発現ベクター、異種遺伝子生成物の製造方法およびこのような生成物を高濃度で製造する組換え細胞の選別方法 - Boehringer Ingelheim Pharma Gesellshaft Mitt Beschlenktel Haftung Und Company Comandito Gesellshaft

Title:

発現ベクター、異種遺伝子生成物の製造方法およびこのような生成物を高濃度で製造する組換え細胞の選別方法

Document Type and Number:

Japanese Patent JP2006507829

Kind Code:

A

Abstract:

Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, said expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.

More Like This:

WO/2008/007571	DETECTION METHOD FOR NUCLEIC ACID
WO/2003/009100	METHODS, APPARATUSES, AND COMPUTER PROGRAMS FOR VERIFYING THE INTEGRITY OF A PROBE
JP2002528381	INDUSTRIAL APPLICABILITY: Cell sensitization to a compound utilizing a lipid-mediated gene and compound introduction.

Inventors:

Enenkel Barbara
Feeder juergen
Otto Ralph
Gramatikos Stefanos

Application Number:

JP2004556182A

Publication Date:

March 09, 2006

Filing Date:

November 25, 2003

Export Citation:

Click for automatic bibliography generation Help

Assignee:

Boehringer Ingelheim Pharma Gesellshaft Mitt Beschlenktel Haftung Und Company Comandito Gesellshaft

International Classes:

C12N15/09; C12N5/10; C12N15/65; C12N15/69; C12P21/02; C12Q1/04

Attorney, Agent or Firm:

Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama

Previous Patent: 植物原料由来イノシトールの精製

Next Patent: ソース類用増粘組成物