Title:
内分泌攪乱化学物質のスクリーニングのためのステロイド産生改変細胞および方法
Document Type and Number:
Japanese Patent JP2013504333
Kind Code:
A
Abstract:
An isolated steroidogenesis modified cell comprising one or more steroid biosynthesis knock down nucleic acid operatively linked to a promoter, wherein the steroid biosynthesis knock down nucleic acid reduces the expression of a gene selected from the group CYP21A2, CYP11A1, CYP17A1, CYP19A1, 3-betaHSD1, 3-betaHSD2, 17-betaHSD1, StAR, HMGR, CYP11B2, CYP11B1, 5alpha-Reductase 2, SULT1E1, CYP3A4 and UTG1A1, wherein the cell comprises reduced expression of one or more of said genes. The cells are useful for identifying endocrine disruptors. Accordingly, the disclosure includes in a further aspect a screening assay for identifying an endocrine disruptor comprising: a) contacting a cell described herein with a test substance; b) determining a level of at least one steroid or steroidogenic gene mRNA or enzyme activity; wherein a modulation in the level of the at least one steroid or steroidogenic gene mRNA or enzyme activity compared to a control is indicative that the test substance is an endocrine disruptor.
Inventors:
Tsang, Xiao Wei
Marx, Hecker
John, Pee Guigi
Marx, Hecker
John, Pee Guigi
Application Number:
JP2012529078A
Publication Date:
February 07, 2013
Filing Date:
September 16, 2010
Export Citation:
Assignee:
University of Saskatchewan
International Classes:
C12N15/09; C12N5/10; C12Q1/02
Attorney, Agent or Firm:
Hirohito Katsunuma
Yukitaka Nakamura
Noritaka Yokota
Takeyasu Ito
Yukitaka Nakamura
Noritaka Yokota
Takeyasu Ito