Arenicolamarinaの細胞外ヘモグロビン分子の解離方法及び該分子を形成するタンパク質鎖の特徴付け及び該タンパク質鎖をコードするヌクレオチド配列 - CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Title:

Arenicolamarinaの細胞外ヘモグロビン分子の解離方法及び該分子を形成するタンパク質鎖の特徴付け及び該タンパク質鎖をコードするヌクレオチド配列

Document Type and Number:

Japanese Patent JP2007508024

Kind Code:

A

Abstract:

Dissociating molecules of the extracellular hemoglobin (I) of Annelids, particularly Arenicola marina, to produce the 10 protein chains (II) that make up (I), comprises treating (I) with at least one dissociating agent (III). (III) is particularly a mixture of dithiothreitol; tris(2-carboxyethyl)phosphine or 2-mercaptoethanol with a dissociation buffer. Independent claims are also included for: (1) preparing a pair of primers (P) from (II); (2) pair of (P) produced by method (1); (3) preparing nucleic acid sequences (NA) that encode (I), using the primers of (1); (4) proteins encoded by NA; and (5) NA produced by method (3) or encoding the proteins of (4).

More Like This:

JP6122062	Inclusion and revelation by which visitor nucleic acid arrangement was targeted
WO/1996/026278	HUMAN DNase I VARIANTS
JP2009207449	METHOD FOR PREPARING PROTEIN AGGREGATE CONTAINING LAMININ COILED COIL(LCC) DOMAIN HETEROMULTIMER

Inventors:

Colander, frank
Chabas, Christine
Ruthro, Morgan
Bailly, Xavier

Application Number:

JP2006534789A

Publication Date:

April 05, 2007

Filing Date:

October 13, 2004

Export Citation:

Click for automatic bibliography generation Help

Assignee:

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

International Classes:

C12N15/09; C07K14/435; C07K14/805; C12N15/12; C12P19/34

Domestic Patent References:

JP2003503072A	2003-01-28
JP2002500013A	2002-01-08
JPS61267599A	1986-11-27

Foreign References:

WO2001092320A2

2001-12-06

Attorney, Agent or Firm:

Hajime Tsukuni
Fumio Shinoda

Previous Patent: 間葉幹細胞由来の血液生成物

Next Patent: 二つのキャビネットを備えた実験室用装置