Title:
高解像能融解の較正の改善
Document Type and Number:
Japanese Patent JP2016504041
Kind Code:
A
Abstract:
本発明は高解像能融解ＰＣＲ実験における温度較正を行うための方法及びキットに関する。本発明は、更に、標的及び較正物質に対して同一又は同様の融解温度の読み出しを可能にする最適な較正方法に関する。本明細書は、更に前記方法を行う装置及び前記方法を実行するコンピュータプログラムに関する。【選択図】なし
Inventors:
Riser, astrito
Gregnor, Zagner
Gregnor, Zagner
Application Number:
JP2015552042A
Publication Date:
February 12, 2016
Filing Date:
January 08, 2014
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12N15/09; C12M1/00; C12Q1/68
Domestic Patent References:
| JP2011519570A | 2011-07-14 | |||
| JPH11155598A | 1999-06-15 | |||
| JP2010504745A | 2010-02-18 |
Other References:
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IDAHO TECHNOLOGY INC: "Small amplicon gentyping using internal temperature calibration and high-resolution melting", BIOTECHNIQUES, vol. 44, no. 4, JPN6017025443, 2008, pages 577 - 578, XP002695209, ISSN: 0003735950, DOI: 10.2144/000112882
MARRAS SAE: "Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes", METHODS IN MOLECULAR BIOLOGY: FLUORESCENT ENERGY TRANSFER NUCLEIC ACID PROBES: DESIGNS AND PROTOCOLS, vol. 335, JPN6017025444, 2006, pages 3 - 16, XP055193619, ISSN: 0003735951, DOI: 10.1385/1-59745-069-3:3
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IDAHO TECHNOLOGY INC: "Small amplicon gentyping using internal temperature calibration and high-resolution melting", BIOTECHNIQUES, vol. 44, no. 4, JPN6017025443, 2008, pages 577 - 578, XP002695209, ISSN: 0003735950, DOI: 10.2144/000112882
MARRAS SAE: "Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes", METHODS IN MOLECULAR BIOLOGY: FLUORESCENT ENERGY TRANSFER NUCLEIC ACID PROBES: DESIGNS AND PROTOCOLS, vol. 335, JPN6017025444, 2006, pages 3 - 16, XP055193619, ISSN: 0003735951, DOI: 10.1385/1-59745-069-3:3
ZHOU L ET AL: "High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution", CLINICAL CHEMISTRY, vol. 51, no. 10, JPN6017025447, 2005, pages 1770 - 1777, XP008118572, ISSN: 0003735952, DOI: 10.1373/clinchem.2005.054924
Attorney, Agent or Firm:
Shinjiro Ono
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto