Title:
【発明の名称】ネスチンプロモーターの制御下で蛍光タンパク質を発現するトランスジェニックマウス
Document Type and Number:
Japanese Patent JP2003514550
Kind Code:
A
Abstract:
Non-human transgenic mammals are produced which have, incorporated in their genome, DNA which includes a regulatory sequence of a mammalian nestin gene, operably linked to a gene coding for a marker/reporter protein. The regulatory sequence can include a promoter and a sequence present in the second intron of the mammalian nestin gene. Preferably, the marker/reporter protein is a fluorescent protein, for example a green fluorescent protein, modified for enhanced fluorescence. Multipotent and, in particular, neural stem and progenitor cell populations are observed in the organs of the non-transgenic mammal or progeny thereof. Multipotent stem and progenitor cells are isolated directly from the non-human transgenic mammal, progeny or embryo thereof, for example by FACS, without culture passages.
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Inventors:
Enikolopov, Grigori, N.
John Mignon
John Mignon
Application Number:
JP2001538971A
Publication Date:
April 22, 2003
Filing Date:
November 14, 2000
Export Citation:
Assignee:
Cold Spring Harbor Laboratory
International Classes:
A01K67/027; C07K14/47; C12N5/10; C12N15/09; C12N15/85; C12Q1/02; (IPC1-7): C12N15/09; A01K67/027; C12N5/10; C12Q1/02
Attorney, Agent or Firm:
Yoshinori Hosoda