Title:
L-ラムノース誘導性発現系
Document Type and Number:
Japanese Patent JP2006507831
Kind Code:
A
Abstract:
The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in a host cell and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where the promoter is heterologous with regard to the nucleic acid sequence, is introduced into the host cell and the expression of he nucleic acid sequence is induced by addition of L-rhamnose, wherein the prokaryotic host cell is at least deficient with regard to an L-rhamnose isomerase.
Inventors:
Maria, Kessler
Zelinsky, Thomas
Bernhard, Hauer
Zelinsky, Thomas
Bernhard, Hauer
Application Number:
JP2004556204A
Publication Date:
March 09, 2006
Filing Date:
November 27, 2003
Export Citation:
Assignee:
BASF Aktiengesellschaft
International Classes:
C12N15/09; C12N1/21; C12N9/00; C12N15/63; C12N15/70; C12P21/02
Domestic Patent References:
| JP2003527095A | 2003-09-16 | |||
| JP2003527095A | 2003-09-16 |
Other References:
JPN6009051366, Ｂｉｏｔｅｃｈｎｏｌ Ｂｉｏｅｎｇ， ｖｏｌ．７３， ｐ．９５−１０３ （２００１）
JPN6009051367, Ｊ Ｂａｃｔｅｒｉｏｌ， ｖｏｌ．１５８， ｐ．６０３−６０８ （１９８４）
JPN6009051368, Ｂｉｏｓｐｅｃｔｒｕｍ， ｖｏｌ．６， ｐ．３３−３６ （２０００）
JPN6009051553, Ｊ Ｍｏｌ Ｂｉｏｌ， ｖｏｌ．２１１， ｐ．１−４ （１９９０）
JPN6009051367, Ｊ Ｂａｃｔｅｒｉｏｌ， ｖｏｌ．１５８， ｐ．６０３−６０８ （１９８４）
JPN6009051368, Ｂｉｏｓｐｅｃｔｒｕｍ， ｖｏｌ．６， ｐ．３３−３６ （２０００）
JPN6009051553, Ｊ Ｍｏｌ Ｂｉｏｌ， ｖｏｌ．２１１， ｐ．１−４ （１９９０）
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita