Title:
クローン原性内皮前駆細胞の単離、増殖および使用
Document Type and Number:
Japanese Patent JP2007521817
Kind Code:
A
Abstract:
A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential—endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived from cord blood, or from HUVECs and HAECs. EPCs were found to reside in vessel walls. Use of a feeder layer of cells derived from high proliferative potential-endothelial colony forming cells (HPP-ECPCS) from human umbilical cord blood, stimulates growth and survival of repopulating hematopoietic stem and progenitor cells. Stimulation of growth and survival was determined by increased numbers of progenitor cells in in vitro cultures and increased levels of human cell engraftment in the NOD/SCID immunodeficient mouse transplant system.
Inventors:
Yoder, Marvin Sea.
Ingram, David A.
Ingram, David A.
Application Number:
JP2006552372A
Publication Date:
August 09, 2007
Filing Date:
February 09, 2005
Export Citation:
Assignee:
INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION
International Classes:
A61K35/28; A61K35/44; A61L27/00; A61P43/00; C12N5/074; C12N5/0789; C12Q1/02; A61K35/12
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita