specific binding takes place is selected to be compatible with the biological activity of the specific binding member and/or the target analyte. In certain instances, the temperature is 25° C, 30° C, 35° C or 37° C. In certain cases, the specific binding member is an antibody or fragment thereof and the temperature at which specific binding takes place is room temperature (e.g., 25° C), 30° C, 35° C or 37° C. Any convenient incubation time for specific binding may be selected to allow for the formation of a desirable amount of binding complex, and in some instances, may be 1 minute (min) or more, such as 2 min or more, 10 min or more, 30 min or more, 1 hour or more, 2 hours or more, or even 6 hours or more.

Any convenient specific binding members may be utilized in the conjugate. Specific binding members of interest include, but are not limited to, those agents that specifically bind cell surface proteins of a variety of cell types, including but not limited to, stem cells, e.g., pluripotent stem cells, hematopoietic stem cells, T cells, T regulator cells, dendritic cells, B Cells, e.g., memory B cells, antigen specific B cells, granulocytes, leukemia cells, lymphoma cells, virus cells (e.g., HIV cells) NK cells, macrophages, monocytes, fibroblasts, epithelial cells, endothelial cells, and erythroid cells. Target cells of interest include cells that have a convenient cell surface marker or antigen that may be captured by a convenient specific binding member conjugate. In some embodiments, the target cell is selected from HIV containing cell, a Treg cell, an antigen-specific T -cell populations, tumor cells or hematopoetic progenitor cells (CD34+) from whole blood, bone marrow or cord blood. Any convenient cell surface proteins or cell markers may be targeted for specific binding to polymeric dye conjugates in the subject methods. In some embodiments, the target cell includes a cell surface marker selected from a cell receptor and a cell surface antigen. In some cases, the target cell may include a cell surface antigen such as CD1 1 b, CD123, CD14, CD15, CD16, CD19, CD193, CD2, CD25, CD27, CD3, CD335, CD36, CD4, CD43, CD45RO, CD56, CD61 , CD7, CD8, CD34, CD1 c, CD23, CD304, CD235a, T cell receptor alpha/beta, T cell receptor gamma/delta, CD253, CD95, CD20, CD105, CD1 17, CD120b, Notch4, Lgr5 (N-Terminal), SSEA-3, TRA-1 -60 Antigen, Disialoganglioside GD2 and CD71 . Any convenient targets may be selected for evaluation utilizing the subject methods. Targets of interest include, but are not limited to, a nucleic acid, such as an RNA, DNA, PNA, CNA, HNA, LNA or ANA molecule, a protein, such as a fusion protein, a modified protein, such as a phosphorylated, glycosylated, ubiquitinated, SUMOylated, or acetylated protein, or an antibody, a peptide, an aggregated biomolecule, a cell, a small molecule, a vitamin and a drug molecule. As used herein, the term "a target protein" refers to all members of the target family, and fragments thereof. The target protein may be any protein of interest, such as a therapeutic or diagnostic target, including but not limited to: hormones, growth factors, receptors, enzymes, cytokines, osteoinductive factors, colony stimulating factors and immunoglobulins. The term "target protein" is intended to include recombinant and synthetic molecules, which can be prepared using any convenient recombinant expression methods or using any convenient synthetic methods, or purchased commercially. In some embodiments, the polymeric dye conjugates include an antibody or antibody fragment. Any convenient target analyte that specifically binds an antibody or antibody fragment of interest may be targeted in the subject methods.

In some embodiments, the target analyte is associated with a cell. In certain instances, the target analyte is a cell surface marker of the cell. In certain cases, the cell surface marker is selected from the group consisting of a cell receptor and a cell surface antigen. In some instances, the target analyte is an intracellular target, and the method further includes lysing the cell.

In some embodiments, the sample may include a heterogeneous cell population from which target cells are isolated. In some instances, the sample includes peripheral whole blood, peripheral whole blood in which erythrocytes have been lysed prior to cell isolation, cord blood, bone marrow, density gradient-purified peripheral blood mononuclear cells or homogenized tissue. In some cases, the sample includes hematopoetic progenitor cells (e.g., CD34+ cells) in whole blood, bone marrow or cord blood. In certain embodiments, the sample includes tumor cells in peripheral blood. In certain instances, the sample is a sample including (or suspected of including) viral cells (e.g., HIV).

The labelled specific binding members find use in the subject methods, e.g., for labeling a target cell, particle, target or analyte with a polymeric dye or polymeric tandem dye. For example, labelled specific binding members find use in labeling cells to be processed (e.g., detected, analyzed, and/or sorted) in a flow cytometer. The labelled specific binding members may include antibodies that specifically bind to, e.g., cell surface proteins of a variety of cell types (e.g., as described herein). The labelled specific binding members may be used to investigate a variety of biological (e.g., cellular) properties or processes such as cell cycle, cell proliferation, cell differentiation, DNA repair, T cell signaling, apoptosis, cell surface protein expression and/or presentation, and so forth. Labelled specific binding members may be used in any application that includes (or may include) antibody-mediated labeling of a cell, particle or analyte.

In some instances of the method, the labelled specific binding member includes a multichromophore polynorbornene polymer as described herein. In certain cases, G ¹ and G ² are each independently selected from the group consisting of a terminal group, a polymeric segment, a linker, a chemoselective tag and a linked specific binding member, wherein at least one of G ¹ and G ² is a linked specific binding member.

Aspects of the method include assaying the labelling composition contacted sample for the presence of a polymeric dye conjugate-target analyte binding complex to evaluate whether the target analyte is present in the sample. Once the sample has been contacted with the polymeric dye conjugate, any convenient methods may be utilized in assaying the labelling composition contacted sample that is produced for the presence of a polymeric dye conjugate- target analyte binding complex. The polymeric dye conjugate-target analyte binding complex is the binding complex that is produced upon specific binding of the specific binding member of the conjugate to the target analyte, if present. Assaying the labelling composition contacted sample can include detecting a fluorescent signal from the binding complex, if present. In some cases, the assaying includes a separating step where the target analyte, if present, is separated from the sample. A variety of methods can be utilized to separate a target analyte from a sample, e.g., via immobilization on a support. Assay methods of interest include, but are not limited to, any convenient methods and assay formats where pairs of specific binding members such as avidin- biotin or hapten-anti-hapten antibodies find use, are of interest. Methods and assay formats of interest that may be adapted for use with the subject compositions include, but are not limited to, flow cytometry methods, in-situ hybridization methods, enzyme-linked

immunosorbent assays (ELISAs), western blot analysis, magnetic cell separation assays and fluorochrome purification chromatography.

In certain embodiments, the method further includes contacting the sample with a second specific binding member that specifically binds the target analyte. In certain instances, the second specific binding member is support bound. Any convenient supports may be utilized to immobilize a component of the subject methods (e.g., a second specific binding member). In certain instances, the support is a particle, such as a magnetic particle. In some instances, the second specific binding member and the polymeric dye conjugate produce a sandwich complex that may be isolated and detected, if present, using any convenient methods. In some embodiments, the method further includes flow cytometrically analyzing the polymeric dye conjugate-target analyte binding complex, i.e., a fluorescently labelled target analyte. Assaying for the presence of a polymeric dye conjugate-target analyte binding complex may provide assay results (e.g., qualitative or quantitative assay data) which can be used to evaluate whether the target analyte is present in the sample.

Any convenient supports may be utilized in the subject methods to immobilize any convenient component of the methods, e.g., labelled specific binding member, target, secondary specific binding member, etc. Supports of interest include, but are not limited to: solid substrates, where the substrate can have a variety of configurations, e.g., a sheet, bead, or other structure, such as a plate with wells; beads, polymers, particle, a fibrous mesh, hydrogels, porous matrix, a pin, a microarray surface, a chromatography support, and the like. In some instances, the support is selected from the group consisting of a particle, a planar solid substrate, a fibrous mesh, a hydrogel, a porous matrix, a pin, a microarray surface and a chromatography support. The support may be incorporated into a system that it provides for cell isolation assisted by any convenient methods, such as a manually-operated syringe, a centrifuge or an automated liquid handling system. In some cases, the support finds use in an automated liquid handling system for the high throughput isolation of cells, such as a flow cytometer.

In some embodiments of the method, the separating step includes applying an external magnetic field to immobilize a magnetic particle. Any convenient magnet may be used as a source of the external magnetic field (e.g., magnetic field gradient). In some cases, the external magnetic field is generated by a magnetic source, e.g. by a permanent magnet or

electromagnet. In some cases, immobilizing the magnetic particles means the magnetic particles accumulate near the surface closest to the magnetic field gradient source, i.e. the magnet.

The separating may further include one or more optional washing steps to remove unbound material of the sample from the support. Any convenient washing methods may be used, e.g., washing the immobilized support with a biocompatible buffer which preserves the specific binding interaction of the polymeric dye and the specific binding member. Separation and optional washing of unbound material of the sample from the support provides for an enriched population of target cells where undesired cells and material may be removed.

In certain embodiments, the method further includes detecting the labelled target.

Detecting the labelled target may include exciting the multichromophore with one or more lasers and subsequently detecting fluorescence emission from the polymeric tandem dye using one or more optical detectors. Detection of the labelled target can be performed using any convenient instruments and methods, including but not limited to, flow cytometry, FACS systems, fluorescence microscopy; fluorescence, luminescence, ultraviolet, and/or visible light detection using a plate reader; high performance liquid chromatography (HPLC); and mass spectrometry. When using fluorescently labeled components in the methods and compositions of the present disclosure, it is recognized that different types of fluorescence detection systems can be used to practice the subject methods. In some cases, high throughput screening can be performed, e.g., systems that use 96 well or greater microtiter plates. A variety of methods of performing assays on fluorescent materials can be utilized, such as those methods described in, e.g., Lakowicz, J. R., Principles of Fluorescence Spectroscopy, New York: Plenum Press (1983); Herman, B., Resonance energy transfer microscopy, in: Fluorescence Microscopy of Living Cells in Culture, Part B, Methods in Cell Biology, vol. 30, ed. Taylor, D. L. & Wang, Y.-L., San Diego: Academic Press (1989), pp. 219-243; Turro, N.J., Modern Molecular Photochemistry, Menlo Park:

Benjamin/Cummings Publishing Col, Inc. (1978), pp. 296-361.

Fluorescence in a sample can be measured using a fluorimeter. In some cases, excitation radiation, from an excitation source having a first wavelength, passes through excitation optics. The excitation optics cause the excitation radiation to excite the sample. In response, fluorescently labelled targets in the sample emit radiation which has a wavelength that is different from the excitation wavelength. Collection optics then collect the emission from the sample. The device can include a temperature controller to maintain the sample at a specific temperature while it is being scanned. In certain instances, a multi-axis translation stage moves a microtiter plate holding a plurality of samples in order to position different wells to be exposed. The multi-axis translation stage, temperature controller, auto-focusing feature, and electronics associated with imaging and data collection can be managed by an appropriately programmed digital computer. The computer also can transform the data collected during the assay into another format for presentation.

In some embodiments, the method of evaluating a sample for the presence of a target analyte further includes detecting fluorescence in a flow cytometer. In some embodiments, the method of evaluating a sample for the presence of a target analyte further includes imaging the labelling composition contacted sample using fluorescence microscopy. Fluorescence microscopy imaging can be used to identify a polymeric dye conjugate-target analyte binding complex in the contacted sample to evaluate whether the target analyte is present. Microscopy methods of interest that find use in the subject methods include laser scanning confocal microscopy.

Also provided are methods of labelling a target molecule. The subject polymeric dyes, find use in a variety of methods of labelling, separation, detection and/or analysis. In some embodiments, the method includes: contacting the target molecule with a polymeric dye (e.g., as described herein) to produce a labelled target molecule, wherein the polymeric dye includes a conjugation tag that covalently links to the target molecule. In some cases, the polymeric dye further includes a signaling chromophore covalently linked to the multichromophore of the polymeric dye in energy-receiving proximity therewith. In some instances of the method, the polymeric dye member includes a multichromophore polynorbornene polymer as described herein, where one of G ¹ and G ² is a terminal group and the other of G ¹ and G ² is the conjugation tag.

As used herein the term "conjugation tag" refers to a group that includes a

chemoselective functional group (e.g., as described herein) that can covalently link with a compatible functional group of a target molecule, after optional activation and/or deprotection. Any convenient conjugation tags may be utilized in the subject polymeric dyes in order to conjugate the dye to a target molecule of interest. In some embodiments, the conjugation tag includes a terminal functional group selected from an amino, a carboxylic acid or a derivative thereof, a thiol, a hydroxyl, a hydrazine, a hydrazide, a azide, an alkyne and a protein reactive group (e.g. ami no- reactive, thiol-reactive, hydroxyl-reactive, imidazolyl-reactive or guanidinyl- reactive).

Any convenient methods and reagent may be adapted for use in the subject labelling methods in order to covalently link the conjugation tag to the target molecule. Methods of interest for labelling a target, include but are not limited to, those methods and reagents described by Hermanson, Bioconjugate Techniques, Third edition, Academic Press, 2013. The contacting step may be performed in an aqueous solution. In some instances, the conjugation tag includes an amino functional group and the target molecule includes an activated ester functional group, such as a NHS ester or sulfo-NHS ester, or vice versa. In certain instances, the conjugation tag includes a maleimide functional group and the target molecule includes a thiol functional group, or vice versa. In certain instances, the conjugation tag includes an alkyne (e.g., a cyclooctyne group) functional group and the target molecule includes an azide functional group, or vice versa, which can be conjugated via Click chemistry.

Any convenient target molecules may be selected for labelling utilizing the subject methods. Target molecules of interest include, but are not limited to, a nucleic acid, such as an RNA, DNA, PNA, CNA, HNA, LNA or ANA molecule, a protein, such as a fusion protein, a modified protein, such as a phosphorylated, glycosylated, ubiquitinated, SUMOylated, or acetylated protein, or an antibody, a peptide, an aggregated biomolecule, a cell, a small molecule, a vitamin and a drug molecule. As used herein, the term "a target protein" refers to all members of the target family, and fragments thereof. The target protein may be any protein of interest, such as a therapeutic or diagnostic target, including but not limited to: hormones, growth factors, receptors, enzymes, cytokines, osteoinductive factors, colony stimulating factors and immunoglobulins. The term "target protein" is intended to include recombinant and synthetic molecules, which can be prepared using any convenient recombinant expression methods or using any convenient synthetic methods, or purchased commercially. In some embodiments, the target molecule is a specific binding member (e.g., as described herein). In certain instances, the specific binding member is an antibody. In some instances, the specific binding member is an antibody fragment or binding derivative thereof. In some case, the antibody fragment or binding derivative thereof is selected from the group consisting of a Fab fragment, a F(ab') fragment, a scFv, a diabody and a triabody.

In some cases, the method includes a separating step where the labelled target molecule is separated from the reaction mixture, e.g., excess reagents or unlabeled target. A variety of methods may be utilized to separate a target from a sample, e.g., via immobilization on a support, precipitation, chromatography, and the like.

In some instances, the method further includes detecting and/or analyzing the labelled target molecule. In some instances, the method further includes fluorescently detecting the labelled target molecule. Any convenient methods may be utilized to detect and/or analyze the labelled target molecule in conjunction with the subject methods and compositions. Methods of analyzing a target of interest that find use in the subject methods, include but are not limited to, flow cytometry, fluorescence microscopy, in-situ hybridization, enzyme-linked immunosorbent assays (ELISAs), western blot analysis, magnetic cell separation assays and fluorochrome purification chromatography. Detection methods of interest include but are not limited to fluorescence spectroscopy, fluorescence microscopy, nucleic acid sequencing, fluorescence in- situ hybridization (FISH), protein mass spectroscopy, flow cytometry, and the like.

Detection may be achieved directly via the polymeric dye or polymeric tandem dye, or indirectly by a secondary detection system. The latter may be based on any one or a

combination of several different principles including, but not limited to, antibody labelled anti species antibody and other forms of immunological or non-immunological bridging and signal amplification systems (e.g., biotin-streptavidin technology, protein-A and protein-G mediated technology, or nucleic acid probe/anti-nucleic acid probes, and the like). Suitable reporter molecules may be those known in the field of immunocytochemistry, molecular biology, light, fluorescence, and electron microscopy, cell immunophenotyping, cell sorting, flow cytometry, cell visualization, detection, enumeration, and/or signal output quantification. More than one antibody of specific and/or non-specific nature might be labelled and used simultaneously or sequentially to enhance target detection, identification, and/or analysis.

METHODS OF PREPARATION

Aspects of the present disclosure include methods of preparing the subject

multichromophores and polymeric tandem dyes. Methods according to certain embodiments, include contacting a first norbornene monomer comprising a pendant chromophore component with a second norbornene monomer comprising a pendant water solubilizing group component to generate a polymeric backbone comprising a norbornene repeat unit, a pendant water solubilizing group (WSG) component and a pendant chromophore component.

Any convenient polymer synthesis methods can be utilized in the preparation of the polymeric backbones of the subject multichromophores and polymeric tandem dyes. In some cases, ring-opening metathesis polymerization (ROMP) methods are utilized to prepare polymeric backbones of the subject multichromophores via a defined stepwise synthesis.

Conventional ROMP strategies provide for coupling of first and second norbornene monomer units of interest in a controlled manner, to generate a polymeric backbone comprising a norbornene repeat unit. In some cases, the first norbornene monomer unit comprises a pendant chromophore component. In some cases, the first norbornene monomer unit comprises a pendant chromophore component and a WSG group. In some cases, the second norbornene monomer unit comprises a pendant WSG component. In some cases, the second norbornene monomer unit comprises a pendant WSG component and a pendant chromophore component. In certain instances, one or more of the pendant groups can be installed into the first and second norbornene monomer starting materials. In some cases, the pendant groups are installed into the multichromophore after ROMP of the polymeric backbone has been performed. By use of distinct first and second norbornene units, different groups of interest can be independently installed onto the polypeptide backbone.

In some embodiments, the method of preparing subject multichromophores and polymeric tandem dyes (e.g., as described herein) includes synthesizing a first norbornene monomer unit comprising a pendant chromophore component; and a second norbornene monomer unit comprising a pendant WSG component. In will be understood that any convenient synthesis methods can be utilized in the preparation of the first and second norbornene monomer units. Many general references providing commonly known chemical synthetic schemes and conditions useful for synthesizing exemplary first and second

norbornene monomer units are available (see, e.g., Smith and March, March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, Fifth Edition, Wiley-lnterscience, 2001 ; or Vogel, A Textbook of Practical Organic Chemistry, Including Qualitative Organic Analysis, Fourth Edition, New York: Longman, 1978). Reactions may be monitored by thin layer chromatography (TLC), LC/MS and reaction products characterized by LC/MS and ¹ H NMR. Intermediates and final products may be purified by silica gel chromatography or by HPLC.

A variety of additional pendant groups including acceptor fluorophores can then be installed sequentially via selective conjugation(s) to chemoselective tag(s) attached to particular locations on the first and/or second norbornene monomer units.

As described herein, the preparation of a subject polymeric backbone can provide for a desired configuration of pendant donor chromophores, and in some cases, acceptor

fluorophores along the polypeptide. When installation of the pendant groups is achieved post polymer synthesis, chemoselective functional groups of the norbornene units along the polypeptide backbone are selectively conjugated to pendant groups.

In certain instances, the subject multichromophore or polymeric tandem dye has a norbornene polymeric backbone wherein: each block of first norbornene monomer unit and second norbornene monomer unit comprises at least one pendant chromophore; and at least one pendant WSG. In certain cases, each block of first norbornene monomer unit and second norbornene monomer unit comprises one pendant chromophore; and at least one pendant WSG. In certain cases, each block of first norbornene monomer unit and second norbornene monomer unit further comprises a pendant acceptor fluorophore group.

Accordingly, in some cases, the method further includes coupling reactive acceptor fluorophore moieties to a chemoselective sidechain groups of the second norbornene monomer units to produce norbornene monomer units with pendant acceptor fluorophores.

Any of the subject multichromophore or polymeric tandem dye described herein can be prepared according to the subject methods, e.g., dyes of formulae (NB-I)-(NB-IH), (NB-II)-(NB- I IH), (NB-III)-(NBIIID), (NB-IV), (NB-V)-(NB-VA) and synthetic precursors thereof, as described herein. In certain cases, the multichromophore prepared in accordance with the subject methods is described by the formula (NB-II) (e.g., as described herein). In certain cases, the polymeric tandem dye prepared in accordance with the subject methods is described by the formula (NB-IV) (e.g., as described herein). In certain embodiments of the subject methods of preparation, the second norbornene monomer further comprises a pendant chromophore component. In certain cases, the second monomer further comprises an acceptor fluorophore group and the contacting generates a polymeric backbone comprising a pendant water solubilizing group (WSGs) component, a pendant chromophore component and a pendant acceptor fluorophore group.

In certain embodiments of the subject methods of preparation, the first norbornene monomer is contacted with the second norbornene monomer in the presence of a catalyst and an alkene.

In certain embodiments of the subject methods of preparation, the catalyst is a ruthenium complex. In certain cases, the ruthenium complex is a Grubbs catalyst. In certain cases, the Grubbs catalyst is a first generation Grubbs catalyst. In certain cases, the Grubbs catalyst is a second generation Grubbs catalyst. In still other cases, the Grubbs catalyst is a third generation Grubbs catalyst. Suitable catalysts for use in the subject methods of preparation include, but are not limited to those described in Grubbs, R. H., Handbook of Metathesis (1 ^st ed.)., Weinheim: Wiley-VCH (2003); Grubbs, et al,“Ruthenium-Catalyzed Olefin Metathesis”. In Murashashi, S,. Ruthenium in Organic Synthesis. Weinheim: Wiley-VCH. Pp.. 153-177 (2004)·, and US Patent No. 5,728,785; the disclosures of which are incorporated herein by reference.

In certain cases of the subject methods of preparation, the alkene is of the formula

CH ₂ =CH(CH ₂ ) _U G ¹ wherein: G ¹ is selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member; and u is an integer from 0 to 10. In certain cases, G ¹ is a terminal group comprising a group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne. In certain cases, G ¹ is a tert-butyl N-allyl carbamate derivative.

In certain embodiments of the subject methods of preparation, the first monomer and second monomer are contacted at a temperature from -20 °C to 220 °C. In some cases, the temperature is from -20 °C to 200 °C, such as -20 °C to 180 °C, -20 °C to 160 °C, -20 °C to 140 °C, -20 °C to 120 °C, -20 °C to 100 °C, -20 °C to 80 °C, -20 °C to 60 °C, -20 °C to 40 °C, -20 °C to 20 °C, or -20 °C to 0 °C. In some cases, the temperature is from 0 °C to 200 °C, such as 20 °C to 200 °C, 40 °C to 200 °C, 60 °C to 200 °C, 80 °C to 200 °C, 100 °C to 200 °C, 120 °C to 200 °C, 140 °C to 200 °C, 160 °C to 200 °C, or 180 °C to 200 °C. In certain embodiments of the subject methods of preparation, the first monomer and second monomer are contacted at a pressure from 0.01 atm to 10 atm. In some cases the pressure is from, 0.01 atm to 9 atm, such as 0.01 atm to 9 atm, 0.01 atm to 8 atm, 0.01 atm to 7 atm, 0.01 atm to 6 atm, 0.01 atm to 5 atm, 0.01 atm to 4 atm, 0.01 atm to 3 atm, 0.01 atm to 2 atm, or 0.01 atm to 1 atm. In some cases, the pressure is from 0.01 atm to 1 atm, such as 0.01 atm to 0.9 atm, 0.01 atm to 0.8 atm, 0.01 atm to 0.7 atm, 0.01 atm to 0.6 atm, 0.01 atm to 0.5 atm, 0.01 atm to 0.4 atm, 0.01 atm to 0.3 atm, 0.01 atm to 0.2 atm or 0.01 atm to 0.1 atm.

In certain embodiments of the subject methods of preparation, the first monomer and the second monomer are contacted in a solvent. In certain cases, the solvent is a single solvent. In other cases, the solvent is a mixture of solvents. In certain cases, the solvent is selected from dichloromethane, chloroform, dimethylacetamide, toluene, xylene, benzene, dimethyl formamide, acetonitrile and dimethyl sulfoxide, or any combination thereof.

In certain embodiments, the methods of preparing the subject multichromophores is described by Scheme 1 :

Scheme 1: General synthetic method for the preparation of the subject multichromophores.

With reference to scheme 1 , a first norbornene monomer unit of formula (NB-ll-1 ) is contacted with a second norbornene monomer unit of formula (NB-ll-2) in the presence of a solvent, a catalyst and an alkene (e.g., as described herein) at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide a multichromophore of formula (NB-II).

In certain embodiments of the first norbornene monomer unit of formula (NB-ll-1 ), one of R ⁴ or R ³ is a pendant chromophore component. In certain cases, R ⁴ is hydrogen, and R ³ is a pendant chromophore component. In certain cases, R ⁴ is a WSG (e.g., as described herein), and R ³ is a pendant chromophore component. In certain cases, R ³ and R ⁴ are both pendant chromophore components.

In certain embodiments of the second norbornene monomer unit of formula (NB-ll-2), one of R ¹ or R ² is a pendant WSG component. In certain cases, R ¹ is hydrogen, and R ² is a WSG component. In certain cases, R ¹ is a WSG (e.g., as described herein), and R ² is a pendant chromophore component. In certain cases, R ¹ and R ² are both pendant WSG components.

In certain embodiments of Scheme one, the first norbornene monomer unit and the second norbornene unit are the same, such that the method of preparation may be described by Scheme 2:

Scheme 2: Synthetic method for the preparation of the subject multichromophores of formula (NB-IIC)

With reference to scheme 2, norbornene monomer units of formula (NB-ll-3), wherein R ⁷ is a pendant WSG component, and R ¹² is a pendant chromophore, are reacted in the presence of a solvent, a catalyst and an alkene (e.g., as described herein) at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to produce a multichromophore of formula (NB-IIC).

In certain embodiments, the methods of preparing the subject polymeric tandem dyes is described by Scheme 3:

Scheme 3: General synthetic method for the preparation of the subject polymeric tandem dyes.

With reference to scheme 3, a first norbornene monomer unit of formula (NB-V-1 ) is contacted with a second norbornene monomer unit of formula (NB-V-2) in the presence of a solvent, a catalyst and an alkene (e.g., as described herein) at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide a multichromophore of formula (NB-V).

In certain embodiments of the first norbornene monomer unit of formula (NB-V-1 ), R ³² is a WSG component and R ³³ is a donor chromophore component; and in the second norbornene monomer unit of formula (NB-V-2), R ³⁴ is a WSG component and R ³⁵ is an acceptor fluorophore component (e.g., as described herein). In other cases, of the first norbornene monomer unit of formula (NB-V-1 ), R ³² is a WSG component and R ³³ is an acceptor fluorophore; and in the second norbornene monomer unit of formula (NB-V-2), R ³⁴ is a WSG component and R ³⁵ is a donor chromophore component (e.g., as described herein). In certain cases R ³³ or R ³⁵ is a donor chromophore component including, but not limited to, a BODIPY derivative (e.g., as described herein). In certain cases, R ³³ or R ³⁵ is an acceptor fluorophore component including, but not limited to, Rhodamine B, Rhodamine 6G, perylene diimide or cyanine 3.

In the subject methods of preparation, a variety of moieties can be included as G ¹ in the alkene component of the reaction. G ¹ can be any convenient terminal group (e.g., a capping group such as an alkanoyl, e.g., acetyl), a donor chromophore group, a linker having a particular chemoselective tag or a biomolecule of interest.

In some cases, the method further includes installing a second pendant G ¹ group at a convenient position on the polymeric backbone. This can be achieved in a variety of ways: e.g.; post synthesis where a moiety of interest (e.g., a specific binding member) or a particular chemoselective tag directed to same, can be coupled to the compatible group on the polymeric backbone. In some instances, the compatible group is part of a pendant WSG component. In some instances, the compatible group is part of the pendant chromophore component.

SYSTEMS

Aspects of the invention further include systems for use in practicing the subject methods and compositions. A sample analysis system can include sample field of view or a flow channel loaded with a sample and a labelled specific binding member. In some

embodiments, the system is a flow cytometric system including: a flow cytometer including a flow path; a composition in the flow path, wherein the composition includes: a sample; and a labelled specific binding member (e.g., as described herein). In some embodiments, the system for analyzing a sample is a fluorescence microscopy system, including: a fluorescence microscope comprising a sample field of view; and a composition disposed in the sample field of view, wherein the composition comprises a sample; and a labelled specific binding member (e.g., as described herein).

In some instances of the systems, the labelled specific binding member includes: a water solvated light harvesting multichromophore polynorbornene polymer (e.g., as described herein) and a specific binding member that specifically binds a target analyte covalently linked to the multichromophore. In some cases, the labelled specific binding member further comprises a signaling chromophore covalently linked to the multichromophore of the polymeric dye in energy-receiving proximity therewith. In some instances of the subject systems, the labelled specific binding member, the multichromophore is described by any one of formulae (NBI)-(NB-III) (e.g., as described herein), wherein: G ¹ and G ² are each independently selected from the group consisting of a terminal group, a polymeric conjugated segment, a linker and a linked specific binding member, wherein at least one of G ¹ and G ² is a linked specific binding member.

In certain embodiments of the systems, the composition further includes a second specific binding member that is support bound and specifically binds the target analyte. In some cases, the support includes a magnetic particle. As such, in certain instances, the system may also include a controllable external paramagnetic field configured for application to an assay region of the flow channel.

The sample may include a cell. In some instances, the sample is a cell-containing biological sample. In some instances, the sample includes a labelled specific binding member specifically bound to a target cell. In certain instances, the target analyte that is specifically bound by the specific binding member is a cell surface marker of the cell. In certain cases, the cell surface marker is selected from the group consisting of a cell receptor and a cell surface antigen.

In certain aspects, the system may also include a light source configured to direct light to an assay region of the flow channel or sample field of view. The system may include a detector configured to receive a signal from an assay region of the flow channel or a sample field of view, wherein the signal is provided by the fluorescent composition. Optionally further, the sample analysis system may include one or more additional detectors and/or light sources for the detection of one or more additional signals.

In certain aspects, the system may further include computer-based systems configured to detect the presence of the fluorescent signal. A "computer-based system" refers to the hardware means, software means, and data storage means used to analyze the information of the present invention. The minimum hardware of the computer-based systems of the present invention includes a central processing unit (CPU), input means, output means, and data storage means. A skilled artisan can readily appreciate that any one of the currently available computer-based system are suitable for use in the subject systems. The data storage means may include any manufacture including a recording of the present information as described above, or a memory access means that can access such a manufacture.

To "record" data, programming or other information on a computer readable medium refers to a process for storing information, using any such methods as known in the art. Any convenient data storage structure may be chosen, based on the means used to access the stored information. A variety of data processor programs and formats can be used for storage, e.g., word processing text file, database format, etc.

A "processor" references any hardware and/or software combination that will perform the functions required of it. For example, any processor herein may be a programmable digital microprocessor such as available in the form of an electronic controller, mainframe, server or personal computer (desktop or portable). Where the processor is programmable, suitable programming can be communicated from a remote location to the processor, or previously saved in a computer program product (such as a portable or fixed computer readable storage medium, whether magnetic, optical or solid state device based). For example, a magnetic medium or optical disk may carry the programming, and can be read by a suitable reader communicating with each processor at its corresponding station.

In addition to the sensor device and signal processing module, e.g., as described above, systems of the invention may include a number of additional components, such as data output devices, e.g., monitors and/or speakers, data input devices, e.g., interface ports, keyboards, etc., fluid handling components, power sources, etc.

In certain aspects, the system includes a flow cytometer. Suitable flow cytometer systems and methods for analyzing samples include, but are not limited to those described in Ormerod (ed.), Flow Cytometry: A Practical Approach, Oxford Univ. Press (1997); Jaroszeski et al. (eds.), Flow Cytometry Protocols, Methods in Molecular Biology No. 91 , Humana Press (1997); Practical Flow Cytometry, 3rd ed., Wiley-Liss (1995); Virgo, et al. (2012) Ann Clin Biochem. Jan;49(pt 1 ):17-28; Linden, et. al., Semin Throm Hemost. 2004 Oct;30(5):502-1 1 ; Alison, et al. J Pathol, 2010 Dec; 222(4) :335-344; and Herbig, et al. (2007) Crit Rev Ther Drug Carrier Syst. 24(3):203-255; the disclosures of which are incorporated herein by reference. In certain instances, flow cytometry systems of interest include BD Biosciences FACSCanto™ II flow cytometer, BD Accuri™ flow cytometer, BD Biosciences FACSCelesta™ flow cytometer,

BD Biosciences FACSLyric™ flow cytometer, BD Biosciences FACSVerse™ flow cytometer, BD Biosciences FACSymphony™ flow cytometer BD Biosciences LSRFortessa™ flow cytometer, BD Biosciences LSRFortess™ X-20 flow cytometer and BD Biosciences FACSCalibur™ cell sorter, a BD Biosciences FACSCount™ cell sorter, BD Biosciences FACSLyric™ cell sorter and BD Biosciences Via™ cell sorter BD Biosciences Influx™ cell sorter, BD Biosciences Jazz™ cell sorter, BD Biosciences Aria™ cell sorters and BD Biosciences FACSMelody™ cell sorter, or the like. In certain embodiments, the subject systems are flow cytometer systems which incorporate one or more components of the flow cytometers described in U.S. Patent No.

10,006,852; 9,952,076; 9,933,341 ; 9,784,661 ; 9,726,527; 9,453,789; 9,200,334; 9,097,640; 9,095,494; 9,092,034; 8,975,595; 8,753,573; 8,233,146; 8,140,300; 7,544,326; 7,201 ,875; 7,129,505; 6,821 ,740; 6,813,017; 6,809,804; 6,372,506; 5,700,692; 5,643,796; 5,627,040; 5,620,842; 5,602,039; the disclosure of which are herein incorporated by reference in their entirety.

In certain instances, the subject systems are flow cytometry systems configured for imaging particles in a flow stream by fluorescence imaging using radiofrequency tagged emission (FIRE), such as those described in Diebold, et al. Nature Photonics Vol. 7(10); 806- 810 (2013) as well as described in U.S. Patent Nos. 9,423,353; 9,784,661 and 10,006,852 and U.S. Patent Publication Nos. 2017/0133857 and 2017/0350803, the disclosures of which are herein incorporated by reference.

Other systems may find use in practicing the subject methods. In certain aspects, the system may be a fluorimeter or microscope loaded with a sample having a fluorescent composition of any of the embodiments discussed herein. The fluorimeter or microscope may include a light source configured to direct light to the assay region of the flow channel or sample field of view. The fluorimeter or microscope may also include a detector configured to receive a signal from an assay region of the flow channel or field of view, wherein the signal is provided by the fluorescent composition.

KITS

Aspects of the invention further include kits for use in practicing the subject methods and compositions. The compositions of the invention can be included as reagents in kits either as starting materials or provided for use in, for example, the methodologies described above.

A kit can include a polymeric dye including a water solvated light harvesting

multichromophore (e.g., as described herein) and a container. Any convenient containers can be utilized, such as tubes, bottles, or wells in a multi-well strip or plate, a box, a bag, an insulated container, and the like. The subject kits can further include one or more components selected from a polymeric tandem dye, a fluorophore, a specific binding member, a specific binding member conjugate, a support bound specific binding member, a cell, a support, a biocompatible aqueous elution buffer, and instructions for use. In some embodiments of the kit, the multichromophore is covalently linked to a specific binding member. In some instances, the specific binding member is an antibody. In certain instances, the specific binding member is an antibody fragment or binding derivative thereof. In certain cases, the antibody fragment or binding derivative thereof is selected from the group consisting of a Fab fragment, a F(ab')2 fragment, a scFv, a diabody and a triabody.

In certain embodiments, the kit finds use in evaluating a sample for the presence of a target analyte, such as an intracellular target. As such, in some instances, the kit includes one or more components suitable for lysing cells. The one or more additional components of the kit may be provided in separate containers (e.g., separate tubes, bottles, or wells in a multi-well strip or plate).

In certain aspects, the kit further includes reagents for performing a flow cytometric assay. Reagents of interest include, but are not limited to, buffers for reconstitution and dilution, buffers for contacting a cell sample the multichromophore, wash buffers, control cells, control beads, fluorescent beads for flow cytometer calibration and combinations thereof. The kit may also include one or more cell fixing reagents such as paraformaldehyde, glutaraldehyde, methanol, acetone, formalin, or any combinations or buffers thereof. Further, the kit may include a cell permeabilizing reagent, such as methanol, acetone or a detergent (e.g., triton, NP- 40, saponin, tween 20, digitonin, leucoperm, or any combinations or buffers thereof. Other protein transport inhibitors, cell fixing reagents and cell permeabilizing reagents familiar to the skilled artisan are within the scope of the subject kits.

The compositions of the kit may be provided in a liquid composition, such as any suitable buffer. Alternatively, the compositions of the kit may be provided in a dry composition (e.g., may be lyophilized), and the kit may optionally include one or more buffers for

reconstituting the dry composition. In certain aspects, the kit may include aliquots of the compositions provided in separate containers (e.g., separate tubes, bottles, or wells in a multi well strip or plate).

In addition, one or more components may be combined into a single container, e.g., a glass or plastic vial, tube or bottle. In certain instances, the kit may further include a container (e.g., such as a box, a bag, an insulated container, a bottle, tube, etc.) in which all of the components (and their separate containers) are present. The kit may further include packaging that is separate from or attached to the kit container and upon which is printed information about the kit, the components of the and/or instructions for use of the kit.

In addition to the above components, the subject kits may further include instructions for practicing the subject methods. These instructions may be present in the subject kits in a variety of forms, one or more of which may be present in the kit. One form in which these instructions may be present is as printed information on a suitable medium or substrate, e.g., a piece or pieces of paper on which the information is printed, in the packaging of the kit, in a package insert, etc. Yet another means would be a computer readable medium, e.g., diskette, CD, DVD, portable flash drive, etc., on which the information has been recorded. Yet another means that may be present is a website address which may be used via the Internet to access the information at a removed site. Any convenient means may be present in the kits.

UTILITY

The polymeric dyes, compositions, methods and systems as described herein may find use in a variety of applications, including diagnostic and research applications, in which the labelling, detection and/or analysis of a target of interest is desirable. Such applications include methodologies such as cytometry, microscopy, immunoassays (e.g. competitive or non competitive), assessment of a free analyte, assessment of receptor bound ligand, and so forth. The compositions, system and methods described herein may be useful in analysis of any of a number of samples, including but not limited to, biological fluids, cell culture samples, and tissue samples. In certain aspects, the compositions, system and methods described herein may find use in methods where analytes are detected in a sample, if present, using fluorescent labels, such as in fluorescent activated cell sorting or analysis, immunoassays, immunostaining, and the like. In certain instances, the compositions and methods find use in applications where the evaluation of a sample for the presence of a target analyte is of interest.

In some cases, the methods and compositions find use in any assay format where the detection and/or analysis of a target from a sample is of interest, including but not limited to, flow cytometry, fluorescence microscopy, in-situ hybridization, enzyme-linked immunosorbent assays (ELISAs), western blot analysis, magnetic cell separation assays and fluorochrome purification chromatography. In certain instances, the methods and compositions find use in any application where the fluorescent labelling of a target molecule is of interest. The subject compositions may be adapted for use in any convenient applications where pairs of specific binding members find use, such as biotin-streptavidin and hapten-anti-hapten antibody.

EXAMPLES

The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use embodiments of the present disclosure, and are not intended to limit the scope of what the inventors regard as their invention nor are they intended to represent that the experiments below are all or the only experiments performed. Efforts have been made to ensure accuracy with respect to numbers used (e.g. amounts, temperature, etc.) but some experimental errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Centigrade, and pressure is at or near atmospheric.

While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present disclosure. All such modifications are intended to be within the scope of the claims appended hereto.

Example 1 : Preparation of multichromophores and polymeric tandem dves

The synthesis of the subject multichromophores can be achieved utilizing ring opening metathesis polymerization (ROMP) methodology.

Many general references providing commonly known chemical synthetic schemes and conditions useful for synthesizing the disclosed starting materials are also available (see, e.g., Smith and March, March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, Fifth Edition, Wiley-lnterscience, 2001 ; or Vogel, A Textbook of Practical Organic Chemistry, Including Qualitative Organic Analysis, Fourth Edition, New York: Longman, 1978).

Synthesis of exemplary multichromophores

1 ) Polymer 1

Polymer 1 was synthesized according to Scheme 4:

1) Solvent, catalyst

Scheme 4: Synthesis of Polymer 1

where: Z ¹ = BODIPY-OH; n and m are each an integer from 1 to 25 With reference to scheme 4, a first norbornene monomer unit of structure 1 A is contacted with a second norbornene monomer unit of structure 1 B in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 1 .

2) Polymer 2

Polymer 2 was synthesized according to Scheme 5:

Scheme 5: Synthesis of Polymer 2

where: Z ¹ = BODIPY-OH; n and m are each an integer from 1 to 25

With reference to scheme 5, a first norbornene monomer unit of structure 2A is contacted with a second norbornene monomer unit of structure 1 B in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 2.

3) Polymer 3

Polymer 3 was synthesized according to Scheme 6:

Scheme 6: Synthesis of Polymer 3

where: Z ¹ = BODIPY-OH; n is an integer from 1 to 25 With reference to scheme 6, a norbornene monomer unit of structure 3A is treated with a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 3.

4) Polymer 4

Polymer 4 was synthesized according to Scheme 7:

Scheme 7: Synthesis of Polymer 4

where: Z ¹ = BODIPY-OH; n and m are each an integer from 1 to 25

With reference to scheme 7, a first norbornene monomer unit of structure 4A is contacted with a second norbornene monomer unit of structure 1 B in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 4.

5) Polymer 5

Polymer 5 was synthesized according to Scheme 8:

Scheme 8: Synthesis of Polymer 5

where: Z ¹ = Rhodamine B; n and m are each an integer from 1 to 25 With reference to scheme 8, a first norbornene monomer unit of structure 5A is contacted with a second norbornene monomer unit of structure 1 B in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 5.

6) Polymer 6

Polymer 6 was synthesized according to Scheme 9:

Scheme 9: Synthesis of Polymer 6

where: Z ¹ = Rhodamine B; n and m are each an integer from 1 to 25

With reference to scheme 9, a first norbornene monomer unit of structure 6A is contacted with a second norbornene monomer unit of structure 1 B in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 6.

7) Polymer 7

Polymer 7 was synthesized according to Scheme 10:

Scheme 10: Synthesis of Polymer 7

where: Z ¹ = Rhodamine B; n is an integer from 1 to 25

With reference to scheme 10, a norbornene monomer unit of structure 7A is treated with a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide Polymer 7. Synthesis of exemplary polymeric tandem dyes

8) Polymer 8

Polymer 8 was synthesized according to Scheme 1 1 :

Scheme 1 1: Synthesis of Polymer 8

where: Z ¹ = BODIPY-OH; Z ² is Rhodamine B; n and m are each an integer from 1 to 25

With reference to scheme 1 1 , a first norbornene monomer unit of structure 4A is contacted with a second norbornene monomer unit of structure 8A in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide the polymeric tandem dye, Polymer 8.

9) Polymer 9

Polymer 9 was synthesized according to Scheme 12:

Scheme 12: Synthesis of Polymer 9

where: Z ¹ = BODIPY-OH; Z ³ is Rhodamine 6G, perylene diimide or cyanine 3; n and m are each an integer from 1 to 25 With reference to scheme 1 1 , a first norbornene monomer unit of structure 4A is contacted with a second norbornene monomer unit of structure 9A in the presence a ruthenium catalyst (e.g., Grubbs catalyst) and tert-butyl N-allyl carbamate at a temperature from -20 °C to 220 °C and a pressure of from 0.01 atm to 10 atm, to provide the polymeric tandem dye, Polymer 9.

Notwithstanding the appended claims, the disclosure is also defined by the following clauses:

1. A water-soluble polymeric multichromophore comprising:

a polymeric backbone comprising norbornene repeat units;

a pendant water solubilizing group (WSG) component; and

a pendant chromophore component,

wherein each water solubilizing group and each chromophore group are linked to a norbornene repeat unit of the polymeric backbone.

2. The polymeric multichromophore according to clause 1 , wherein the pendant chromophore component comprises a donor chromophore.

3. The polymeric multichromophore according to any one of clauses 1 -2, wherein the pendant chromophore component comprises an acceptor chromophore.

4. The polymeric multichromophore according to any one of clauses 1 -3, wherein the pendant chromophore component comprises a donor chromophore and an acceptor chromophore.

5. The polymeric multichromophore according to clause 4, wherein the donor chromophore and the acceptor chromophore are positioned in energy-transferring proximity to each other.

6. The polymeric multichromophore according to any one of clauses 1 -5, wherein the pendant chromophore component comprises a fused tricyclic aryl group.

7. The polymeric multichromophore according to any one of clauses 1 -5, wherein the pendant chromophore component comprises a heteroaryl group.

8. The polymeric multichromophore according to any one of clauses 1 -7, wherein the pendant chromophore component comprises a chromophore selected from the group consisting of boron-dipyrromethene (BODIPY), Rhodamine, perylene diimide (PDI), and Cyanine.

9. The polymeric multichromophore according to any one of clauses 1 -8, wherein the pendant chromophore component is linked to the norbornene repeat unit through a hydroxyl or a carboxylate functional group.

10. The polymeric multichromophore according to any one of clauses 1 -9, wherein the pendant water solubilizing group component comprises poly(ethylene) glycol (PEG).

1 1 . The polymeric multichromophore according to any one of clauses 1 -10, wherein the polymeric backbone comprises a segment having a formula (NB-I): wherein:

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ¹ , R ² , R ³ and R ⁴ are each independently selected from hydrogen, a water solubilizing group and a chromophore, wherein at least one of R ¹ , R ² , R ³ and R ⁴ is a chromophore and at least one of R ¹ , R ² , R ³ and R ⁴ is a water solubilizing group;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore; and

n is an integer from 1 to 1000; and

m is an integer from 0 to 1000.

12. The polymeric multichromophore according to clause 1 1 , wherein the polymeric backbone comprises a segment having a formula of:

d) formula (NB-IC):

e) formula (NB-IE):

f) formula (NB-IF):

g) formula (NB-IG):

or

h) formula (NB-IH):

wherein:

R ⁷ , R ⁸ , and R ^{1 1} are each independently a water solubilizing group; R ⁹ , R ¹⁰ , and R ¹² are each independently a chromophore. 13. The polymeric multichromophore according to any one of clauses 1 -10, wherein the polymeric backbone comprises a segment having a formula (NB-II):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ¹ , R ² , R ³ and R ⁴ are each independently selected from hydrogen, a water solubilizing group and a chromophore, wherein at least one of R ¹ , R ² , R ³ and R ⁴ is a chromophore and at least one of R ¹ , R ² , R ³ and R ⁴ is a water solubilizing group;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

m is an integer from 0 to 1000; and

r is an integer from 0 to 10.

14. The polymeric multichromophore according to clause 13, wherein the polymeric backbone comprises a segment having a formula of: a) formula (NB-IIA):

b) formula (NB-IIB): c) formula (NB-IIC):

d) formula (NB-IID): e) formula (NB-IIE): f) formula (NB-IIF): g) formula (NB-IIG):

h) formula (NB-IIH):

wherein:

R ⁷ , R ⁸ , and R ¹¹ are each independently a water solubilizing group;

R ⁹ , R ¹⁰ , and R ¹² are each independently a chromophore.

15. The polymeric multichromophore according to any one of clauses 1 -14, wherein the water solubilizing group (WSG) component comprises a water solubilizing group selected from the group consisting of:

wherein:

T ⁵ is a bond or a linker;

each T ⁶ is a linker;

R ³⁰ and R are independently H, alkyl or substituted alkyl; and

each s is an integer from 1 to 50.

16. The polymeric multichromophore according to any one of clauses 1 1 -15, wherein L ¹ , L ² , L ³ and L ⁴ are each independently a linker selected from alkyl, substituted alkyl, acyl, substituted acyl, alkoxy, substituted alkoxy, amino, substituted amino, hydroxyl, an alkyl-amido-alkyl, or a PEG moiety.

17. The polymeric multichromophore according to any one of clause 1 -10, wherein the polymeric backbone comprises a segment having a formula of:

a) formula (NB-IIIA):

b) formula (NB-IIIB):

c) formula (NB-IIIC):

or

d) formula (NB-IIID):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ⁵ , L ⁶ and L ⁷ are each independently a bond or a linker;

Z ¹ , Z ² and Z ³ are each independently selected from a chromophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n and m are each independently an integer from 1 to 1000;

r is an integer from 0 to 10; and

S ¹ -S ³ are each independently an integer from 1 to 50.

18. The polymeric multichromophore according to clause 17, wherein any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formulae -C(O)OZ ⁴ or -(CR ³¹ ) _t OC(O)Z ⁴ ,

wherein:

Z ⁴ is a chromophore;

R ³¹ is selected from H, alkyl, substituted alkyl; and

t is an integer from 1 to 10.

19. The polymeric multichromophore according to clause 18, wherein any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formula -C(O)OZ ⁴ .

20. The polymeric multichromophore according to clause 18, wherein any of -L ⁵ -Z ² , -L ⁶ -Z ¹ and -L ⁷ -Z ³ are described by the formula -CH ₂ O(O)Z ⁴ .

21 . The polymeric multichromophore according to any one of clauses 13-20, wherein G ¹ is a terminal group comprising a group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne.

22. The polymeric multichromophore according to any one of clauses 1 -21 , wherein the pendant chromophore component comprises BODIPY or a derivative thereof.

23. The polymeric multichromophore according to clause 22, wherein the BODIPY is of formula (BD-I):

wherein:

R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group; and

each R ¹³ is selected from the group consisting of F, OH, H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkynyl and substituted alkynyl and a water solubilizing group;

wherein one of R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ is linked to the norbornene repeat unit of the polymeric backbone.

24. The multichromophore according to clause 23, wherein the BODIPY is of formula (BD- II):

wherein :

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ^17a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

p is an integer from 0 to 4.

25. The polymeric multichromophore according to any one of clauses 1 -21 , wherein the pendant chromophore component comprises a Rhodamine or a derivative thereof. 26. The polymeric multichromophore according to clause 25, wherein the Rhodamine is of formula (RH-I):

wherein:

each R ²¹ is selected from hydrogen, alkyl and substituted alkyl;

each R ²² is selected from hydrogen, alkyl and substituted alkyl;

R ²³ , R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ and R ²⁸ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group;

R ²⁹ is selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

q is an integer from 0 to 5;

wherein one of R ²³ R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ , R ²⁸ or R ²⁹ is linked to a norbornene repeat unit of the polymeric backbone.

27. The polymeric multichromophore according to clause 26, wherein the Rhodamine is of formula (RH-II):

wherein:

is a point of linkage to a norbornene repeat unit of the polymeric backbone; each R ^29a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

r is an integer from 0 to 4.

28. A polymeric tandem dye comprising:

a light harvesting chromophore comprising:

a polymeric backbone comprising norbornene repeat units;

a pendant water solubilizing (WSG) component; and

a pendant donor chromophore component,

wherein each water solubilizing group and each chromophore group are linked to a norbornene repeat unit of the polymeric backbone; and

an energy acceptor comprising a pendant acceptor fluorophore covalently bonded to the polymeric backbone in energy-receiving proximity to the donor chromophore.

29. The polymeric tandem dye according to clause 28, wherein the pendant donor chromophore comprises a fused tricyclic aryl group or a heteroaryl group.

30. The polymeric tandem dye according to clause 28, wherein the pendant donor chromophore is selected from the group consisting of BODIPY, Rhodamine, perylene diimide (PDI), and Cyanine.

31 . The polymeric tandem dye according to any one of clauses 28-30, wherein the pendant donor chromophore is linked to a norbornene repeat unit through a hydroxyl or a carboxylate functional group.

32. The polymeric tandem dye according to any one of clauses 28-31 , wherein the pendant water solubilizing group comprises poly(ethylene) glycol (PEG).

33. The polymeric tandem dye according to any one of clauses 28-32, wherein the polymeric backbone comprises a segment having a formula (NB-IV):

wherein:

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ³² and R ³⁴ are each independently a water solubilizing group;

R ³³ is a donor chromophore or an acceptor fluorophore; R ³⁵ is a donor chromophore or an acceptor fluorophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore; and

n and m are each independently an integer from 1 to 1000.

34. The polymeric tandem dye according to any one of clauses 28-33, wherein the polymeric backbone comprises a segment of formula (NB-V):

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ¹ , L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ³² and R ³⁴ are each independently a water solubilizing group;

R ³³ is a donor chromophore or an acceptor fluorophore;

R ³⁵ is a donor chromophore or an acceptor fluorophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

m is an integer from 0 to 1000; and

r is an integer from 0 to 10.

35. The polymeric tandem dye according to any one of clauses 28-34, wherein the water solubilizing group (WSG) component comprises a water solubilizing group selected from the group consisting of:

wherein:

T ⁵ is a bond or a linker;

each T ⁶ is a linker;

R ³⁰ and R are independently H, alkyl or substituted alkyl; and

each s is an integer from 1 to 50.

36. The polymeric tandem dye according to any one of clauses 34-35, wherein the polymeric backbone comprises a segment of formula (NB-VA):

wherein:

S ¹ and S ³ are each independently an integer from 1 to 50. 37. The polymeric tandem dye according to any one of clauses 34-36, wherein Y ¹ and Y ² are each CH ₂ .

38. The polymeric tandem dye according to any one of clauses 34-37, wherein L ¹ , L ² , L ³ and L ⁴ are each independently a linker selected from alkyl, substituted alkyl, acyl, substituted acyl, alkoxy, substituted alkoxy, amino, substituted amino, hydroxyl, an alkyl-amido-alkyl, or a PEG moiety.

39. The polymeric tandem dye according to clause 38, wherein -L ² -R ³³ , and -L ⁴ -R ³⁴ are each independently described by the formulae -C(O)OR ³⁶ and -(CR ³¹ ) _t OC(O)R ³⁶ ,

wherein:

R ³⁶ is selected from a donor chromophore and an acceptor fluorophore;

R ³¹ is selected from H, alkyl, substituted alkyl; and

t is an integer from 1 to 10.

40. The polymeric tandem dye according to clause 39, wherein each of -L ² -R ³³ , and -L ⁴ -R ³⁴ are described by the formula -C(O)OR ³⁶ .

41 . The polymeric tandem dye according to clause 39, wherein

-L ² -R ³³ is of the formula -C(O)OR ³⁶ , wherein R ³⁶ is a donor chromophore; and

-L ⁴ -R ³⁴ is of the formula -CH ₂ OC(O)R ³⁶ , wherein R ³⁶ is an acceptor fluorophore.

42. The polymeric tandem dye according to any one of clauses 34-41 , wherein G ¹ is a terminal group comprising a group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne.

43. The polymeric tandem dye according to any one of clauses 28-42, wherein the pendant donor chromophore comprises BODIPY or a derivative thereof.

44. The polymeric tandem dye according to clause 43, wherein the BODIPY is of the formula (BD-I):

wherein:

R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group; and

each R ¹³ is selected from the group consisting of F, OH, H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, alkynyl and substituted alkynyl and a water solubilizing group;

wherein one of R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ , R ¹⁸ , R ¹⁹ and R ²⁰ is linked to the norbornene repeat unit of the polymeric backbone.

45. The polymeric tandem dye according to clause 44, wherein the BODIPY is of formula (BD-II):

wherein:

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ^17a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

p is an integer from 0 to 4.

46. The polymeric tandem dye according to any one of clauses 28-45, wherein the pendant donor chromophore comprises a Rhodamine or a derivative thereof.

47. The polymeric tandem dye according to clause 46, wherein the Rhodamine is of formula (RH-I):

wherein:

each R ²¹ is selected from hydrogen, alkyl and substituted alkyl;

each R ²² is selected from hydrogen, alkyl and substituted alkyl;

R ²³ , R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ and R ²⁸ are each independently selected from H, alkyl, substituted alkyl, hydroxyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl and a water solubilizing group;

R ²⁹ is selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

q is an integer from 0 to 5;

wherein one of R ²³ R ²⁴ , R ²⁵ , R ²⁶ , R ²⁷ , R ²⁸ or R ²⁹ is linked to a norbornene repeat unit of the polymeric backbone.

48. The polymeric tandem dye according to clause 47, wherein the Rhodamine is of formula (RH-II):

wherein:

^* is a point of linkage to a norbornene repeat unit of the polymeric backbone;

each R ^29a is an optional substituent selected from halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carboxyl, acyl, substituted acyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; and

r is an integer from 0 to 4.

49. The polymeric tandem dye according to any one of clauses 28-48, wherein the pendant acceptor fluorophore is a small molecule fluorophore.

50. The polymeric tandem dye according to any one of clauses 28-49, wherein the pendant acceptor fluorophore is selected from the group consisting of a cyanine dye, a rhodamine dye, a perylene diimide dye, a xanthene dye, a coumarin dye, a polymethine, a pyrene, a

dipyrromethene borondifluoride, a napthalimide, a thiazine dye and an acridine dye. 51 . The polymeric tandem dye according to clause 50, wherein the pendant acceptor fluorophore is a dye selected from the group consisting of:

wherein:

each X are independently selected from hydrogen, a lone pair of electrons, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, hydroxyl and halogen; and

each Y are independently selected from hydrogen, a lone pair of electrons, alkyl and substituted alkyl (e.g., alkyl chain terminating in a NHS-ester or maleimide moiety).

52. A method comprising contacting a first norbornene monomer comprising a pendant chromophore component with a second norbornene monomer comprising a pendant water solubilizing group component to generate a polymeric backbone comprising a norbornene repeat unit, a pendant water solubilizing group (WSG) component and a pendant chromophore component.

53. The method according to clause 52, wherein the first norbornene monomer further comprises a water solubilizing group component.

54. The method according to any one of clauses 52-53, wherein the second norbornene monomer further comprises a pendant chromophore component.

55. The method according to any one of clauses 52-54, wherein the second monomer further comprises an acceptor fluorophore group and the contacting generates a polymeric backbone comprising a pendant water solubilizing group (WSGs) component, a pendant chromophore component and a pendant acceptor fluorophore group. 56. The method according to any one of clauses 52-55, wherein the first norbornene monomer is contacted with the second norbornene monomer in the presence of a catalyst and an alkene.

57. The method according to clause 56, wherein the catalyst is a ruthenium catalyst.

58. The method according to any one of clauses 56-57, wherein the alkene is of the formula CH ₂ =CH(CH ₂ ) _u G ¹ ,

wherein:

G ¹ is selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member; and

u is an integer from 0 to 10.

59. The method according to any one of clauses 52-58, wherein the polymeric backbone is of the formula:

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

U, L ² , L ³ and L ⁴ are each independently a bond or a linker;

R ¹ , R ² , R ³ and R ⁴ are each independently selected from hydrogen, a water solubilizing group and a chromophore, wherein at least one of R ¹ , R ² , R ³ and R ⁴ is a chromophore and at least one of R ¹ , R ² , R ³ and R ⁴ is a water solubilizing group;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

m is an integer from 0 to 1000; and

r is an integer from 0 to 10.

60. The method according to any one of clauses 52-59, wherein the norbornene polymer is of the formula:

wherein:

G ¹ is a selected from a terminal group, a polymer segment, a donor chromophore group, a linker and a linked specific binding member;

L ¹ , L ² , L ³ and L ⁴ are each independently an optional linker;

R ³² and R ³⁴ are selected from a water solubilizing group;

R ³³ is selected from a donor chromophore;

R ³⁵ is selected from an acceptor fluorophore;

Y ¹ and Y ² are each independently selected from CR ⁵ and O;

R ⁵ is selected from hydrogen halogen, hydroxyl, cyano, nitro, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, substituted acyl, carboxyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, wherein R ⁵ is optionally substituted with a water solubilizing group or a chromophore;

n is an integer from 1 to 1000;

m is an integer from 0 to 1000; and

r is an integer from 0 to 10.

61 . The method according to any one of clauses 59-60, wherein G ¹ is a terminal group comprising a group selected from an amine, carboxylic acid, isocyanate, isothiocyanate, azide, thiol, hydrazide, disulfide, maleimide, NHS ester, acrylate, vinyl sulfone, and an alkyne.

62. The method according to any one of 52-60, wherein the first monomer and second monomer are contacted at a temperature from -20 °C to 220 °C.

63. The method according to any one of clauses 52-62, wherein the first monomer and second monomer are contacted at a pressure from 0.01 atmospheres to 10 atmospheres.

64. The method according to any one of clauses 52-63, wherein the first monomer and second monomer are contacted in a solvent selected from dichloromethane, chloroform, dimethylacetamide, toluene, xylene, benzene, dimethyl formamide, acetonitrile and dimethyl sulfoxide.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.

Accordingly, the preceding merely illustrates the principles of the invention. It will be appreciated that those skilled in the art will be able to devise various arrangements which, although not explicitly described or shown herein, embody the principles of the invention and are included within its spirit and scope. Furthermore, all examples and conditional language recited herein are principally intended to aid the reader in understanding the principles of the invention and the concepts contributed by the inventors to furthering the art, and are to be construed as being without limitation to such specifically recited examples and conditions.

Moreover, all statements herein reciting principles, aspects, and embodiments of the invention as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents and equivalents developed in the future, i.e., any elements developed that perform the same function, regardless of structure. Moreover, nothing disclosed herein is intended to be dedicated to the public regardless of whether such disclosure is explicitly recited in the claims.

The scope of the present invention, therefore, is not intended to be limited to the exemplary embodiments shown and described herein. Rather, the scope and spirit of present invention is embodied by the appended claims. In the claims, 35 U.S.C. §1 12(f) or 35 U.S.C. §1 12(6) is expressly defined as being invoked for a limitation in the claim only when the exact phrase "means for" or the exact phrase "step for" is recited at the beginning of such limitation in the claim; if such exact phrase is not used in a limitation in the claim, then 35 U.S.C. § 1 12 (f) or 35 U.S.C. §1 12(6) is not invoked.